14-3-3 epsilon Antibody (JJ08-40)

Western Blot: 14-3-3 epsilon Antibody (JJ08-40) [NBP2-67834] - Analysis of 14-3-3 epsilon on different lysates using anti-14-3-3 epsilon antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: 293T

Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody (JJ08-40) [NBP2-67834] - Staining 14-3-3 epsilon in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody (JJ08-40) [NBP2-67834] - Analysis of paraffin-embedded mouse placenta tissue using anti-14-3-3 epsilon antibody. Counter stained with hematoxylin.

Flow Cytometry: 14-3-3 epsilon Antibody (JJ08-40) [NBP2-67834] - Analysis of SH-SY-5Y cells with 14-3-3 epsilon antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody (JJ08-40) [NBP2-67834] - Staining 14-3-3 epsilon in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody (JJ08-40) [NBP2-67834] - Staining 14-3-3 epsilon in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody (JJ08-40) [NBP2-67834] - Analysis of paraffin-embedded human colon cancer tissue using anti-14-3-3 epsilon antibody. Counter stained with hematoxylin.

Product Discontinued

14-3-3 epsilon Antibody (JJ08-40) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human 14-3-3 epsilon aa 51-100. (SwissProt: P62258 Human; SwissProt: P62259 Mouse; SwissProt: P62260 Rat)
• Localization: Nucleus, Cytoplasm, Melanosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: YWHAE
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for 14-3-3 epsilon Antibody (JJ08-40)

• 1433 epsilon
• 14-3-3 epsilon
• 14-3-3 protein epsilon
• FLJ45465
• FLJ53559,14-3-3E
• KCIP-1
• MDCR
• MDS
• mitochondrial import stimulation factor L subunit
• protein kinase C inhibitor protein-1
• tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide
• tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilonpolypeptide
• YWHAE

Background

14-3-3 epsilon product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: YWHAE