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Connexin 43/GJA1 Antibody

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Summary
Product Discontinued
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    • Catalog Number
      NBP1-67530
    • Availability
      Product Discontinued

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Connexin 43/GJA1 Antibody Summary

Immunogen
The antiserum was produced against synthesized peptide derived from human Connexin 43. The immunogen region is: C-terminal.
Marker
Gap Junctions Marker
Specificity
Connexin 43 Antibody detects endogenous levels of total Connexin 43 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
GJA1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Adrenal Membrane Tissue Lysate (Adult Membrane Normal)
Human Adrenal Whole Tissue Lysate (Adult Whole Normal)
A-431 Membrane Cell Lysate
Human Brain Cerebral Cortex Membrane Tissue Lysate (Adult Membrane Normal)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Connexin 43/GJA1 Antibody

  • Connexin 43
  • connexin-43
  • CX43
  • CX43gap junction protein, alpha-like
  • DFNB38
  • Gap junction 43 kDa heart protein
  • gap junction alpha-1 protein
  • gap junction protein, alpha 1, 43kDa (connexin 43)
  • gap junction protein, alpha 1, 43kDa
  • GJA1
  • GJAL
  • HSS
  • ODD
  • ODDD
  • ODOD
  • SDTY3

Background

The connexins are a group of GAP junction proteins, which form a hexamer to compose a connexon. Clusters of connexons form a GAP junction through which low molecular weight proteins may diffuse from cell to cell. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and GAP junction communication. There is a decrease in GAP junctional communication in Src transformed cells. The decreased communication appears to be associated with tyrosine phosphorylation of connexin 43. Activated c-Src phosphorylates the C-terminal tail of connexin 43 on residue Tyr 265, resulting in a stable interaction between both proteins leading to inhibition of GAP junctional communication. In addition to tyrosine phosphorylation, connexin 43 has also been shown to be phosphorylated on serine in the absence of Src kinases and on both serine and tyrosine in cells expressing Src kinases such as pp60v-Src and/or c-Src.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
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FAQs for Connexin 43/GJA1 Antibody (NBP1-67530). (Showing 1 - 1 of 1 FAQ).

  1. Can I use Connexin 43/GJA1 Antibody (3E5) in immunohistochemistry for rat ?
    • I have taken a look at this particular clone however we have not tested this in IHC or in rat. The homology with rat is 97.0% which is good however we do not know if this will work in IHC. Please see this antibody NBP1-67530. This has been validated for both IHC and Rat. Another product, NB100-91717, has been validated for Rat and IHC-Paraffin.

Control Lysate(s)

Human Adrenal Membrane Tissue Lysate (Adult Membrane Normal)
Human Adrenal Whole Tissue Lysate (Adult Whole Normal)
A-431 Membrane Cell Lysate
Human Brain Cerebral Cortex Membrane Tissue Lysate (Adult Membrane Normal)

Secondary Antibodies

 

Isotype Controls

Additional Connexin 43/GJA1 Products

Research Areas for Connexin 43/GJA1 Antibody (NBP1-67530)

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Blogs on Connexin 43/GJA1.

Check out the latest blog posts on Connexin 43/GJA1.
Connexin: Bridging the Gap of Intercellular Communication
Connexin 43/GJA1 is a member of the connexin gene family and the most abundant protein component found within gap junctions. Gap junctions are the cell-to-cell contacts that provide direct intercellular communication between cells by regulating back a...  Read full blog post.

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Bioinformatics

Gene Symbol GJA1
Entrez
Uniprot