ABCB9 Antibody

Western Blot: ABCB9 Antibody [NBP1-18886] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20%of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50ug) cells. Antibodies: Affinity purified rabbit anti-ABCB9 antibody used for WB at 0.04 ug/ml (A) and 1ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Immunohistochemistry-Paraffin: ABCB9 Antibody [NBP1-18886] - Human prostate carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ABCB9 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 475 and 525 of human ATP-binding cassette, sub-family B (MDR/TAP), member 9 using the numbering given in entry NP_062571.1 (GeneID 23457).
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABCB9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 2-5 ug/mg
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 or Tris-EDTA pH9.0 is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ABCB9 Antibody

• ABC transporter 9 protein
• ATP-binding cassette sub-family B member 9
• ATP-binding cassette transporter 9
• ATP-binding cassette, sub-family B (MDR/TAP), member 9
• EC 3.6.3
• EC 3.6.3.44
• EST122234
• hABCB9
• KIAA1520
• TAPL
• TAP-like protein

Background

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. The function of this half-transporter has not yet been determined; however, this protein may play a role in lysosomes. Alternative splicing of this gene results in distinct isoforms which are likely to have different substrate specifications. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for ABCB9 Antibody (NBP1-18886). (Showing 1 - 1 of 1 FAQ).

1. I would like to use this product for IHC on paraffin. Which kind of antigen retrieval is the best for this product?
   • In our testing of NBP1-18886 in IHC-P, we used the Citrate Buffer pH 6.0 antigen retrieval method.protocol

Bioinformatics

• Gene Symbol: ABCB9
• Entrez:
  • 23457(Human)
• Uniprot:
  • NP_062571.1(Human)