14-3-3 sigma/Stratifin Antibody (3C3)

Product Discontinued

14-3-3 sigma/Stratifin Antibody (3C3) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: SFN (AAH00329.1, 1 a.a. - 248 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MERASLIQKAKLAEQAERYEDMAAFMKGAVEKGEELSCEERNLLSVAYKNVVGGQRAAWRVLSSIEQKSNEEGSEEKGPEVREYREKAETELQGVCDTVLGLLDSHLIKEAGDAESRVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFSVFHYEIANSPEEAISLAKTTFDEAMADLHTLSEDSYKDSTLIMQLLRDNLTLWTADNAGEEGGEAPQEPQS
• Specificity: SFN - stratifin
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SFN
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for 14-3-3 sigma/Stratifin Antibody (3C3)

• 14-3-3 protein sigma
• 1433 sigma
• 14-3-3 sigma
• Epithelial cell marker protein 1
• HME1
• SFN
• stratifin
• YWHAS

Background

14-3-3 sigma was identified as an epithelial cell marker. It is part of the 14-3-3 family of proteins in which seven isoforms have been identified: beta, zeta, gamma, eta, epsilon, tau, and sigma. 14-3-3 proteins function as adaptors that bind with a number of partners to mediate various signaling pathways. 14-3-3 sigma is also called stratifin or HME1 and appears to function as a tumor suppressor whose expression can be downregulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis. Alternate names for 14-3-3 sigma include epithelial marker protein 1, SFN and YWHAS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 14-3-3 sigma/Stratifin Antibody (H00002810-M01)(2)

Publications using H00002810-M01

• Sousa MM, Zub KA, Aas PA et al. An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells. PLoS One. 2013-02-06 [PMID: 23405159]
• Kadara H, Behrens C, Yuan P et al. A five-gene and corresponding protein signature for stage-I lung adenocarcinoma prognosis Clin Cancer Res 2011-03-15 [PMID: 21163870] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: SFN
• Entrez:
  • 2810(Human)
• OMIM:
  • 601290(Human)
• Uniprot:
  • AAH00329(Human)