5-Lipoxygenase Antibody

Western Blot: 5-Lipoxygenase Antibody [H00000240-D01P] - Analysis of ALOX5 expression in mouse spleen.

Immunocytochemistry/Immunofluorescence: 5-Lipoxygenase Antibody [H00000240-D01P] - Analysis of antibody to ALOX5 on HeLa cell. Antibody concentration 30 ug/ml.

Western Blot: 5-Lipoxygenase Antibody [H00000240-D01P] - ALOX5 MaxPab rabbit polyclonal antibody. Western Blot analysis of ALOX5 expression in human spleen.

Western Blot: 5-Lipoxygenase Antibody [H00000240-D01P] - Analysis of ALOX5 expression in C32.

Western Blot: 5-Lipoxygenase Antibody [H00000240-D01P] - Analysis of ALOX5 expression in transfected 293T cell line by ALOX5 polyclonal antibody.Lane 1: ALOX5 transfected lysate(78.00 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

5-Lipoxygenase Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: ALOX5 (NP_000689.1, 1 a.a. - 674 a.a.) full-length human protein. MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFERGAVDSYDVTVDEELGEIQLVRIEKRKYWLNDDWYLKYITLKTPHGDYIEFPCYRWITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNGCNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDPCTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVQMVQRAMKDLTYASLCFPEAIKARGMESKEDIPYYFYRDDGLLVWEAIRTFTAEVVDIYYEGDQVVEEDPELQDFVNDVYVYGMRGRKSSGFPKSVKSREQLSEYLTVVIFTASAQHAAVNFGQYDWCSWIPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMARFRKNLEAIVSVIAERNKKKQLPYYYLSPDRIPNSVAI
• Specificity: Reacts with arachidonate 5-lipoxygenase.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALOX5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: This antibody is reactive against tissue and transfected lysate in WB and as a detection antibody in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for 5-Lipoxygenase Antibody

• 5Lipoxygenase
• 5-Lipoxygenase
• 5-LO
• 5-LOX
• 5LPG
• ALOX5
• arachidonate 5-lipoxygenase
• arachidonic 5-lipoxygenase alpha-10 isoform
• arachidonic 5-lipoxygenase delta-10-13 isoform
• arachidonic 5-lipoxygenase delta-13 isoform
• arachidonic 5-lipoxygenase delta-p10 isoform
• arachidonic acid 5-lipoxygenase
• EC 1.13.11
• EC 1.13.11.34
• leukotriene A4 synthase
• LOG5
• LOX5A
• MGC163204

Background

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALOX5
• Entrez:
  • 240(Human)
• Uniprot:
  • NP_000689.1(Human)