AADACL1 Antibody

Product Discontinued

AADACL1 Antibody Summary

• Immunogen: Peptide with sequence C-RTRNSYIKWLDQN corresponding to C-Terminus according to NP_065843.3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NCEH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunofluorescence
  • Peptide ELISA Detection limit 1:1000
• Application Notes: WB: Preliminary experiments gave an approx. 26-28 kDa band in human brain (amygdala, cerebellum, hippocampus) lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 49.1 kDa band according to NP_065843.3. The 26-28 kDa band was successfully blocked by incubation with the immunizing peptide.
• Control:

  AADACL1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for AADACL1 Antibody

• AADACL1
• EC 3.1.1.-
• EC 3.1.1.71
• EC 3.1.1.79
• KIAA1363
• KIAA1363EC 3.1.1
• NCEH
• NCEH1
• NCEHArylacetamide deacetylase-like 1EC 3.1.1.-
• neutral cholesterol ester hydrolase 1

Background

Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for cholesterol ester hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AADACL1 Antibody (NB100-93363)(1)

Publication using NB100-93363

• Chiang KP, Niessen S, Saghatelian A, Cravatt BF. An enzyme that regulates ether lipid signaling pathways in cancer annotated by multidimensional profiling. Chem Biol 2006-10-01 [PMID: 17052608]

Bioinformatics

• Gene Symbol: NCEH1
• Entrez:
  • 57552(Human)
  • 320024(Mouse)