14-3-3 sigma/Stratifin Antibody

Product Discontinued

14-3-3 sigma/Stratifin Antibody Summary

• Immunogen: This antibody is specific for the Middle Region of the target protein.
• Specificity: This product is specific for Human 14-3-3 sigma/Stratifin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SFN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:5000-1:20000
• Application Notes: This antibody is useful in ELISA, Western Blot, Immunohistochemistry-Paraffin and Immunoprecipitation.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for 14-3-3 sigma/Stratifin Antibody

• 14-3-3 protein sigma
• 1433 sigma
• 14-3-3 sigma
• Epithelial cell marker protein 1
• HME1
• SFN
• stratifin
• YWHAS

Background

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 14-3-3 sigma/Stratifin Antibody (24350002)(2)

Publications using 24350002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: SFN
• Entrez:
  • 2810(Human)
• Uniprot:
  • P31947()