| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-266SS | |||
| Azide and BSA Free | NBP2-80869 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92717SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-39100-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89468-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72627 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61745-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83349-25ul | |||
![Immunohistochemistry: Nestin Antibody (10C2) [NB300-266] - GFAP and Nestin immunoreactivity in retinal sections.Epifluorescent acquisition of p28 Reeler and WT retinas. (A-D) As compared to WT, both GFAP (red) and Nestin (green) immunoreactivities were highly visible in Reeler retinas (AB, GCL). Arrows point at yellow immunoreactivity in the white frame (B) indicating GFAP and Nestin co-expression in cells having long-filaments (activated Muller cells), as compared to wild type (arrowheads in A). Nuclei were DAPI counterstained (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212732) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Immunohistochemistry-NB300-266-img0020.jpg "Immunohistochemistry: Nestin Antibody (10C2) [NB300-266] - GFAP and Nestin immunoreactivity in retinal sections.Epifluorescent acquisition of p28 Reeler and WT retinas. (A-D) As compared to WT, both GFAP (red) and Nestin (green) immunoreactivities were highly visible in Reeler retinas (AB, GCL). Arrows point at yellow immunoreactivity in the white frame (B) indicating GFAP and Nestin co-expression in cells having long-filaments (activated Muller cells), as compared to wild type (arrowheads in A). Nuclei were DAPI counterstained (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212732) licensed under a CC-BY license.")
![Western Blot: Nestin Antibody (10C2) [NB300-266] - WB analysis of 5 ug lysate from (1) Rat's total brain tissue, and (2) human CNS progenitor cells using Nestin antibody (clone 10C2) at 1:1000 dilution. The antibody did not react with rat, whereas, in lysate from human samples, it generated a doublet band representing the phosphorylated and the non-phosphorylated forms of Nestin protein.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Western-Blot-NB300-266-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (10C2) [NB300-266] - Analysis of formalin fixed, 0.1% Triton X-100 permeabilized F9 cells (Mouse teratocarcinoma) using Nestin antibody (clone 10C2) at 1:50 dilution. The primary antibody was detected using Alexa Fluor 488 labeled donkey anti-mouse IgG (H+L) cross-adsorbed secondary antibody (green) and nuclei were counterstained with DAPI (blue). An isotype control set was inclubated with mouse IgG1 isotype control antibody and the above mentioned secondary antibody. Nestin antibody (clone 10C2) generated a specific membrane-cytoplasmic staining of Nestin while the isotype control samples did not show any staining under same assay conditions.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Immunocytochemistry-Immunofluorescence-NB300-266-img0015.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody (10C2) [NB300-266] - Nestin was detected in immersion fixed paraffin-embedded sections of human kidney using anti-human mouse monoclonal antibody (Catalog # NB300-266) at 1:500 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Immunohistochemistry-Paraffin-NB300-266-img0013.jpg)
![Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Nestin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1 x 10^6 cells was used.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Flow-Cytometry-NB300-266-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (10C2) [NB300-266] - Visualization of Nestin in PC-3 cells using NB300-266. Courtesy of Dolnick Lab, Roswell Park Cancer Institute.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Immunocytochemistry-Immunofluorescence-NB300-266-img0006.jpg)
![Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - An intracellular stain was performed on SH-SY5Y cells with Nestin [10C2] Antibody NB300-266C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Flow-Cytometry-NB300-266-img0017.jpg)
![Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - An intracellular stain was performed on HeLa cells with Nestin Antibody (10C2)NB300-266PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Flow-Cytometry-NB300-266-img0016.jpg)
![Immunohistochemistry: Nestin Antibody (10C2) - BSA Free [NB300-266] - Regorafenib inhibits tumour growth and vascularisation in xenograft mice. GC1 were implanted into the right forebrain of mice. The mice then received daily oral vehicle or regorafenib at 30 mg/kg for 45 days. Representative photographs of mice brain tumours treated with regorafenib (right panel) or without regorafenib (left panel) stained by hemalun-eosin (median panel) or with nestin antibody (upper and lower panel). The upper panel shows whole brains of mice stained with nestin antibody. The tumour areas were circled in blue, and each surface was measured in mm^2. The red rectangles indicate the zone of the tumour shown in median (hemalun-eosin staining) and lower (nestin IHC) panel. Scale bars, upper panel 2.5 mm, median and lower panel 100 um. The graph shows the tumour size in mm^2 (expressed as the mean +/- SEM of 4 mice). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35326702/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb300-266_mouse-monoclonal-nestin-antibody-10c2-20920231412574.jpg)
![Immunohistochemistry: Nestin Antibody (10C2) - BSA Free [NB300-266] - Regorafenib inhibits tumour growth & vascularisation in xenograft mice. GC1 were implanted into the right forebrain of mice. The mice then received daily oral vehicle or regorafenib at 30 mg/kg for 45 days. (A) Representative photographs of mice brain tumours treated with regorafenib (right panel) or without regorafenib (left panel) stained by hemalun–eosin (median panel) or with nestin antibody (upper & lower panel). The upper panel shows whole brains of mice stained with nestin antibody. The tumour areas were circled in blue, & each surface was measured in mm2. The red rectangles indicate the zone of the tumour shown in median (hemalun–eosin staining) & lower (nestin IHC) panel. Scale bars, upper panel 2.5 mm, median & lower panel 100 µm. The graph shows the tumour size in mm2 (expressed as the mean ± SEM of 4 mice). (B) Representative immunohistochemistry photographs of CD31+ vessels in brain tumour areas of 3 different mice treated with regorafenib (right panel) or without regorafenib (control, left panel). Arrows indicate CD31+ blood vessels. Scale bars, 20 µm. The graph shows the number of CD31+ vessels in tumours per mm2 (expressed as mean ± SEM of 4 mice). * p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35326702), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-266_mouse-monoclonal-nestin-antibody-10c2-310202416212379.jpg)
![Western Blot: Nestin Antibody (4D11) [NBP1-92717] - Analysis analysis of tissue and cell lysates with mouse mAB against nestin, NBP1-92717, dilution 1:500 in green: [1] protein standard, [2] embryonic E18 rat brain, [3] C6 rat glioma cells, and [4] SH-SY5Y human neuroblastoma cells.](http://images.novusbio.com/fullsize/Nestin-Antibody-4D11-Western-Blot-NBP1-92717-img0006.jpg "Western Blot: Nestin Antibody (4D11) [NBP1-92717] - Analysis analysis of tissue and cell lysates with mouse mAB against nestin, NBP1-92717, dilution 1:500 in green: [1] protein standard, [2] embryonic E18 rat brain, [3] C6 rat glioma cells, and [4] SH-SY5Y human neuroblastoma cells.")
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (4D11) [NBP1-92717] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to nestin, NBP1-92717, dilution 1:500 in red, and costained with chicken pAb to MAP2, dilution 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.](http://images.novusbio.com/fullsize/Nestin-Antibody-4D11-Immunocytochemistry-Immunofluorescence-NBP1-92717-img0005.jpg)
![Immunohistochemistry: Nestin Antibody (4D11) [NBP1-92717] - Staining in mouse cortex microslice section (from postnatal day 3). IHC image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Nestin-Antibody-4D11-Immunohistochemistry-NBP1-92717-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (4D11) [NBP1-92717] - Mixed cultures of neonatal rat neurons and glia stained with NBP1-92717 (red), chicken antibody to Vimentin (NB300-223, green) and DNA (DAPI stain, blue). Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the Nestin (4D11) antibody. The filamentous staining pattern is as expected as both Nestin and Vimentin are components of 10nm filaments. Note that some cells contain Nestin, but do not stain strongly for Vimentin and so appear red. Others contain Vimentin and not Nestin and so appear green - these are likely to be fibroblastic or endothelial cells. Some cells express both proteins and so appear yellowish.](http://images.novusbio.com/fullsize/Nestin-Antibody-4D11-Immunocytochemistry-Immunofluorescence-NBP1-92717-img0004.jpg)
![Western Blot: USP7 Antibody [NB100-513] - Samples: Wholecell lysate (10 ug) from HEK293T, RKO, Hep-G2, Jurkat,HeLa, TCMK-1, and NIH 3T3 cells prepared using NETNlysis buffer. Antibody: Affinity purified rabbit anti-USP7antibody used for WB at0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 10 seconds](http://images.novusbio.com/fullsize/USP7-Antibody-Western-Blot-NB100-513-img0004.jpg "Western Blot: USP7 Antibody [NB100-513] - Samples: Wholecell lysate (10 ug) from HEK293T, RKO, Hep-G2, Jurkat,HeLa, TCMK-1, and NIH 3T3 cells prepared using NETNlysis buffer. Antibody: Affinity purified rabbit anti-USP7antibody used for WB at0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 10 seconds")
![Immunohistochemistry-Paraffin: USP7 Antibody [NB100-513] - Sample: FFPE section of human ovarian carcinoma.Antibody: Affinity purified rabbit anti-USP7 used at 1:5,000 (0.2ug/ml). Detection:DAB](http://images.novusbio.com/fullsize/USP7-Antibody-Immunohistochemistry-Paraffin-NB100-513-img0002.jpg)
![Immunohistochemistry-Paraffin: USP7 Antibody [NB100-513] - Sample: FFPE section of mouse renal cell carcinoma.Antibody: Affinity purified rabbit anti-USP7 used at 1:5,000 (0.2ug/ml). Detection:DAB](http://images.novusbio.com/fullsize/USP7-Antibody-Immunohistochemistry-Paraffin-NB100-513-img0001.jpg)
![Immunoprecipitation: USP7 Antibody [NB100-513] - Samples: Wholecell lysate (10 ug) from HEK293T, RKO, Hep-G2, Jurkat,HeLa, TCMK-1, and NIH 3T3 cells prepared using NETNlysis buffer. Antibody: Affinity purified rabbit anti-USP7antibody used for WB at0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 10 seconds](http://images.novusbio.com/fullsize/USP7-Antibody-Immunoprecipitation-NB100-513-img0003.jpg)
![Western Blot: Synaptopodin Antibody [NBP2-39100] - Analysis of SYNPO in rat brain tissue lysate with SYNPO antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/Synaptopodin-Antibody-Western-Blot-NBP2-39100-img0012.jpg "Western Blot: Synaptopodin Antibody [NBP2-39100] - Analysis of SYNPO in rat brain tissue lysate with SYNPO antibody at (A) 1 and (B) 2 ug/mL.")
![Immunohistochemistry: Synaptopodin Antibody [NBP2-39100] - Mouse brain tissue with SYNPO antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Synaptopodin-Antibody-Immunohistochemistry-NBP2-39100-img0015.jpg)
![Western Blot: Synaptopodin Antibody - BSA Free [NBP2-39100] - mTOR signaling pathway regulated PKM2 expression & podocyte differentiation.a IHC detection of p-S6 in renal cortex during postnatal development. Scale bar=20 μm. b Representative western immunoblot analysis of p-S6 in renal cortex. c Representative western immunoblot analysis of p-S6 in cultured podocyte during differentiation. d Western immunoblot analysis of p-S6 & PKM2 expression in Tsc1fl/fl & Tsc1−/−-podocytes. e Rapamycin caused a significant reduction in the protein levels of p-S6 & PKM2 in cultured podocyte. f Western immunoblot analysis of nephrin & synaptopodin with 10 nM rapamycin treatment for 14 days. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32393782), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-39100_rabbit-polyclonal-synaptopodin-antibody-310202415293341.jpg)
![Western Blot: Synaptopodin Antibody - BSA Free [NBP2-39100] - Metabolomics analysis revealed higher lactate production in differentiated podocytes.a Immunofluorescence staining for nephrin (red), phalloidine for F-actin (green) & DAPI for nuclear (blue) in undifferentiated podocytes (UDPs) or differentiated podocytes (DPs) as indicated (n = 5). Scale bar=5 μm. b Representative western blotting results of nephrin, podocin & synaptopodin confirm the differentiation of podocytes (n = 3). c Representative map of 1H-NMR spectra in the extracellular medium incubated with the presence of different podocytes (n = 6). UDM: undifferentiated podocyte medium; DM: differentiated podocyte medium. The map shows the significance of metabolites variations between these two classes. Peaks in the positive direction indicate metabolites that are more abundant in the UDM groups. Consequently, metabolites that are more abundant in DM are presented as peaks in the negative direction. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32393782), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-39100_rabbit-polyclonal-synaptopodin-antibody-310202416113510.jpg)
![Immunocytochemistry/ Immunofluorescence: Synaptopodin Antibody - BSA Free [NBP2-39100] - Immunofluorescence of Synaptopodin in human brain tissue with Synaptopodin antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-39100_rabbit-polyclonal-synaptopodin-antibody-2711202419251591.jpg)
![Immunohistochemistry: Synaptopodin Antibody - BSA Free [NBP2-39100] - Immunohistochemistry of Synaptopodin in human brain tissue with Synaptopodin antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp2-39100_rabbit-polyclonal-synaptopodin-antibody-2711202420405589.jpg)
![Immunocytochemistry/ Immunofluorescence: Synaptopodin Antibody - BSA Free [NBP2-39100] - Immunofluorescence of Synaptopodin in mouse brain tissue with Synaptopodin antibody at 20 u/mL.Red: Synaptopodin Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-39100_rabbit-polyclonal-synaptopodin-antibody-271120242092058.gif)
![Western Blot: Staufen Antibody [NBP1-33202] - Western blot analysis of LBCs show increased Staufen (STAU1) (NBP1-33202) levels compared with normal controls. DDX6 (NB200-191) levels are unchanged. HD and SCA3 patient (polyQ expanded) FBs were used as additional controls. Four normal and five SCA2 FBs, and two normal and three SCA2 LBCs were used.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194296/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP1-33202-img0011.jpg "Western Blot: Staufen Antibody [NBP1-33202] - Western blot analysis of LBCs show increased Staufen (STAU1) (NBP1-33202) levels compared with normal controls. DDX6 (NB200-191) levels are unchanged. HD and SCA3 patient (polyQ expanded) FBs were used as additional controls. Four normal and five SCA2 FBs, and two normal and three SCA2 LBCs were used.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194296/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Staufen Antibody [NBP1-33202] - Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Staufen stained by Staufen antibody diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Staufen-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33202-img0008.jpg)
![Western Blot: Staufen Antibody [NBP1-33202] - Sample (30 ug of whole cell lysate) A: Neuro2A diluted at 1:1000](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP1-33202-img0005.jpg)
![Western Blot: Staufen Antibody [NBP1-33202] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Staufen antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP1-33202-img0010.jpg)
![Immunoprecipitation: Staufen Antibody [NBP1-33202] - Staufen antibody staining 30 ug whole cell lysate of Lane A: 293 (input), Lane B: control rabbit IgG-IP, Lane C: Stau1-IP, Lane D: Post-IP lysate from control rabbit IgG-IP, Lane E:Post-IP lysate from Stau1-IP; diluted at 1:3000.](http://images.novusbio.com/fullsize/Staufen-Antibody-Immunoprecipitation-NBP1-33202-img0006.jpg)
![Western Blot: Staufen Antibody [NBP1-33202] - Silencing of STAU1 mitigates SCA2 phenotypes. aStau1 haploinsufficiency improves abnormal motor behavior of ATXN2Q127 mice as determined by rotarod behavior at 8, 12, 16, & 20 weeks of age. ATXN2Q127;Stau1+/- mice (green) have improved rotarod performance compared with ATXN2Q127 littermates (red) starting at 12 weeks of age. Note that Stau1 haploinsufficiency (orange) by itself does not alter motor function; n = 9–15 mice per group. Values shown are mean ± SE. Significance was determined using generalized estimating equations (GEE). NS, nonsignificant, *P 4 pixels per cell positive for both ATXN2 & STAU1 are shown. One-hundred normal & 96 SCA2-FBs were used for analyses. Data are mean ± SD, **P 4 pixels per cell positive for both ATXN2 & STAU1 are shown. One-hundred normal & 96 SCA2-FBs were used for analyses. Data are mean ± SD, **P 4 pixels per cell positive for both ATXN2 & STAU1 are shown. One-hundred normal & 96 SCA2-FBs were used for analyses. Data are mean ± SD, **P Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Staufen stained by Staufen antibody (NBP1-33202) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-33202_rabbit-polyclonal-staufen-antibody-immunocytochemistry-immunofluorescence-26112025185041.jpg)
![Immunocytochemistry/Immunofluorescence: MEF2C Antibody [NBP1-89468] - Staining of human cell line RH-30 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MEF2C-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89468-img0003.jpg "Immunocytochemistry/Immunofluorescence: MEF2C Antibody [NBP1-89468] - Staining of human cell line RH-30 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.")
![Western Blot: MEF2C Antibody (1H5) [NBP2-00493] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEF2C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEF2C.](http://images.novusbio.com/fullsize/MEF2C-Antibody-1H5-Western-Blot-NBP2-00493-img0004.jpg "Western Blot: MEF2C Antibody (1H5) [NBP2-00493] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEF2C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEF2C.")
![Immunocytochemistry/Immunofluorescence: MEF2C Antibody (OTI1H5) [NBP2-00493] - MEF2C Antibody (1H5) [NBP2-00493] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEF2C.](http://images.novusbio.com/fullsize/MEF2C-Antibody-1H5-Immunocytochemistry-Immunofluorescence-NBP2-00493-img0010.jpg)
![Immunohistochemistry-Paraffin: MEF2C Antibody (1H5) [NBP2-00493] - Staining of paraffin-embedded Human Ovary tissue using anti-MEF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MEF2C-Antibody-1H5-Immunohistochemistry-Paraffin-NBP2-00493-img0008.jpg)
![Flow Cytometry: MEF2C Antibody (1H5) [NBP2-00493] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MEF2C antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/MEF2C-Antibody-1H5-Flow-Cytometry-NBP2-00493-img0003.jpg)
![Immunohistochemistry-Paraffin: MEF2C Antibody (1H5) [NBP2-00493] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MEF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MEF2C-Antibody-1H5-Immunohistochemistry-Paraffin-NBP2-00493-img0005.jpg)
![Immunohistochemistry-Paraffin: MEF2C Antibody (1H5) [NBP2-00493] - Staining of paraffin-embedded Human lymph node tissue using anti-MEF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MEF2C-Antibody-1H5-Immunohistochemistry-Paraffin-NBP2-00493-img0006.jpg)
![Immunohistochemistry-Paraffin: MEF2C Antibody (1H5) [NBP2-00493] - Staining of paraffin-embedded Human lymphoma tissue using anti-MEF2C mouse monoclonal antibody.](http://images.novusbio.com/fullsize/MEF2C-Antibody-1H5-Immunohistochemistry-Paraffin-NBP2-00493-img0007.jpg)
![Flow Cytometry: MEF2C Antibody (1H5) [NBP2-00493] - Analysis of Hela cells, using anti-MEF2C antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/MEF2C-Antibody-1H5-Flow-Cytometry-NBP2-00493-img0001.jpg)
![Flow Cytometry: MEF2C Antibody (1H5) [NBP2-00493] - Analysis of Jurkat cells, using anti-MEF2C antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/MEF2C-Antibody-1H5-Flow-Cytometry-NBP2-00493-img0002.jpg)
![Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mAb against HEK293 (1) and DDX1 (AA: 642-740)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Western-Blot-NBP2-61745-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of Hela cells using DDX1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Immunocytochemistry-Immunofluorescence-NBP2-61745-img0003.jpg)
![Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of bladder cancer tissues using DDX1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Immunohistochemistry-NBP2-61745-img0005.jpg)
![Flow Cytometry: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of Hela cells using DDX1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Flow-Cytometry-NBP2-61745-img0002.jpg)
![Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mAb against human DDX1 (AA: 642-740) recombinant protein. (Expected MW is 37 kDa)](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Western-Blot-NBP2-61745-img0006.jpg)
![Western Blot: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis using DDX1 mouse mAb against Hela (1), MCF-7 (2), A431 (3), PC-3 (4), NIH/3T3 (5), Jurkat (6), U251 (7), and HEK293 (8) cell lysate.](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Western-Blot-NBP2-61745-img0008.jpg)
![Immunohistochemistry-Paraffin: DDX1 Antibody (3E5E2) [NBP2-61745] - Analysis of colon cancer tissues using DDX1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-Immunohistochemistry-NBP2-61745-img0004.jpg)
![ELISA: DDX1 Antibody (3E5E2) [NBP2-61745] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/DDX1-Antibody-3E5E2-ELISA-NBP2-61745-img0001.jpg)
![Immunohistochemistry-Paraffin: MEF2C Antibody [NBP2-17260] - Mouse brain. MEF2C stained by MEF2C antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MEF2C-Antibody-Immunohistochemistry-Paraffin-NBP2-17260-img0018.jpg "Immunohistochemistry-Paraffin: MEF2C Antibody [NBP2-17260] - Mouse brain. MEF2C stained by MEF2C antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: MEF2C Antibody [NBP2-17260] - Mouse fore brain. MEF2C antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MEF2C-Antibody-Immunohistochemistry-NBP2-17260-img0005.jpg)
![Immunohistochemistry-Paraffin: MEF2C Antibody [NBP2-17260] - Saos2 xenograft. MEF2C antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MEF2C-Antibody-Immunohistochemistry-Paraffin-NBP2-17260-img0007.jpg)
![Western Blot: MEF2C Antibody [NBP2-17260] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MEF2C antibody (NBP2-17260) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17260_rabbit-polyclonal-mef2c-antibody-910202420131220.jpg)
![MEF2C antibody immunoprecipitates MEF2C protein in IP experiments.IP samples: K562 whole cell extractA. Control with 4 ug of preimmune Rabbit IgGB. Immunoprecipitation of MEF2C protein by 4 ug MEF2C antibody (NBP2-17260)5 % SDS-PAGEThe immunoprecipitated MEF2C protein was detected by MEF2C antibody (NBP2-17260) diluted at 1:1000.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-17260_rabbit-polyclonal-mef2c-antibody-immunoprecipitation-261120251821333.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. Western blot analysis of p53 and MDM2/HDM2 in wt MEFs treated with 10 uM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). Anti-beta-actin (NB600-501 1:20 000) Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-17376-0) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Western-Blot-NBP2-17247-img0027.jpg "<b>Biological Strategies Validation. </b>Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. Western blot analysis of p53 and MDM2/HDM2 in wt MEFs treated with 10 uM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). Anti-beta-actin (NB600-501 1:20 000) Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-17376-0) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer. MDM2 stained by MDM2 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0028.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - Human lung cancer, using MDM2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-Immunohistochemistry-Paraffin-NBP2-17247-img0003.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Lack of HIF-P4H-1 increases the amount of p53. (a) Western blot analysis of p53 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (b,c) Western blot analysis of p53 in Hif-p4h-1 & scrambled (Scrm) siRNA transfected HEK293 cells (b) & wt MEFs treated with 50 µM FG4497 for 24 h (c). (d) qPCR analysis of p53 mRNA in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (e) Analysis of p53 protein turnover rate. Hif-p4h-1−/− & wt MEFs were treated with 200 μg/ml of cycloheximide for the indicated time points & whole cell lysates were blotted for p53. (f) Western blot analysis of MDM2 in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h. (g) Western blot analysis of p53 & MDM2 in wt MEFs treated with 10 μM nutlin-3a for 24 h with or without overexpression of human HIF-P4H-1 (OE). (h,i) Western blot analysis of HIF1 alpha in wt & Hif-p4h-1−/− MEFs cultured in 21% or 1% O2 for 24 h (h) & in scrambled & Hif-p4h-1 siRNA transfected HEK293 cells (i). (j) Western blot analysis of ubiquitination of p53 in Hif-p4h-1 & scrambled siRNA transfected HEK293 cells. The cells were transfected with HA-ubiquitin & endogenous p53 was immunoprecipitated followed by Western blotting with anti-HA & anti-P53 antibodies. (k) Western blot analysis of p53 in Hif-p4h-1−/− & wt MEFs were treated with or without 10 μM MG132 for 5 h. Data are presented as representative Western blots & as mean ± s.d., n = at least 3 individual MEF isolates or experiments. *P Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MDM2/HDM2 stained by MDM2/HDM2 antibody (NBP2-17247) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091472.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242010445.jpg)
![Western Blot: MDM2/HDM2 Antibody [NBP2-17247] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2/HDM2 antibody (NBP2-17247) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-91020242091496.jpg)
![Immunohistochemistry-Paraffin: MDM2/HDM2 Antibody [NBP2-17247] - MDM2/HDM2 antibody detects MDM2/HDM2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.MDM2/HDM2 stained by MDM2/HDM2 antibody (NBP2-17247) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-910202420134015.jpg)
![MDM2 antibody immunoprecipitates MDM2 protein in IP experiments.IP samples: Jurkat whole cell extractA. 30 ug Jurkat cell whole cell extractB. Control with 4 ug of preimmune Rabbit IgGC. Immunoprecipitation of MDM2 protein by 4 ug MDM2 antibody (NBP2-17247)7.5 % SDS-PAGEThe immunoprecipitated MDM2 protein was detected by MDM2 antibody (NBP2-17247) diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-17247_rabbit-polyclonal-mdm2-hdm2-antibody-immunoprecipitation-261120251826130.jpg)
![Western Blot: AHNAK Antibody (3G7) [H00079026-M01] - Analysis of AHNAK expression in transfected 293T cell line by AHNAK monoclonal antibody (M01), clone 3G7.Lane 1: AHNAK transfected lysate(16 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/AHNAK-Antibody-3G7-Western-Blot-H00079026-M01-img0006.jpg "Western Blot: AHNAK Antibody (3G7) [H00079026-M01] - Analysis of AHNAK expression in transfected 293T cell line by AHNAK monoclonal antibody (M01), clone 3G7.Lane 1: AHNAK transfected lysate(16 KDa).Lane 2: Non-transfected lysate.")
![Immunoprecipitation: AHNAK Antibody (3G7) [H00079026-M01] - Transfected lysate using anti-AHNAK monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with AHNAK MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/AHNAK-Antibody-3G7-Immunoprecipitation-H00079026-M01-img0003.jpg)
![Immunoprecipitation: AHNAK Antibody (3G7) [H00079026-M01] - Analysis of AHNAK transfected lysate using anti-AHNAK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AHNAK MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/AHNAK-Antibody-3G7-Immunoprecipitation-H00079026-M01-img0004.jpg)
![ELISA: AHNAK Antibody (3G7) [H00079026-M01] - Detection limit for recombinant GST tagged AHNAK is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/AHNAK-Antibody-3G7-ELISA-H00079026-M01-img0007.jpg)
![Western Blot: LRPPRC Antibody [NBP1-83349] - Analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LRPPRC antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Western-Blot-NBP1-83349-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LRPPRC Antibody [NBP1-83349] - Analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LRPPRC antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LRPPRC Antibody [NBP1-83349] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83349-img0007.jpg)
