15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free

Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15 Lipoxygenase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15 L

Immunocytochemistry/Immunofluorescence: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining in COS7 cells

Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Human breast tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.

Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ALOX15 monoclonal antibody.(1:200)

Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Analysis of extracts (35ug) from 9 different cell lines by using anti-15 Lipoxygenase 1 monoclonal antibody.

Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.

Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.

Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.

Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.

Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.

Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.

• Reactivity: Hu, Rt, Ca, Pm, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI3G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free (NBP2-01740) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human ALOX15(NP_001131) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALOX15
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 74.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free

• 12/15-lipoxygenase
• 12-LOX
• 15-lipooxygenase-1
• 15-Lipoxygenase 1
• 15-LOX
• 15-LOX-1
• 15LOX-1
• ALOX15
• arachidonate 15-lipoxygenase
• Arachidonate omega-6 lipoxygenase
• EC 1.13.11
• EC 1.13.11.12
• EC 1.13.11.33
• LOG15
• LOG15,15-LOX

Background

Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as wellas on linoleic acid

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALOX15