A-RAF Antibody (684128) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: WB
• Clone: 684128
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

A-RAF Antibody (684128) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: E. coli-derived recombinant human A-Raf
  Asn150-Lys314
  Accession # P10398
• Specificity: Detects human A-Raf in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) B-Raf, rhRaf-1 (aa 1-648), rhRaf-1 (aa 189-353), or rhKSR1 is observed. In Western blots, no cross-reactivity with recombinant human (rh) B-Raf or rhKSR1 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARAF
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for A-RAF Antibody (684128) [CoraFluor™ 1]

• ARaf
• A-Raf
• ARAF1A-RAF
• EC 2.7.11
• EC 2.7.11.1
• Oncogene ARAF1
• PKS
• PKS2
• PKS2A-Raf proto-oncogene serine/threonine-protein kinase
• Proto-oncogene A-Raf
• Proto-oncogene A-Raf-1
• Proto-oncogene Pks
• RAFA1
• Ras-binding protein DA-Raf
• serine/threonine-protein kinase A-Raf
• v-raf murine sarcoma 3611 viral oncogene homolog 1
• v-raf murine sarcoma 3611 viral oncogene homolog

Background

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARAF