BST2 Antibody (4F6) - BSA Free

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Western Blot: BST2 Antibody (4F6) [NBP2-29622] - WB analysis of BST2 protein in (A) human heart lysate (B) human ovary lysate and on (C) partial recombinant BST2 protein with monoclonal BST2 antibody (clone 4F6) at a ...read more
Immunohistochemistry-Paraffin: BST2 Antibody (4F6) [NBP2-29622] - Analysis of BST2 protein in a section of human lung cancer (squamous cell carcinoma) using 5 ug/mL concentration of BST2 antibody (clone 4F6). The ...read more
Flow (Cell Surface): BST2 Antibody (4F6) [NBP2-29622] - Flow analysis of Molt4 cells using the BST2 antibody [4F6]. The shaded purple region represents cells alone, green represents the isotype control and pink ...read more
Immunohistochemistry-Paraffin: BST2 Antibody (4F6) [NBP2-29622] - Analysis of BST2 protein in a section of human endometrial carcinoma using 5 ug/mL concentration of BST2 antibody (clone 4F6). The carcinoma cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Flow-CS, IHC, IHC-P, CyTOF-ready
Clone
4F6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

BST2 Antibody (4F6) - BSA Free Summary

Immunogen
Full length recombinant human BST2 protein [NCBI NP_004326].
Localization
Cell membrane, trans-Golgi network, late endosome and cytoplasm
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BST2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Cell Surface)
  • Flow Cytometry 0.5 ug/mL
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Western Blot 1.0 ug/mL
Application Notes
The unprocessed form of BST2 protein is 180 amino acids long (161 amino acids in the cleaved BST) and its cleaved form undergoes further modifications namely disulfide bond formation, GPI-anchor/GPI-like-anchor binding, glycosylation, isopeptide bond formation, lipidation and Ubl conjugation. The predicted molecular weight of unmodified BST2 protein is ~17-18 kDa, whereas the post-translationally modified protein has been documented to run at ~25-36 kDa (glycosylated), 50-55 kDa (ubiquitinated) and 65-70 kDa (dimers). BST2 protein localizes mainly to the cell membranes from where it shuttles to the trans-Golgi network, late endosome and the cytoplasm. In immuno-staining assays, BST2 may develop a membrane-cytoplasmic staining pattern. This antibody is CyTOF ready.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for BST2 Antibody (4F6) - BSA Free

  • bone marrow stromal antigen 2
  • bone marrow stromal cell antigen 2
  • BST2
  • BST-2
  • CD317 antigen
  • CD317
  • HM1.24 Antigen
  • NPC-A-7
  • PDCA-1
  • Tetherin

Background

BST2 was first identified as a novel terminal B-cell-restricted antigen termed HM1.24 that is expressed on certain bone marrow cells as well as other tissues. The surface expression of Bst2 on fibroblast cell lines facilitated the stromal cell-dependent growth of DW34, a pre-B-cell line, suggesting that this protein is involved in pre-B-cell growth. Later experiments also showed it to be upregulated in several myeloma cells. More recently, Bst2 was identified an inhibitor of retroviral release from human cells whose activity is antagonized by the HIV-1 accessory protein Vpu. While Bst2 caused retention of virions on cell surfaces or endocytosis into Bst2-positive compartments, its depletion abolished the viral requirement for Vpu for virus release. This activity may represent a potential therapeutic strategy for the treatment of HIV/AIDS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BST2
Entrez