Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-61640-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97868-0.2mg | |||
| BSA Free | NBP1-97868-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22122SS | |||
| Azide and BSA Free | NBP2-80719 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-113SS | |||
| Azide and BSA Free | NBP2-80853 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2548-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-39100-0.025mg | |||
![Western Blot: SIX2 Antibody (3D7) [H00010736-M01] - Analysis of SIX2 expression in transfected 293T cell line by SIX2 monoclonal antibody (M01), clone 3D7.Lane 1: SIX2 transfected lysate(32.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SIX2-Antibody-3D7-Western-Blot-H00010736-M01-img0008.jpg "Western Blot: SIX2 Antibody (3D7) [H00010736-M01] - Analysis of SIX2 expression in transfected 293T cell line by SIX2 monoclonal antibody (M01), clone 3D7.Lane 1: SIX2 transfected lysate(32.3 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - NCAM1 expression in mouse embryonic kidney organ and hFK serum-free cultures. Mouse embryonic kidney organ culture stained for Ncam1, Six2 and E-cad as indicated. An enlargement of the Ncam/Six2 signal is shown to emphasize the nuclear localization of Six2. White arrow illustrates the absence of Six2 signal in E-cad positive cells. An occurrence of Six2/E-cad positive cells is indicated with the asterisk. Images were obtained using Nikon A1R confocal microscope with and processed in ImageJ/Fiji software. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/cgi/doi/10.1002/emmm.201201584), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SIX2-Antibody-3D7-Immunocytochemistry-Immunofluorescence-H00010736-M01-img0010.jpg)
![Western Blot: SIX2 Antibody (3D7) [H00010736-M01] - Analysis of SIX2 over-expressed 293 cell line, cotransfected with SIX2 Validated Chimera RNAi ( Cat # H00010736-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SIX2 monoclonal antibody (M01), clone 3D7 (Cat # H00010736-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/SIX2-Antibody-3D7-Western-Blot-H00010736-M01-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - Characterization of immunosorted NCAM1+ subpopulation. Double labelling of sorted NCAM1+ cells for NCAM1 and SIX2: NCAM1 with DAPI (upper panel; red and blue channels), NCAM1 with SIX2 (lower panel; red and green channels), indicating both NCAM1+ SIX2+ (arrows) and NCAM1+ SIX2- (arrowheads) cells. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/cgi/doi/10.1002/emmm.201201584), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SIX2-Antibody-3D7-Immunocytochemistry-Immunofluorescence-H00010736-M01-img0011.jpg)
![ELISA: SIX2 Antibody (3D7) [H00010736-M01] - Detection limit for recombinant GST tagged SIX2 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SIX2-Antibody-3D7-ELISA-H00010736-M01-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - Characterization of immunosorted NCAM1+ subpopulationA. RT-PCR analysis of gene expression in NCAM1 cell fractions. GAPDH was used as endogenous control & NCAM1− cell were used as the calibrator sample for RQ calculation (therefore = 1). Data were analysed using SDS 3.2 software & presented as average RQ ± SDEV of three replicates. ***p < 0.001, *p < 0.05 versus NCAM1−.B–D. Immunofluorescence staining of NCAM1+ & NCAM1− subpopulations for E-cadherin (E-cad) (B, red) vimentin (C, green) & SIX2 (D, green). Nuclei stained with Dapi (blue). (B–C) Images were obtained using Olympus DP72 camera attached to Olympus BX51 fluorescence microscope & processed via cellSens standard software, bar represents 200 μm. (D) Images were obtained using Zeiss LSM 510 confocal microscope, bar represents 50 μm.E. Fluorescent quantification of SIX2 immunostaining as represented in (D).F. Double labelling of sorted NCAM1+ cells for NCAM1 & SIX2: NCAM1 with DAPI (upper panel; red & blue channels), NCAM1 with SIX2 (lower panel; red & green channels), indicating both NCAM1+ SIX2+ (arrows) & NCAM1+ SIX2− (arrowheads) cells.G. Graph represents percentage of NCAM1+ SIX2+ cells & NCAM1+ SIX2− cells.H. Clonogenic efficiency of NCAM1+ cells sorted from hFK & cultured in SFM. Data are presented as average CE(%) ± SDEV. **p < 0.01 versus NCAM1−.I. Representative morphology of NCAM1+ & NCAM1− clones. Cells were observed using a Nikon Digital Sight camera attached to a Nikon Eclipse TS100 microscope.J. Clonogenic capacity of hFK cells treated with IMGN901(ADC 55 nM), huN901 (Ab55 nM) or not treated (control). Data are presented as average CE(%) ± SDEV. ***p < 0.001, *p < 0.05 versus control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23996934), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00010736-m01_mouse-monoclonal-six2-antibody-3d7-310202415485112.jpg)
![Immunocytochemistry/ Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - Characterization of immunosorted NCAM1+ subpopulationA. RT-PCR analysis of gene expression in NCAM1 cell fractions. GAPDH was used as endogenous control & NCAM1− cell were used as the calibrator sample for RQ calculation (therefore = 1). Data were analysed using SDS 3.2 software & presented as average RQ ± SDEV of three replicates. ***p < 0.001, *p < 0.05 versus NCAM1−.B–D. Immunofluorescence staining of NCAM1+ & NCAM1− subpopulations for E-cadherin (E-cad) (B, red) vimentin (C, green) & SIX2 (D, green). Nuclei stained with Dapi (blue). (B–C) Images were obtained using Olympus DP72 camera attached to Olympus BX51 fluorescence microscope & processed via cellSens standard software, bar represents 200 μm. (D) Images were obtained using Zeiss LSM 510 confocal microscope, bar represents 50 μm.E. Fluorescent quantification of SIX2 immunostaining as represented in (D).F. Double labelling of sorted NCAM1+ cells for NCAM1 & SIX2: NCAM1 with DAPI (upper panel; red & blue channels), NCAM1 with SIX2 (lower panel; red & green channels), indicating both NCAM1+ SIX2+ (arrows) & NCAM1+ SIX2− (arrowheads) cells.G. Graph represents percentage of NCAM1+ SIX2+ cells & NCAM1+ SIX2− cells.H. Clonogenic efficiency of NCAM1+ cells sorted from hFK & cultured in SFM. Data are presented as average CE(%) ± SDEV. **p < 0.01 versus NCAM1−.I. Representative morphology of NCAM1+ & NCAM1− clones. Cells were observed using a Nikon Digital Sight camera attached to a Nikon Eclipse TS100 microscope.J. Clonogenic capacity of hFK cells treated with IMGN901(ADC 55 nM), huN901 (Ab55 nM) or not treated (control). Data are presented as average CE(%) ± SDEV. ***p < 0.001, *p < 0.05 versus control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23996934), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00010736-m01_mouse-monoclonal-six2-antibody-3d7-310202415482241.jpg)
![Immunocytochemistry/ Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - NCAM1 expression in mouse embryonic kidney organ & hFK serum-free culturesMouse embryonic kidney organ culture stained for Ncam1, Six2 & E-cad as indicated. An enlargement of the Ncam/Six2 signal is shown to emphasize the nuclear localization of Six2. White arrow illustrates the absence of Six2 signal in E-cad positive cells. An occurrence of Six2/E-cad positive cells is indicated with the asterisk. Images were obtained using Nikon A1R confocal microscope with & processed in ImageJ/Fiji software.Morphology of hFK cells cultured in SFM or SCM after 3 days (passage 0 Day 3—left panels) & towards confluence (14 days in SFM or 7 days in SCM—right panels). Distinct borders appear in SFM cultures (arrows) whereas cells with different morphology (arrows) are observed in SCM culture. Cells were observed using a Nikon Digital Sight camera attached to a Nikon Eclipse TS100 microscope.qRT-PCR analysis of gene expression in hFK cells cultured in SFM (three independent replicates). hPRT1 was used as endogenous control & SCM cells were used as the calibrator sample for RQ calculation (therefore = 1). Data were analysed using SDS 3.2 software.Representative FACS analysis of NCAM1 expression in hFK cells cultured in SFM at passage1. Data is presented in a histogram graph showing NCAM1 staining in blue & the isotype controls staining (negative control) in red.Immunofluorescence staining of NCAM1 (red) in total hFK cells cultured in SFM. Nuclei stained with Dapi (blue). Images were obtained using Olympus DP72 camera attached to Olympus BX51 fluorescence microscope & processed via cellSens standard software. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23996934), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00010736-m01_mouse-monoclonal-six2-antibody-3d7-310202415482281.jpg)
![Western Blot: ARF5 Antibody (1B4) [H00000381-M01] - Analysis of ARF5 expression in transfected 293T cell line by ARF5 monoclonal antibody (M01), clone 1B4.Lane 1: ARF5 transfected lysate(20.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ARF5-Antibody-1B4-Western-Blot-H00000381-M01-img0015.jpg "Western Blot: ARF5 Antibody (1B4) [H00000381-M01] - Analysis of ARF5 expression in transfected 293T cell line by ARF5 monoclonal antibody (M01), clone 1B4.Lane 1: ARF5 transfected lysate(20.5 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: ARF5 Antibody (1B4) [H00000381-M01] - Analysis of monoclonal antibody to ARF5 on HeLa cell. Antibody concentration 15 ug/ml.](http://images.novusbio.com/fullsize/ARF5-Antibody-1B4-Immunocytochemistry-Immunofluorescence-H00000381-M01-img0009.jpg)
![Western Blot: ARF5 Antibody (1B4) [H00000381-M01] - ARF5 monoclonal antibody (M01), clone 1B4. Analysis of ARF5 expression in HeLa.](http://images.novusbio.com/fullsize/ARF5-Antibody-1B4-Western-Blot-H00000381-M01-img0011.jpg)
![Western Blot: ARF5 Antibody (1B4) [H00000381-M01] - ARF5 monoclonal antibody (M01), clone 1B4. Analysis of ARF5 expression in PC-12.](http://images.novusbio.com/fullsize/ARF5-Antibody-1B4-Western-Blot-H00000381-M01-img0012.jpg)
![Western Blot: ARF5 Antibody (1B4) [H00000381-M01] - ARF5 monoclonal antibody (M01), clone 1B4. Analysis of ARF5 expression in Raw 264.7.](http://images.novusbio.com/fullsize/ARF5-Antibody-1B4-Western-Blot-H00000381-M01-img0013.jpg)
![Western Blot: ARF5 Antibody (1B4) [H00000381-M01] - ARF5 monoclonal antibody (M01), clone 1B4 Analysis of ARF5 expression in A-431.](http://images.novusbio.com/fullsize/ARF5-Antibody-1B4-Western-Blot-H00000381-M01-img0014.jpg)
![ELISA: ARF5 Antibody (1B4) [H00000381-M01] - Detection limit for recombinant GST tagged ARF5 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ARF5-Antibody-1B4-ELISA-H00000381-M01-img0010.jpg)
![Western Blot: CRALBP Antibody (B2) [NB100-74392] - Analysis of CRALBP antibody on human cell.](http://images.novusbio.com/fullsize/CRALBP-Antibody-B2-Western-Blot-NB100-74392-img0004.jpg "Western Blot: CRALBP Antibody (B2) [NB100-74392] - Analysis of CRALBP antibody on human cell.")
![Immunocytochemistry/Immunofluorescence: CRALBP Antibody (B2) [NB100-74392] - Immunostaining of CRALBP in frozen mouse retina sections.](http://images.novusbio.com/fullsize/CRALBP-Antibody-B2-Immunocytochemistry-Immunofluorescence-NB100-74392-img0003.jpg)
![Simple Western: CRALBP Antibody (B2) [NB100-74392] - iPSC-RPE from all donors express RPE marker proteins. Unlike iPSCs, all iPSC-RPE lines showed robust expression of CRALBP. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29540715/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CRALBP-Antibody-B2-Simple-Western-NB100-74392-img0005.jpg)
![Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Western-Blot-NB110-61640-img0040.jpg "Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.")
![Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody (H9010) [NB110-61640] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Immunocytochemistry-Immunofluorescence-NB110-61640-img0034.jpg)
![Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Immunohistochemistry-NB110-61640-img0044.jpg)
![Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Human cell lysates from various cell lines showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. 15 ug protein each lane. Block: 1.5% BSA for 30 minutes at RT. Primary antibody at 1:1000 for 2 hours at RT. Secondary antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Western-Blot-NB110-61640-img0031.jpg)
![Western Blot: HSP90 beta Antibody (H9010) [NB110-61640] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:1000. Secondary Antibody: HRP Goat Anti-Mouse.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Western-Blot-NB110-61640-img0039.jpg)
![Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:10000 for 12 hours at 4C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x. Inflammatory and epithelial mucosa. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Immunohistochemistry-NB110-61640-img0041.jpg)
![Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Immunohistochemistry-NB110-61640-img0042.jpg)
![Immunohistochemistry: HSP90 beta Antibody (H9010) [NB110-61640] - Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 (NB110-61640). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (NB110-61640) at 1:10000 for 12 hours at 4C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-H9010-Immunohistochemistry-NB110-61640-img0043.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of HSC70/HSP73: Lane 1: MW marker, Lane 2: HSC70/HSP73 (bovine), (recombinant) Lane 3: HSP70/HSP72 (human), (recombinant) Lane 4: 3T3, (Cell Lysate) Lane 5: PC-12, (Cell Lysate), Lane 6: CHO-K1 Cell Lysate, Lane 7: GPC-16 Cell Lysate, Lane 8: HeLa, (Cell Lysate).](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1B5-Western-Blot-NBP1-97868-img0002.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of HSC70/HSP73: Lane 1: MW marker, Lane 2: HSC70/HSP73 (bovine), (recombinant) Lane 3: HSP70/HSP72 (human), (recombinant) Lane 4: 3T3, (Cell Lysate) Lane 5: PC-12, (Cell Lysate), Lane 6: CHO-K1 Cell Lysate, Lane 7: GPC-16 Cell Lysate, Lane 8: HeLa, (Cell Lysate).")
![Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of human spleen tissue stained with HSC70/HSP73, mAb (1B5) at 10ug/ml.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1B5-Immunohistochemistry-Paraffin-NBP1-97868-img0001.jpg)
![Western Blot: Pax2 Antibody (3C7) [H00005076-M01] - Analysis of PAX2 expression in transfected 293T cell line by PAX2 monoclonal antibody (M01), clone 3C7.Lane 1: PAX2 transfected lysate(47.41 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Pax2-Antibody-3C7-Western-Blot-H00005076-M01-img0006.jpg "Western Blot: Pax2 Antibody (3C7) [H00005076-M01] - Analysis of PAX2 expression in transfected 293T cell line by PAX2 monoclonal antibody (M01), clone 3C7.Lane 1: PAX2 transfected lysate(47.41 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Pax2 Antibody (3C7) [H00005076-M01] - Analysis of monoclonal antibody to PAX2 on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 0.5 ug/ml.](http://images.novusbio.com/fullsize/Pax2-Antibody-3C7-Immunohistochemistry-Paraffin-H00005076-M01-img0004.jpg)
![ELISA: Pax2 Antibody (3C7) [H00005076-M01] - Detection limit for recombinant GST tagged PAX2 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Pax2-Antibody-3C7-ELISA-H00005076-M01-img0007.jpg)
![Western Blot: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Whole cell protein from HUVEC was separated on a 7.5% gel polyacrylamide gel and transferred to PVDF membrane. The membrane was probed with anti-CD105 antibody at 2 ug/ml and detected with an anti-mouse HRP labeled secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Western-Blot-NBP2-22122-img0006.jpg "Western Blot: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Whole cell protein from HUVEC was separated on a 7.5% gel polyacrylamide gel and transferred to PVDF membrane. The membrane was probed with anti-CD105 antibody at 2 ug/ml and detected with an anti-mouse HRP labeled secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Human endometrial stromal cells stained with Endoglin/CD105 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Immunocytochemistry-Immunofluorescence-NBP2-22122-img0014.jpg)
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Immunohistochemical staining of endoglin (CD105) in B16F10 murine melanoma tumor model. IHC-P image submitted by a verifed customer review.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Immunohistochemistry-Paraffin-NBP2-22122-img0013.jpg)
![Western Blot: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis using CD105 mAb against HEK293 (1) and CD105 (AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysates.](http://images.novusbio.com/fullsize/CD105-Antibody-3A9-Western-Blot-NBP2-22122-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis of HepG2 cells using CD105 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red).](http://images.novusbio.com/fullsize/CD105-Antibody-3A9-Immunocytochemistry-Immunofluorescence-NBP2-22122-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - HMSC cells stained with anti-CD105 antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Immunocytochemistry-NBP2-22122-img0007.jpg)
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD105-Antibody-3A9-Immunohistochemistry-Paraffin-NBP2-22122-img0005.jpg)
![ELISA: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Red: Control Antigen (100 ng), Purple: Antigen (10 ng), Green: Antigen (50 ng), Blue: Antigen (100 ng).](http://images.novusbio.com/fullsize/CD105-Antibody-3A9-ELISA-NBP2-22122-img0001.jpg)
![Western Blot: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Recombinant human MMP-2 expressed from CHO cells was was separated on a 7.5 % gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-MMP-2 in blocking buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Western-Blot-NB200-113-img0007.jpg "Western Blot: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Recombinant human MMP-2 expressed from CHO cells was was separated on a 7.5 % gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-MMP-2 in blocking buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Western Blot: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Samples: 1) Human Recombinant MMP-2, and 2)Schwann Cell Culture Medium Run on 10% SDS-PAGE, followed by electroblot to nitrocellulose and slot blot immunolabeling. Antibodies: primary - NB200-113, secondary - anti-mouse-biotin. Detection: avidin-HRP. Chromagen: DAB/H202.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Western-Blot-NB200-113-img0009.jpg)
![Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Analysis of a FFPE human breast cancer section using 5 ug/ml conc. of MMP2 antibody (clone 2C1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). MMP2 immunoreactivity was observed in the cytoplasm of the cancer cells and the stromal cells, and in the inter-cellular spaces (secreted MMP2). The cancer cells present in the core of tumor areas were found to be negative for MMP2 staining.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Immunohistochemistry-Paraffin-NB200-113-img0006.jpg)
![Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Analysis of a FFPE human breast cancer section using 5 ug/ml conc. of MMP2 antibody (clone 2C1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This MMP2 antibody generated an expected immunoreactivity of MMP2 protein in the cytoplasm of most of the cancer cells and the stromal cells, and in the inter-cellular spaces (secreted pool of MMP2). Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Immunohistochemistry-Paraffin-NB200-113-img0003.jpg)
![Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Upper image: MMP-2 FITC (green), DAPI (blue)Lower image: phase contrast of human choroid plexus (Alzheimer's disease). Image from verified customer review.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Immunohistochemistry-Paraffin-NB200-113-img0011.jpg)
![Flow Cytometry: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Flow Cytometry: MMP-2 Antibody (2C1) [Alexa Fluor® 405] [NB200-113AF405] - CD4+ T cells were activated for 24h before being fixed and permeabilized to detect MMP-2. Image using the Alexa Fluor 405 form of this antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/MMP-2-Antibody-2C1-Pro-and-Active-Flow-Cytometry-NB200-113-img0012.jpg)
![N/A TNF RII/TNFRSF1B [HRP]](http://images.novusbio.com/fullsize2/DATA_TNF_RII_MRT20_ELISA_1029.jpg "N/A TNF RII/TNFRSF1B [HRP]")
![N/A TNF RII/TNFRSF1B [HRP]](http://images.novusbio.com/fullsize2/TNF_RII_MRT20_ELISA_319.jpg)
![N/A TNF RII/TNFRSF1B [HRP]](http://images.novusbio.com/fullsize2/TNF_RII_MRT20_ELISA_320.jpg)
![Western Blot: Dynein intermediate chain 2 Antibody (1C8) [H00064446-M01] - DNAI2 monoclonal antibody (M01), clone 1C8 Analysis of DNAI2 expression in A-431.](http://images.novusbio.com/fullsize/Dynein-intermediate-chain-2-Antibody-1C8-Western-Blot-H00064446-M01-img0008.jpg "Western Blot: Dynein intermediate chain 2 Antibody (1C8) [H00064446-M01] - DNAI2 monoclonal antibody (M01), clone 1C8 Analysis of DNAI2 expression in A-431.")
![Immunocytochemistry/Immunofluorescence: Dynein intermediate chain 2 Antibody (1C8) [H00064446-M01] - Analysis of monoclonal antibody to DNAI2 on A-431 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Dynein-intermediate-chain-2-Antibody-1C8-Immunocytochemistry-Immunofluorescence-H00064446-M01-img0006.jpg)
![Western Blot: Dynein intermediate chain 2 Antibody (1C8) [H00064446-M01] - Analysis of DNAI2 expression in transfected 293T cell line by DNAI2 monoclonal antibody (M01), clone 1C8.Lane 1: DNAI2 transfected lysate(67 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Dynein-intermediate-chain-2-Antibody-1C8-Western-Blot-H00064446-M01-img0009.jpg)
![Sandwich ELISA: Dynein intermediate chain 2 Antibody (1C8) [H00064446-M01] - Detection limit for recombinant GST tagged DNAI2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Dynein-intermediate-chain-2-Antibody-1C8-Sandwich-ELISA-H00064446-M01-img0007.jpg)
![Western Blot: Synaptopodin Antibody [NBP2-39100] - Analysis of SYNPO in rat brain tissue lysate with SYNPO antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/Synaptopodin-Antibody-Western-Blot-NBP2-39100-img0012.jpg "Western Blot: Synaptopodin Antibody [NBP2-39100] - Analysis of SYNPO in rat brain tissue lysate with SYNPO antibody at (A) 1 and (B) 2 ug/mL.")
![Immunohistochemistry: Synaptopodin Antibody [NBP2-39100] - Mouse brain tissue with SYNPO antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Synaptopodin-Antibody-Immunohistochemistry-NBP2-39100-img0015.jpg)
![Western Blot: Synaptopodin Antibody - BSA Free [NBP2-39100] - mTOR signaling pathway regulated PKM2 expression & podocyte differentiation.a IHC detection of p-S6 in renal cortex during postnatal development. Scale bar=20 μm. b Representative western immunoblot analysis of p-S6 in renal cortex. c Representative western immunoblot analysis of p-S6 in cultured podocyte during differentiation. d Western immunoblot analysis of p-S6 & PKM2 expression in Tsc1fl/fl & Tsc1−/−-podocytes. e Rapamycin caused a significant reduction in the protein levels of p-S6 & PKM2 in cultured podocyte. f Western immunoblot analysis of nephrin & synaptopodin with 10 nM rapamycin treatment for 14 days. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32393782), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-39100_rabbit-polyclonal-synaptopodin-antibody-310202415293341.jpg)
![Western Blot: Synaptopodin Antibody - BSA Free [NBP2-39100] - Metabolomics analysis revealed higher lactate production in differentiated podocytes.a Immunofluorescence staining for nephrin (red), phalloidine for F-actin (green) & DAPI for nuclear (blue) in undifferentiated podocytes (UDPs) or differentiated podocytes (DPs) as indicated (n = 5). Scale bar=5 μm. b Representative western blotting results of nephrin, podocin & synaptopodin confirm the differentiation of podocytes (n = 3). c Representative map of 1H-NMR spectra in the extracellular medium incubated with the presence of different podocytes (n = 6). UDM: undifferentiated podocyte medium; DM: differentiated podocyte medium. The map shows the significance of metabolites variations between these two classes. Peaks in the positive direction indicate metabolites that are more abundant in the UDM groups. Consequently, metabolites that are more abundant in DM are presented as peaks in the negative direction. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32393782), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-39100_rabbit-polyclonal-synaptopodin-antibody-310202416113510.jpg)
![Immunocytochemistry/ Immunofluorescence: Synaptopodin Antibody - BSA Free [NBP2-39100] - Immunofluorescence of Synaptopodin in human brain tissue with Synaptopodin antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-39100_rabbit-polyclonal-synaptopodin-antibody-2711202419251591.jpg)
![Immunohistochemistry: Synaptopodin Antibody - BSA Free [NBP2-39100] - Immunohistochemistry of Synaptopodin in human brain tissue with Synaptopodin antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp2-39100_rabbit-polyclonal-synaptopodin-antibody-2711202420405589.jpg)
![Immunocytochemistry/ Immunofluorescence: Synaptopodin Antibody - BSA Free [NBP2-39100] - Immunofluorescence of Synaptopodin in mouse brain tissue with Synaptopodin antibody at 20 u/mL.Red: Synaptopodin Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-39100_rabbit-polyclonal-synaptopodin-antibody-271120242092058.gif)