| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-89719SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13172-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-140-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB300-101SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-210SS | |||
| BSA Free | NB500-210-0.1ml | |||
| Azide and BSA Free | NBP2-80614 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05197SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-270SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-54467SS | |||
| Azide and BSA Free | NBP2-80596 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-201SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33184 | |||
![Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NB110-89719] - Ki67 antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunocytochemistry-Immunofluorescence-NB110-89719-img0006.jpg "Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NB110-89719] - Ki67 antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB110-89719] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse intestine using 1:200 dilution of rabbit anti-KI67 antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This antibody generated a specific nuclear staining in epithelial cells and the staining was more intense in the cells close to the bases of crypts. Weak to moderate positivity was found at the cytoplasmic level also.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunohistochemistry-Paraffin-NB110-89719-img0008.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody - BSA Free [NB110-89719] - Ki67/MKI67 Antibody [NB110-89719] - Effect of saireito on the suppression of cell proliferation induced by 5-fluorouracil (5-FU) in mouse small intestines. 5-FU (50 mg/kg) was injected i.p. while saireito (1000 mg/kg) was administered p.o. twice, 30 min before and 8 h after 5-FU injection. The jejunum was excised, sectioned, and Ki67 immunostaining was performed. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0116213), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunohistochemistry-Paraffin-NB110-89719-img0010.jpg)
![Immunohistochemistry: Ki67/MKI67 Antibody [NB110-89719] - Detection of Ki67 in formalin-fixed paraffin embedded mouse intestine using NB110-89719.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunohistochemistry-NB110-89719-img0009.jpg)
![Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB110-89719] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Ki-67 (NB110-89719) used at a dilution of 1:250.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunohistochemistry-Paraffin-NB110-89719-img0001.jpg)
![Immunohistochemistry: Ki67/MKI67 Antibody [NB110-89719] - Immunohistochemical analysis of mouse spleen.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunohistochemistry-NB110-89719-img0007.jpg)
![Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB110-89719] - IHC analysis of Ki67 in mouse cornea. Image courtesy of product review by Bo-Yie Chen.](http://images.novusbio.com/fullsize/Ki67-MKI67-Antibody-Immunohistochemistry-Paraffin-NB110-89719-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody - BSA Free [NB110-89719] - The increase in iCM number with SB treatment is not due to increased transgene expression or changes in cell proliferation.Evaluation of gene expression of the transgenes at Day 2 via qPCR is not significantly different between +DMSO (black) control & +SB treatment groups (white, A). Evaluation of overall cell proliferation with Ki67 staining in MEFs with time for +DMSO (black) & +SB treatment groups (grey) (B). A time course evaluation of Troponin T (green) & Ki67 (red) did any dual labeled cells for either the +DMSO (top row) & +SB (bottom row) treated groups (C). Scale bar is 100 µM. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0089678), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89719_rabbit-polyclonal-ki67-mki67-antibody-310202415345318.jpg)
![Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - GADD153/CHOP Antibody [NBP2-13172] - NASTRp induces ER stress and eventually leads to cell death with Bim up-regulation. NASTRp induced ER stress and activated UPR led to apoptosis with Bim induction in NSCLC cell lines. Cells were treated with the indicated concentrations of NASTRp for 24 hours and cell lysates were subjected to SDS-PAGE/Western blot analysis using the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0122628) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Western-Blot-NBP2-13172-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - GADD153/CHOP Antibody [NBP2-13172] - NASTRp induces ER stress and eventually leads to cell death with Bim up-regulation. NASTRp induced ER stress and activated UPR led to apoptosis with Bim induction in NSCLC cell lines. Cells were treated with the indicated concentrations of NASTRp for 24 hours and cell lysates were subjected to SDS-PAGE/Western blot analysis using the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0122628) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: GADD153/CHOP Antibody [NBP2-13172] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-GADD153/CHOP Antibody NBP2-13172 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13172-img0008.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - GADD153/CHOP Antibody [NBP2-13172] - Effects of psoralen on ER-stress related protein expression in SMMC7721. Western blot assays showing the effects of psoralen and thapsigargin on ER-stress related protein expression. Image collected and cropped by CiteAb from the following publication (//biolres.biomedcentral.com/articles/10.1186/s40659-019-0241-8), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Western-Blot-NBP2-13172-img0007.jpg)
![Immunohistochemistry: GADD153/CHOP Antibody [NBP2-13172] - Mouse liver tissue. Antigen retreival: Citrate buffer pH=6.0,Blocking: 10% BSA in 0.1%Tween in TBS, Primary antibody: 1:100 in 1% BSA in 0.1%Tween in TBS,Incubation: Overnight at 4C, Secondary antibody: 1:200 incubation for 1hr at room temperature, AB complex: 1:100 for 1hr at room temprature Develpment DAB. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Immunohistochemistry-NBP2-13172-img0005.jpg)
![Flow Cytometry: GADD153/CHOP Antibody [NBP2-13172] - An intracellular stain was performed on SK-MEL-28 cells with GADD153/CHOP Antibody NBP2-13172F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Flow-Cytometry-NBP2-13172-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: GADD153/CHOP Antibody [NBP2-13172] - CHOP/GADD153 Antibody [NBP2-13172] - WB analysis of CHOP/GADD153 in tunicamycin treated (+) and untreated (-) HeLa cell lysate.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Western-Blot-NBP2-13172-img0003.jpg)
![Immunohistochemistry: GADD153/CHOP Antibody [NBP2-13172] - IHC staining of CHOP/GADD153 in mouse colon using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-Immunohistochemistry-NBP2-13172-img0002.jpg)
![Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - Effects of psoralen on ER-stress related protein expression in SMMC7721. a Western blot assays showing the effects of psoralen & thapsigargin on ER-stress related protein expression. b Western blot assays showing the effects of TUDC on the expression of ER-stress related protein induced by PSO. c The mRNA levels of GRP78 & DDIT3 with TUDC under PSO & TG treatment. Values are mean ± SD (n = 3) & * is means compared to the Con group, **P < 0.01 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31277690), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-13172_rabbit-polyclonal-gadd153-chop-antibody-310202415291942.jpg)
![Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - Reduction of fibronectin prevents Dex-induced ER stress in human GTM-3 cells. (A) GTM3 cells were transfected with a plasmid expressing CRISPR-Cas9 targeting fibronectin (CR-FN) & then treated with Veh or Dex for 48 hours. Fibronectin knockdown partially reduced ER stress as evident from reduced GRP78 & CHOP levels after Dex treatment (n = 2). (B) Human GTM3 cells were treated with Dex (100 nM) with or without CR-FN for 48 hours. Fixed cells were stained for fibronectin & KDEL to examine Dex-induced fibronectin & ER stress. Fibronectin knockdown reduced fibronectin & ER stress in Dex-treated TM cells (n = 2). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-14938-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-13172_rabbit-polyclonal-gadd153-chop-antibody-310202415395954.jpg)
![Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - TM cells are sensitive to ER stress from Dex-induced abnormal ECM compared to other ocular cells: Primary human TM cells (A), RPE (B) & corneal fibroblasts (C) were treated with Veh or Dex for 3 days & immunostained for fibronectin & KDEL. Although fibronectin was increased in all of these cell types, ER stress was only observed in TM cells. (D) Human GTM3 or ARPE-19 cells were treated with Veh or Dex for 3 days. Fibronectin & chronic ER stress markers ATF4 & CHOP were examined in cellular lysates (n = 2). Although Dex increased fibronectin in both GTM3 & ARPE-19 cells, chronic ER stress was only observed in GTM3 cells indicating that TM cells are more prone to ER stress from Dex-induced ECM accumulation. (E) GTM-3 or ARPE-19 cells were treated with cellular fibronectin (cFn) for 2 days & ER stress was examined by Western blot analysis. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-14938-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-13172_rabbit-polyclonal-gadd153-chop-antibody-310202416121940.jpg)
![Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - Overexpression of extracellular fibronectin induces ER stress in primary human TM cells. (A) Primary human TM cells (n = 2 strains) were treated with cellular fibronectin (10 ug/ml) for 48 hours & cellular lysates were analyzed for ER stress markers. (B) Primary human TM cells (n = 2 strains) were treated with cellular fibronectin (10 ug/ml) for 48 hours & fixed cells were stained with fibronectin & KDEL antibodies. Cellular fibronectin increased extracellular fibronectin & ER stress in GTM3 cells (n = 2). Scale bar = 50 μm. (C) GTM3 cells were transduced with fibronectin activation lentiviral particles for 48 hours & stained with fibronectin & KDEL antibodies. Increased fibronectin expression alone is sufficient to induce ER stress in TM cells (n = 2). Scale bar = 50 μm. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-14938-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-13172_rabbit-polyclonal-gadd153-chop-antibody-310202416113515.jpg)
![Western Blot: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - NASTRp induces ER stress & eventually leads to cell death with Bim up-regulation.(A) qRT-PCR analysis of biomarkers of ER stress/UPR related genes in NCI-H441 treated with 20 μM NASTRp in time course manner. (B) NASTRp induced ER stress & activated UPR led to apoptosis with Bim induction in NSCLC cell lines. Cells were treated with the indicated concentrations of NASTRp for 24 hours & cell lysates were subjected to SDS-PAGE/Western blot analysis using the indicated antibodies. (C) Upregulation of CHOP by NASTRp. NCI-H441 cells were treated with 20 NASTRp for 0–24 hours. At the indicated time points, cells were fixed & subjected to immunofluorescence assay with anti-CHOP antibody. Cell images were microphotographed using fluorescence microscopy at 60X magnification. Scale bars: white; 10 μm. (D) TUNEL-positive cells in NASTRp-treated (for 48 hours) A549 cells were counted & analyzed as relative % of TUNEL-positive cells. *P < 0.05 versus vehicle. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122628), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-13172_rabbit-polyclonal-gadd153-chop-antibody-31020241613624.jpg)
![Immunocytochemistry/ Immunofluorescence: GADD153/CHOP Antibody - BSA Free [NBP2-13172] - NASTRp induces ER stress & eventually leads to cell death with Bim up-regulation.(A) qRT-PCR analysis of biomarkers of ER stress/UPR related genes in NCI-H441 treated with 20 μM NASTRp in time course manner. (B) NASTRp induced ER stress & activated UPR led to apoptosis with Bim induction in NSCLC cell lines. Cells were treated with the indicated concentrations of NASTRp for 24 hours & cell lysates were subjected to SDS-PAGE/Western blot analysis using the indicated antibodies. (C) Upregulation of CHOP by NASTRp. NCI-H441 cells were treated with 20 NASTRp for 0–24 hours. At the indicated time points, cells were fixed & subjected to immunofluorescence assay with anti-CHOP antibody. Cell images were microphotographed using fluorescence microscopy at 60X magnification. Scale bars: white; 10 μm. (D) TUNEL-positive cells in NASTRp-treated (for 48 hours) A549 cells were counted & analyzed as relative % of TUNEL-positive cells. *P < 0.05 versus vehicle. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122628), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-13172_rabbit-polyclonal-gadd153-chop-antibody-31020241612191.jpg)
![Western Blot: alpha-Internexin Antibody (2E3) [NB300-140] - Analysis of different tissue lysates using alpha-Internexin, dilution 1:10000 (Red): [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord lysate. The antibody reveals the alpha-Internexin protein with an apparent molecular weight of 64-66kDa, with some variability across species.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-2E3-Western-Blot-NB300-140-img0005.jpg "Western Blot: alpha-Internexin Antibody (2E3) [NB300-140] - Analysis of different tissue lysates using alpha-Internexin, dilution 1:10000 (Red): [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord lysate. The antibody reveals the alpha-Internexin protein with an apparent molecular weight of 64-66kDa, with some variability across species.")
![Immunocytochemistry/Immunofluorescence: alpha-Internexin Antibody (2E3) [NB300-140] - Hippocampal neurons in tissue culture with neurofilament alpha internexin (red), using NB300-140 and GFAP (green), using NB300-141.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-2E3-Immunocytochemistry-Immunofluorescence-NB300-140-img0003.jpg)
![Immunohistochemistry: alpha-Internexin Antibody (2E3) [NB300-140] - Section of rat facial nucleus 7 days following axotomy. These neurons are capable of regenerating their axons and also, concomitant with regeneration, strongly upregulate alpha-Internexin in their perikarya. Other central neurons which are not able to regenerate their axons do not upregulate this protein after axotomy and untreated facial neurons normally show only very low levels of alpha-Internexin. Both findings suggest that alpha-Internexin has a role in axonal regeneration.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-2E3-Immunohistochemistry-NB300-140-img0006.jpg)
![Simple Western: alpha-Internexin Antibody (2E3) [NB300-140] - Simple Western lane view shows a specific band for alpha Internexin in 0.5 mg/ml of SH-SY5Y lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-2E3-Simple-Western-NB300-140-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-101] - IF analysis of Neurokinin 1 Receptor in rat brain tissue. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/Neurokinin-1-Receptor-Antibody-Immunocytochemistry-Immunofluorescence-NB300-101-img0007.jpg "Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-101] - IF analysis of Neurokinin 1 Receptor in rat brain tissue. Image courtesy of anonymous customer product review.")
![Immunohistochemistry: Neurokinin 1 Receptor Antibody [NB300-101] - Immunohistochemical analysis of Neurokinin 1 Receptor in mouse brain (selected cells, axon & dendrites) using NB300-101.](http://images.novusbio.com/fullsize/Neurokinin-1-Receptor-Antibody-Immunohistochemistry-NB300-101-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-101] - Confocal image of SPR reactivity using polyclonal anti-NK-1 Receptor (NB 300-101) in a lamina III neuron in the spinal cord of a rat.](http://images.novusbio.com/fullsize/Neurokinin-1-Receptor-Antibody-Immunocytochemistry-Immunofluorescence-NB300-101-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-101] - IF analysis of Neurokinin 1 Receptor in rat brain tissue. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/Neurokinin-1-Receptor-Antibody-Immunocytochemistry-Immunofluorescence-NB300-101-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - High magnification confocal image of HeLa cells stained with fibrillarin antibody, dilution 1:100 from concentrated tissue culture media (Green), and costained with chicken vimentin pAb (Red), dilution 1:1,000. Nuclear DNA is revealed with the DAPI stain (Blue). The fibrillarin antibody shows strong staining of nucleoli in the nucleus, while the vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunocytochemistry-Immunofluorescence-NB300-269-img0008.jpg "Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - High magnification confocal image of HeLa cells stained with fibrillarin antibody, dilution 1:100 from concentrated tissue culture media (Green), and costained with chicken vimentin pAb (Red), dilution 1:1,000. Nuclear DNA is revealed with the DAPI stain (Blue). The fibrillarin antibody shows strong staining of nucleoli in the nucleus, while the vimentin antibody reveals cytoplasmic intermediate filaments.")
![Western Blot: Fibrillarin Antibody (38F3) [NB300-269] - Analysis of lysates of cell fractions probed with Fibrillarin antibody, dilution 1:500 (Green): [1] protein standard, [2] C6 cytosol, [3] C6 nuclear, [4] HEK293 cytosol, [5] HEK293 nuclear, [6] NIH-3T3 cytosol and [7] NIH-3T3 nuclear fractions. The band at 37kDa corresponds to the fibrillarin protein detected exclusively in the nuclear fractions.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Western-Blot-NB300-269-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - The nuclei of hth mutants have dispersed rDNA foci and ectopic nucleoli and produce more rRNA. A) Wild type nuclei stained with anti-Fibrillarin (red) and a specific probe that recognizes the rDNA genomic region (green). B) hth mutant nuclei stained as in A. The mutant nuclei exhibit an increased number of nucleoli with different sizes. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120662), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunocytochemistry-Immunofluorescence-NB300-269-img0012.jpg)
![Immunohistochemistry-Paraffin: Fibrillarin Antibody (38F3) [NB300-269] - IHC staining of Fibrillarin in human ovarian cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunohistochemistry-Paraffin-NB300-269-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - Mouse embryonic fibroblast fibrillarin stained with fibrillarin antibody and Alexa-594 secondary antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunocytochemistry-Immunofluorescence-NB300-269-img0013.jpg)
![Flow Cytometry: Fibrillarin Antibody (38F3) [NB300-269] - Fibrillarin antibody was tested at 1:400 in HEK293 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Flow-Cytometry-NB300-269-img0004.jpg)
![Western Blot: Fibrillarin Antibody (38F3) [NB300-269] - Western blot analysis of Fibrillarin expression on yeast protein extracts using NB300-269.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Western-Blot-NB300-269-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - Fibrillarin immunofluorescence in fibroblasts. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunocytochemistry-Immunofluorescence-NB300-269-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - IF Confocal analysis of MCF7 cells using Fibrillarin antibody (NB300-269, 1:5). An Alexa Fluor 488-conjugated Goat to mouse IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunocytochemistry-Immunofluorescence-NB300-269-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - The nuclei of hth mutants have dispersed rDNA foci & ectopic nucleoli & produce more rRNA.A) Wild type nuclei stained with anti-Fibrillarin (red) & a specific probe that recognizes the rDNA genomic region (green). B) hth mutant nuclei stained as in A. The mutant nuclei exhibit an increased number of nucleoli with different sizes. C) Quantification of nuclear dots marked with both the rDNA probe & anti-Fibrillarin in wild type & mutant nuclei. 45 nuclei were quantified for each genotype. (p-value: 0,00010132, calculated using T-Test). D) qRT-PCR comparing the levels of 18S RNA transcripts in wild type & Dfhth mutant embryos. The experiment was performed three independent times to be significant (p-value: 0,01046, calculated using T-Test). Error bars in the graph show the standard deviation & have being calculated as in Fig. 1E. The experiment was normalized using the bicoid (bcd) mRNA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25794008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-269_mouse-monoclonal-fibrillarin-antibody-38f3-310202416205116.jpg)
![Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - The nuclei of hth mutants have dispersed rDNA foci & ectopic nucleoli & produce more rRNA.A) Wild type nuclei stained with anti-Fibrillarin (red) & a specific probe that recognizes the rDNA genomic region (green). B) hth mutant nuclei stained as in A. The mutant nuclei exhibit an increased number of nucleoli with different sizes. C) Quantification of nuclear dots marked with both the rDNA probe & anti-Fibrillarin in wild type & mutant nuclei. 45 nuclei were quantified for each genotype. (p-value: 0,00010132, calculated using T-Test). D) qRT-PCR comparing the levels of 18S RNA transcripts in wild type & Dfhth mutant embryos. The experiment was performed three independent times to be significant (p-value: 0,01046, calculated using T-Test). Error bars in the graph show the standard deviation & have being calculated as in Fig. 1E. The experiment was normalized using the bicoid (bcd) mRNA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25794008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-269_mouse-monoclonal-fibrillarin-antibody-38f3-310202416205175.jpg)
![Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - The nuclei of hth mutants have dispersed rDNA foci & ectopic nucleoli & produce more rRNA.A) Wild type nuclei stained with anti-Fibrillarin (red) & a specific probe that recognizes the rDNA genomic region (green). B) hth mutant nuclei stained as in A. The mutant nuclei exhibit an increased number of nucleoli with different sizes. C) Quantification of nuclear dots marked with both the rDNA probe & anti-Fibrillarin in wild type & mutant nuclei. 45 nuclei were quantified for each genotype. (p-value: 0,00010132, calculated using T-Test). D) qRT-PCR comparing the levels of 18S RNA transcripts in wild type & Dfhth mutant embryos. The experiment was performed three independent times to be significant (p-value: 0,01046, calculated using T-Test). Error bars in the graph show the standard deviation & have being calculated as in Fig. 1E. The experiment was normalized using the bicoid (bcd) mRNA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25794008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-269_mouse-monoclonal-fibrillarin-antibody-38f3-310202416205111.jpg)
![Western Blot: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - ALM induces HIF-1alpha-dependent apoptosis in PC3 cells. PC3 cells were treated with ALM for 24 h and 48 h at 0.1 uM under normoxia and hypoxia, followed by Western blot for caspase-3. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/5/439), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-Western-Blot-NB500-210-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - ALM induces HIF-1alpha-dependent apoptosis in PC3 cells. PC3 cells were treated with ALM for 24 h and 48 h at 0.1 uM under normoxia and hypoxia, followed by Western blot for caspase-3. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/5/439), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Simple Western: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Lane view shows a specific band for Caspase 3 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-Simple-Western-NB500-210-img0005.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Analysis of a FFPE human spleen section using 1:200 dilution of . The staining was developed using HRP conjugated anti-mouse secondary antibody and DAB reagent. This Caspase 3 antibody generated a specific staining in the cytoplasm of various spleenocytes.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-Immunohistochemistry-Paraffin-NB500-210-img0007.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Caspase-3 was detected in immersion fixed paraffin-embedded sections of human bladder 1:300 dilution of mouse monoclonal Caspase-3 Antibody (CPP32 4-1-18) (NB500-210, Novus Biologicals), for 1 hour at room temperature followed by anti-mouse IgG VisUCyte HRP polymer(VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-Immunohistochemistry-Paraffin-NB500-210-img0006.jpg)
![Immunohistochemistry-Frozen: Caspase-3 Antibody (CPP32 4-1-18) - BSA Free [NB500-210] - Analysis of murine spleen with Caspase-3 (green), CD3 (red) and DAPI (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-BSA-Free-Immunohistochemistry-Frozen-NB500-210-img0009.jpg)
![Western Blot: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Detection of Caspase (19 and 35 kDa) from HEK293 cell extract using (NB500-210). Lanes 1 and 2 contain inactive and active Caspase, respectively.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-Western-Blot-NB500-210-img0004.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (CPP32 4-1-18) [NB500-210] - Rat epithelial cells of the tongue base. Antigen retrieval method: Citrate buffer.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-CPP32-4-1-18-Immunohistochemistry-Paraffin-NB500-210-img0001.jpg)
![ELISA: Human HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62766] - Samples were spiked with high concentrations of Human HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-62766_human-hmgb1-hmg-1-elisa-kit-colorimetric-13220241681411.jpg "ELISA: Human HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62766] - Samples were spiked with high concentrations of Human HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62766] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-62766_human-hmgb1-hmg-1-elisa-kit-colorimetric-43202495028.jpg)
![Immunohistochemistry Free-Floating: GFAP Antibody (5C10) [NBP1-05197] - Analysis of rat cerebellum section stained with mouse GFAP mAb, dilution 1:1,000 (Green), costained with rabbit neurofilament NF-L pAb, dilution 1:2,000 (Red). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with antibodies. The GFAP antibody stains a network of astroglial cells, while the NF-L antibody labels neuronal cells and their processes.](http://images.novusbio.com/fullsize/GFAP-Antibody-5C10-Immunohistochemistry-Free-Floating-NBP1-05197-img0007.jpg "Immunohistochemistry Free-Floating: GFAP Antibody (5C10) [NBP1-05197] - Analysis of rat cerebellum section stained with mouse GFAP mAb, dilution 1:1,000 (Green), costained with rabbit neurofilament NF-L pAb, dilution 1:2,000 (Red). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with antibodies. The GFAP antibody stains a network of astroglial cells, while the NF-L antibody labels neuronal cells and their processes.")
![Simple Western: GFAP Antibody (5C10) [NBP1-05197] - Simple Western lane view shows a specific band for GFAP in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GFAP-Antibody-5C10-Simple-Western-NBP1-05197-img0004.jpg)
![Immunohistochemistry: GFAP Antibody (5C10) [NBP1-05197] - The immunofluorescence of TLR7 recognized by Alexa 488, green (NBP2-24906). GFAP recognized by Alexa 594, red. NEUN recognized by Alexa 633, (blue) and merged image in the hippocampal region. NeuN and GFAP were applied to show the distribution of TLR7 within neuronal and supportive tissue populations. Scale bar 80 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0222818) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GFAP-Antibody-5C10-Immunohistochemistry-NBP1-05197-img0008.jpg)
![Immunohistochemistry: GFAP Antibody (5C10) [NBP1-05197] - Analysis of rat cerebellum section stained with mouse mAb to GFAP, NBP1-05197, dilution 1:1,000, in green, costained with rabbit pAb to neurofilament NF-L, dilution 1:2,000, in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP1-05197 antibody stains a network of astroglial cells, while the NF-L antibody labels neuronal cells and their processes.](http://images.novusbio.com/fullsize/GFAP-Antibody-5C10-Immunocytochemistry-Immunofluorescence-NBP1-05197-img0005.jpg)
![Western Blot: GFAP Antibody (5C10) [NBP1-05197] - Analysis of whole tissue lysates using mouse mAb to GFAP, NBP1-05197, dilution 1:2,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 50kDa corresponds to the GFAP protein.](http://images.novusbio.com/fullsize/GFAP-Antibody-5C10-Western-Blot-NBP1-05197-img0006.jpg)
![Simple Western: Park7/DJ-1 Antibody [NB300-270] - Simple Western lane view shows a specific band for Park7 (DJ-1) in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-Simple-Western-NB300-270-img0009.jpg "Simple Western: Park7/DJ-1 Antibody [NB300-270] - Simple Western lane view shows a specific band for Park7 (DJ-1) in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: Park7/DJ-1 Antibody [NB300-270] - Lysates of the human RPE cell lines ARPE-19 (lane 1) and D407 (lane 2), the mouse RPE cell line B6-RPE07 (lane 3), mouse primary RPE (lane 4) and mouse brain lysates (lane 5) were harvested and analyzed by immunoblot assay with DJ-1 antibody. The DJ-1 signal varied in intensity in each RPE cell culture. Each lane contained 20 ug of protein. Protein loads were confirmed in replicate blots probed with GAPDH. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0067983), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-Western-Blot-NB300-270-img0014.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Park7/DJ-1 Antibody [NB300-270] - Representative confocal micrographs of ARPE-19 monolayers plated on Transwells(R) and labeled with antibodies to DJ-1 (G,J) and COX IV (H,K). Under baseline conditions, there is very little colocalization between DJ-1 and COX IV, as observed in overlaid images (I); DJ-1 is mostly distributed through the cytoplasm (arrows). Upon oxidative stress induced by incubation with 400 uM H2O2 for 1 hr, DJ-1 staining is increased (arrowheads) in ARPE-19 (J) cultures. In cultures treated with H2O2 some DJ-1 re-distributed to mitochondria (arrowheads) and displayed significant colocalization with COX IV in overlaid images (L). Scale bar = 10 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0067983), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-270-img0015.jpg)
![Western Blot: Park7/DJ-1 Antibody [NB300-270] - ARPE-19 and D407 monolayers were treated with increasing concentrations (0 to 800 uM) of H2O2 for 18 hrs (B), harvested, and analyzed by immunoblot assay with DJ-1 antibody (upper panel). Each lane contained 20 ug of protein. Protein loadings were confirmed in replicate blots probed with GAPDH (lower panel). A representative Western is shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0067983), licensed under a CC-BY license](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-Western-Blot-NB300-270-img0013.jpg)
![Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB300-270] - Park7(DJ-1) detected in paraffin embedded human cortex.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-Immunohistochemistry-Paraffin-NB300-270-img0007.jpg)
![Western Blot: Park7/DJ-1 Antibody [NB300-270] - Human HEK293 cells and mouse brain lysates.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-Western-Blot-NB300-270-img0005.jpg)
![Western Blot: Park7/DJ-1 Antibody [NB300-270] - Cells were transfected with the pCMV6-ENTRY control (lane 1) or pCMV6-ENTRY PARK7 cDNA (lane 2) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Park7(DJ-1).](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-Western-Blot-NB300-270-img0008.jpg)
![Western Blot: Park7/DJ-1 Antibody [NB300-270] - Presence of oxDJ-1 in RPE cells subjected to oxidative stress.ARPE-19 monolayers were treated with increasing concentrations (0 to 800 µM) of H2O2 for 1 hr (A) & 18 hs (B), harvested, & analyzed by immunoblot assay with oxDJ-1 antibody (upper panel). Protein loadings were confirmed in replicate blots probed with GAPDH (lower panel). Each lane contained 20 µg of protein. A dose response is observed when cells are exposed to increasing concentrations of H2O2 for 1 h (A, lanes 1 to 6) & 18 hrs (B, lanes 7 to 12). Confocal immunofluorescence staining of baseline ARPE-19 cultures (C) fixed before extraction with Triton X-100 & labeling with oxDJ-1 antibodies revealed absence of oxDJ-1. However, oxDJ-1 is observed in the cytoplasm (arrows) & perinuclear area (arrowheads) of RPE cells exposure to 400 µM H2O2 for 1 h (D) & 18 hrs (E). Cell nuclei were labeled with TO-PRO-3. Scale bar = 20 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23844142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-270_rabbit-polyclonal-park7-dj-1-antibody-31020241621815.jpg)
![Western Blot: Park7/DJ-1 Antibody [NB300-270] - Presence of oxDJ-1 in RPE cells subjected to oxidative stress.ARPE-19 monolayers were treated with increasing concentrations (0 to 800 µM) of H2O2 for 1 hr (A) & 18 hs (B), harvested, & analyzed by immunoblot assay with oxDJ-1 antibody (upper panel). Protein loadings were confirmed in replicate blots probed with GAPDH (lower panel). Each lane contained 20 µg of protein. A dose response is observed when cells are exposed to increasing concentrations of H2O2 for 1 h (A, lanes 1 to 6) & 18 hrs (B, lanes 7 to 12). Confocal immunofluorescence staining of baseline ARPE-19 cultures (C) fixed before extraction with Triton X-100 & labeling with oxDJ-1 antibodies revealed absence of oxDJ-1. However, oxDJ-1 is observed in the cytoplasm (arrows) & perinuclear area (arrowheads) of RPE cells exposure to 400 µM H2O2 for 1 h (D) & 18 hrs (E). Cell nuclei were labeled with TO-PRO-3. Scale bar = 20 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23844142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-270_rabbit-polyclonal-park7-dj-1-antibody-3102024162185.jpg)
![Western Blot: Park7/DJ-1 Antibody [NB300-270] - Presence of oxDJ-1 in RPE cells subjected to oxidative stress.ARPE-19 monolayers were treated with increasing concentrations (0 to 800 µM) of H2O2 for 1 hr (A) & 18 hs (B), harvested, & analyzed by immunoblot assay with oxDJ-1 antibody (upper panel). Protein loadings were confirmed in replicate blots probed with GAPDH (lower panel). Each lane contained 20 µg of protein. A dose response is observed when cells are exposed to increasing concentrations of H2O2 for 1 h (A, lanes 1 to 6) & 18 hrs (B, lanes 7 to 12). Confocal immunofluorescence staining of baseline ARPE-19 cultures (C) fixed before extraction with Triton X-100 & labeling with oxDJ-1 antibodies revealed absence of oxDJ-1. However, oxDJ-1 is observed in the cytoplasm (arrows) & perinuclear area (arrowheads) of RPE cells exposure to 400 µM H2O2 for 1 h (D) & 18 hrs (E). Cell nuclei were labeled with TO-PRO-3. Scale bar = 20 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23844142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-270_rabbit-polyclonal-park7-dj-1-antibody-310202416212344.jpg)
![Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Total protein from human HepG2 and HeLa cells, rat PC12 cells and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Beta catenin in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Western-Blot-NBP1-54467-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Total protein from human HepG2 and HeLa cells, rat PC12 cells and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Beta catenin in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation. </b>Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Activity of Wnt/beta-catenin pathway in the embryonic chick lung. Western blot analysis of active and total beta-catenin in stage b1, b2 and b3 lungs, and stage 24 limb (as positive control). Control loading was performed using beta-tubulin (55 kDa). Total and active beta-catenin correspond to 92 kDa. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0112388), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Western-Blot-NBP1-54467-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (12F7) [NBP1-54467] - The beta- Catenin antibody was tested in MCF-7 cells against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 550 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Immunocytochemistry-Immunofluorescence-NBP1-54467-img0004.jpg)
![Immunohistochemistry: beta-Catenin Antibody (12F7) [NBP1-54467] - Beta-catening (green) was detected in human skin (psoriasis) using beta-catenin-FITC antibody (1:40) in PBS for 1 hour. Nuclei were stained with Dapi (blue). Image from verified customer review. Image using the FITC format of this antibody.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Immunohistochemistry-NBP1-54467-img0013.jpg)
![Flow Cytometry: beta-Catenin Antibody (12F7) [NBP1-54467] - An intracellular stain was performed on SK-MEL-28 cells with beta-Catenin Antibody [12F7] NBP1-54467B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Flow-Cytometry-NBP1-54467-img0012.jpg)
![Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Analysis of beta Catenin expression in 1) HepG2, 2) MCF7, and 3) Cos7 whole cell lysates using NBP1-54467.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Western-Blot-NBP1-54467-img0001.jpg)
![Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Analysis of beta- Catenin in embryonic lung (lane 1) and embryonic limb (lane 2) lysates using anti-beta- Catenin antibody. Each lane was loaded with 5ug of protein sample. Image from verified customer review.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Western-Blot-NBP1-54467-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (12F7) [NBP1-54467] - Analysis of beta Catenin in mouse intestine using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Immunohistochemistry-Paraffin-NBP1-54467-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (12F7) [NBP1-54467] - Analysis of beta- Catenin in mouse intestine using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Immunohistochemistry-Paraffin-NBP1-54467-img0003.jpg)
![Flow (Intracellular): beta-Catenin Antibody (12F7) [NBP1-54467] - An intracellular stain was performed on HeLa cells with beta-Catenin Antibody (12F7) NBP1-54467AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Flow-Intracellular-NBP1-54467-img0009.jpg)
![Simple Western: beta-Catenin Antibody (12F7) [NBP1-54467] - Simple Western lane view shows a specific band for Beta- Catenin in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-12F7-Simple-Western-NBP1-54467-img0006.jpg)
![Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201] - Immunofluorescence photomicrograph showing that the expression of Neurokinin B in the arcuate nucleus (ARC). Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32921318/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody---BSA-Free-Immunohistochemistry-NB300-201-img0009.jpg "<b>Biological Strategies Validation. </b>Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201] - Immunofluorescence photomicrograph showing that the expression of Neurokinin B in the arcuate nucleus (ARC). Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32921318/) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:200 dilution. The antibody depicted an expected strong cytoplasmic staining in the neuronal cells.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody-Immunohistochemistry-Paraffin-NB300-201-img0005.jpg)
![Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:100 dilution. The antibody showed a specific cytoplasmic staining in the Purkinje cells and some granular neurons from the granular layer with weak signal in the fibers within the molecular layer of the section.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody-Immunohistochemistry-Paraffin-NB300-201-img0006.jpg)
![Immunohistochemistry: Neurokinin B Antibody [NB300-201] - IF analysis of Neurokinin B in mouse hypothalamus. Image courtesy of an anonymous customer review.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody-Immunohistochemistry-NB300-201-img0007.jpg)
![Immunohistochemistry: Neurokinin B Antibody [NB300-201] - Detection of Neurokinin B in rat brain tissue (Alexa 488 stain), using NB 300-201.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody-Immunohistochemistry-NB300-201-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: Neurokinin B Antibody - BSA Free [NB300-201] - Troxerutin reduced the expression of Kisspepetin1 & Neurokinin B in the ARC. a Immunofluorescence photomicrograph showing that the expression of Kisspeptin1 & NKB in the arcuate nucleus (ARC). Scale bars:20 μm. b A lower magnification image showing the whole ARC & median eminence. Rectangular frame a & b represent ARC & median eminence (ME) analyzed in Fig. 5a & Fig. 6a, respectively. Scale bars:200 μm. c Quantitative analysis of integral optical density (IOD) of Kisspeptin1 & NKB in the ARC. P values were determined by one-way ANOVA with Tukey’s multiple comparison test & data were presented as means±s.e.m. n = 4 per group. **P < 0.01, ***P < 0.001vs. sham group; #P < 0.05, ##P < 0.01 vs. PCOS group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32921318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-201_rabbit-polyclonal-neurokinin-b-antibody-310202415175279.jpg)
![Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201] - Characterization of Kiss1-null mice.(A-C) Immunofluorescence for kisspeptin (A,B) & NKB (C) in the ARN of wild-type (A) & Kiss1-null (B,C) female mice. (D) Cell-attached recordings showing the effects of 100 nM NKB on firing of KNDy neurons in acute brain slices prepared from female heterozygous Kiss1Cre/+ and homozygous (null) Kiss1Cre/Cre;Rosa26-tdT mice. (E) Mean ± SEM changes in KNDy neuron firing evoked by 100 nM NKB in heterozygous Kiss1Cre/+and homozygous (null) Kiss1Cre/Cre;Rosa26-tdT mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33464205), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-201_rabbit-polyclonal-neurokinin-b-antibody-310202415291957.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody (GA-5) [NBP2-29415] - Cultured Rat Hippocampal Neurons. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GFAP-Antibody-GA-5-Immunocytochemistry-Immunofluorescence-NBP2-29415-img0007.jpg "Immunocytochemistry/Immunofluorescence: GFAP Antibody (GA-5) [NBP2-29415] - Cultured Rat Hippocampal Neurons. ICC/IF image submitted by a verified customer review.")
![Western Blot: GFAP Antibody (GA-5) [NBP2-29415] - Western Blot Analysis of human brain tissue lysate using GFAP Antibody (GA-5).](http://images.novusbio.com/fullsize/GFAP-Antibody-GA-5-Western-Blot-NBP2-29415-img0011.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody (GA-5) [NBP2-29415] - Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Antibody (GA-5).](http://images.novusbio.com/fullsize/GFAP-Antibody-GA-5-Immunohistochemistry-Paraffin-NBP2-29415-img0010.jpg)
![Flow Cytometry: GFAP Antibody (GA-5) [NBP2-29415] - Flow Cytometric Analysis of T98G cells using GFAP Antibody (GA-5) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/GFAP-Antibody-GA-5-Flow-Cytometry-NBP2-29415-img0009.jpg)
![Western Blot: GFAP Antibody (GA-5) [NBP2-29415] - Analysis of GFAP in human brain lysate using GFAP (GA5) antibody at 1 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/GFAP-Antibody-GA-5-Western-Blot-NBP2-29415-img0001.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody (GA-5) [NBP2-29415] - Formalin-paraffin human brain stained with GFAP Ab (GA-5). Note cytoplasmic staining.](http://images.novusbio.com/fullsize/GFAP-Antibody-GA-5-Immunohistochemistry-Paraffin-NBP2-29415-img0003.jpg)
![Flow Cytometry: GFAP Antibody (GA-5) [NBP2-29415] - Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and mononuclear cells were isolated from the CNS at day 10 (onset of symptoms). Cells were stained for GFAP, Neun, CX3CL1, CXCL12, CCL2, CD45 and CD11b, plus for viability to exclude dead cells. GFAP staining is shown for viable cells. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GFAP-Antibody-GA-5-Flow-Cytometry-NBP2-29415-img0008.jpg)
![Simple Western: GFAP Antibody (GA-5) [NBP2-29415] - Simple Western lane view shows a specific band for GFAP in 0.2 mg/mL of human brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GFAP-Antibody-GA-5-Simple-Western-NBP2-29415-img0004.jpg)
![Simple Western: GFAP Antibody (GA-5) [NBP2-29415] - Electropherogram image of the corresponding Simple Western lane. GFAP antibody was used at 10 ug/mL dilution of human brain lysate.](http://images.novusbio.com/fullsize/GFAP-Antibody-GA-5-Simple-Western-NBP2-29415-img0006.jpg)
![Immunohistochemistry-Paraffin: Mouse Monoclonal GFAP Antibody (GA-5) [NBP2-29415] - Analysis of GFAP on adult human brain tissue. Antigen retrieval in a basic buffer x200 (1ug/mL). Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-29415_mouse-monoclonal-gfap-antibody-ga-5-immunohistochemistry-paraffin-3102023141238..jpg)
![Immunocytochemistry/ Immunofluorescence: GFAP Antibody (GA-5) [NBP2-29415] - Advancing super‐resolution microscopy for astroglial research. (A) STED images showing CA1 stratum radiatum astrocytic processes (green, whole‐cell loading with Alexa Fluor 488) adjacent to synaptic structures in organotypic slices (red; Thy1‐YFP; dendritic spines, arrows) at lower (A1) & higher (A2, area indicted by square in A1) magnification; asterisk, O‐ring structures indicating tentative cell process envelopes; adapted from (Panatier et al., 2014). (B) STED image of P2Y1 receptors (red) along a multi‐branched astrocytic process (glutamine synthase, grey) in the adult mouse hippocampus. Adapted from (Volterra et al., 2014). (C) STORM imaging of pre‐ (Bassoon; red) & postsynaptic (Homer1; green) scaffolding proteins in the mouse main olfactory bulb glomeruli imaged using conventional fluorescence imaging (C1) & STORM (C2); adapted from (Dani et al., 2010). (D, E) dSTORM images of cultured (14 DIV) mixed glial cells from rat hippocampus (ProLong Diamond in Zeiss Elyra PS.1 microscope; Fiji Plugin ThunderSTORM, 3,000 frames); unpublished data by J. Heller. (D) GFAP stained with monoclonal antibody (Novus, GA5, secondary Alexa Fluor 488 donkey anti‐mouse antibody, Life Technologies) shown at lower (D1) & higher (D2, fragment indicated in D1) magnification. (E) GLT‐1 (polyclonal, Millipore) visualized with Alexa Fluor 568 goat anti‐guinea pig antibody (Life Technologies) shown at lower (E1) & higher (E2, fragment indicated in E1) magnification. Note that the cells were permeabilized, & therefore GLT‐1 was stained throughout cellular compartments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25782611), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-29415_mouse-monoclonal-gfap-antibody-ga-5-imgenex-nbo-107a-20ug-310202416114755.jpg)
