ABCB5 Antibody (5H3C6) - BSA Free

Western Blot: ABCB5 Antibody (5H3C6) [NBP2-22213] - Analysis using ABCB5 mouse mAb against A431 (1) cell lysate.

Immunocytochemistry/Immunofluorescence: ABCB5 Antibody (5H3C6) [NBP2-22213] - Analysis of HepG2 cells using ABCB5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: ABCB5 Antibody (5H3C6) [NBP2-22213] - Analysis of paraffin-embedded breast cancer tissues using ABCB5 mouse mAb with DAB staining.

Flow Cytometry: ABCB5 Antibody (5H3C6) [NBP2-22213] - Analysis of HepG2 cells using ABCB5 mouse mAb (green) and negative control (purple).

Western Blot: ABCB5 Antibody (5H3C6) [NBP2-22213] - Analysis using ABCB5 mAb against human ABCB5 recombinant protein. (Expected MW is 47 kDa)

Immunohistochemistry-Paraffin: ABCB5 Antibody (5H3C6) [NBP2-22213] - Analysis of paraffin-embedded liver cancer tissues using ABCB5 mouse mAb with DAB staining.

ELISA: ABCB5 Antibody (5H3C6) [NBP2-22213] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Product Discontinued

ABCB5 Antibody (5H3C6) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human ABCB5 (AA: 481-674) expressed in E. Coli.
• Localization: Cytoplasm
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABCB5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 89.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 31539544).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for ABCB5 Antibody (5H3C6) - BSA Free

• ABCB5 P-gp
• ABCB5
• ABCB5alpha
• ABCB5beta
• ATP-binding cassette protein
• ATP-binding cassette sub-family B member 5
• ATP-binding cassette, sub-family B (MDR/TAP), member 5
• EC 3.6.3
• EC 3.6.3.44
• EST422562
• P-glycoprotein ABCB5

Background

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCB5 Antibody (NBP2-22213)(1)

Publication using NBP2-22213

• Naranjo A, Pelaez D, Arrieta E et al. Cellular and molecular assessment of rose bengal photodynamic antimicrobial therapy on keratocytes, corneal endothelium and limbal stem cell niche Exp. Eye Res. 2019-09-17 [PMID: 31539544] (ICC/IF, IHC/IF, Rabbit)

FAQs for ABCB5 Antibody (NBP2-22213). (Showing 1 - 1 of 1 FAQ).

1. What is the protein sequence of the ABCB5 antibody NBP2-22213 (AA: 481-674)?
   • The immunogen refers to amino acids 481-674 of isoform 1 (ABCB5beta). The protein sequence of human ABCB5 can be found on an excellent protein database called UniProt.  http://www.uniprot.org/uniprot/Q2M3G0#sequences. HAFIYFAYAA GFRFGAYLIQ AGRMTPEGMF IVFTAIAYGA MAIGETLVLA PEYSKAKSGA AHLFALLEKK  PNIDSRSQEG KKPDTCEGNL EFREVSFFYP CRPDVFILRG LSLSIERGKT VAFVGSSGCG KSTSVQLLQR LYDPVQGQVL FDGVDAKELN VQWLRSQIAI VPQEPVLFNC SIAENIAYGD NSRV

Bioinformatics

• Gene Symbol: ABCB5