A-RAF Antibody (684128) [Alexa Fluor™ Plus 647]

• Reactivity: Hu
• Clone: 684128
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 647

A-RAF Antibody (684128) [Alexa Fluor™ Plus 647] Summary

• Specificity: Detects human A-Raf in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) B-Raf, rhRaf-1 (aa 1-648), rhRaf-1 (aa 189-353), or rhKSR1 is observed. In Western blots, no cross-reactivity with recombinant human (rh) B-Raf or rhKSR1 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for A-RAF Antibody (684128) [Alexa Fluor™ Plus 647]

• ARaf
• A-Raf
• ARAF1A-RAF
• EC 2.7.11
• EC 2.7.11.1
• Oncogene ARAF1
• PKS
• PKS2
• PKS2A-Raf proto-oncogene serine/threonine-protein kinase
• Proto-oncogene A-Raf
• Proto-oncogene A-Raf-1
• Proto-oncogene Pks
• RAFA1
• Ras-binding protein DA-Raf
• serine/threonine-protein kinase A-Raf
• v-raf murine sarcoma 3611 viral oncogene homolog 1
• v-raf murine sarcoma 3611 viral oncogene homolog

Background

The Raf serine/threonine kinases are effectors of Ras that function as MAP3Ks in the ERK phosphorylation cascade. Mammals express three Raf proteins: A-Raf, B-Raf, and Raf-1, also known as C-Raf. Human A-Raf is a 70 kDa protein that shares three regions with B-Raf and Raf-1: aa 14-153 which contains a Ras-binding domain, a Cys-rich domain, and a lipid-binding domain; aa 209-223; and aa 308-573 which contains the Ser/Thr protein kinase domain and a second lipid-binding domain. A-Raf is activated by phosphorylation at Ser 257, 262, and 264. It then regulates multiple processes including endocytic trafficking, glycolysis, cell cycle progression, and apoptosis. Within aa 150-314, human A-Raf shares 87% aa sequence identity with mouse and rat A-Raf.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.