ABCB5 Antibody

Product Discontinued

ABCB5 Antibody Summary

• Immunogen: KLH-conjugated synthetic peptide between amino acids 1-30 from N-terminal region of human ABCB5.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABCB5
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1:50-1:100
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Purity: Ammonium sulfate precipitation

Alternate Names for ABCB5 Antibody

• ABCB5 P-gp
• ABCB5
• ABCB5alpha
• ABCB5beta
• ATP-binding cassette protein
• ATP-binding cassette sub-family B member 5
• ATP-binding cassette, sub-family B (MDR/TAP), member 5
• EC 3.6.3
• EC 3.6.3.44
• EST422562
• P-glycoprotein ABCB5

Background

ATP-Binding Cassette proteins comprise a superfamily of transporters with a wide variety of substrates. ABCB5 is a novel member of the human P-glycoprotein family. It functions as a determinant of membrane potential and regulator of cell fusion in physiologic skin progenitor cells. Cell fusion is thought to contribute to tissue differentiation. ABCB5 is predominantly expressed in melanoma cells and is a novel molecular marker for a distinct subset of chemoresistant stem cell phenotype-expressing tumor cells among human epidermal melanocytes. It has been identified as a novel drug transporter in human malignant melanoma. ABCB5 is a rhodamine efflux transporter. This antibody product is intended to be used to confirm cellular localization and expression level of ABCB5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCB5 Antibody (NBP1-50547)(2)

Publications using NBP1-50547

• Jongkhajornpong P, Nakamura T, Sotozono C et al. Elevated expression of ABCB5 in ocular surface squamous neoplasia Sci Rep 2016-02-04 [PMID: 26843453] (IHC-P, Human)
• Ksander BR, Kolovou PE, Wilson BJ et al. ABCB5 is a limbal stem cell gene required for corneal development and repair. Nature 2014-07-17 [PMID: 25030174] (WB)

FAQs for ABCB5 Antibody (NBP1-50547). (Showing 1 - 1 of 1 FAQs).

1. I am writing in regards to your product 'ABCB5 Antibody (NBP1-50547)'. I am interested in performing FACS analysis with it. I would like to ask you, however, whether the epitope recognized by the antibody is located extracellularly. Or does the antibody recognize an epitope intracellularly and thus for FACS analysis cells should be first permeabilized?
   • The immunogen used to generate NBP1-50547 is located on the N-terminal which for ABCB5 is the extracellular domain.

Bioinformatics

• Gene Symbol: ABCB5
• Entrez:
  • 340273(Human)
• Uniprot:
  • Q2M3G0()