| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50045SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-29622SS | |||
| Azide and BSA Free | NBP2-80604 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25259 | |||
| Azide and BSA Free | NBP2-25259SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48417 | |||
| Azide and BSA Free | NBP3-11458 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-57150SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0370A488-100 | |||
| Alexa Fluor 546 | DDX0370A546-100 | |||
| Alexa Fluor 647 | DDX0370A647-100 | |||
| Biotin | DDX0370B-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80886-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43400-0.5mg | |||
| BSA Free | NBP1-43400-0.05mg | |||
| Azide and BSA Free | NBP2-80815 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-55454SS | |||
| Azide and BSA Free | NBP2-80580 | |||
![Western Blot: ALDH1L1 Antibody (4A12) [NBP2-50045] - Analysis of rat and mouse tissue lysates using mouse ALDH1L1 mAb, dilution 1:5000 (Green): [1] protein standard (Red), rat tissue lysates: [2] heart, [3] liver, [4] kidney, [5] lung, [6] brain, and [7] spinal cord; Also mouse tissue lysates: [8] brain, and [9] spinal cord. The band at 100kDa corresponds to ALDH1L1 protein. In line with published work, ALDH1L1 is a major protein of liver and CNS tissue.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-4A12-Western-Blot-NBP2-50045-img0006.jpg "Western Blot: ALDH1L1 Antibody (4A12) [NBP2-50045] - Analysis of rat and mouse tissue lysates using mouse ALDH1L1 mAb, dilution 1:5000 (Green): [1] protein standard (Red), rat tissue lysates: [2] heart, [3] liver, [4] kidney, [5] lung, [6] brain, and [7] spinal cord; Also mouse tissue lysates: [8] brain, and [9] spinal cord. The band at 100kDa corresponds to ALDH1L1 protein. In line with published work, ALDH1L1 is a major protein of liver and CNS tissue.")
![Immunocytochemistry/Immunofluorescence: ALDH1L1 Antibody (4A12) [NBP2-50045] - Analysis of mixed cortical neuron-glial cell culture from an E20 rat stained with mouse ALDH1L1 mAb, dilution 1:1,000 (Green), and costained with chicken Vimentin pAb, dilution 1:5000 (Red). DAPI staining of nuclear DNA (Blue). The ALDH1L1 antibody produces strong cytoplasmic staining of astrocytic glial cells, while the Vimentin antibody labels the intermediate filament cytoskeleton in fibroblasts and other glial cells. Some cells, presumably developing astrocytes, are stained by both ALDH1L1 and vimentin and so appear yellow.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-4A12-Immunocytochemistry-Immunofluorescence-NBP2-50045-img0005.jpg)
![Western Blot: BST2 Antibody (4F6) [NBP2-29622] - WB analysis of BST2 protein in (A) human heart lysate (B) human ovary lysate and on (C) partial recombinant BST2 protein with monoclonal BST2 antibody (clone 4F6) at a concentration of 1 ug/mL. In the tested lysates, two bands were observed which represents the glycosylated (lower intense bands) and the ubiquitinated (higher weak bands) forms of BST2 protein.](http://images.novusbio.com/fullsize/BST2-Antibody-4F6-Western-Blot-NBP2-29622-img0001.jpg "Western Blot: BST2 Antibody (4F6) [NBP2-29622] - WB analysis of BST2 protein in (A) human heart lysate (B) human ovary lysate and on (C) partial recombinant BST2 protein with monoclonal BST2 antibody (clone 4F6) at a concentration of 1 ug/mL. In the tested lysates, two bands were observed which represents the glycosylated (lower intense bands) and the ubiquitinated (higher weak bands) forms of BST2 protein.")
![Immunohistochemistry-Paraffin: BST2 Antibody (4F6) [NBP2-29622] - Analysis of BST2 protein in a section of human lung cancer (squamous cell carcinoma) using 5 ug/mL concentration of BST2 antibody (clone 4F6). The carcinoma cells depicted strong membrane-cytoplasmic BST2 positivity with more intense staining in the cellular membranes, and a relatively less intense staining pattern was observed in the tumor stroma.](http://images.novusbio.com/fullsize/BST2-Antibody-4F6-Immunohistochemistry-Paraffin-NBP2-29622-img0003.jpg)
![Flow (Cell Surface): BST2 Antibody (4F6) [NBP2-29622] - Flow analysis of Molt4 cells using the BST2 antibody [4F6]. The shaded purple region represents cells alone, green represents the isotype control and pink represents the BST2 antibody.](http://images.novusbio.com/fullsize/BST2-Antibody-4F6-Flow-Cell-Surface-NBP2-29622-img0004.jpg)
![Immunohistochemistry-Paraffin: BST2 Antibody (4F6) [NBP2-29622] - Analysis of BST2 protein in a section of human endometrial carcinoma using 5 ug/mL concentration of BST2 antibody (clone 4F6). The carcinoma cells depicted distinct membrane-cytoplasmic BST2 positivity with more intense staining in the cellular membranes.](http://images.novusbio.com/fullsize/BST2-Antibody-4F6-Immunohistochemistry-Paraffin-NBP2-29622-img0002.jpg)
![Western Blot: TLR2 Antibody (67N8F8) [NBP2-25245] - Analysis of human lymph node tissue using TLR2 antibody at a concentration of 1 ug/ml.](http://images.novusbio.com/fullsize/TLR2-Antibody-67N8F8-Western-Blot-NBP2-25245-img0004.jpg "Western Blot: TLR2 Antibody (67N8F8) [NBP2-25245] - Analysis of human lymph node tissue using TLR2 antibody at a concentration of 1 ug/ml.")
![Immunohistochemistry-Paraffin: TLR2 Antibody (67N8F8) [NBP2-25245] - FFPE human esophageal squamous cell carcinoma stained with TLR2 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/TLR2-Antibody-67N8F8-Immunohistochemistry-Paraffin-NBP2-25245-img0003.jpg)
![Flow (Cell Surface): TLR2 Antibody (67N8F8) [NBP2-25245] - Analysis using the FITC conjugate of NBP2-25259. Surface staining of stable HEK293/hTLR2 cells (red) and vector control cells (green) using TLR2 antibody at 5 ul/10^6 cells. Image using the Azide Free form of this antibody.](http://images.novusbio.com/fullsize/TLR2-Antibody-67N8F8-Flow-Cell-Surface-NBP2-25245-img0005.jpg)
![Western Blot: TLR2 Antibody (67N8F8) [NBP2-25245] - Analysis of (A) TLR2 partial recombinant protein, (B) transfected cell lysate, and (C) control 293T lysate using TLR2 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/TLR2-Antibody-67N8F8-Western-Blot-NBP2-25245-img0001.jpg)
![Flow Cytometry: TLR2 Antibody (67N8F8) [NBP2-25245] - (A) Surface staining of transfected TLR2 HEK293 cells (red) and mock transfected cells (green) using TLR2 antibody at 1 ug/10^6 cells. FITC-conjugated anti-mouse IgG secondary was used for this test. (B) Surface staining of PPI-negative PBMC (monocyte-gated) using TLR2 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells. PE-conjugated anti-mouse IgG secondary was used for this test.](http://images.novusbio.com/fullsize/TLR2-Antibody-67N8F8-Flow-Cytometry-NBP2-25245-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MUC5AC Antibody (CLH2) [NBP2-44455] - Human stomach tissue (FFPE). Antibody at 1:100. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/MUC5AC-Antibody-CLH2-Immunocytochemistry-Immunofluorescence-NBP2-44455-img0003.jpg "Immunocytochemistry/Immunofluorescence: MUC5AC Antibody (CLH2) [NBP2-44455] - Human stomach tissue (FFPE). Antibody at 1:100. ICC/IF image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: MUC5AC Antibody (CLH2) [NBP2-44455] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Antibody (CLH2). Courtesy of Dr. Leonor David, IPATIMUP and Medical Faculty, University of Porto, Portugal.](http://images.novusbio.com/fullsize/MUC5AC-Antibody-CLH2-Immunohistochemistry-Paraffin-NBP2-44455-img0002.jpg)
![Western Blot: GNAL Antibody [NBP1-32225] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GNAL antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GNAL-Antibody-Western-Blot-NBP1-32225-img0011.jpg "Western Blot: GNAL Antibody [NBP1-32225] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GNAL antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry: GNAL Antibody [NBP1-32225] - Mouse brain tissue. Antibody at 1:500. Selective and strong signal in the striatum. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GNAL-Antibody-Immunohistochemistry-NBP1-32225-img0006.jpg)
![Western Blot: GNAL Antibody [NBP1-32225] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GNAL antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GNAL-Antibody-Western-Blot-NBP1-32225-img0009.jpg)
![Immunohistochemistry-Paraffin: GNAL Antibody [NBP1-32225] - NCI-N87 xenograft. GNAL antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/GNAL-Antibody-Immunohistochemistry-NBP1-32225-img0002.jpg)
![Western Blot: LPIN1 Antibody [NB110-57150] - Detection of LPIN1 in 3T3L1 lysate.](http://images.novusbio.com/fullsize/LPIN1-Antibody-Western-Blot-NB110-57150-img0003.jpg "Western Blot: LPIN1 Antibody [NB110-57150] - Detection of LPIN1 in 3T3L1 lysate.")
![Immunohistochemistry: LPIN1 Antibody [NB110-57150] - Staining of LPIN1 in mouse pancreas.](http://images.novusbio.com/fullsize/LPIN1-Antibody-Immunohistochemistry-NB110-57150-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Langerin/CD207 Antibody (205C1) [DDX0370P-100] - Mouse epidermal sheet](http://images.novusbio.com/fullsize/Langerin-CD207-Antibody-205C1-Immunocytochemistry-Immunofluorescence-DDX0370P-100-img0004.jpg "Immunocytochemistry/Immunofluorescence: Langerin/CD207 Antibody (205C1) [DDX0370P-100] - Mouse epidermal sheet")
![Flow Cytometry: Langerin/CD207 Antibody (205C1) [DDX0370P-100] - Murine langerhans cells](http://images.novusbio.com/fullsize/Langerin-CD207-Antibody-205C1-Flow-Cytometry-DDX0370P-100-img0003.jpg)
![Chromatin Immunoprecipitation: Langerin/CD207 Antibody (205C1) [DDX0370P-100] - IF on acetone-fixedear epidermalsheet](http://images.novusbio.com/fullsize/Langerin-CD207-Antibody-205C1-Chromatin-Immunoprecipitation-DDX0370P-100-img0006.jpg)
![Immunohistochemistry-Paraffin: SRGN Antibody [NBP1-80886] - Analysis in human bone marrow and skeletal muscle tissues using NBP1-80886 antibody. Corresponding SRGN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SRGN-Antibody-Immunohistochemistry-Paraffin-NBP1-80886-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SRGN Antibody [NBP1-80886] - Analysis in human bone marrow and skeletal muscle tissues using NBP1-80886 antibody. Corresponding SRGN RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: SRGN Antibody [NBP1-80886] - Analysis in human cell lines A-549 and Caco-2. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/SRGN-Antibody-Western-Blot-NBP1-80886-img0008.jpg)
![Immunohistochemistry-Paraffin: SRGN Antibody [NBP1-80886] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/SRGN-Antibody-Immunohistochemistry-Paraffin-NBP1-80886-img0016.jpg)
![Immunohistochemistry-Paraffin: SRGN Antibody [NBP1-80886] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/SRGN-Antibody-Immunohistochemistry-NBP1-80886-img0012.jpg)
![Immunohistochemistry-Paraffin: SRGN Antibody [NBP1-80886] - Staining of human gastrointestinal shows moderate cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/SRGN-Antibody-Immunohistochemistry-NBP1-80886-img0013.jpg)
![Immunohistochemistry-Paraffin: SRGN Antibody [NBP1-80886] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/SRGN-Antibody-Immunohistochemistry-Paraffin-NBP1-80886-img0015.jpg)
![Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) [NBP1-43400] - Analysis using the FITC conjugate of NBP1-43400. Staining of Human peripheral blood lymphocytes with GoH3 FITC.](http://images.novusbio.com/fullsize/Integrin-alpha-6-CD49f-Antibody-GoH3-Flow-Cytometry-NBP1-43400-img0005.jpg "Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) [NBP1-43400] - Analysis using the FITC conjugate of NBP1-43400. Staining of Human peripheral blood lymphocytes with GoH3 FITC.")
![Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) [NBP1-43400] - Staining of normal human peripheral blood cells with 0.25 ug of Rat IgG2a Isotype Control Purified (NBP1-43400) (open histogram) or 0.25 ug of Anti-Human/Mouse CD49f (Integrin alpha 6) Purified (filled histogram) followed by Anti-Rat IgG PE. Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/Integrin-alpha-6-CD49f-Antibody-GoH3-Flow-Cytometry-NBP1-43400-img0001.jpg)
![Flow Cytometry: Integrin alpha 6/CD49f Antibody (GoH3) [NBP1-43400] - Analysis using the Biotin conjugate of NBP1-43400. Staining of normal human peripheral blood cells with 0.25 ug of Rat IgG2a k Isotype Control Biotin (black) or 0.125 ug of Anti-Human/Mouse CD49f Integrin a6 Biotin (purple) followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/Integrin-alpha-6-CD49f-Antibody-GoH3-Flow-Cytometry-NBP1-43400-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: RAB35 Antibody [NBP2-20042] - RAB35 antibody [N1C3] detects RAB35 protein at Golgi apparatus by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RAB35 stained by RAB35 antibody [N1C3] diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/RAB35-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20042-img0011.jpg "Immunocytochemistry/Immunofluorescence: RAB35 Antibody [NBP2-20042] - RAB35 antibody [N1C3] detects RAB35 protein at Golgi apparatus by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RAB35 stained by RAB35 antibody [N1C3] diluted at 1:500.Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: RAB35 Antibody [NBP2-20042] - Human breast cancer, using RAB35 antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RAB35-Antibody-Immunohistochemistry-Paraffin-NBP2-20042-img0002.jpg)
![Western Blot: RAB35 Antibody [NBP2-20042] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RAB35-Antibody-Western-Blot-NBP2-20042-img0009.jpg)
![Western Blot: RAB35 Antibody [NBP2-20042] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RAB35-Antibody-Western-Blot-NBP2-20042-img0010.jpg)
![Immunohistochemistry-Paraffin: RAB35 Antibody [NBP2-20042] - RAB35 antibody [N1C3] detects RAB35 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.RAB35 stained by RAB35 antibody [N1C3] (NBP2-20042) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20042_rabbit-polyclonal-rab35-antibody-91020242011500.jpg)
![Immunohistochemistry-Paraffin: RAB35 Antibody [NBP2-20042] - RAB35 antibody [N1C3] detects RAB35 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.RAB35 stained by RAB35 antibody [N1C3] (NBP2-20042) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20042_rabbit-polyclonal-rab35-antibody-9102024205618.jpg)
![Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (NBP2-20042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20042_rabbit-polyclonal-rab35-antibody-western-blot-26112025199275.jpg)
![Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (NBP2-20042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20042_rabbit-polyclonal-rab35-antibody-western-blot-261120251837504.jpg)
![Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - Analysis in human serum total protein (lane 2). Lane 1 is a MW standard.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Western-Blot-NB110-55454-img0009.jpg "Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - Analysis in human serum total protein (lane 2). Lane 1 is a MW standard.")
![Immunohistochemistry-Paraffin: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human liver using 5 ug/ml conc. of Apolipoprotein A5 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Apolipoprotein A5 in hepatocytes was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Immunohistochemistry-Paraffin-NB110-55454-img0011.jpg)
![Flow Cytometry: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Apolipoprotein A5 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Flow-Cytometry-NB110-55454-img0007.jpg)
![Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - Analysis of ApoA5 in HepG2 lysates](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Western-Blot-NB110-55454-img0002.jpg)
![Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - Detection of Apo A5 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Western-Blot-NB110-55454-img0008.jpg)
![Western Blot: KPNA3 Antibody [NB100-81650] - Association of NCBP3 and NCBP2 with NCBP1 and proteins involved in RNA processing. Western blot analysis of representative GFP-precipitated proteins identified by AP-MS analysis. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms9192), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KPNA3-Antibody-Western-Blot-NB100-81650-img0004.jpg "Western Blot: KPNA3 Antibody [NB100-81650] - Association of NCBP3 and NCBP2 with NCBP1 and proteins involved in RNA processing. Western blot analysis of representative GFP-precipitated proteins identified by AP-MS analysis. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms9192), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: KPNA3 Antibody [NB100-81650] - Human prostate carcinoma. Antibody used at a dilution of 1:200.](http://images.novusbio.com/fullsize/KPNA3-Antibody-Immunohistochemistry-Paraffin-NB100-81650-img0003.jpg)
![Western Blot: KPNA3 Antibody [NB100-81650] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-81650 used for WB at 0.4 mcg/ml (A and B) and used for IP at 3 mcg/mg lysate.](http://images.novusbio.com/fullsize/KPNA3-Antibody-Western-Blot-NB100-81650-img0002.jpg)
![Western Blot: KPNA3 Antibody [NB100-81650] - DZNep suppressed MPNST724 xenograft tumor initiation & growthin vivo. (A) MPNST724 xenograft tumor groups treated with vehicle only, 1 mg/kg DZNep, or 5 mg/kg DZNep 13 weeks after subcutaneous injection (n = 9 mice per group). (B) Growth curves of tumors treated with vehicle only, 1 mg/kg DZNep, or 5 mg/kg DZNep over a period of 13 weeks. Mean ± SD values are shown; *p < 0.05, Student t test. (C) Western blot analyses of EZH2, KPNB1, & actin in tumor MPNST samples treated with vehicle only, 1 mg/kg DZNep, or 5 mg/kg DZNep. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/s12943-015-0325-1), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-81650_rabbit-polyclonal-kpna3-antibody-310202415392575.jpg)
![Western Blot: KPNA3 Antibody [NB100-81650] - DZNep inhibited EZH2/miR-30d/KPNB1 signaling in MPNST cells. (A) Western blot analyses of EZH2, cleaved PARP (cPARP), KPNB1, & GAPDH (loading control) in MPNST724 & S462 cells treated with DZNep at increasing concentrations for 48 hours. (B) qRT-PCR analyses of miR-30d expression in MPNST724 & S462 cells treated with DZNep for 96 hours. miR-30d expression was normalized to SNORD47. Mean ± SD values are shown (n = 3); *p < 0.05, Student t test. (C) Promoter activity assay showed that DZNep treatment increased miR-30d promoter activity in S462 cells. Mean ± SD values are shown (n = 3); *p < 0.05; Student t test. (D) Luciferase reporter assay showed that DZNep treatment inhibited miR-30d target reporter activity in S462 cells. Mean ± SD values are shown (n = 3); **p < 0.01, Student t test. (E) Luciferase reporter assay indicated that DZNep treatment inhibited wild-type (WT) KPNB1 3’UTR reporter activity but not mutant (MT) KPNB1 3’UTR reporter activity in S462 cells. Mean ± SD values are shown (n = 3); *p < 0.05, Student t test. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/s12943-015-0325-1), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-81650_rabbit-polyclonal-kpna3-antibody-310202415532089.jpg)
![Western Blot: KPNA3 Antibody [NB100-81650] - EZH2-regulated miR-30a targeted KPNB1 in MPNST cells. (A) Putative miR-30a & miR-30d target site in the wild-type KPNB1 3’UTR region. (B) qRT-PCR analyses of miR-30a in MPNST724 & S462 cells transfected with a negative control or EZH2 siRNA. miR-30a expression was normalized to SNORD47. Data are shown as mean ± SD (n = 3); *p < 0.05, Student t test. (C) qRT-PCR analyses of miR-30a in S462 & MPNST724 cells treated with DZNep for 96 hours. miR-30d expression was normalized to SNORD47. Mean ± SD values are shown (n = 3); *p < 0.05, Student t test. (D) Western blot analyses of KPNB1 & actin in S462 & MPNST724 cells transfected with a negative control (Ctl) or miR-30a (30a) mimics. (E) Luciferase reporter assay showed that miR-30a inhibited wild-type (WT) KPNB1 3’UTR reporter activity but not mutant (MT) KPNB1 3’UTR reporter activity in S462 cells. Mean ± SD values are shown (n = 3); *p < 0.05, Student t test. (F) qRT-PCR analyses of miR-30a expression in normal Schwann cells (NSC) & multiple MPNST cell lines (MPNST724, S462, STS26T, MPNST624, T265, & ST88-14). Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/s12943-015-0325-1), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-81650_rabbit-polyclonal-kpna3-antibody-310202416284927.jpg)