15-Lipoxygenase 1 Antibody (3D8) - Azide and BSA Free

Western Blot: 15-Lipoxygenase 1 Antibody (3D8) [H00000246-M04] - Analysis of ALOX15 expression in Hela S3 NE (Cat # L013V3).

Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (3D8) [H00000246-M04] - Analysis of monoclonal antibody to ALOX15 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 1.5 ug/ml

ELISA: 15-Lipoxygenase 1 Antibody (3D8) [H00000246-M04] - Detection limit for recombinant GST tagged ALOX15 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

15-Lipoxygenase 1 Antibody (3D8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ALOX15 (AAH29032, 1 a.a. ~ 662 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGLYRIRVSTGASLYAGSNNQVQLWLVGQHGEAALGKRLWPARGKETELKVEVPEYLGPLLFVKLRKRHLLKDDAWFCNWISVQGPGAGDEVRFPCYRWVEGNGVLSLPEGTGRTVGEDPQGLFQKHREEELEERRKLYRWGNWKDGLILNMAGAKLYDLPVDERFLEDKRVDFEVSLAKGLADLAIKDSLNVLTCWKDLDDFNRIFWCGQSKLAERVRDSWKEDALFGYQFLNGANPVVLRRSAHLPARLVFPPGMEELQAQLEKELEGGTLFEADFSLLDGIKANVILCSQQHLAAPLVMLKLQPDGKLLPMVIQLQLPRTGSPPPPLFLPTDPPMAWLLAKCWVRSSDFQLHELQSHLLRGHLMAEVIVVATMRCLPSIHPIFKLIIPHLRYTLEINVRARTGLVSDMGIFDQIMSTGGGGHVQLLKQAGAFLTYSSFCPPDDLADRGLLGVKSSFYPQDALRLWEIIYRYVEGIVSLHYKTDVAVKDDPELQTWCREITEIGLQGAQDRGFPVSLQARDQVCHFVTMCIFTCTGQHASVHLGQLDWYSWVPNAPCTMRLPPPTTKDATLETVMATLPNFHQASLQMSITWQLGRRQPVMVAVGQHEEEYFSGPEPKAVLKKFREELAALDKEIEIRNAKLDMPYEYLRPSVVENSVAI
• Specificity: arachidonate 15-lipoxygenase
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALOX15
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for 15-Lipoxygenase 1 Antibody (3D8) - Azide and BSA Free

• 12/15-lipoxygenase
• 12-LOX
• 15-lipooxygenase-1
• 15-Lipoxygenase 1
• 15-LOX
• 15-LOX-1
• 15LOX-1
• ALOX15
• arachidonate 15-lipoxygenase
• Arachidonate omega-6 lipoxygenase
• EC 1.13.11
• EC 1.13.11.12
• EC 1.13.11.33
• LOG15
• LOG15,15-LOX

Background

Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as wellas on linoleic acid

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 15-Lipoxygenase 1 Antibody (H00000246-M04)(4)

Publications using H00000246-M04

• Gregus AM, Dumlao DS, Wei SC et al. Systematic analysis of rat 12/15-lipoxygenase enzymes reveals critical role for spinal eLOX3 hepoxilin synthase activity in inflammatory hyperalgesia. FASEB J 2013-02-04 [PMID: 23382512]
• Calandria JM, Marcheselli VL, Mukherjee PK et al. Selective survival rescue in 15-lipoxygenase-1 deficient retinal pigment epithelial cells by the novel docosahexaenoic acid-derived mediator, neuroprotectin D1. J Biol Chem. 2009-04-29 [PMID: 19403949]
• Blesson CS, Roos N, Stephansson O et al. Expression and localization ofprostaglandin receptors and stromal factors in human cervix?ariations in pregnant and non-pregnant states. Open Journal of Molecular and Integrative Physiology 2013-10-17 (IF/IHC, Human)
• Dobrian AD, Lieb DC, Ma Q et al. Differential expression and localization of 12/15 lipoxygenases in adipose tissue in human obese subjects. Biochem Biophys Res Commun;403(3-4):485-90. 2010-12-17 [PMID: 21094135]

Bioinformatics

• Gene Symbol: ALOX15
• Entrez:
  • 246(Human)
• OMIM:
  • 152392(Human)
• Uniprot:
  • AAH29032(Human)