Neural Stem Cell Marker Antibody Pack

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Western Blot: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - Detection of SOX2 in mouse brain lysate using NB110-37235 (0.5ug/ml).
Immunocytochemistry/ Immunofluorescence: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - beta -III Tubulin and Nestin in Rat Cortical Stem Cells. beta -III Tubulin and Nestin were detected in rat cortical stem ...read more
Immunohistochemistry: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - Staining of HMGB1 in mouse liver.
Immunocytochemistry/ Immunofluorescence: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - Visualization of nestin in PC-3 cells using NB300-266. Courtesy of Dolnick Lab, Roswell Park Cancer Institute.
Immunocytochemistry/ Immunofluorescence: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - Mixed neuron-glial cultures stained with NBP1-05197, and chicken polyclonal antibody to neurofilament NF-L NBP1-05219 ...read more
Immunocytochemistry/ Immunofluorescence: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - beta -III Tubulin in Differentiated Human Neural Progenitor Cells. beta -III Tubulin was detected in immersion fixed ...read more
Immunocytochemistry/ Immunofluorescence: Neural Stem Cell Marker Antibody Pack [NBP1-42826] - beta -III Tubulin in Rat Cortical Neurons and GFAP in Rat Astrocytes. beta -III Tubulin was detected in rat cortical neurons ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, Ma, Multi, Pm, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P

Order Details

Neural Stem Cell Marker Antibody Pack Summary

Immunogen
NB100-2322 - A synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200). [UniProt #P09429].
NB110-37235 - A synthetic peptide made to the N-terminal region of human SOX2 protein (within residues 1-100). [Swiss-Prot# P48431].
MAB1195 - Neuron-specific beta-III Tubulin MAb (Clone TuJ-1).
NBP1-05197 - A preparation of purified pig spinal cord GFAP.
NB300-266 - A fragment of the human nestin protein corresponding to residues 1464-1614.
Specificity
NB100-2322: HMGB1 - Oligodendrocyte Marker
NB110-37235: SOX2 - Embryonic Stem Cell Marker
MAB1195: Neuron-specific beta-III Tubulin MAb (Clone TuJ-1)
NBP1-05197: GFAP - Astrocyte Marker
NB300-266: Nestin - Cytoskeleton-Intermediate Filaments Marker
Kit Type
Kit
Purity
Immunogen affinity purified

Applications/Dilutions

Application Notes
NB100-2322 - This antibody is useful for Western blot, where a band is seen ~29 kDa. The antibody is also useful for immunohistochemistry paraffin-embedded tissue. Immunoprecipitation has not been tested.

NB110-37235 - Western blot analysis where a band at ~40 kDa is be seen, representing the SOX2 protein.

NBP1-05197 - Can be used to identify astrocytic cells, their progenitors and their descendents in tissue sections and in tissue culture. The epitope recognized by 5C10 is unusually resistant to aldehyde fixation, and so is ideal of studies of formalin-fixed paraffin-embedded histological specimens. A 55 kDa band can be seen in Western blotting.

NB300-266 - May be used in Western blot where a doublet band is seen at ~220-240 kDa.

Reactivity Notes

NB100-2322 - Reacts with human, mouse, rat, dog, bovine and sheep.
NB110-37235 - Human, sheep and mouse. 92% sequence identity with chicken, 86% sequence identity with Xenopus, and 71% sequence identity with Zebrafish proteins.
MAB1195 - Cross reacts in Multiple species.
NBP1-05197 - Reacts with all mammalian GFAP molecules tested to date, including human.
NB300-266 - Rabbit. Reacts with human nestin protein. It does not cross-react with rodent protein.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Kit Components

Components
  1. MAB1195 (Neuron-specific beta-III Tubulin MAb (Clone TuJ-1)
  2. Neural Stem Cell Marker Antibody Pack
  3. NB300-266: Nestin Antibody (10C2)
  4. NB100-2322: HMGB1/HMG-1 Antibody
  5. NBP1-05197: GFAP Antibody (5C10)
  6. NB110-37235: SOX2 Antibody

Notes

This kit contains full sized vials of the following: MAB1195, NB110-37235, NBP1-05197, NB100-2322, and NB300-266.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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WB Video Protocol
ICC/IF Video Protocol

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