4EBP1 Antibody (1E2E7) - BSA Free

Immunocytochemistry/Immunofluorescence: 4EBP1 Antibody (1E2E7) [NBP2-61900] - Analysis of Hela cells using Phospho-4E-BP1 (Ser65) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry: 4EBP1 Antibody (1E2E7) [NBP2-61900] - Analysis of paraffin-embedded bladder cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.

Flow Cytometry: 4EBP1 Antibody (1E2E7) [NBP2-61900] - Analysis of Jurkat cells using Phospho-4E-BP1 (Ser65) mouse mAb (green) and negative control (red).

Immunohistochemistry: 4EBP1 Antibody (1E2E7) [NBP2-61900] - Analysis of paraffin-embedded esophageal cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.

ELISA: 4EBP1 Antibody (1E2E7) [NBP2-61900] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IHC
• Clone: 1E2E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

4EBP1 Antibody (1E2E7) - BSA Free Summary

• Immunogen: Synthesized peptide of human 4EBP1 (pSer65).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EIF4EBP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
• Theoretical MW: 12.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for 4EBP1 Antibody (1E2E7) - BSA Free

• 4EBP1
• 4E-BP1
• eIF4E-binding protein 1
• EIF4EBP1
• eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1
• eukaryotic translation initiation factor 4E-binding protein 1
• PHAS-I
• PHAS-IMGC4316
• Phosphorylated heat- and acid-stable protein regulated by insulin 1

Background

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF4EBP1