| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-28904SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24570-25ul | |||
| Azide and BSA Free | NBP3-43844 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24571-25ul | |||
| Azide and BSA Free | NBP3-43804 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37513-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85462-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44215 | |||
| BSA Free | NBP2-61435-25ul | |||
![Immunocytochemistry/Immunofluorescence: Lgr5/GPR49 Antibody [NBP1-28904] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lgr5/GPR49 Antibody NBP1-28904 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-28904-img0009.jpg "Immunocytochemistry/Immunofluorescence: Lgr5/GPR49 Antibody [NBP1-28904] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lgr5/GPR49 Antibody NBP1-28904 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry: Lgr5/GPR49 Antibody [NBP1-28904] - Wnt-related genes show selective expression in the ZG of human adrenal. IHC localization of LGR5 in the ZG. IHC was performed on formalin-fixed, paraffin-embedded human adrenal sections (4 um) using a chromogen-based detection system (3,3'-Diaminobenzidine), which results in a positive brown staining. Pictures are representative of six normal adrenal sections that were used in our microarray study; four from primary hyperaldosteronism patients and two from pheochromocytoma patients. Scale bar, 500 um (low power) and 100 um (high power). C, capsule. Image collected and cropped by CiteAb from the following publication (//academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2015-1734), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-NBP1-28904-img0011.jpg)
![Immunohistochemistry: Lgr5/GPR49 Antibody [NBP1-28904] - Analysis of human small intestine.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-NBP1-28904-img0005.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody - BSA Free [NBP1-28904] - Wnt-related genes show selective expression in the ZG of human adrenal. A, Heat map of 37 genes (shown on the vertical axis), which are 5-fold or greater differentially expressed in ZG & ZF. Genes previously associated with the Wnt signaling pathway are highlighted in red. On the horizontal axis, each pair of ZG & ZF from the 20 adrenals are anonymized & numbered. Probe set & detailed microarray information are available from the National Center for Biotechnology Information Gene Expression Omnibus under accession number GSE64957. B, IHC localization of LGR5 & downstream Wnt signaling proteins in the ZG: from the canonical beta -catenin pathway, beta -catenin & LEF1, & from the noncanonical AP1/JUN pathway, c-JUN & p-c-JUN. IHC was performed on formalin-fixed, paraffin-embedded human adrenal sections (4 μm) using a chromogen-based detection system (3,3'-Diaminobenzidine), which results in a positive brown staining. Interestingly, beta -catenin staining was mostly membranous or cytoplasmic (rather than nuclear staining as highlighted by the arrows), indicating limited canonical Wnt activation. Pictures are representative of six normal adrenal sections that were used in our microarray study; four from primary hyperaldosteronism patients & two from pheochromocytoma patients. Scale bar, 500 μm (low power) & 100 μm (high power). C, capsule. Image collected & cropped by CiteAb from the following publication (//academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2015-1734), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-28904_rabbit-polyclonal-lgr5-gpr49-antibody-31020241618943.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Anaysis of Lgr5/GPR49 with Brain, hippocampus](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-Paraffin-NLS1236-img0015.jpg "Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Anaysis of Lgr5/GPR49 with Brain, hippocampus")
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Analysis of anti-GPR49 antibody with human, small intestine.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-Paraffin-NLS1236-img0011.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Analysis of anti-GPR49 antibody with human colon.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-Paraffin-NLS1236-img0012.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Analysis of Lgr5/GPR49 with brain, glioblastoma](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-Paraffin-NLS1236-img0013.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1236] - Analysis of Lgr5/GPR49 with Brain, Caudate](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-Paraffin-NLS1236-img0014.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody - BSA Free [NLS1236] - Immunohistochemistry of formalin-fixed, paraffin-embedded Human Brain, Cortex tissue](http://images.novusbio.com/fullsize/nls1236_rabbit-lgr5-gpr49-pab-bsa-free-10920241103115.jpg)
![Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of RAF1 expression in transfected 293T cell line by RAF1 monoclonal antibody (M03), clone 1H4. Lane 1: RAF1 transfected lysatE (73.1 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Raf-1-Antibody-1H4-Western-Blot-H00005894-M03-img0007.jpg "Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of RAF1 expression in transfected 293T cell line by RAF1 monoclonal antibody (M03), clone 1H4. Lane 1: RAF1 transfected lysatE (73.1 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Western blot analysis of RAF1 over-expressed 293 cell line, cotransfected with RAF1 Validated Chimera RNAi or non-transfected control. Blot probed with H00005894-M03. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize/Raf-1-Antibody-1H4-Western-Blot-H00005894-M03-img0002.jpg)
![Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of RAF1 expression in HeLa.](http://images.novusbio.com/fullsize/Raf-1-Antibody-1H4-Western-Blot-H00005894-M03-img0005.jpg)
![Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of RAF1 expression in rat brain.](http://images.novusbio.com/fullsize/Raf-1-Antibody-1H4-Western-Blot-H00005894-M03-img0006.jpg)
![Proximity Ligation Assay: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of protein-protein interactions between BAD and RAF1. HeLa cells were stained with anti-BAD rabbit purified polyclonal 1:1200 and anti-RAF1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction compl](http://images.novusbio.com/fullsize/Raf-1-Antibody-1H4-Proximity-Ligation-Assay-H00005894-M03-img0003.jpg)
![Proximity Ligation Assay: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of protein-protein interactions between PDGFRB and RAF1. Huh7 cells were stained with anti-PDGFRB rabbit purified polyclonal 1:600 and anti-RAF1 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction](http://images.novusbio.com/fullsize/Raf-1-Antibody-1H4-Proximity-Ligation-Assay-H00005894-M03-img0004.jpg)
![Western Blot: Raf-1 Antibody (4G4) [NBP2-37513] - Western blot analysis using RAF1 mouse mAb against HeLa (1), A431 (2), HepG (3), and SW620 (4)cell lysate.](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-Western-Blot-NBP2-37513-img0003.jpg "Western Blot: Raf-1 Antibody (4G4) [NBP2-37513] - Western blot analysis using RAF1 mouse mAb against HeLa (1), A431 (2), HepG (3), and SW620 (4)cell lysate.")
![Immunohistochemistry: Raf-1 Antibody (4G4) [NBP2-37513] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RAF1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-Immunohistochemistry-NBP2-37513-img0005.jpg)
![Flow Cytometry: Raf-1 Antibody (4G4) [NBP2-37513] - Flow cytometric analysis of MCF-7 cells using RAF1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-Flow-Cytometry-NBP2-37513-img0004.jpg)
![Western Blot: Raf-1 Antibody (4G4) [NBP2-37513] - Western blot analysis using RAF1 mAb against human RAF1 (AA: 198-407) recombinant protein. (Expected MW is 48.6 kDa)](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-Western-Blot-NBP2-37513-img0002.jpg)
![ELISA: Raf-1 Antibody (4G4) [NBP2-37513] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-ELISA-NBP2-37513-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Raf-1 Antibody [NBP1-85462] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody stainig is shown in green.](http://images.novusbio.com/fullsize/Raf-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85462-img0006.jpg "Immunocytochemistry/Immunofluorescence: Raf-1 Antibody [NBP1-85462] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody stainig is shown in green.")
![Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462] - Staining of human testis shows weak positivity in nuclear speckles in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Raf-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85462-img0011.jpg)
![Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462] - Staining of human cerebellum shows moderate positivity in nuclear speckles in Purkinje cells.](http://images.novusbio.com/fullsize/Raf-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85462-img0008.jpg)
![Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462] - Staining of human cervix shows moderate positivity in nuclear speckles in squamous epithelial cells.](http://images.novusbio.com/fullsize/Raf-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85462-img0009.jpg)
![Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462] - Staining of human rectum shows moderate positivity in nuclear speckles in glandular cells.](http://images.novusbio.com/fullsize/Raf-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85462-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Calpain 5 Antibody [NBP1-32222] - Paraformaldehyde-fixed HeLa, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Calpain-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32222-img0002.jpg "Immunocytochemistry/Immunofluorescence: Calpain 5 Antibody [NBP1-32222] - Paraformaldehyde-fixed HeLa, using antibody at 1:100 dilution.")
![Western Blot: Calpain 5 Antibody [NBP1-32222] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [N1C1] (NBP1-32222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32222_rabbit-polyclonal-calpain-5-antibody-91020242001055.jpg)
![Western Blot: Calpain 5 Antibody [NBP1-32222] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [N1C1] (NBP1-32222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32222_rabbit-polyclonal-calpain-5-antibody-91020242021918.jpg)
![Western Blot: Calpain 5 Antibody [NBP1-32222] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [N1C1] (NBP1-32222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32222_rabbit-polyclonal-calpain-5-antibody-91020242011455.jpg)
![Immunohistochemistry-Paraffin: Calpain 5 Antibody (HL2432) - Azide and BSA Free [NBP3-25394] - Calpain 5 antibody [HL2432] detects Calpain 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Calpain 5 stained by Calpain 5 antibody [HL2432] (NBP3-25394) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25394_rabbit-calpain-5-mab-hl2432-azide-and-bsa-free-2120249425418.jpg "Immunohistochemistry-Paraffin: Calpain 5 Antibody (HL2432) - Azide and BSA Free [NBP3-25394] - Calpain 5 antibody [HL2432] detects Calpain 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Calpain 5 stained by Calpain 5 antibody [HL2432] (NBP3-25394) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Calpain 5 Antibody (HL2432) - Azide and BSA Free [NBP3-25394] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [HL2432] (NBP3-25394) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25394_rabbit-calpain-5-mab-hl2432-azide-and-bsa-free-2120249425431.jpg)
![Immunohistochemistry-Paraffin: Calpain 5 Antibody (HL2432) - Azide and BSA Free [NBP3-25394] - Calpain 5 antibody [HL2432] detects secreted Calpain 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat stomach. Calpain 5 stained by Calpain 5 antibody [HL2432] (NBP3-25394) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25394_rabbit-calpain-5-mab-hl2432-azide-and-bsa-free-2120249355515.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Calpain 5 Antibody (HL2432) - Azide and BSA Free [NBP3-25394] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [HL2432] (NBP3-25394) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25394_rabbit-calpain-5-mab-hl2432-azide-and-bsa-free-212024939195.jpg)
![Western Blot: LHX2 Antibody (CL6137) [NBP2-61435] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/LHX2-Antibody-CL6137-Western-Blot-NBP2-61435-img0005.jpg "Western Blot: LHX2 Antibody (CL6137) [NBP2-61435] - Analysis in human cerebral cortex tissue.")
![Immunohistochemistry-Paraffin: LHX2 Antibody (CL6137) [NBP2-61435] - Staining of mouse embryo E14 shows strong nuclear immunoreactivity in the developing cortex and sensory organs.](http://images.novusbio.com/fullsize/LHX2-Antibody-CL6137-Immunohistochemistry-Paraffin-NBP2-61435-img0001.jpg)
![Western Blot: LHX2 Antibody (CL6137) [NBP2-61435] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/LHX2-Antibody-CL6137-Western-Blot-NBP2-61435-img0004.jpg)
![Immunohistochemistry-Paraffin: IP3R3 Antibody [NBP1-21399] - Section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-IP3R3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/IP3R3-Antibody-Immunohistochemistry-Paraffin-NBP1-21399-img0004.jpg "Immunohistochemistry-Paraffin: IP3R3 Antibody [NBP1-21399] - Section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-IP3R3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Immunoprecipitation: IP3R3 Antibody [NBP1-21399] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-IP3R3 antibody used for IP at 3 mcg/mg lysate. IP3R3 was also immunoprecipitated by rabbit anti-IP3R3 antibody NBP1-21400 which recognizes a downstream e](http://images.novusbio.com/fullsize/IP3R3-Antibody-Immunoprecipitation-NBP1-21399-img0003.jpg)
