| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56437SS | |||
| BSA Free | NB100-56437 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-149SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-75478SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81880-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85067-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-65956-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72984 | |||
![Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Western blot shows lysates of Jurkat human T lymphocyte parental cell line and CXCR4 knockout (KO) Jurkat cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CXCR4 Polyclonal Antibody (Catalog # NB100-56437) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CXCR4 at approximately 38 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in the knockout Jurkat cell line. This experiment was conducted under reducing conditions. Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Western-Blot-NBP2-24862-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Western blot shows lysates of Jurkat human T lymphocyte parental cell line and CXCR4 knockout (KO) Jurkat cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CXCR4 Polyclonal Antibody (Catalog # NB100-56437) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CXCR4 at approximately 38 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in the knockout Jurkat cell line. This experiment was conducted under reducing conditions. Image using the standard format of this product.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CXCR4 Antibody - Azide Free [NBP2-24862] - Jurkat cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-CXCR4 (NB100-56437) at a 1:25 dilution for 1 hour at room temperature and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-24862-img0002.jpg)
![Immunohistochemistry: CXCR4 Antibody - Azide Free [NBP2-24862] - CXCR4 was detected in immersion sections of human tonsil using rabbit anti-human antibody (Catalog # NB100-56437) at 1:300 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-rabbit HRP polymer detection reagent with DAB chromogen (brown) and counterstained with hematoxylin (blue). Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Immunohistochemistry-NBP2-24862-img0003.jpg)
![Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Analysis of 15 ug of whole cell lysate from HeLa cells with anti-CXCR4 at 1 ug/ml.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Western-Blot-NBP2-24862-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MDH2 Antibody [NBP1-32259] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MDH2 stained by MDH2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10um.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32259-img0023.jpg "Immunocytochemistry/Immunofluorescence: MDH2 Antibody [NBP1-32259] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MDH2 stained by MDH2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10um.")
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Rat colon. MDH2 stained by MDH2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0021.jpg)
![Western Blot: MDH2 Antibody [NBP1-32259] - Analysis of MDH2 in HeLa whole cell lysate. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-32259-img0007.jpg)
![Western Blot: MDH2 Antibody [NBP1-32259] - Sample (20 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-32259-img0009.jpg)
![Western Blot: MDH2 Antibody [NBP1-32259] - A. 50 ug rat lung lysate/extract 10 % SDS-PAGEMDH2 antibody dilution: 1:5000](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-32259-img0012.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - H1299 xenograft. MDH2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0006.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Mouse heart. MDH2 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0013.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Mouse heart. MDH2 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0014.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Rat liver. MDH2 stained by MDH2 antibody diluted at 1:1000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0018.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Rat testis. MDH2 stained by MDH2 antibody diluted at 1:1000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0019.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Mouse testis. MDH2 stained by MDH2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0020.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MDH2 Antibody [NBP1-32259] - Wild-type (WT) and MDH2 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-32259-img0022.jpg)
![Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-Western-Blot-NB100-240-img0016.jpg "Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: TOR/mTOR Antibody [NB100-240] - Detection of human mTOR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-mTOR antibody NB100-240 used for IP at 3 ug per reaction. mTOR was also immunoprecipitated by rabbit anti-mTOR antibody NB100-241. For blotting immunoprecipitated mTOR, NB100-240 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-Immunoprecipitation-NB100-240-img0015.jpg)
![Western Blot: PDZK1 Antibody [NB400-149] - WB analysis of PDZK1 in fetal human liver lysate.](http://images.novusbio.com/fullsize/PDZK1-Antibody-Western-Blot-NB400-149-img0003.jpg "Western Blot: PDZK1 Antibody [NB400-149] - WB analysis of PDZK1 in fetal human liver lysate.")
![Immunocytochemistry/Immunofluorescence: PDZK1 Antibody [NB400-149] - PDZK1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PDZK1-Antibody-Immunocytochemistry-Immunofluorescence-NB400-149-img0004.jpg)
![Immunohistochemistry: PDZK1 Antibody [NB400-149] - IHC analysis of PDZK1 in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/PDZK1-Antibody-Immunohistochemistry-NB400-149-img0005.jpg)
![Simple Western: PDZK1 Antibody [NB400-149] - Simple Western lane view shows a specific band for PDZK1 in 0.2 mg/ml of Human Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.](http://images.novusbio.com/fullsize/PDZK1-Antibody-Simple-Western-NB400-149-img0006.jpg)
![Western Blot: Dicer Antibody [NBP1-06520] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiDicer antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Dicer-Antibody-Western-Blot-NBP1-06520-img0003.jpg "Western Blot: Dicer Antibody [NBP1-06520] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiDicer antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation: Dicer Antibody [NBP1-06520] - Detection of human Dicer by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Dicer antibody NBP1-06520 (lot NBP1-06520-5) used for IP at 6 ug per reaction. Dicer was also immunoprecipitated by a previous lot of this antibody (lot NBP1-06520-4). For blotting immunoprecipitated Dicer, NBP1-06520 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Dicer-Antibody-Immunoprecipitation-NBP1-06520-img0004.jpg)
![Western Blot: Dicer Antibody [NBP1-06520] - Nef does not affect siRNA loading into RISC, but inhibits its slicing activity.(A) Lysates from the U937 stable cell lines were prepared in Buffer D as described in Materials & Methods. From this, 25 µg of lysate was incubated with 32P-labeled duplex siRNA (without or with 50-fold molar excess of unlabeled siRNA) in binding buffer for 30 min at 4°C. The complexes were separated on a 6% non-denaturing polyacrylamide gel. (B) The gel shift assay was set up as in (A) except that lysates immunodepleted for either Ago2 or Dicer were also included. The positions of the mobility shifted complexes & free probe are indicated. (C) Slicer activity was assayed using immunoprecipitated Ago2 from U937/Nef-EYFP or U937/EYFP cell lysates & a 32P-labeled in vitro transcribed let7a RNA, as described in Materials & Methods. The positions of full-length RNA & the two sliced products are shown. The 59 nt marker oligonucleotide was run on the same gel, but a lower exposure is shown. (D) Densitometric analysis of autoradiograms from three independent experiments was carried out using Image J (version 1.4.1). The slicing activity in Nef-EYFP lysates is represented as a percentage of that in EYFP lysates. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0074472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06520_rabbit-polyclonal-dicer-antibody-310202416175285.jpg)
![Western Blot: ATF6 Antibody [NBP1-75478] - Total protein from HeLa cells treated with and without 1 mM DTT for 24 hours was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 1% BSA. The membrane was probed with 2.0 ug/ml anti-ATF6 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the decrease in full length ATF6 (90 kDa, arrow) and increase in cleaved ATF6 (55 kDa, arrowhead) upon DTT treatment and the activation of the unfolded protein response pathway.](http://images.novusbio.com/fullsize/ATF6-Antibody-Western-Blot-NBP1-75478-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATF6 Antibody [NBP1-75478] - Total protein from HeLa cells treated with and without 1 mM DTT for 24 hours was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 1% BSA. The membrane was probed with 2.0 ug/ml anti-ATF6 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the decrease in full length ATF6 (90 kDa, arrow) and increase in cleaved ATF6 (55 kDa, arrowhead) upon DTT treatment and the activation of the unfolded protein response pathway.")
![<b>Biological Strategies Validation. </b>Simple Western: ATF6 Antibody [NBP1-75478] - Simple Western lane view shows a specific band for ATF6 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/ATF6-Antibody-Simple-Western-NBP1-75478-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP1-75478] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-ATF6 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-75478-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ATF6 Antibody - BSA Free [NBP1-75478] - AICAR inhibits ER stress responses. Western analysis of cell lysates of nuclear extracts. AMPK-independent inhibition of human macrophage ER stress response by AICAR. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27562249/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ATF6-Antibody-Western-Blot-NBP1-75478-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP1-75478] - Antibody was tested in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).](http://images.novusbio.com/fullsize/ATF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-75478-img0002.jpg)
![Western Blot: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 expression in U-2 OS (Cat # L022V1).](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Western-Blot-H00022943-M11-img0012.jpg "Western Blot: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 expression in U-2 OS (Cat # L022V1).")
![Immunohistochemistry-Paraffin: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of monoclonal antibody to DKK1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Immunohistochemistry-Paraffin-H00022943-M11-img0009.jpg)
![Immunoprecipitation: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 transfected lysate using anti-DKK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with DKK1 rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Immunoprecipitation-H00022943-M11-img0010.jpg)
![ELISA: Dkk-1 Antibody (2A5) [H00022943-M11] - Detection limit for recombinant GST tagged DKK1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-ELISA-H00022943-M11-img0013.jpg)
![Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 activity is involved in serum-induced or LPA-induced YAP/TAZ dephosphorylation and activation. FBS and LPA activate YAP/TAZ and induce NUAK2 expression. YAP/TAZ phosphorylation status was monitored using Phos-Tag gels, while NUAK2 and ANKRD1 mRNA expression was determined by qPCR at the indicated times. Expression data are plotted as the mean +/- SD (n = 3). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05939-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Western-Blot-NBP1-81880-img0017.jpg "<b>Biological Strategies Validation. </b>Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 activity is involved in serum-induced or LPA-induced YAP/TAZ dephosphorylation and activation. FBS and LPA activate YAP/TAZ and induce NUAK2 expression. YAP/TAZ phosphorylation status was monitored using Phos-Tag gels, while NUAK2 and ANKRD1 mRNA expression was determined by qPCR at the indicated times. Expression data are plotted as the mean +/- SD (n = 3). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05939-2), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NUAK2 Antibody [NBP1-81880] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli fibrillar center & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81880-img0010.jpg)
![Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880] - Staining of human testis shows moderate to strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Immunohistochemistry-Paraffin-NBP1-81880-img0015.jpg)
![Western Blot: NUAK2 Antibody [NBP1-81880] - Analysis in control (vector only transfected HEK293T lysate) and NUAK2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/NUAK2-Antibody-Western-Blot-NBP1-81880-img0011.jpg)
![Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880] - Staining of human cerebellum shows strong membranous positivity in Purkinje cells.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Immunohistochemistry-Paraffin-NBP1-81880-img0012.jpg)
![Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880] - Staining of human cervix shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Immunohistochemistry-Paraffin-NBP1-81880-img0013.jpg)
![Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880] - Staining of human duodenum shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Immunohistochemistry-Paraffin-NBP1-81880-img0014.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 regulates YAP/TAZ activity through LATS. NUAK2 requires LATS to regulate YAP/TAZ localization, phosphorylation, and target gene expression in MDA-MB231 cells. YAP/TAZ phosphorylation was monitored using Phos-Tag gels. Relative phosphorylation levels from blots is quantitated (right). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05939-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Knockdown-Validated-NBP1-81880-img0016.jpg)
![Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 activity is involved in serum-induced or LPA-induced YAP/TAZ dephosphorylation & activation. a, b FBS & LPA activate YAP/TAZ & induce NUAK2 expression. YAP/TAZ phosphorylation status was monitored using Phos-Tag gels (a), while NUAK2 & ANKRD1 mRNA expression was determined by qPCR at the indicated times. Expression data are plotted as the mean ± SD (n = 3). c–h WZ4003 (10 μM) blocks serum/LPA-induced NUAK2 & ANKRD1 mRNA expression. FBS-induced or LPA-induced YAP/TAZ dephosphorylation (e & g), or NUAK2 & ANKRD1 mRNA expression (c, f, h) was monitored at the indicated times (d). Expression data are plotted as the mean ± range for a representative experiment (c, f, h) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30158528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81880_rabbit-polyclonal-nuak2-antibody-31020241535685.jpg)
![Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 regulates YAP/TAZ activity through LATS. a Loss of NUAK2 enhances YAP/TAZ phosphorylation in MDA-MB231 cells. Quantitation of relative phosphorylation levels from blots is shown. b NUAK2 interacts with LATS1 & LATS2, but not MST1, MST2, or TAZ in the LUMIER protein interaction screen. c Lysates from HEK293T cells, transfected with NUAK2-Flag, were subjected to immunoprecipitation (IP) using anti-LATS1 antibody for endogenous LATS1 & co-immunoprecipitated NUAK2-Flag was detected by immunoblotting. Protein expression levels were confirmed (Totals). WT wild type, KR K81R, kinase dead. d–f NUAK2 requires LATS to regulate YAP/TAZ localization, phosphorylation, & target gene expression in MDA-MB231 cells. d YAP/TAZ localization was quantitated & plotted as the mean ± SD (n = 3) with representative images shown on the right. Scale bars, 25 μm. N: nuclear, C: cytoplasmic. e Target gene expression was determined by qPCR. Data are plotted as the mean ± SD (n = 3). f YAP/TAZ phosphorylation was monitored using Phos-Tag gels. Relative phosphorylation levels from blots is quantitated (right) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30158528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81880_rabbit-polyclonal-nuak2-antibody-31020241612198.jpg)
![Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 regulates YAP/TAZ activity through LATS. a Loss of NUAK2 enhances YAP/TAZ phosphorylation in MDA-MB231 cells. Quantitation of relative phosphorylation levels from blots is shown. b NUAK2 interacts with LATS1 & LATS2, but not MST1, MST2, or TAZ in the LUMIER protein interaction screen. c Lysates from HEK293T cells, transfected with NUAK2-Flag, were subjected to immunoprecipitation (IP) using anti-LATS1 antibody for endogenous LATS1 & co-immunoprecipitated NUAK2-Flag was detected by immunoblotting. Protein expression levels were confirmed (Totals). WT wild type, KR K81R, kinase dead. d–f NUAK2 requires LATS to regulate YAP/TAZ localization, phosphorylation, & target gene expression in MDA-MB231 cells. d YAP/TAZ localization was quantitated & plotted as the mean ± SD (n = 3) with representative images shown on the right. Scale bars, 25 μm. N: nuclear, C: cytoplasmic. e Target gene expression was determined by qPCR. Data are plotted as the mean ± SD (n = 3). f YAP/TAZ phosphorylation was monitored using Phos-Tag gels. Relative phosphorylation levels from blots is quantitated (right) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30158528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81880_rabbit-polyclonal-nuak2-antibody-310202416113541.jpg)
![Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 is associated with high-grade human bladder cancer. a, c Expression of NUAK2 & the YAP Signature Genes (YAP SG) is enhanced both in high-grade non-muscle-invasive (HG-NMIBC; n = 13, **p = 0.0071) & muscle-invasive (pT2 MIBC; n = 9, **p = 0.0033) bladder cancers39 as compared to LG-NMIBC (n = 27) using an unpaired t test. b Elevated expression of NUAK2 is correlated with disease recurrence in a cohort41 of MIBC patient samples with n = 57 disease free & n = 56, recurred samples (*p = 0.0172). Box & whisker plots show the median (line in the box), first & third quartiles (lower & upper ends of the box), & the minimum & maximum values (whiskers in the plot). Dots represent a single patient sample. d A heat map depicting expression of NUAK2 & YAP SG in NMIBC LG (open circle), HG (closed circle), & MIBC pT2 (slashed circle) bladder cancer samples39. e Characterization of high-grade-derived & low-grade-derived bladder cancer cell lines. Scale bar, 25 μm. f NUAK2 mRNA expression is elevated in HG-derived (TCCSUP & T24) BC cell lines as compared to LG lines. Data are plotted as the mean ± SD (n = 3). g–i Blocking NUAK2 activity with 10 μM WZ4003 (g) or NUAK2 expression in stable clones (#5 or #7) using inducible NUAK2 shRNAs (h, i) inhibits the growth of HG BC cell lines as measured by SRB (g) or DAPI (h) staining. Data are plotted as the mean ± SD of a representative experiment, n = 3 (for TCC) or mean ± range of two experiments (for T24) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30158528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81880_rabbit-polyclonal-nuak2-antibody-310202416114727.jpg)
![Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 regulates YAP/TAZ activity through LATS. a Loss of NUAK2 enhances YAP/TAZ phosphorylation in MDA-MB231 cells. Quantitation of relative phosphorylation levels from blots is shown. b NUAK2 interacts with LATS1 & LATS2, but not MST1, MST2, or TAZ in the LUMIER protein interaction screen. c Lysates from HEK293T cells, transfected with NUAK2-Flag, were subjected to immunoprecipitation (IP) using anti-LATS1 antibody for endogenous LATS1 & co-immunoprecipitated NUAK2-Flag was detected by immunoblotting. Protein expression levels were confirmed (Totals). WT wild type, KR K81R, kinase dead. d–f NUAK2 requires LATS to regulate YAP/TAZ localization, phosphorylation, & target gene expression in MDA-MB231 cells. d YAP/TAZ localization was quantitated & plotted as the mean ± SD (n = 3) with representative images shown on the right. Scale bars, 25 μm. N: nuclear, C: cytoplasmic. e Target gene expression was determined by qPCR. Data are plotted as the mean ± SD (n = 3). f YAP/TAZ phosphorylation was monitored using Phos-Tag gels. Relative phosphorylation levels from blots is quantitated (right) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30158528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81880_rabbit-polyclonal-nuak2-antibody-31020241612229.jpg)
![Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NBP1-85067] - Staining in human placenta and skeletal muscle tissues . Corresponding WWTR1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Immunohistochemistry-Paraffin-NBP1-85067-img0029.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NBP1-85067] - Staining in human placenta and skeletal muscle tissues . Corresponding WWTR1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: TAZ/WWTR1 Antibody [NBP1-85067] - Analysis in EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Western-Blot-NBP1-85067-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: TAZ/WWTR1 Antibody [NBP1-85067] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear bodies and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85067-img0030.jpg)
![Western Blot: TAZ/WWTR1 Antibody [NBP1-85067] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Western-Blot-NBP1-85067-img0015.jpg)
![Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NBP1-85067] - Staining of human colon shows moderate to strong nuclear positivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Immunohistochemistry-Paraffin-NBP1-85067-img0024.jpg)
![Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NBP1-85067] - Staining of human kidney shows moderate nuclear positivity in glomerular cells and a subset of cells in distal tubules.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Immunohistochemistry-Paraffin-NBP1-85067-img0025.jpg)
![Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NBP1-85067] - Staining of human placenta shows moderate to strong nuclear positivity in endothelial cells.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Immunohistochemistry-Paraffin-NBP1-85067-img0026.jpg)
![Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NBP1-85067] - Staining of human renal cancer shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Immunohistochemistry-Paraffin-NBP1-85067-img0027.jpg)
![Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody [NBP1-85067] - Staining of human skeletal muscle shows weak positivity in myocytes.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Immunohistochemistry-Paraffin-NBP1-85067-img0028.jpg)
![Simple Western: TAZ/WWTR1 Antibody [NBP1-85067] - Simple Western lane view shows a specific band for WWTR1 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Simple-Western-NBP1-85067-img0007.jpg)
![Simple Western: TAZ/WWTR1 Antibody [NBP1-85067] - Electropherogram image(s) of corresponding Simple Western lane view. TAZ/WWTR1 antibody was used at 1:30 dilution on RT-4 lysates(s).](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Simple-Western-NBP1-85067-img0009.jpg)
![Immunohistochemistry-Paraffin: NKG2D/CD314 Antibody (1D11) - Low Endotoxin [NB100-65956] - Analysis of human tonsil tissue using NKG2D/CD314 antibody. PFA fixed paraffin-embedded sections. Heat-mediated antigen retrieval was performed for 30 minutes in citrate buffer. Primary antibody dilution was 1:200. Image from verified customer review.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Low-Endotoxin-Immunohistochemistry-Paraffin-NB100-65956-img0013.jpg "Immunohistochemistry-Paraffin: NKG2D/CD314 Antibody (1D11) - Low Endotoxin [NB100-65956] - Analysis of human tonsil tissue using NKG2D/CD314 antibody. PFA fixed paraffin-embedded sections. Heat-mediated antigen retrieval was performed for 30 minutes in citrate buffer. Primary antibody dilution was 1:200. Image from verified customer review.")
![Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Analysis using the Biotin conjugate of NB100-65956. Staining of Human peripheral blood lymphocytes with biotinylated 1D11, followed by Sav-PE.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Flow-Cytometry-NB100-65956-img0008.jpg)
![Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody,](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Immunohistochemistry-Frozen-NB100-65956-img0009.jpg)
![Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody,](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Immunohistochemistry-Frozen-NB100-65956-img0010.jpg)
![Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Immunohistochemistry-Frozen-NB100-65956-img0011.jpg)
![Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Immunohistochemistry-Frozen-NB100-65956-img0012.jpg)
![Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD314:Low Endotoxin.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Flow-Cytometry-NB100-65956-img0004.jpg)
![Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Human peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Flow-Cytometry-NB100-65956-img0005.jpg)
![Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Human peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-1D11-Flow-Cytometry-NB100-65956-img0006.jpg)
![Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells and NNMT-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-NNMT monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-OTI3D8-Western-Blot-NBP2-00537-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells and NNMT-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-NNMT monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NNMT Antibody (3D8) [NBP2-00537] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NNMT.](http://images.novusbio.com/fullsize/NNMT-Antibody-3D8-Immunocytochemistry-Immunofluorescence-NBP2-00537-img0008.jpg)
![Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Human prostate tissue using anti-NNMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NNMT-Antibody-3D8-Immunohistochemistry-Paraffin-NBP2-00537-img0007.jpg)
![Flow Cytometry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537] - Analysis of Jurkat cells, using anti-NNMT antibody (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Nicotinamide-N-Methyltransferase-NNMT-Antibody-OTI3D8-Flow-Cytometry-NBP2-00537-img0010.jpg)
![Western Blot: NNMT Antibody (3D8) [NBP2-00537] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NNMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NNMT.](http://images.novusbio.com/fullsize/NNMT-Antibody-3D8-Western-Blot-NBP2-00537-img0001.jpg)
![Western Blot: NNMT Antibody (3D8) [NBP2-00537] Analysis of extracts (35ug) from 9 different cell lines by using anti-NNMT monoclonal antibody.](http://images.novusbio.com/fullsize/NNMT-Antibody-3D8-Western-Blot-NBP2-00537-img0009.jpg)
![Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NNMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NNMT-Antibody-3D8-Immunohistochemistry-Paraffin-NBP2-00537-img0002.jpg)
![Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NNMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NNMT-Antibody-3D8-Immunohistochemistry-Paraffin-NBP2-00537-img0003.jpg)
![Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Human endometrium tissue using anti-NNMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NNMT-Antibody-3D8-Immunohistochemistry-Paraffin-NBP2-00537-img0004.jpg)
![Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Human liver tissue using anti-NNMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NNMT-Antibody-3D8-Immunohistochemistry-Paraffin-NBP2-00537-img0005.jpg)
![Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Human pancreas tissue using anti-NNMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NNMT-Antibody-3D8-Immunohistochemistry-Paraffin-NBP2-00537-img0006.jpg)
![Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8)[NBP2-00537]Simple Western� analysis of endogenous protein CDK2 from HELA lysates (0.2 mg/mL) using CDK2 Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp2-00537_mouse-nicotinamide-n-methyltransferase-nnmt-mab-oti3d8-simple-western-131120251236226.jpg%253fstore=america&image-type=image)