14-3-3 tau/theta Antibody - BSA Free

Western Blot: 14-3-3 tau/theta Antibody [NBP1-31537] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 theta antibody and a competitor's antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: 14-3-3 tau/theta Antibody [NBP1-31537] - Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE, antibody diluted at 1:30000.

Western Blot: 14-3-3 tau/theta Antibody [NBP1-31537] - Sample(30 ug whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 12% SDS PAGE, antibody diluted at 1:1000.

Western Blot: 14-3-3 tau/theta Antibody [NBP1-31537] - Sample (30 ug of whole cell lysate) A: zebrafish eye B: zebrafish heart 12% SDS PAGE NBP1-31537 diluted at 1:1000

Immunohistochemistry-Paraffin: 14-3-3 tau/theta Antibody [NBP1-31537] - 14-3-3 tau/theta antibody detects 14-3-3 tau/theta protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. 14-3-3 tau/theta antibody (NBP1-31537) diluted at 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

14-3-3 tau/theta Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit 14-3-3 tau/theta Antibody - BSA Free (NBP1-31537) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human 14-3-3 tau/theta. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: YWHAQ
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:30000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for 14-3-3 tau/theta Antibody - BSA Free

• 14-3-3 protein tau
• 14-3-3 protein T-cell
• 14-3-3 protein theta
• 1433 theta
• 14-3-3 theta
• 14-3-3,1C5
• HS1 protein
• HS1
• Protein HS1
• protein tau
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, thetapolypeptide
• YWHAQ

Background

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: YWHAQ