AAK1 Antibody

Images

 
Western Blot: AAK1 Antibody [NBP1-77335] - A-20 lysate with Aak1 antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: AAK1 Antibody [NBP1-77335] - A-20 cells with AAK1 antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

AAK1 Antibody Summary

Immunogen
Antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of the human Aak1. The immunogen is located within amino acids 190 - 240 of Aak1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AAK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Control Peptide
AAK1 Peptide (NBP1-77335PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AAK1 Antibody

  • AAK1
  • Adaptor-associated kinase 1
  • AP2 associated kinase 1
  • AP2-associated protein kinase 1
  • DKFZp686F03202
  • DKFZp686K16132
  • EC 2.7.11.1
  • FLJ23712
  • FLJ25931
  • FLJ31060
  • FLJ45252
  • KIAA1048FLJ42882
  • MGC138170
  • MGC164568
  • MGC164570

Background

AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxphi) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist; the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AAK1
Entrez
Uniprot