| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1596SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47470SS | |||
| Azide and BSA Free | NBP2-80757 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-78644SS | |||
| Azide and BSA Free | NBP2-80781 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-81946-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85368-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92879-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92759-0.02ml | |||
![Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Western-Blot-NB100-1596-img0005.jpg "Western Blot: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) and hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Aromatase Antibody [NB100-1596] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aromatase Antibody NB100-1596 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1596-img0008.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control rabbits. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control ovaries. Nonlabeling was observed when the primary antibody was omitted. Bars: 200 um (1st image) and 20 um (2nd image). Granulose cells, gc; theca cells, tc. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NB100-1596-img0006.jpg)
![Western Blot: Aromatase Antibody [NB100-1596] - Detection of aromatase in human fetal temporal lobe lysate using NB 100-1596. ECL exposure, 1-2 minutes.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Western-Blot-NB100-1596-img0003.jpg)
![Immunohistochemistry-Paraffin: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of hypothyroid (Hypo; solid bar; n = 6) rabbits. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of hypothyroid ovaries. Nonlabeling was observed when the primary antibody was omitted. Bars: 100 um (1st image); and 20 um (2nd image). Granulose cells, gc; theca cells, tc. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2017/3795950/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase-Antibody-Immunohistochemistry-Paraffin-NB100-1596-img0007.jpg)
![Simple Western: Aromatase Antibody [NB100-1596] - Aromatase expression by Western blotting (WES system) from in vitro activated M1 and M2 rat macrophages using Aromatase antibody. Controls include ovary (positive control) and ovary in which the primary antibody was preincubated with an aromatase blocking peptide for 1 h, as well as KGN cells with (positive control) and without (negative control) forskolin treatment. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29898754/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aromatase Antibody-Simple Western-NB100-1596-img0009.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-31020241539251.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415392577.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415363733.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415293319.jpg)
![Immunohistochemistry: Aromatase Antibody [NB100-1596] - Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; n = 6) & hypothyroid (Hypo; solid bar; n = 6) rabbits. Representative immunoblot showing the expression of aromatase (a) & Ponceau's Red stained membrane (b). Relative expression of aromatase in C & Hypo groups (c). Data are mean ± SEM. ∗∗P < 0.01. Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) & hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) & (h) 100 μm; & (e) & (g) 20 μm. Granulose cells, gc; theca cells, tc. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28133606), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-310202415382447.jpg)
![Simple Western: Aromatase Antibody [NB100-1596] - Metformin decreases aromatase-positive, tumor-associated macrophages. a Quantification of aromatase-positive cells & b CD68-positive (CD68+) macrophages costaining for aromatase in the tumor border of tissues from control or metformin-treated rats. c Representative control & metformin-treated IHC images of CD68 & aromatase dual staining in tissue bordering mammary tumors (T) (arrows: yellow = CD68+aromatase+, brown = CD68+; scale bar = 50um). d Aromatase expression by Western blotting (WES system) from in vitro activated M1 & M2 rat macrophages using two different primary antibodies (Ab #1: Novus NB100-1596; Ab #2: clone 677, Baylor College of Medicine). Controls include ovary (positive control) & ovary in which the primary antibody was preincubated with an aromatase blocking peptide for 1 h, as well as KGN cells with (positive control) & without (negative control) forskolin treatment. e IHC intensity score for ER & PR (0 = no stain; 1 = weak; 2 = moderate; 3 = strong staining). *p < 0.05 Image collected & cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/s13058-018-0974-2), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1596_rabbit-polyclonal-aromatase-antibody-31020241682139.jpg)
![Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of GSK-3 beta in mouse beta cell line (betaTC3) using anti-GSK-3 beta antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Western-Blot-NBP1-47470-img0012.jpg "Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of GSK-3 beta in mouse beta cell line (betaTC3) using anti-GSK-3 beta antibody. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of NIH/3T3 (left) and U251 (right) cells using GSK3 beta mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Immunocytochemistry-Immunofluorescence-NBP1-47470-img0006.jpg)
![Immunohistochemistry: GSK-3 beta Antibody (3D10) [NBP1-47470] - Fap1-inhibition with SLV peptide increases phosphorylation of Fap1-substrates Fas and GSK-3 beta in a murine xenograft model. SW620 cells injected into flanks of athymic Nude mice & tumor volume was checked biweekly. Mice treated weekly with oxaliplatin (days 0, 7 and 14) & injected daily with Fap1 blocking SLV peptide, VLS control peptide, or treated with SLV or VLS peptide alone (n=12). Tumors were simultaneously harvested when control tumors were >2,000 mm3. SLV peptide increases Gsk3-phosphorylation with/without oxaliplatin. IF detection of total versus phospho- GSK-3 beta performed with DAPI staining of nuclei (areas without gland formation were selected). Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.25401), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Immunohistochemistry-NBP1-47470-img0014.jpg)
![Flow Cytometry: GSK-3 beta Antibody (3D10) [NBP1-47470] - An intracellular stain was performed on HeLa cells with GSK-3 beta (3D10) antibody NBP1-47470PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Flow-Cytometry-NBP1-47470-img0013.jpg)
![Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis using GSK3 beta mouse mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysates.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Western-Blot-NBP1-47470-img0008.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3 beta mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Immunohistochemistry-Paraffin-NBP1-47470-img0007.jpg)
![Flow Cytometry: GSK-3 beta Antibody (3D10) [NBP1-47470] - Flow cytometric analysis of Hela cells using GSK3 beta mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Flow-Cytometry-NBP1-47470-img0005.jpg)
![Simple Western: GSK-3 beta Antibody (3D10) [NBP1-47470] - Simple Western lane view shows a specific band for GSK-3 Beta in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Simple-Western-NBP1-47470-img0009.jpg)
![Simple Western: GSK-3 beta Antibody (3D10) [NBP1-47470] - Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice. Image courtesy of Dr. Brandi Wasek-Patterson at Baylor Research Institute, Institute of Metabolic Disease.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-3D10-Simple-Western-NBP1-47470-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody (3D10) - BSA Free [NBP1-47470] - Fap1-inhibition with SLV peptide increases Fas & Gsk3 beta phosphorylation in CD133+ cells in a murine xenograft modelSW620 cells were injected in the flanks of athymic Nude mice & tumor volume was determination biweekly. Mice were treated weekly with oxaliplatin (days 0, 7 & 14) & injected daily with Fap1 blocking SLV peptide or VLS control peptide, or treated with SLV or VLS peptide alone (n=12 per cohort). Tumors were simultaneously harvested from cohorts of mice when control tumors were >2,000 mm3. (A) SLV peptide increases Fas phosphorylation in CD133+ xenograft tumors with or without oxaliplatin. Immunofluorescent detection of phospho-Fas or CD133 was performed with DAPI staining of nuclei. (B) SLV peptide increases Gsk3 beta phosphorylation in CD133+ xenograft tumors with or without oxaliplatin. Immunofluorescent detection of phospho-Gsk3 beta or CD133 was performed with DAPI staining of nuclei. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29899829), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-47470_mouse-monoclonal-gsk-3-beta-antibody-3d10-310202416171462.jpg)
![Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody (3D10) - BSA Free [NBP1-47470] - Fap1-inhibition with SLV peptide increases phosphorylation of Fap1-substrates Fas & Gsk3 beta in a murine xenograft modelSW620 cells were injected in the flanks of athymic Nude mice & tumor volume was determination biweekly. Mice were treated weekly with oxaliplatin (days 0, 7 & 14) & injected daily with Fap1 blocking SLV peptide or VLS control peptide, or treated with SLV or VLS peptide alone (n=12 per cohort). Tumors were simultaneously harvested from cohorts of mice when control tumors were >2,000 mm3. (A) SLV peptide increases gland formation in xenograft tumors with or without oxaliplatin. Histology was analyzed by hematoxylin/ eosin staining. Fap1 expression was determined by immunofluorescence. Relative fluorescent intensity (RFI) of Fap1 staining is indicated below relevant panels. (B) SLV peptide increases Fas-phosphorylation in xenograft tumors with or without by oxaliplatin. Immunofluorescent detection of total versus phospho-Fas was performed with DAPI staining of nuclei. Areas without gland formation were selected for this study. (C) SLV peptide increases Gsk3 beta -phosphorylation with or without oxaliplatin. Immunofluorescent detection of total versus phospho- Gsk3 beta was performed with DAPI staining of nuclei. Areas without gland formation were selected for this study. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29899829), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-47470_mouse-monoclonal-gsk-3-beta-antibody-3d10-3102024161604.jpg)
![Immunohistochemistry: HSD3B1 Antibody (FDO66Q) [NB110-78644] - Staining of placental villi.](http://images.novusbio.com/fullsize/HSD3B1-Antibody-FDO66Q-Immunohistochemistry-NB110-78644-img0002.jpg "Immunohistochemistry: HSD3B1 Antibody (FDO66Q) [NB110-78644] - Staining of placental villi.")
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-23420241523259.jpg "Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.")
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-23420241523253.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-23420241523256.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-23420241523250.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, NB100-81946 was diluted at 1:200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-2342024155514.jpg)
![Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Western blot analysis of GSK-3 beta [p Tyr279, p Tyr216] expression in mouse testis (A), rat testis (B), rat spleen (C) whole cell lysates.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-234202415232519.jpg)
![Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Analysis of extracts of various cell lines, using NB100-81946](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-103202523472617.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81946(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-113202594524.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Methanol-fixed MEF cells.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-113202515145457.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Staining of Methanol-fixed Hela cells.](http://images.novusbio.com/fullsize/nb100-81946_rabbit-polyclonal-gsk-3-beta-p-tyr216-antibody-113202515145471.jpg)
![Western Blot: GSK-3 beta Antibody [NBP1-31353] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Western-Blot-NBP1-31353-img0015.jpg "Western Blot: GSK-3 beta Antibody [NBP1-31353] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody [NBP1-31353] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GSK3 beta stained by GSK3 beta antibody [C1C3] diluted at 1:500.Blue: Fluoroshield with DAPI. Scale bar= 10um.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31353-img0013.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody [NBP1-31353] - H661 xenograft, using GSK3 beta antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-31353-img0009.jpg)
![Western Blot: GSK-3 beta Antibody [NBP1-31353] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE, antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Western-Blot-NBP1-31353-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GSK-3 beta Antibody [NBP1-31353] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3].](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Western-Blot-NBP1-31353-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody [NBP1-31353] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSK3 beta protein stained by GSK3 beta antibody [C1C3] diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31353-img0006.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody [NBP1-31353] - Rat brain. GSK3 beta antibody [C1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-31353-img0007.jpg)
![Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining in human adrenal gland and cerebral cortex tissues using anti-CYP11A1 antibody. Corresponding CYP11A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Immunohistochemistry-Paraffin-NBP1-85368-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining in human adrenal gland and cerebral cortex tissues using anti-CYP11A1 antibody. Corresponding CYP11A1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CYP11A1 Antibody [NBP1-85368] - protein extract from: 1) human liver (negative), 2) human testis (positve), 3) MW marker (drawn with special ECL reagent marker), 4) human testis, 5) H295R adrenal cell line. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Western-Blot-NBP1-85368-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: CYP11A1 Antibody [NBP1-85368] - CYP11A1 expression (Leydig cells) present in early pubertal Rhesus monkey. Secondary antibody: Alexa Fluor488 anti rabbit. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Immunofluorescence-NBP1-85368-img0009.jpg)
![Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Immunohistochemistry-Paraffin-NBP1-85368-img0007.jpg)
![Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining of human adrenal gland shows high expression.](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Immunohistochemistry-Paraffin-NBP1-85368-img0006.jpg)
![Western Blot: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Wild-type (WT) and GSK3B knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Western-Blot-NBP3-13691-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Wild-type (WT) and GSK3B knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Rat brain. GSK-3 beta stained by GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Immunohistochemistry-Paraffin-NBP3-13691-img0005.jpg)
![Western Blot: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Western-Blot-NBP3-13691-img0001.jpg)
![Western Blot: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Western-Blot-NBP3-13691-img0007.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Mouse brain. GSK-3 beta stained by GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Immunohistochemistry-Paraffin-NBP3-13691-img0002.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Rat brain. GSK-3 beta stained by GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Immunohistochemistry-Paraffin-NBP3-13691-img0003.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Mouse brain. GSK-3 beta stained by GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT1619-Immunohistochemistry-Paraffin-NBP3-13691-img0004.jpg)
![Western Blot: CYP11A1 Antibody [NBP1-54758] - Sample Type: Hela Antibody Dilution: 1.0 ug/ml CYP11A1 is supported by BioGPS gene expression data to be expressed in HeLa](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Western-Blot-NBP1-54758-img0010.jpg "Western Blot: CYP11A1 Antibody [NBP1-54758] - Sample Type: Hela Antibody Dilution: 1.0 ug/ml CYP11A1 is supported by BioGPS gene expression data to be expressed in HeLa")
![Immunohistochemistry: CYP11A1 Antibody [NBP1-54758] - Human Placenta lysate tissue at an antibody concentration of 5.0 ug/ml using anti-CYP11A1 antibody](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Immunohistochemistry-NBP1-54758-img0009.jpg)
![Western Blot: CYP11A1 Antibody [NBP1-54758] - CYP11A1 antibody - middle region validated by WB using Hela cell lysate at 1ug/ml.](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Western-Blot-NBP1-54758-img0007.jpg)
![Western Blot: CYP11A1 Antibody [NBP1-54758] - Titration: 1 ug/ml Positive Control: Human HK2 cell (kidney proximal tubular cell line).](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Western-Blot-NBP1-54758-img0008.jpg)
![Western Blot: CYP11A1 Antibody [NBP1-54758] - Sample Type: Human Fetal Liver Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Western-Blot-NBP1-54758-img0011.jpg)
![Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-54758] - Human Placenta](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Immunohistochemistry-Paraffin-NBP1-54758-img0003.jpg)
![Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-54758] - Human Placenta](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Immunohistochemistry-Paraffin-NBP1-54758-img0004.jpg)
![Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-54758] - Human Placenta](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Immunohistochemistry-Paraffin-NBP1-54758-img0005.jpg)
![Western Blot: GSK-3 beta Antibody (GT16711) [NBP3-13679] - Wild-type (WT) and GSK-3 beta knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT16711] (NBP3-13679) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT16711-Western-Blot-NBP3-13679-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GSK-3 beta Antibody (GT16711) [NBP3-13679] - Wild-type (WT) and GSK-3 beta knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT16711] (NBP3-13679) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (GT16711) [NBP3-13679] - GSK-3 beta antibody detects GSK-3 beta protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSK-3 beta stained by GSK-3 beta antibody (NBP3-13679) diluted at 1:500.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT16711-Immunocytochemistry-Immunofluorescence-NBP3-13679-img0004.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT16711) [NBP3-13679] - GSK-3 beta antibody [GT16711] detects GSK-3 beta protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. GSK-3 beta stained by GSK-3 beta antibody [GT16711] (NBP3-13679) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT16711-Immunohistochemistry-Paraffin-NBP3-13679-img0003.jpg)
![Western Blot: GSK-3 beta Antibody (GT16711) [NBP3-13679] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT16711] (NBP3-13679) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT16711-Western-Blot-NBP3-13679-img0001.jpg)
![Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT16711) [NBP3-13679] - GSK-3 beta antibody [GT16711] detects GSK-3 beta protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. GSK-3 beta stained by GSK-3 beta antibody [GT16711] (NBP3-13679) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GSK-3-beta-Antibody-GT16711-Immunohistochemistry-Paraffin-NBP3-13679-img0002.jpg)
![Western Blot: CYP11A1 Antibody [NBP2-92879] - Analysis of extracts of HeLa cells, using CYP11A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Western-Blot-NBP2-92879-img0010.jpg "Western Blot: CYP11A1 Antibody [NBP2-92879] - Analysis of extracts of HeLa cells, using CYP11A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: CYP11A1 Antibody [NBP2-92879] - Analysis of extracts of various cell lines, using CYP11A1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Western-Blot-NBP2-92879-img0008.jpg)
![Western Blot: CYP11A1 Antibody [NBP2-92879] - Analysis of extracts of various cell lines, using CYP11A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Western-Blot-NBP2-92879-img0009.jpg)
![Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP2-92879] - Human colon using CYP11A1 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Immunohistochemistry-Paraffin-NBP2-92879-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: CYP11A1 Antibody - Azide and BSA Free [NBP2-92879] - Immunofluorescence analysis of paraffin-embedded human placenta using CYP11A1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92879_rabbit-polyclonal-cyp11a1-antibody-42202514251825.jpg)
![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA Antibody (5J4W7) [NBP3-15744] - Analysis of extracts of Mouse heart, using Acetyl-CoA Carboxylase alpha/ACACA Rabbit mAb (NBP3-15744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-5J4W7-Western-Blot-NBP3-15744-img0004.jpg "Western Blot: Acetyl-CoA Carboxylase alpha/ACACA Antibody (5J4W7) [NBP3-15744] - Analysis of extracts of Mouse heart, using Acetyl-CoA Carboxylase alpha/ACACA Rabbit mAb (NBP3-15744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: Acetyl-CoA Carboxylase alpha/ACACA Antibody (5J4W7) [NBP3-15744] - Mouse testis using Acetyl-CoA Carboxylase alpha/ACACA Rabbit mAb (NBP3-15744) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-5J4W7-Immunohistochemistry-Paraffin-NBP3-15744-img0002.jpg)
![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA Antibody (5J4W7) [NBP3-15744] - Analysis of extracts of various cell lines, using Acetyl-CoA Carboxylase alpha/ACACA Rabbit mAb (NBP3-15744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-5J4W7-Western-Blot-NBP3-15744-img0003.jpg)
![Immunohistochemistry-Paraffin: Acetyl-CoA Carboxylase alpha/ACACA Antibody (5J4W7) [NBP3-15744] - Rat kidney using Acetyl-CoA Carboxylase alpha/ACACA Rabbit mAb (NBP3-15744) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-5J4W7-Immunohistochemistry-Paraffin-NBP3-15744-img0001.jpg)
![Immunoprecipitation: Acetyl-CoA Carboxylase alpha/ACACA Antibody (5J4W7) [NBP3-15744] - Analysis of 300ug extracts of HepG2 cells using 3ug Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-15744). Western blot was performed from the immunoprecipitate using Acetyl-CoA Carboxylase alpha/ACACA (NBP3-15744) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-5J4W7-Immunoprecipitation-NBP3-15744-img0005.jpg)
![Western Blot: CYP11A1 Antibody - Azide and BSA Free [NBP2-92759] - Western blot analysis of lysates from Rat testis, using CYP11A1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 18s.](http://images.novusbio.com/fullsize/nbp2-92759_rabbit-polyclonal-cyp11a1-antibody-4220251429061.jpg "Western Blot: CYP11A1 Antibody - Azide and BSA Free [NBP2-92759] - Western blot analysis of lysates from Rat testis, using CYP11A1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 18s.")
![Western Blot: CYP11A1 Antibody [NBP2-92759] - Western blot analysis of extracts of Mouse testis, using CYP11A1 antibody (NBP2-92759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/CYP11A1-Antibody-Western-Blot-NBP2-92759-img0005.jpg)
![Western Blot: CYP11A1 Antibody - Azide and BSA Free [NBP2-92759] - Western blot analysis of various lysates using CYP11A1 Rabbit pAb at 1:800 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp2-92759_rabbit-polyclonal-cyp11a1-antibody-4220251430418.jpg)
![Immunocytochemistry/ Immunofluorescence: CYP11A1 Antibody - Azide and BSA Free [NBP2-92759] - Immunofluorescence analysis of paraffin-embedded human placenta using CYP11A1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92759_rabbit-polyclonal-cyp11a1-antibody-4220251430476.jpg)
![Immunohistochemistry: CYP11A1 Antibody - Azide and BSA Free [NBP2-92759] - Immunohistochemistry analysis of paraffin-embedded Rat ovary using CYP11A1 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-92759_rabbit-polyclonal-cyp11a1-antibody-42202514275222.jpg)