| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61137 | |||
| BSA Free | NB300-146SS | |||
| Azide and BSA Free | NBP2-80956 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-119SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-102SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB300-101SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-201SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92683SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47365-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92178-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84466-25ul | |||
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Transfected SK-N-AS cells express ICAM-2 transcripts and proteins. ICAM-2 WT and variants localized to cell membranes. Experimental details are included in Methods. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-261), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Western-Blot-NB300-146-img0022.jpg "Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Transfected SK-N-AS cells express ICAM-2 transcripts and proteins. ICAM-2 WT and variants localized to cell membranes. Experimental details are included in Methods. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-261), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - The cellular model of the blood-CSF barrier. Immunofluorescent staining of Na+K+ ATPase and ABCC1, showing the expected respective apical and basolateral membrane localization in the choroidal epithelial cells. The left image is a close up of a single cell to better appreciate the polarity of distribution of the 2 proteins. Nuclei appear in blue. Arrows show the lateral cellular membranes best seen in the z direction by confocal analysis, arrowheads show the basal labeling of ABCC1. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0150945), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunocytochemistry-Immunofluorescence-NB300-146-img0023.jpg)
![Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Staining of human enodmetrial glands within the uterus using NB300-146. Note the absence of staining in the surrounding myometrial smooth muscle.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunohistochemistry-NB300-146-img0012.jpg)
![Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on NIH3T3 cells with Sodium Potassium ATPase Alpha 1 Antibody (464.6) NB300-146 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Flow-Cytometry-NB300-146-img0027.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Analysis detecting Na, K-ATPase (alpha) in porcine proximal tubule protein.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Western-Blot-NB300-146-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Sodium Potassium ATPase Alpha 1 (464.6) at 10 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunocytochemistry-Immunofluorescence-NB300-146-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Sodium Potassium ATPase Alpha 1 (464.6) at 10 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunocytochemistry-Immunofluorescence-NB300-146-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Detection of ATPA1 (Green) in HepG2 cells using NB300-146. Nuclei (Blue) were counterstained using Hoechst 33258.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunocytochemistry-Immunofluorescence-NB300-146-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - The cellular model of the blood-CSF barrier. Immunofluorescent staining of Na+K+ ATPase and ABCC1, showing the expected respective apical and basolateral membrane localization in the choroidal epithelial cells. The left image is a close up of a single cell to better appreciate the polarity of distribution of the 2 proteins. Nuclei appear in blue. Arrows show the lateral cellular membranes best seen in the z direction by confocal analysis, arrowheads show the basal labeling of ABCC1. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0150945), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunocytochemistry-Immunofluorescence-NB300-146-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - ARPE-19 cells cultured on transwell inserts for 4 weeks are not completely polarized. ARPE-19 cells were cultured up to 4 weeks on transwell inserts covered with laminin. The expression of Na+, K+-ATPase using anti-alpha1 antibody was observed mostly at the basolateral membrane. Scale bar: 10 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fphys.2016.00450/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunocytochemistry-Immunofluorescence-NB300-146-img0020.jpg)
![Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on A549 cells with NB300-146 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Flow-Cytometry-NB300-146-img0016.jpg)
![Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on A549 cells with NBP2-61137AF647 (Blue) and a matched isotype control (Orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image using the Alexa Fluor 647 form of this antibody.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Flow-Cytometry-NB300-146-img0017.jpg)
![Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on A549 cells with - Purified NBP2-61137 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems). Image using the Purified form of this antibody.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Flow-Cytometry-NB300-146-img0018.jpg)
![Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on RCC4 cells with Sodium Potassium ATPase Alpha 1 Antibody (464.6) NB300-146 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Flow-Cytometry-NB300-146-img0025.jpg)
![Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on SK-MEL-28 cells with Sodium Potassium ATPase Alpha 1 Antibody (464.6) NB300-146 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Flow-Cytometry-NB300-146-img0026.jpg)
![Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - ARPE-19 cells cultured on transwell inserts for 4 weeks are not completely polarized. ARPE-19 cells were cultured up to 4 weeks on transwell inserts covered with laminin. The expression of Na+, K+-ATPase using anti-alpha1 antibody was observed mostly at the basolateral membrane. Scale bar: 10 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fphys.2016.00450/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-NB300-146-img0024.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - The cellular model of the blood-CSF barrier.(A) Schematic representation of a choroidal villus & of the experimental set up illustrating the two-chamber culture device. Left: the choroidal epithelium which forms the actual tight barrier controlling access into the CSF [18] delimits a stroma, in which the fenestrated vessels lacking a typical blood-brain barrier phenotype, express P-selectin [4]. Right: The epithelial cell monolayer grown on the lower side of the filter separates the upper chamber corresponding to the blood/stromal or basolateral space, from the bottom chamber representing the CSF or apical compartment (adapted from [18,41]. (B & C) Immunofluorescent staining of occludin (B) & claudin 1/3 (C) showing a typical intercellular distribution of the tight junction proteins in the confluent inverted monolayers of choroidal epithelial cells. (D) Immunofluorescent staining of Na+K+ ATPase & ABCC1, showing the expected respective apical & basolateral membrane localization in the choroidal epithelial cells. The left image is a close up of a single cell to better appreciate the polarity of distribution of the 2 proteins. Nuclei appear in blue. Arrows show the lateral cellular membranes best seen in the z direction by confocal analysis, arrowheads show the basal labeling of ABCC1. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0150945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-146_mouse-monoclonal-sodium-potassium-atpase-alpha-1-antibody-464-6-310202416205173.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Endothelial-mesenchymal transformation (EMT) of RCECs using specific endothelial-mesenchymal transformation medium (SEMTM). (A) Immunohistochemistry of the fibroblastic markers alpha -SMA & vimentin were characteristic of fibroblasts in EMT-RCECs compared to the hexogonal morphology of Fresh RCECs (Scale bar upper panel = 100 µm, Scale bar middle & bottom panel = 50 µm). (B) RT-PCR showed that both Fresh RCECs & EMT-RCECs expressed alpha -Sma. Original gel is shown in Supplemental Fig. S2. However, qRT-PCR in the lower panel shows alpha -Sma was significantly upregulated in EMT-RCECs. (C) Western blots showed that Fresh RCECs & EMT-RCECs expressed Vimentin, Atp1a1, a marker of differentiated endothelial cells, & b-actin. Original gel & blots are shown in Supplemental Figs S3, 4. Semiquantitative analysis shows significant upregulation of the protein levels of Vimentin (p = 0.048) & downregulation of the protein level of Atp1a1 (p = 0.0043). Data expressed as mean ± SD of three replicate experiments. Student’s t test (*p < 0.05, **p < 0.01). (n = 3). RCECs: rabbit corneal endothelial cells, alpha -SMA: smooth muscle alpha -actin. Atp1a1: Na,K‐ATPase alpha ‐subunit. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30442918), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-146_mouse-monoclonal-sodium-potassium-atpase-alpha-1-antibody-464-6-310202416205123.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - SDS–PAGE western blot validation of the increased abundance of Annexin V, Na/K ATPase & alpha -tubulin in the EVs derived from TIS Cal51 cells compared with EVs derived from non-senescent non-treated controls. EVs isolated from TIS & control cells were immunoblotted to validate the protein expression of annexin V, Na/K ATPase & alpha -tubulin from the MS data. CD63 & HSP70 were used as loading controls. Densitometry was completed using Image J software (Fiji). All values are expressed as the mean of three independent experiments±s.e.m. The limit of ±1 skewness was set as normal distribution. Skewed data were transformed to Y=Log(Y) to fit a normal distribution. Results were analysed using the Student’s t-test with significant differences having a *P⩽0.05, **P⩽0.01, ***P⩽0.001. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/oncsis201782), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-146_mouse-monoclonal-sodium-potassium-atpase-alpha-1-antibody-464-6-310202416203713.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - ARPE-19 cells cultured on transwell inserts for 4 weeks are not completely polarized. ARPE-19 cells were cultured up to 4 weeks on transwell inserts covered with laminin. The immunofluorescence image in (A) shows actin localization using rhodamine phalloidin. The cells are flat with stress fibers & very little circumferential actin microfilament bundles. The expression of CD147, a RPE marker, was detected using a specific antibody in the apical membrane domain (B). The expression of Na+, K+-ATPase using anti-alpha 1(C) & anti-beta 1 antibodies (D) was observed mostly at the basolateral membrane. Immunofluorescence detection with anti-human beta 2 antibody revealed a very weak signal (E). Scale bar: 10 μm. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fphys.2016.00450/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-146_mouse-monoclonal-sodium-potassium-atpase-alpha-1-antibody-464-6-310202416205189.jpg)
![Western Blot: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Analysis of Neurokinin 1 Receptor in 1: human brain lysate, 2: rat brain lysate and 3: monkey brain lysate.](http://images.novusbio.com/fullsize/NK1R-Antibody-Western-Blot-NB300-119-img0009.jpg "Western Blot: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Analysis of Neurokinin 1 Receptor in 1: human brain lysate, 2: rat brain lysate and 3: monkey brain lysate.")
![Immunocytochemistry/Immunofluorescence: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Immunostain of NK1R in rat enteric glial cells. Image from verified customer review.](http://images.novusbio.com/fullsize/NK1R-Antibody-Immunocytochemistry-Immunofluorescence-NB300-119-img0004.jpg)
![Immunohistochemistry: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Staining of human brain, basal nucleus of Meynert, neurons.](http://images.novusbio.com/fullsize/NK1R-Antibody-Immunohistochemistry-NB300-119-img0006.jpg)
![Western Blot: NK1R Antibody [NB300-119] - Total protein from human, mouse and rat brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-NK1R in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/NK1R-Antibody-Western-Blot-NB300-119-img0010.jpg)
![Western Blot: NK1R Antibody [NB300-119] - TACR1 and its downstream targets SRC and p-SRC are expressed in neuroblastoma cell lines. Endogenous TACR1 (NK1R), SRC and p-SRC expression in whole-cell extracts of neuroblastoma cell lines was visualized by western blotting. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.13440), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NK1R-Antibody-Western-Blot-NB300-119-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: NK1R Antibody [NB300-119] - Overnight incubation at 4C with NK1R antibody diluted 1:100 in PBS/BSA 1%. Red: Phalloidin, Blue: DAPI, Green: NK1R antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nb300-119_rabbit-polyclonal-nk1r-antibody-immunocytochemistry-immunofluorescence-1372023145313..jpg)
![Western Blot: NK1R Antibody - BSA Free [NB300-119] - TACR1 & its downstream targets SRC & p-SRC are expressed in neuroblastoma cell lines(A) Endogenous TACR1, SRC & p-SRC expression in whole-cell extracts of neuroblastoma cell lines was visualized by western blotting. GAPDH was used as a loading control. (B) Quantitative RT-PCR of TACR1 mRNA in a panel of neuroblastoma cell lines (n = 3, error bars indicate standard deviation). (C–E) Quantification of SRC (C), p-SRC (D) & TACR1 (E) protein expression using densitometry analysis of western immunoblots (n = 3, error bars indicate standard deviation). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.13440), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-119_rabbit-polyclonal-nk1r-antibody-310202415392524.jpg)
![Western Blot: NK1R Antibody - BSA Free [NB300-119] - Striatal beta 3 subunit protein reduced conditional KO mice. (A) Fluorescent images demonstrating colocalization between endogenous tdTomato fluorescence & DARPP-32 expression. Approximately half of the DARPP-32+ cells expressed endogenous tdTomato, indicative of Cre expression. Scale bar is 25 μm. (B) Representative western blot analysis of the GABAA receptor beta 3 subunit from individual 30-day-old mice revealed reduced amounts of beta 3 subunit protein in striatum (B) from Cre positive ( beta 3f/fDrd2) animals compared to Cre negative ( beta 3f/+ & beta 3f/f) control animals. The amount of beta 3 protein in cortex (C) did not differ between genotypes. Blots were reprobed for beta -actin. (D) Summary graph of western blot analysis demonstrating a significant reduction in beta 3 protein in striatum, but not cortex. Data are expressed as percent change in band intensity relative to Cre negative controls following normalization to actin. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21847370), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-119_rabbit-polyclonal-nk1r-antibody-310202416205147.jpg)
![Western Blot: NK1R Antibody - BSA Free [NB300-119] - Striatal beta 3 subunit protein reduced conditional KO mice. (A) Fluorescent images demonstrating colocalization between endogenous tdTomato fluorescence & DARPP-32 expression. Approximately half of the DARPP-32+ cells expressed endogenous tdTomato, indicative of Cre expression. Scale bar is 25 μm. (B) Representative western blot analysis of the GABAA receptor beta 3 subunit from individual 30-day-old mice revealed reduced amounts of beta 3 subunit protein in striatum (B) from Cre positive ( beta 3f/fDrd2) animals compared to Cre negative ( beta 3f/+ & beta 3f/f) control animals. The amount of beta 3 protein in cortex (C) did not differ between genotypes. Blots were reprobed for beta -actin. (D) Summary graph of western blot analysis demonstrating a significant reduction in beta 3 protein in striatum, but not cortex. Data are expressed as percent change in band intensity relative to Cre negative controls following normalization to actin. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21847370), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-119_rabbit-polyclonal-nk1r-antibody-310202416212385.jpg)
![Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-101] - IF analysis of Neurokinin 1 Receptor in rat brain tissue. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/Neurokinin-1-Receptor-Antibody-Immunocytochemistry-Immunofluorescence-NB300-101-img0007.jpg "Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-101] - IF analysis of Neurokinin 1 Receptor in rat brain tissue. Image courtesy of anonymous customer product review.")
![Immunohistochemistry: Neurokinin 1 Receptor Antibody [NB300-101] - Immunohistochemical analysis of Neurokinin 1 Receptor in mouse brain (selected cells, axon & dendrites) using NB300-101.](http://images.novusbio.com/fullsize/Neurokinin-1-Receptor-Antibody-Immunohistochemistry-NB300-101-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-101] - Confocal image of SPR reactivity using polyclonal anti-NK-1 Receptor (NB 300-101) in a lamina III neuron in the spinal cord of a rat.](http://images.novusbio.com/fullsize/Neurokinin-1-Receptor-Antibody-Immunocytochemistry-Immunofluorescence-NB300-101-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-101] - IF analysis of Neurokinin 1 Receptor in rat brain tissue. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/Neurokinin-1-Receptor-Antibody-Immunocytochemistry-Immunofluorescence-NB300-101-img0010.jpg)
![Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201] - Immunofluorescence photomicrograph showing that the expression of Neurokinin B in the arcuate nucleus (ARC). Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32921318/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody---BSA-Free-Immunohistochemistry-NB300-201-img0009.jpg "<b>Biological Strategies Validation. </b>Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201] - Immunofluorescence photomicrograph showing that the expression of Neurokinin B in the arcuate nucleus (ARC). Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32921318/) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:200 dilution. The antibody depicted an expected strong cytoplasmic staining in the neuronal cells.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody-Immunohistochemistry-Paraffin-NB300-201-img0005.jpg)
![Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:100 dilution. The antibody showed a specific cytoplasmic staining in the Purkinje cells and some granular neurons from the granular layer with weak signal in the fibers within the molecular layer of the section.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody-Immunohistochemistry-Paraffin-NB300-201-img0006.jpg)
![Immunohistochemistry: Neurokinin B Antibody [NB300-201] - IF analysis of Neurokinin B in mouse hypothalamus. Image courtesy of an anonymous customer review.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody-Immunohistochemistry-NB300-201-img0007.jpg)
![Immunohistochemistry: Neurokinin B Antibody [NB300-201] - Detection of Neurokinin B in rat brain tissue (Alexa 488 stain), using NB 300-201.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody-Immunohistochemistry-NB300-201-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: Neurokinin B Antibody - BSA Free [NB300-201] - Troxerutin reduced the expression of Kisspepetin1 & Neurokinin B in the ARC. a Immunofluorescence photomicrograph showing that the expression of Kisspeptin1 & NKB in the arcuate nucleus (ARC). Scale bars:20 μm. b A lower magnification image showing the whole ARC & median eminence. Rectangular frame a & b represent ARC & median eminence (ME) analyzed in Fig. 5a & Fig. 6a, respectively. Scale bars:200 μm. c Quantitative analysis of integral optical density (IOD) of Kisspeptin1 & NKB in the ARC. P values were determined by one-way ANOVA with Tukey’s multiple comparison test & data were presented as means±s.e.m. n = 4 per group. **P < 0.01, ***P < 0.001vs. sham group; #P < 0.05, ##P < 0.01 vs. PCOS group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32921318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-201_rabbit-polyclonal-neurokinin-b-antibody-310202415175279.jpg)
![Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201] - Characterization of Kiss1-null mice.(A-C) Immunofluorescence for kisspeptin (A,B) & NKB (C) in the ARN of wild-type (A) & Kiss1-null (B,C) female mice. (D) Cell-attached recordings showing the effects of 100 nM NKB on firing of KNDy neurons in acute brain slices prepared from female heterozygous Kiss1Cre/+ and homozygous (null) Kiss1Cre/Cre;Rosa26-tdT mice. (E) Mean ± SEM changes in KNDy neuron firing evoked by 100 nM NKB in heterozygous Kiss1Cre/+and homozygous (null) Kiss1Cre/Cre;Rosa26-tdT mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33464205), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-201_rabbit-polyclonal-neurokinin-b-antibody-310202415291957.jpg)
![Western Blot: Adenylate Cyclase 3 Antibody [NBP1-92683] - Analysis of HEK293 cell lysates using Adenylate Cyclase 3 antibody, dilution 1:2000 (Green): [1] protein standard, [2] non-transfected HEK293 cells, and [3] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human Adenylate Cyclase 3 cDNA. The strong band at about 130kDa in the transfected cells demonstrates overexpression of the human Adenylate Cyclase 3 protein, and the bands over 250kDa presumably correspond to either glycosylated or aggregated forms of Adenylate Cyclase 3. The blot was simultaneously probed with mouse GAPDH mAb, dilution 1:5000 (Red), which reveals the single band at ~37kDa in both transfected and non-transfected cells.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Western-Blot-NBP1-92683-img0005.jpg "Western Blot: Adenylate Cyclase 3 Antibody [NBP1-92683] - Analysis of HEK293 cell lysates using Adenylate Cyclase 3 antibody, dilution 1:2000 (Green): [1] protein standard, [2] non-transfected HEK293 cells, and [3] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human Adenylate Cyclase 3 cDNA. The strong band at about 130kDa in the transfected cells demonstrates overexpression of the human Adenylate Cyclase 3 protein, and the bands over 250kDa presumably correspond to either glycosylated or aggregated forms of Adenylate Cyclase 3. The blot was simultaneously probed with mouse GAPDH mAb, dilution 1:5000 (Red), which reveals the single band at ~37kDa in both transfected and non-transfected cells.")
![Immunocytochemistry/Immunofluorescence: Adenylate Cyclase 3 Antibody [NBP1-92683] - Analysis of rat hippocampus stained with NBP1-92683 antibody to adenylate cyclase III in red and mouse antibody to MECP2 in green, DNA is revealed with DAPI in blue. This is a stacked confocal image made with a 100X objective lens. The ACIII antibody reveals neuronal cilia while the MECP2 antibody reveals the nuclei of certain neurons to a variable degree.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92683-img0004.jpg)
![Western Blot: Adenylate Cyclase 3 Antibody [NBP1-92683] - Western blot of rat olfactory epithelium (OE) and frontal cortex (FC). NBP1-92683 stains a band at about 200kDa in olfactory epithelium which is rich in cilia. Fewer cilia are found in frontal cortex, and the protein is less heavily glycosylated, and a less prominent band is seen at about 160kDa. Data generated in the laboratory of Matt Sarkisian in the University of Florida.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Western-Blot-NBP1-92683-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Adenylate Cyclase 3 Antibody [NBP1-92683] - Mixed neuron-glial cultures stained with Adenylate Cyclase 3 antibody (NBP1-92683, red) and Alpha Fodrin antibody (NBP1-92689, green). Note the strong and clean staining of neuronal cilia. Since Alpha Fodrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92683-img0003.jpg)
![Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of GAPDHS expression in transfected 293T cell line by GAPDS monoclonal antibody (M01), clone 2E3-2E10.Lane 1: GAPDHS transfected lysate(44.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-2E3-2E10-Western-Blot-H00026330-M01-img0009.jpg "Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of GAPDHS expression in transfected 293T cell line by GAPDS monoclonal antibody (M01), clone 2E3-2E10.Lane 1: GAPDHS transfected lysate(44.5 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of monoclonal antibody to GAPDHS on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-2E3-2E10-Immunohistochemistry-Paraffin-H00026330-M01-img0007.jpg)
![Sandwich ELISA: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Detection limit for recombinant GST tagged GAPDHS is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-2E3-2E10-Sandwich-ELISA-H00026330-M01-img0008.jpg)
![Western Blot: MT-ND4 Antibody [NBP2-47365] - Analysis in human cell line HeLa.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Western-Blot-NBP2-47365-img0003.jpg "Western Blot: MT-ND4 Antibody [NBP2-47365] - Analysis in human cell line HeLa.")
![Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-47365] - Staining of human Stomach shows strong granular cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Immunohistochemistry-Paraffin-NBP2-47365-img0011.jpg)
![Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-47365] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Immunohistochemistry-Paraffin-NBP2-47365-img0004.jpg)
![Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-47365] - Staining of human Lymph node shows very weak granular cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Immunohistochemistry-Paraffin-NBP2-47365-img0009.jpg)
![Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-47365] - Staining of human Salivary gland shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Immunohistochemistry-Paraffin-NBP2-47365-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (3L6F0) [NBP3-15427] - Immunofluorescence analysis of NIH-3T3 cells using Sodium Potassium ATPase Alpha 1 Rabbit mAb (NBP3-15427) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-3L6F0-Immunocytochemistry-Immunofluorescence-NBP3-15427-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (3L6F0) [NBP3-15427] - Immunofluorescence analysis of C6 cells using Sodium Potassium ATPase Alpha 1 Rabbit mAb (NBP3-15427) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-3L6F0-Immunocytochemistry-Immunofluorescence-NBP3-15427-img0004.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (3L6F0) [NBP3-15427] - Analysis of extracts from HeLa cells, using Na+/K+-ATPase Rabbit mAb at 1:50000 dilution.Membrane protein extract isolated from Hela cells. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15427_rabbit-sodium-potassium-atpase-alpha-1-mab-3l6f0-western-blot-20620239331..jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (3L6F0) [NBP3-15427] - Analysis of various lysates, using Na+/K+-ATPase Rabbit mAb at 1:50000 dilution. Membrane protein extract isolated from Hela cells. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15427_rabbit-sodium-potassium-atpase-alpha-1-mab-3l6f0-western-blot-206202393441..jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (3L6F0) [NBP3-15427] - Analysis of Na+/K+-ATPase in paraffin-embedded human colon using Na+/K+-ATPase Rabbit mAb at dilution of 1:400 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15427_rabbit-sodium-potassium-atpase-alpha-1-mab-3l6f0-immunohistochemistry-paraffin-305202471030..jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (3L6F0) [NBP3-15427] - Analysis of Na+/K+-ATPase in paraffin-embedded human kidney using Na+/K+-ATPase Rabbit mAb at dilution of 1:400 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15427_rabbit-sodium-potassium-atpase-alpha-1-mab-3l6f0-immunohistochemistry-paraffin-305202471139..jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (3L6F0) [NBP3-15427] - Analysis of Na+/K+-ATPase in paraffin-embedded human thyroid cancer using Na+/K+-ATPase Rabbit mAb at dilution of 1:400 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15427_rabbit-sodium-potassium-atpase-alpha-1-mab-3l6f0-immunohistochemistry-paraffin-305202471254..jpg)
![Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (3L6F0) [Sodium Potassium ATPase Alpha 1] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Sodium Potassium ATPase Alpha 1 Rabbit mAb at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15427_rabbit-sodium-potassium-atpase-alpha-1-mab-3l6f0-52202517172823.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (3L6F0) [Sodium Potassium ATPase Alpha 1] - Confocal imaging of human colon using Sodium Potassium ATPase Alpha 1 Rabbit mAb . DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.](http://images.novusbio.com/fullsize/nbp3-15427_rabbit-sodium-potassium-atpase-alpha-1-mab-3l6f0-52202517162347.jpg)
![Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (3L6F0) [Sodium Potassium ATPase Alpha 1] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Sodium Potassium ATPase Alpha 1 Rabbit mAb at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15427_rabbit-sodium-potassium-atpase-alpha-1-mab-3l6f0-52202517251984.jpg)
![Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (3L6F0) [Sodium Potassium ATPase Alpha 1] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Sodium Potassium ATPase Alpha 1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15427_rabbit-sodium-potassium-atpase-alpha-1-mab-3l6f0-52202517192982.jpg)
![Immunocytochemistry/Immunofluorescence: Neurokinin B Antibody [NBP1-92178] - Staining of human cell line U-2 OS shows localization to vesicles.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92178-img0004.jpg "Immunocytochemistry/Immunofluorescence: Neurokinin B Antibody [NBP1-92178] - Staining of human cell line U-2 OS shows localization to vesicles.")
![Immunohistochemistry-Paraffin: Neurokinin B Antibody [NBP1-92178] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-92178-img0002.jpg)
![Immunohistochemistry-Paraffin: Neurokinin B Antibody [NBP1-92178] -Staining of human Placenta shows weak cytoplasmic positivity in syncytiotrophoblast.](http://images.novusbio.com/fullsize/antibody/nbp1-92178_rabbit-polyclonal-neurokinin-b-antibody-immunohistochemistry-paraffin-22120258449..png)
![Immunohistochemistry-Paraffin: Neurokinin B Antibody [NBP1-92178] -Staining of human Liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-92178_rabbit-polyclonal-neurokinin-b-antibody-immunohistochemistry-paraffin-22120258539..png)
![Immunocytochemistry/Immunofluorescence: Neurokinin B Antibody [NBP1-92178] -Staining of Mouse brain shows moderate positivity in cells in molecular layer in the cerebellum.](http://images.novusbio.com/fullsize/antibody/nbp1-92178_rabbit-polyclonal-neurokinin-b-antibody-immunocytochemistry-immunofluorescence-22120258647..png)
![Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining in human testis and prostate tissues using NBP1-84466 antibody. Corresponding DNAI1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DNAI1-Antibody-Immunohistochemistry-Paraffin-NBP1-84466-img0019.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining in human testis and prostate tissues using NBP1-84466 antibody. Corresponding DNAI1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human fallopian tube, placenta, prostate and testis using Anti-DNAI1 antibody NBP1-84466 (A) shows similar protein distribution across tissues to independent antibody NBP1-84465 (B).](http://images.novusbio.com/fullsize/DNAI1-Antibody-Immunohistochemistry-Paraffin-NBP1-84466-img0015.jpg)
![Western Blot: DNAI1 Antibody [NBP1-84466] - Analysis in control (vector only transfected HEK293T lysate) and dNAI1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/DNAI1-Antibody-Western-Blot-NBP1-84466-img0006.jpg)
![Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human Fallopian tube shows strong positivity in cilia in glandular cells.](http://images.novusbio.com/fullsize/DNAI1-Antibody-Immunohistochemistry-Paraffin-NBP1-84466-img0014.jpg)
![Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/DNAI1-Antibody-Immunohistochemistry-Paraffin-NBP1-84466-img0016.jpg)
![Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/DNAI1-Antibody-Immunohistochemistry-Paraffin-NBP1-84466-img0017.jpg)
![Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/DNAI1-Antibody-Immunohistochemistry-Paraffin-NBP1-84466-img0018.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - A431 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-HL114-Western-Blot-NBP3-13660-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - A431 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Sodium Potassium ATPase Alpha 1 antibody [HL114] detects Sodium Potassium ATPase Alpha 1 protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: Sodium Potassium ATPase Alpha 1 stained by Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-HL114-Immunocytochemistry-Immunofluorescence-NBP3-13660-img0002.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Sodium Potassium ATPase Alpha 1 antibody [HL114] detects Sodium Potassium ATPase Alpha 1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Sodium Potassium ATPase Alpha 1 stained by Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-HL114-Immunohistochemistry-Paraffin-NBP3-13660-img0003.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-HL114-Western-Blot-NBP3-13660-img0001.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Sodium Potassium ATPase Alpha 1 antibody [HL114] detects Sodium Potassium ATPase Alpha 1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Sodium Potassium ATPase Alpha 1 stained by Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13660_rabbit-sodium-potassium-atpase-alpha-1-mab-hl114-910202420131225.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Drosophila tissue extract (5 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13660_rabbit-sodium-potassium-atpase-alpha-1-mab-hl114-9102024205691.jpg)
![A431 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium/Potassium ATPase alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13660_rabbit-sodium-potassium-atpase-alpha-1-mab-hl114-western-blot-261120251995423.jpg)