| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-28904SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-00588SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24570-25ul | |||
| Azide and BSA Free | NBP3-43844 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12900-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24571-25ul | |||
| Azide and BSA Free | NBP3-43804 | |||
![Immunocytochemistry/Immunofluorescence: Lgr5/GPR49 Antibody [NBP1-28904] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lgr5/GPR49 Antibody NBP1-28904 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-28904-img0009.jpg "Immunocytochemistry/Immunofluorescence: Lgr5/GPR49 Antibody [NBP1-28904] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lgr5/GPR49 Antibody NBP1-28904 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry: Lgr5/GPR49 Antibody [NBP1-28904] - Wnt-related genes show selective expression in the ZG of human adrenal. IHC localization of LGR5 in the ZG. IHC was performed on formalin-fixed, paraffin-embedded human adrenal sections (4 um) using a chromogen-based detection system (3,3'-Diaminobenzidine), which results in a positive brown staining. Pictures are representative of six normal adrenal sections that were used in our microarray study; four from primary hyperaldosteronism patients and two from pheochromocytoma patients. Scale bar, 500 um (low power) and 100 um (high power). C, capsule. Image collected and cropped by CiteAb from the following publication (//academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2015-1734), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-NBP1-28904-img0011.jpg)
![Immunohistochemistry: Lgr5/GPR49 Antibody [NBP1-28904] - Analysis of human small intestine.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-NBP1-28904-img0005.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody - BSA Free [NBP1-28904] - Wnt-related genes show selective expression in the ZG of human adrenal. A, Heat map of 37 genes (shown on the vertical axis), which are 5-fold or greater differentially expressed in ZG & ZF. Genes previously associated with the Wnt signaling pathway are highlighted in red. On the horizontal axis, each pair of ZG & ZF from the 20 adrenals are anonymized & numbered. Probe set & detailed microarray information are available from the National Center for Biotechnology Information Gene Expression Omnibus under accession number GSE64957. B, IHC localization of LGR5 & downstream Wnt signaling proteins in the ZG: from the canonical beta -catenin pathway, beta -catenin & LEF1, & from the noncanonical AP1/JUN pathway, c-JUN & p-c-JUN. IHC was performed on formalin-fixed, paraffin-embedded human adrenal sections (4 μm) using a chromogen-based detection system (3,3'-Diaminobenzidine), which results in a positive brown staining. Interestingly, beta -catenin staining was mostly membranous or cytoplasmic (rather than nuclear staining as highlighted by the arrows), indicating limited canonical Wnt activation. Pictures are representative of six normal adrenal sections that were used in our microarray study; four from primary hyperaldosteronism patients & two from pheochromocytoma patients. Scale bar, 500 μm (low power) & 100 μm (high power). C, capsule. Image collected & cropped by CiteAb from the following publication (//academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2015-1734), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-28904_rabbit-polyclonal-lgr5-gpr49-antibody-31020241618943.jpg)
![Wild-type (WT) and NDUFS3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48671_rabbit-ndufs3-mab-hl2097-western-blot-1411202518384124.jpg "<b>Genetic Strategies Validation. </b>Wild-type (WT) and NDUFS3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>NDUFS3 antibody [HL2097] detects NDUFS3 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.NDUFS3 stained by NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:50.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48671_rabbit-ndufs3-mab-hl2097-immunohistochemistry-paraffin-141120251835222.jpg)
![NDUFS3 antibody [HL2097] detects NDUFS3 protein at mitochondria by immunofluorescent analysis.Sample: 293T cells were fixed in ice-cold MeOH for 5 min.Green: NDUFS3 stained by NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48671_rabbit-ndufs3-mab-hl2097-immunocytochemistry-immunofluorescence-141120251830215.jpg)
![Whole zebrafish extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48671_rabbit-ndufs3-mab-hl2097-western-blot-14112025183207.jpg)
![Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48671_rabbit-ndufs3-mab-hl2097-western-blot-1411202518415211.jpg)
![Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48671_rabbit-ndufs3-mab-hl2097-western-blot-1411202518363616.jpg)
![NDUFS3 antibody [HL2097] detects NDUFS3 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.NDUFS3 stained by NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:50.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48671_rabbit-ndufs3-mab-hl2097-immunohistochemistry-paraffin-1411202518415222.jpg)
![Whole Japanese medaka extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48671_rabbit-ndufs3-mab-hl2097-western-blot-141120251829415.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Anaysis of Lgr5/GPR49 with Brain, hippocampus](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-Paraffin-NLS1236-img0015.jpg "Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Anaysis of Lgr5/GPR49 with Brain, hippocampus")
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Analysis of anti-GPR49 antibody with human, small intestine.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-Paraffin-NLS1236-img0011.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Analysis of anti-GPR49 antibody with human colon.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-Paraffin-NLS1236-img0012.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Analysis of Lgr5/GPR49 with brain, glioblastoma](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-Paraffin-NLS1236-img0013.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Analysis of Lgr5/GPR49 with Brain, Caudate](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-Paraffin-NLS1236-img0014.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody - BSA Free [NLS1236] - Immunohistochemistry of formalin-fixed, paraffin-embedded Human Brain, Cortex tissue](http://images.novusbio.com/fullsize/nls1236_rabbit-lgr5-gpr49-pab-bsa-free-10920241103115.jpg)
![Western Blot: Ankyrin Brain Antibody [NBP2-33863] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Western-Blot-NBP2-33863-img0003.jpg "Western Blot: Ankyrin Brain Antibody [NBP2-33863] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody [NBP2-33863] - Staining of human cell line RH-30 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33863-img0008.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0012.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0004.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Analysis in human cerebral cortex and skin tissues using NBP2-33863 antibody. Corresponding ANK2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0009.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0010.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0011.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human skin shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0013.jpg)
![Western Blot: Jumonji/JARID2 Antibody [NBP3-00588] - Total protein from human U2OS and NTERA-2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Jumonji (NBP3-00588) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Western-Blot-NBP3-00588-img0003.jpg "Western Blot: Jumonji/JARID2 Antibody [NBP3-00588] - Total protein from human U2OS and NTERA-2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Jumonji (NBP3-00588) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: Jumonji/JARID2 Antibody [NBP3-00588] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Jumonji/JARID2 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-00588-img0002.jpg)
![Immunohistochemistry-Paraffin: Jumonji/JARID2 Antibody [NBP3-00588] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of Jumonji / JARID2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Jumonji / JARID2 antibody generated nuclear staining.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Immunohistochemistry-Paraffin-NBP3-00588-img0001.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining in human appendix and skeletal muscle tissues using anti-NFKB2 antibody. Corresponding NFKB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining in human appendix and skeletal muscle tissues using anti-NFKB2 antibody. Corresponding NFKB2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-NFKB2 antibody NBP1-87759 (A) shows similar protein distribution across tissues to independent antibody NBP1-87760 (B).](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: NFkB p100/p52 Antibody [NBP1-87759] - Western blot analysis in human cell lines A-431 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Western-Blot-NBP1-87759-img0009.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human kidney.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0014.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human appendix shows high expression.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0006.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0007.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human lymph node.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0012.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human testis.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0013.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human cerebral cortex using Anti-NFKB2 antibody.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0015.jpg)
![Western Blot: Kir2.1 Antibody (S112) [NBP2-12900] - Western Blot analysis of Monkey COS transient cell lysate showing detection of Kir2.1 Potassium Channel protein using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112 (NBP2-12900). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody (NBP2-12900) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Kir2-1-Antibody-S112-Western-Blot-NBP2-12900-img0005.jpg "Western Blot: Kir2.1 Antibody (S112) [NBP2-12900] - Western Blot analysis of Monkey COS transient cell lysate showing detection of Kir2.1 Potassium Channel protein using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112 (NBP2-12900). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody (NBP2-12900) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: Kir2.1 Antibody (S112) [NBP2-12900] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kir2.1 Monoclonal Antibody, Clone S112 (NBP2-12900). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Kir2.1 Monoclonal Antibody (NBP2-12900) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kir2.1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Kir2-1-Antibody-S112-Immunocytochemistry-Immunofluorescence-NBP2-12900-img0007.jpg)
![Immunohistochemistry: Kir2.1 Antibody (S112) [NBP2-12900] - Immunohistochemistry analysis using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112 (NBP2-12900). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody (NBP2-12900) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Kir2-1-Antibody-S112-Immunohistochemistry-NBP2-12900-img0006.jpg)
![Immunohistochemistry: Kir2.1 Antibody (S112) [NBP2-12900] - Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear expression in the epidermis and hair follicles.](http://images.novusbio.com/fullsize/Kir2-1-Antibody-S112-Immunohistochemistry-NBP2-12900-img0003.jpg)
![Immunohistochemistry-Paraffin: LEDGF Antibody [NB110-40560] - Detection of human LEDGF/p75 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-LEDGF/p75 antibody (NB110-40560). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunohistochemistry-Paraffin-NB110-40560-img0005.jpg "Immunohistochemistry-Paraffin: LEDGF Antibody [NB110-40560] - Detection of human LEDGF/p75 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-LEDGF/p75 antibody (NB110-40560). Secondary: HRP-conjugated goat anti-rabbit IgG.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: LEDGF Antibody [NB110-40560] - Detection of human LEDGF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LEDGF antibody NB110-40560 used for IP at 3 ug per reaction. LEDGF was also immunoprecipitated by rabbit anti-LEDGF antibody NB110-40561. For blotting immunoprecipitated LEDGF, NB110-40560 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunoprecipitation-NB110-40560-img0006.jpg)
![Western Blot: LEDGF Antibody [NB110-40561] - Detection of Human LEDGF/p75 on HeLa whole cell lysate using NB110-40561. LEDGF/p75 was also immunoprecipitated by another rabbit anti-LEDGF/p75 antibody.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Western-Blot-NB110-40561-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: LEDGF Antibody [NB110-40561] - Detection of Human LEDGF/p75 on HeLa whole cell lysate using NB110-40561. LEDGF/p75 was also immunoprecipitated by another rabbit anti-LEDGF/p75 antibody.")
