GSK-3 beta Antibody

Images

 
Western Blot: GSK-3 beta Antibody [NBP1-31353] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3].
Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody [NBP1-31353] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSK3 beta protein stained by GSK3 beta antibody [C1C3] diluted at ...read more
Immunohistochemistry-Paraffin: GSK-3 beta Antibody [NBP1-31353] - Paraffin-embedded H661 xenograft, using antibody at 1:500 dilution.
Western Blot: GSK-3 beta Antibody [NBP1-31353] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE, antibody diluted at 1:3000.
Western Blot: GSK-3 beta Antibody [NBP1-31353] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: GSK-3 beta Antibody [NBP1-31353] - Paraffin-embedded rat brain GSK3 beta antibody [C1C3] diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
     

Genetic Strategies

   

Order Details

GSK-3 beta Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human GSK3 beta. The exact sequence is proprietary.
Predicted Species
Porcine (90%), Bovine (98%), Sheep (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GSK3B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (83%). Xenopus laevis (82%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GSK-3 beta Antibody

  • EC 2.7.11
  • EC 2.7.11.26
  • glycogen synthase kinase 3 beta
  • glycogen synthase kinase-3 beta
  • GSK3 beta
  • GSK-3 beta
  • GSK3B
  • GSK3beta isoform

Background

Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. GSK3 has been implicated in fundamental cell processes such as cell fate determination, metabolism, transcriptional control, and oncogenesis. Two isoforms, alpha (GSK3A; OMIM 606784) and beta, show a high degree of amino acid homology within their catalytic domains. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
NBP2-25162
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP2-15071
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-46403
Species: Hu
Applications: IHC
NBP2-46404
Species: Hu
Applications: IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-33116
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-91228
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-32840
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC-P, IP
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
AF3287
Species: Hu, Mu, Rt
Applications: WB
NB100-56708
Species: Hu, Mu, Rt, Po, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
NBP2-15843
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-31353
Species: Hu, Mu, Rt, Po, Bv, Sh
Applications: WB, ICC/IF, IHC, IHC-P

Publications for GSK-3 beta Antibody (NBP1-31353) (0)

There are no publications for GSK-3 beta Antibody (NBP1-31353).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GSK-3 beta Antibody (NBP1-31353) (0)

There are no reviews for GSK-3 beta Antibody (NBP1-31353). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GSK-3 beta Antibody (NBP1-31353) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional GSK-3 beta Products

Bioinformatics Tool for GSK-3 beta Antibody (NBP1-31353)

Discover related pathways, diseases and genes to GSK-3 beta Antibody (NBP1-31353). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GSK-3 beta Antibody (NBP1-31353)

Discover more about diseases related to GSK-3 beta Antibody (NBP1-31353).
 

Pathways for GSK-3 beta Antibody (NBP1-31353)

View related products by pathway.

PTMs for GSK-3 beta Antibody (NBP1-31353)

Learn more about PTMs related to GSK-3 beta Antibody (NBP1-31353).
 

Research Areas for GSK-3 beta Antibody (NBP1-31353)

Find related products by research area.

Blogs on GSK-3 beta.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GSK-3 beta Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol GSK3B
Entrez