| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86928-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70362 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71496 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-508SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47861-0.1mg | |||
| IHC-Prediluted | NBP2-48319 | |||
![Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP1-88345] - Staining of human cell line U-2 OS shows localization to nucleus and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88345-img0017.jpg "Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP1-88345] - Staining of human cell line U-2 OS shows localization to nucleus and cytosol. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining in human testis and kidney tissues . Corresponding NCAPH RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0016.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human testis shows positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0014.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human kidney shows no positivity as expected.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human kidney, skin, testis and tonsil using Anti-NCAPH antibody NBP1-88345 (A) shows similar protein distribution across tissues to independent antibody NBP1-88346 (B).](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0020.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human skin shows nuclear positivity in epidermal cells.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0013.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human tonsil shows nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0015.jpg)
![Simple Western: NCAPH Antibody [NBP1-88345] - Simple Western lane view shows a specific band for NCAPH in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Simple-Western-NBP1-88345-img0006.jpg)
![Simple Western: NCAPH Antibody [NBP1-88345] - Electropherogram image(s) of corresponding Simple Western lane view. NCAPH antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).](http://images.novusbio.com/fullsize/NCAPH-Antibody-Simple-Western-NBP1-88345-img0007.jpg)
![Western Blot: SUCLA2 Antibody [NBP1-33015] - Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 10% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/SUCLA2-Antibody-Western-Blot-NBP1-33015-img0009.jpg "Western Blot: SUCLA2 Antibody [NBP1-33015] - Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 10% SDS PAGE diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: SUCLA2 Antibody [NBP1-33015] - SUCLA2 antibody [N2C3] detects SUCLA2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: SUCLA2 protein stained by SUCLA2 antibody [N2C3] diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/SUCLA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33015-img0012.jpg)
![Immunohistochemistry-Paraffin: SUCLA2 Antibody [NBP1-33015] - Mouse stomach. SUCLA2 antibody [N2C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SUCLA2-Antibody-Immunohistochemistry-Paraffin-NBP1-33015-img0010.jpg)
![Western Blot: SUCLA2 Antibody [NBP1-33015] - Sample (20 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:20000.](http://images.novusbio.com/fullsize/SUCLA2-Antibody-Western-Blot-NBP1-33015-img0008.jpg)
![Immunohistochemistry-Paraffin: SUCLA2 Antibody [NBP1-33015] - Cal27 xenograft, using SUCLA2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SUCLA2-Antibody-Immunohistochemistry-Paraffin-NBP1-33015-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SUCLA2 Antibody [NBP1-33015] - Wild-type (WT) and SUCLA2 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLA2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SUCLA2-Antibody-Western-Blot-NBP1-33015-img0011.jpg)
![Western Blot: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Analysis of MRPL12 expression in transfected 293T cell line by MRPL12 monoclonal antibody (M01), clone 3B12-1A3.Lane 1: MRPL12 transfected lysate(21.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MRPL12-Antibody-3B12-1A3-Western-Blot-H00006182-M01-img0014.jpg "Western Blot: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Analysis of MRPL12 expression in transfected 293T cell line by MRPL12 monoclonal antibody (M01), clone 3B12-1A3.Lane 1: MRPL12 transfected lysate(21.3 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Analysis of monoclonal antibody to MRPL12 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MRPL12-Antibody-3B12-1A3-Immunocytochemistry-Immunofluorescence-H00006182-M01-img0008.jpg)
![Immunohistochemistry-Paraffin: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Analysis of monoclonal antibody to MRPL12 on formalin-fixed paraffin-embedded human breast cancer tissue. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/MRPL12-Antibody-3B12-1A3-Immunohistochemistry-Paraffin-H00006182-M01-img0010.jpg)
![Western Blot: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Analysis of MRPL12 over-expressed 293 cell line, cotransfected with MRPL12 Validated Chimera RNAi ( Cat # H00006182-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MRPL12 monoclonal antibody (M01), clone 3B12-1A3 (Cat # H00006182-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/MRPL12-Antibody-3B12-1A3-Western-Blot-H00006182-M01-img0011.jpg)
![Western Blot: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - MRPL12 monoclonal antibody (M01), clone 3B12-1A3 Analysis of MRPL12 expression in COLO 320 HSR.](http://images.novusbio.com/fullsize/MRPL12-Antibody-3B12-1A3-Western-Blot-H00006182-M01-img0013.jpg)
![ELISA: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Detection limit for recombinant GST tagged MRPL12 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MRPL12-Antibody-3B12-1A3-ELISA-H00006182-M01-img0012.jpg)
![Western Blot: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and HMGB1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1.0 ug/ml of Rabbit Anti-Human HMGB1 Polyclonal Antibody (Catalog # NB100-2322) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HMGB1 at approximately 30 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Neural-Stem-Cell-Marker-Antibody-Pack-Knockout-Validated-NBP1-42826-img0018.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and HMGB1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1.0 ug/ml of Rabbit Anti-Human HMGB1 Polyclonal Antibody (Catalog # NB100-2322) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HMGB1 at approximately 30 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry Free-Floating: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - Analysis of rat cerebellum section stained with mouse GFAP mAb (NBP1-05197), dilution 1:1,000 (Green), costained with rabbit neurofilament NF-L pAb, dilution 1:2,000 (Red). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with antibodies. The GFAP antibody stains a network of astroglial cells, while the NF-L antibody labels neuronal cells and their processes.](http://images.novusbio.com/fullsize/Neural-Stem-Cell-Marker-Antibody-Pack-Immunohistochemistry-Free-Floating-NBP1-42826-img0019.jpg)
![Simple Western: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - Simple Western lane view shows lysates of rat cortical neurons, loaded at 0.2 mg/mL. A specific band was detected for beta -III Tubulin at approximately 56 kDa (as indicated) using 10 ug/mL of Mouse Anti-Neuron-specific beta -III Tubulin Monoclonal Antibody (Catalog # MAB1195). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Neural-Stem-Cell-Marker-Antibody-Pack-Simple-Western-NBP1-42826-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - beta -III Tubulin in Differentiated Human Neural Progenitor Cells. beta -III Tubulin was detected in immersion fixed differentiated human neural progenitor cells using Neuron-specific beta -III Tubulin Monoclonal Antibody (clone TuJ-1) (Catalog # MAB1195) for 3 hours at room temperature. Cells were stained (green) and counterstained (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.](http://images.novusbio.com/fullsize/Neural-Stem-Cell-Marker-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NBP1-42826-img0013.jpg)
![Western Blot: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - Detection of SOX2 in mouse brain lysate using NB110-37235 (0.5ug/ml).](http://images.novusbio.com/fullsize/Neural-Stem-Cell-Marker-Antibody-Pack-Western-Blot-NBP1-42826-img0003.jpg)
![Western Blot: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - Western blot shows lysates of human brain (cerebellum) tissue, human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and mouse brain (stem) tissue. PVDF membrane was probed with 0.2 ug/mL of Mouse Anti-Neuron-specific beta -III Tubulin Monoclonal Antibody (Catalog # MAB1195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta -III Tubulin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/Neural-Stem-Cell-Marker-Antibody-Pack-Western-Blot-NBP1-42826-img0023.jpg)
![Simple Western: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - Simple Western lane view shows a specific band for GFAP in 0.05 mg/ml of Human Brain lysate using NBP1-05197. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Neural-Stem-Cell-Marker-Antibody-Pack-Simple-Western-NBP1-42826-img0020.jpg)
![Neural Stem Cell Marker Antibody Pack [NBP1-42826] - Total protein from SHSY-5Y, MCF7, Neuro2A and HeLa was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-HMGB1 [NB100-2322] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Neural-Stem-Cell-Marker-Antibody-Pack-NBP1-42826-img0022.jpg)
![ELISA: Mouse Osteocalcin ELISA Kit (Colorimetric) [NBP2-68151] - Samples were spiked with high concentrations of Mouse Osteocalcin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-68151_mouse-osteocalcin-elisa-kit-colorimetric-13220241626437.jpg "ELISA: Mouse Osteocalcin ELISA Kit (Colorimetric) [NBP2-68151] - Samples were spiked with high concentrations of Mouse Osteocalcin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Osteocalcin ELISA Kit (Colorimetric) [NBP2-68151] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-68151_mouse-osteocalcin-elisa-kit-colorimetric-4320249115646.jpg)
![Western Blot: TOP2A Antibody [NBP1-86928] - MDA-MB-231 and MCF-7 whole cell lysates were loaded (40 ug/lane). 10% SDS-PAGE. TOP2A Antibody (NBP1-86928) primary antibody at 1:1000, 4C, overnight. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TOP2A-Antibody-Western-Blot-NBP1-86928-img0016.jpg "Western Blot: TOP2A Antibody [NBP1-86928] - MDA-MB-231 and MCF-7 whole cell lysates were loaded (40 ug/lane). 10% SDS-PAGE. TOP2A Antibody (NBP1-86928) primary antibody at 1:1000, 4C, overnight. WB image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: TOP2A Antibody [NBP1-86928] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/TOP2A-Antibody-Immunohistochemistry-Paraffin-NBP1-86928-img0013.jpg)
![Western Blot: CD20 Antibody (OTI4B4) [NBP2-45454] - Expression of CD20 in 5 B cell lymphoma cell lines (Raji, Ly10, Daudi, CCL-155, and Ramos). Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD20-Antibody-OTI4B4-Western-Blot-NBP2-45454-img0007.jpg "Western Blot: CD20 Antibody (OTI4B4) [NBP2-45454] - Expression of CD20 in 5 B cell lymphoma cell lines (Raji, Ly10, Daudi, CCL-155, and Ramos). Image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: MS4A1/CD20 Antibody (4B4) [NBP2-45454] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/MS4A1-CD20-Antibody-4B4-Immunohistochemistry-Paraffin-NBP2-45454-img0004.jpg)
![Western Blot: MS4A1/CD20 Antibody (4B4) [NBP2-45454] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/MS4A1-CD20-Antibody-4B4-Western-Blot-NBP2-45454-img0003.jpg)
![Western Blot: MS4A1/CD20 Antibody (4B4) [NBP2-45454] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MS4A1.](http://images.novusbio.com/fullsize/MS4A1-CD20-Antibody-4B4-Western-Blot-NBP2-45454-img0005.jpg)
![Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1 (1:1000).](http://images.novusbio.com/fullsize/Keap1-Antibody-OTI1B4-Western-Blot-NBP2-03319-img0013.jpg "Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1 (1:1000).")
![Immunocytochemistry/Immunofluorescence: Keap1 Antibody (OTI1B4) [NBP2-03319] - Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY KEAP1 using anti-KEAP1 antibody (green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).](http://images.novusbio.com/fullsize/Keap1-Antibody-OTI1B4-Immunocytochemistry-Immunofluorescence-NBP2-03319-img0014.jpg)
![Immunohistochemistry-Paraffin: Keap1 Antibody (OTI1B4) [NBP2-03319] - Staining of paraffin-embedded Human endometrium tissue using anti-KEAP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Keap1-Antibody-OTI1B4-Immunohistochemistry-Paraffin-NBP2-03319-img0007.jpg)
![Flow Cytometry: Keap1 Antibody (OTI1B4) [NBP2-03319] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KEAP1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Keap1-Antibody-OTI1B4-Flow-Cytometry-NBP2-03319-img0003.jpg)
![Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KEAP1 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)](http://images.novusbio.com/fullsize/Keap1-Antibody-OTI1B4-Western-Blot-NBP2-03319-img0009.jpg)
![Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues used at a 1:200 dilution.](http://images.novusbio.com/fullsize/Keap1-Antibody-OTI1B4-Western-Blot-NBP2-03319-img0010.jpg)
![Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - Analysis of human BEAS2B cells.](http://images.novusbio.com/fullsize/Keap1-Antibody-OTI1B4-Western-Blot-NBP2-03319-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Keap1 Antibody (OTI1B4) [NBP2-03319] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells and KEAP1-Knockout Hela cells were separated by SDS-PAGE and immunoblotted with anti-KEAP1 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-beta-actin antibody as a loading control (1:500).](http://images.novusbio.com/fullsize/Keap1-Antibody-OTI1B4-Western-Blot-NBP2-03319-img0012.jpg)
![Immunohistochemistry-Paraffin: Keap1 Antibody (OTI1B4) [NBP2-03319] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KEAP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Keap1-Antibody-OTI1B4-Immunohistochemistry-Paraffin-NBP2-03319-img0005.jpg)
![Immunohistochemistry-Paraffin: Keap1 Antibody (OTI1B4) [NBP2-03319] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KEAP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Keap1-Antibody-OTI1B4-Immunohistochemistry-Paraffin-NBP2-03319-img0006.jpg)
![Flow Cytometry: Keap1 Antibody (OTI1B4) [NBP2-03319] - Analysis of Hela cells, using anti-KEAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Keap1-Antibody-OTI1B4-Flow-Cytometry-NBP2-03319-img0001.jpg)
![Flow Cytometry: Keap1 Antibody (OTI1B4) [NBP2-03319] - Analysis of Jurkat cells, using anti-KEAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Keap1-Antibody-OTI1B4-Flow-Cytometry-NBP2-03319-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunocytochemistry-Immunofluorescence-NB100-74564-img0005.jpg "Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunohistochemistry-Paraffin: Vimentin Antibody (J144) [NB100-74564] - Biopsies of deparaffinized Human melanoma tissue. Cells were probed without (control) or with or an antibody recognizing Vimentin](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunohistochemistry-Paraffin-NB100-74564-img0003.jpg)
![Western Blot: Vimentin Antibody (J144) [NB100-74564] - Analysis of rat brain cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Western-Blot-NB100-74564-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunocytochemistry-Immunofluorescence-NB100-74564-img0007.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (J144) [NB100-74564] - Both normal and cancer biopsies of deparaffinized Human tonsil tissue.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunohistochemistry-Paraffin-NB100-74564-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (J144) [NB100-74564] - Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Vimentin-Antibody-J144-Immunocytochemistry-Immunofluorescence-NB100-74564-img0006.jpg)
![Western Blot: Ku80/XRCC5 Antibody [NB100-508] - MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954, 4T1, B16-F10, Hep3b and 293T cells lysates (20 ug/lane). 10% SDS-PAGE. KU80 (NB100-508) primary antibody at 1:2000 dilution, 4C, overnight. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-Western-Blot-NB100-508-img0005.jpg "Western Blot: Ku80/XRCC5 Antibody [NB100-508] - MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954, 4T1, B16-F10, Hep3b and 293T cells lysates (20 ug/lane). 10% SDS-PAGE. KU80 (NB100-508) primary antibody at 1:2000 dilution, 4C, overnight. Western blot image submitted by a verified customer review.")
![Western Blot: Ku80/XRCC5 Antibody [NB100-508] - Analysis of Ku80 using NB100-508 at 1:500 dilution on 50 ug of total lysates of A431 (lane 1) and AR42J (lane 2) cells.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-Western-Blot-NB100-508-img0004.jpg)
![Immunoprecipitation: Ku80/XRCC5 Antibody [NB100-508] - Detection of Ku80 in either CHO or A549 nuclear extracts (NE) or cytoplasmic lysates (cyto) using NB 100-508. A Ku80 deficient cell lysate, XRS, was used as a negative control.](http://images.novusbio.com/fullsize/Ku80-XRCC5-Antibody-Immunoprecipitation-NB100-508-img0003.jpg)
![Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human and Mouse CDC20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody NB100-59828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Cdc20-Antibody-Western-Blot-NB100-59828-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human and Mouse CDC20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody NB100-59828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Cdc20-Antibody-Immunohistochemistry-NB100-59828-img0010.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human CDC20 on HeLa whole cell lysate using NB100-59828. CDC20 was also immunoprecipitated by rabbit anti-CDC20 antibody NB100-59827.](http://images.novusbio.com/fullsize/Cdc20-Antibody-Western-Blot-NB100-59828-img0006.jpg)
![Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] - Section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/Cdc20-Antibody-Immunohistochemistry-NB100-59828-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Cdc20 Antibody [NB100-59828] - Detection of human CDC20 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDC20 antibody NB100-59828 (lot NB100-59828-2) used for IP at 6 ug per reaction. CDC20 was also immunoprecipitated by a previous lot of this antibody (lot NB100-59828-1) and rabbit anti-CDC20 antibody NB100-59827. For blotting immunoprecipitated CDC20, NB100-59828 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Cdc20-Antibody-Immunoprecipitation-NB100-59828-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis HeLa cells labeling Nucleolin with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is DAPI (Blue).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunocytochemistry-Immunofluorescence-NBP2-44612-img0008.jpg "Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis HeLa cells labeling Nucleolin with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is DAPI (Blue).")
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) [NBP2-44612] - Human Tonsil stained with Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunohistochemistry-Paraffin-NBP2-44612-img0003.jpg)
![Flow Cytometry: Nucleolin Antibody (364-5) [NBP2-44612] - Flow Cytometry of Human Nucleolin Ag on 293T cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Nucleolin Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Flow-Cytometry-NBP2-44612-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunocytochemistry-Immunofluorescence-NBP2-44612-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleolin Antibody (364-5) [NBP2-44612] - Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunocytochemistry-Immunofluorescence-NBP2-44612-img0007.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) [NBP2-44612] - Human Skin stained with Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunohistochemistry-Paraffin-NBP2-44612-img0002.jpg)
![Immunohistochemistry-Paraffin: Nucleolin Antibody (364-5) [NBP2-44612] - Formalin-fixed paraffin-embedded human Colon Carcinoma stained with AF488 conjugate of Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Immunohistochemistry-Paraffin-NBP2-44612-img0004.jpg)
![Flow Cytometry: Nucleolin Antibody (364-5) [NBP2-44612] - Human Nucleolin Ag on 293T Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled Nucleolin Monoclonal Antibody (364-5).](http://images.novusbio.com/fullsize/Nucleolin-Antibody-364-5-Flow-Cytometry-NBP2-44612-img0001.jpg)