| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49533SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-54155SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22214-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2911SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47397-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-44298SS | |||
| Azide and BSA Free | NBP2-80753 | |||
![Western Blot: Glut4 Antibody [NBP1-49533] - Glycolysis-related genes and proteins were upregulated in differentiated podocytes. Representative blot images of glycolysis-related proteins (n =3). d All proteins were normalized to tubulin and compared to UDPs. *P < 0.05, **P < 0.01, determined by t test. Data are shown as the means +/- SD. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2481-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49533-img0015.jpg "Western Blot: Glut4 Antibody [NBP1-49533] - Glycolysis-related genes and proteins were upregulated in differentiated podocytes. Representative blot images of glycolysis-related proteins (n =3). d All proteins were normalized to tubulin and compared to UDPs. *P < 0.05, **P < 0.01, determined by t test. Data are shown as the means +/- SD. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2481-5), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Glut4 Antibody [NBP1-49533] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-GLUT4 [NBP1-49533] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Glut4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49533-img0011.jpg)
![Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in mouse kidney](http://images.novusbio.com/fullsize/Glut4-Antibody-Immunohistochemistry-NBP1-49533-img0005.jpg)
![Flow Cytometry: Glut4 Antibody - BSA Free [NBP1-49533] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP1-49533 (NBP1-49533AF647) on mouse Thymic Epithelial cells. Glut4 antibody in comparison to negative and IgG Control. Primary antibody dilution: 1:500. Image from verified customer review.](http://images.novusbio.com/fullsize/Glut4-Antibody---BSA-Free-Flow-Cytometry-NBP1-49533-img0016.jpg)
![Western Blot: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in A) MCF7 whole cell lysate and B) 3T3L1 whole cell lysate.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49533-img0006.jpg)
![Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from Human HeLa and A431, Mouse 3T3 and Rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Glut4 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49533-img0010.jpg)
![Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-Glut4 0.5 ug/ml in non-fat milk. Undiffer: undifferentiated; Differ: Differentiated. Image from verified customer review.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49533-img0014.jpg)
![Flow Cytometry: Glut4 Antibody [NBP1-49533] - GLUT4 Biotin/APC vs. [X] PE. Image from verified customer review.](http://images.novusbio.com/fullsize/Glut4-Antibody-Flow-Cytometry-NBP1-49533-img0008.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: Glut4 Antibody [NBP1-49533] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP1-49533. Staining of Glut 4 expression on Murine CD4+ T cells stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. This Alexa Fluor (R) 647 conjugated Glut 4 antibody (orange) positively stained mouse CD4+ T cells compared to Isotype Control (Rb IgG AF647, Novus NBP2-36463AF647, blue) and fluorescence minus one/FMO control (red). Image from verified customer review.](http://images.novusbio.com/fullsize/Glut4-Antibody-Flow-Cytometry-NBP1-49533-img0012.jpg)
![Flow (Intracellular): Glut4 Antibody [NBP1-49533] - An intracellular stain was performed on HepG2 with NBP1-49533 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/Glut4-Antibody-Flow-Intracellular-NBP1-49533-img0013.jpg)
![Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of Glut4 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp1-49533_rabbit-polyclonal-glut4-antibody-immunohistochemistry-paraffin-221020258369.jpg)
![Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of a FFPE tissue section of mouse small intestine using 1:200 dilution of Glut4 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp1-49533_rabbit-polyclonal-glut4-antibody-immunohistochemistry-paraffin-2210202583725.jpg)
![Western Blot: Lipase A Antibody [NBP1-54155] - Analysis using NBP1-54155 on cellular extracts of human prostate adenoma tissue, DU145, PC3, and 22RV1 prostate cancer cell lines, and HFL1 Normal primary human fibroblasts. NBP1-54155 revealed a strong specific band at the expected molecular weight for Lipase A at 45 kDa in different lysates of human cancer cell lines. NBP1-54155 specifically reveals LIPA in extracts from human prostate adenoma tissue and prostate cancer cell lines DU145, PC3, and 22RV1.The protein bands were visible in the positive but not in the negative controls. Therefore, NBP1-54155 was found to be suitable for checking the expression levels of Lipase A protein. Image from verified customer review.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Western-Blot-NBP1-54155-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: Lipase A Antibody [NBP1-54155] - Analysis using NBP1-54155 on cellular extracts of human prostate adenoma tissue, DU145, PC3, and 22RV1 prostate cancer cell lines, and HFL1 Normal primary human fibroblasts. NBP1-54155 revealed a strong specific band at the expected molecular weight for Lipase A at 45 kDa in different lysates of human cancer cell lines. NBP1-54155 specifically reveals LIPA in extracts from human prostate adenoma tissue and prostate cancer cell lines DU145, PC3, and 22RV1.The protein bands were visible in the positive but not in the negative controls. Therefore, NBP1-54155 was found to be suitable for checking the expression levels of Lipase A protein. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Western Blot: Lipase A Antibody [NBP1-54155] - NR and Metf promote FoxO1-mediated Lipa upregulation in visceral AT of adult mice. Adult C57/BL6 mice (5 months) were nutrient restricted (NR) by 24 h fasting or treated for 10 days with Metf (400 mg/kg) dissolved in drinking water (n=4 mice per group). Western blot of FoxO1 and Lipa in total protein extracts of explanted visceral (epididymal) AT. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2013404) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Western-Blot-NBP1-54155-img0009.jpg)
![Simple Western: Lipase A Antibody [NBP1-54155] - Image shows a specific band for Lipase A in 0.5 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Simple-Western-NBP1-54155-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Lipase A Antibody [NBP1-54155] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lipase A Antibody NBP1-54155 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54155-img0010.jpg)
![Immunohistochemistry-Paraffin: Lipase A Antibody - BSA Free [NBP1-54155] - Analysis of a FFPE tissue section of human stomach using 1:200 dilution of Lipase A antibody (NBP1-54155). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/Lipase-A-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-54155-img0011.jpg)
![Western Blot: Lipase A Antibody [NBP1-54155] - Detection of LIPA in HepG2 whole cell extract.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Western-Blot-NBP1-54155-img0001.jpg)
![Immunohistochemistry: Lipase A Antibody [NBP1-54155] - Staining of LIPA in mouse stomach smooth muscle.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunohistochemistry-NBP1-54155-img0005.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416154610.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416165629.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - AMPK drives Lipa-released FFAs oxidation restraining energetic catastrophe. (a) 3T3-L1 cells were transfected with DN-AMPK or empty vector. RT-qPCR analysis of relative peroxisome proliferator-activated receptor gamma-1 alpha , peroxisome proliferator-activated receptor-alpha , carnitine palmitoyltransferase 1b & acyl-CoA oxidase 1 mRNA levels were performed after 4 h of NR or 16 h of Metf treatment. Dashed line indicates the mRNA value of untreated DN-AMPK cells (Ctr). mRNA levels of untreated cells transfected with empty vector were similar to untreated DN-AMPK cells (data not shown). (b) Cheminoluminescent assay of ATP level in 3T3-L1 adipocytes transfected with DN-AMPK or empty vector after 8 h NR or 16 h Metf treatment. ATP level was expressed as pmol ATP per mg protein. (c) After 8 h of NR or 16 h Metf treatment, FFAs were enzymatically detected in culture medium of 3T3-L1 adipocytes transfected with DN-AMPK or empty vector. Values were expressed as μg FFAs per mg protein. (d) Left panel: western blot of AMPKpT172, PARP-1 & cleaved form of caspase-3 in 3T3-L1 adipocytes transfected with DN-AMPK or empty vector & subjected to 8 h NR. Right panel: cytofluorimetric analysis of apoptosis in DN-AMPK cells subjected to 8 h NR. (e) Western blot of PARP-1 & cleaved form of caspase-3 in 3T3-L1 adipocytes transfected with DN-AMPK or empty vector & treated with Metf for 16 h. (f) Western blot of FoxO1, Lipa, LC3 in 3T3-L1 adipocytes transfected with DN-AMPK or empty vector & subjected to 4 h NR. beta -actin was used as loading control. All values are given as mean±S.D. *P<0.05, **P<0.01 versus controls; °P<0.05, °°P<0.01 versus Metf treatment. All data are representative of at least three independent experiments Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-31020241618936.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416171493.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416171436.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - Lipa downregulation impairs lipid breakdown & elicits cell death in nutrient restricted adipocytes. (a) 3T3-L1 adipocytes were transfected with siRNA against Lipa (Lipa(−)) or with a scramble siRNA (Scr). Western blot of Lipa, PARP-1 & cleaved form of caspase-3 in total protein extracts from 3T3-L1 adipocytes after 4 h of NR. (b) TG content was quantified by ORO staining in fixed 3T3-L1 adipocytes 6 h after NR. (c) RT-qPCR analysis of relative peroxisome proliferator-activated receptor gamma-1 alpha , peroxisome proliferator-activated receptor-alpha & carnitine palmitoyltransferase 1b mRNA levels was performed in 3T3-L1 adipocytes 4 h after NR. (d) FFAs were analyzed in culture medium 6 h after NR. beta -actin was used as loading control. All values are given as mean±S.D. *P<0.05, **P<0.01 versus controls; °P<0.05 versus NR treatment. All data are representative of at least three independent experiments Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416163713.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-31020241616083.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - mouse kidney. FACL4 stained by FACL4 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NBP2-16401-img0012.jpg "Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - mouse kidney. FACL4 stained by FACL4 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: FACL4 Antibody [NBP2-16401] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FACL4-Antibody-Western-Blot-NBP2-16401-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: FACL4 Antibody [NBP2-16401] - analysis of FACL4 in MCF10A cells using anti-FACL4 antibody. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunofluorescence-NBP2-16401-img0005.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - Rat middle brain. FACL4 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NBP2-16401-img0008.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - Mouse lymph node. FACL4 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NBP2-16401-img0009.jpg)
![Immunoprecipitation: FACL4 Antibody [NBP2-16401] - Immunoprecipitation of FACL4 protein from HeLa whole cell extracts using 5 ug of FACL4 antibody [C3], C-term Western blot analysis was performed using FACL4 antibody [C3], C-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunoprecipitation-NBP2-16401-img0007.jpg)
![FACL4 antibody [C3], C-term detects FACL4 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.FACL4 stained by FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-immunohistochemistry-paraffin-261120251821100.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-western-blot-261120251832454.jpg)
![FACL4 antibody [C3], C-term detects FACL4 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FACL4 stained by FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-immunocytochemistry-immunofluorescence-2611202518263624.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-western-blot-26112025193395.jpg)
![FACL4 antibody [C3], C-term detects FACL4 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.FACL4 stained by FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-immunohistochemistry-paraffin-261120251841135.jpg)
![HG causes changes in the expression of ferroptosis-related proteins in 661W cells. (A) Western blot analysis of ferroptosis-related protein expression levels in HG-induced 661W cells. GAPDH was used as a control. (B) Expression of GPX4 and SLC7A11 proteins was significantly downregulated in HG-stimulated 661W cells after 12, 18, and 24 h. HG induced obvious upregulation in the expression of ACSL4, FTH1, and NCOA4 in 661W cells compared with the Ctrl group. (C) Immunofluorescence staining of localization of ferroptosis-related proteins (red) and nuclear (blue) in HG-induced 661W cells after 18 h. Data are shown as mean +/- SEM, n = 3 per group for Western blotting. p = not significant [ns], * p < 0.05, ** p < 0.01, *** p < 0.001 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-western-blot-13220261739389.jpg)
![Fer-1 treatment attenuated changes in ferroptosis-related proteins’ expression in HG-stimulated 661W cells after 18 h. (A) Western blot analysis of ferroptosis-related proteins’ expression levels in HG-induced 661W cells after Fer-1 treatment. GAPDH was used as a control. (B) The downregulation in GPX4 and SLC7A11 protein expression in HG-stimulated 661W cells was significantly attenuated after Fer-1 treatment. The upregulation in ACSL4, FTH1, and NCOA4 protein expression in HG-stimulated 661W cells was effectively abrogated after Fer-1 treatment. (C) Immunofluorescence staining of ferroptosis-related proteins (red) and nuclear (blue) in HG-induced 661W cells after Fer-1 administration. Data are shown as mean +/- SEM, n = 3 per group for Western blotting. p = not significant [ns], * p < 0.05, ** p < 0.01 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-western-blot-132202617595722.jpg)
![Fer-1 administration attenuated changes of ferroptosis-related proteins expression in diabetic mice retinas. (A) Western blot analysis of ferroptosis-related proteins expression levels in diabetic mice retinas after Fer-1 treatment. GAPDH was used as a control. (B) The decrease in GPX4 and SLC7A11 protein expression in diabetic mice retinas was significantly attenuated after Fer-1 treatment. The increase in ACSL4, FTH1, and NCOA4 protein expression in diabetic mice retinas was effectively abrogated after Fer-1 treatment. (C) Immunofluorescence staining of ferroptosis-related proteins (red) and nuclear (blue) in diabetic mice retinas after Fer-1 treatment: ganglion cell layer (GCL), inner nuclear layer (INL), outer nuclear layer (ONL), inner segment (IS), outer segment (OS). Data are shown as mean +/- SEM, n = 3 per group for Western blotting. p = not significant [ns], * p < 0.05, ** p < 0.01 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-western-blot-13220261755394.jpg)
![HG causes changes in the expression of ferroptosis-related proteins in 661W cells. (A) Western blot analysis of ferroptosis-related protein expression levels in HG-induced 661W cells. GAPDH was used as a control. (B) Expression of GPX4 and SLC7A11 proteins was significantly downregulated in HG-stimulated 661W cells after 12, 18, and 24 h. HG induced obvious upregulation in the expression of ACSL4, FTH1, and NCOA4 in 661W cells compared with the Ctrl group. (C) Immunofluorescence staining of localization of ferroptosis-related proteins (red) and nuclear (blue) in HG-induced 661W cells after 18 h. Data are shown as mean +/- SEM, n = 3 per group for Western blotting. p = not significant [ns], * p < 0.05, ** p < 0.01, *** p < 0.001 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-immunocytochemistry-immunofluorescence-132202619213323.jpg)
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Western blot analysis of Muscle Phosphofructokinase/PFKM/PFK-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: PC-3M cell lysate](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Western-Blot-NBP2-75578-img0011.jpg "Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Western blot analysis of Muscle Phosphofructokinase/PFKM/PFK-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: PC-3M cell lysate")
![Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Staining Fructose 6 Phosphate Kinase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunocytochemistry-Immunofluorescence-NBP2-75578-img0004.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Analysis of paraffin-embedded rat brain tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunohistochemistry-Paraffin-NBP2-75578-img0009.jpg)
![Flow Cytometry: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Analysis of SH-SY-5Y cells with Fructose 6 Phosphate Kinase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Flow-Cytometry-NBP2-75578-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Staining Fructose 6 Phosphate Kinase in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunocytochemistry-Immunofluorescence-NBP2-75578-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Staining Fructose 6 Phosphate Kinase in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunocytochemistry-Immunofluorescence-NBP2-75578-img0003.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Analysis of paraffin-embedded human liver cancer tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunohistochemistry-Paraffin-NBP2-75578-img0005.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Analysis of paraffin-embedded human thyroid gland tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunohistochemistry-Paraffin-NBP2-75578-img0006.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Analysis of paraffin-embedded human kidney tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunohistochemistry-Paraffin-NBP2-75578-img0007.jpg)
![Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-JU53-31-Immunohistochemistry-Paraffin-NBP2-75578-img0008.jpg)
![Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - Western blot analysis using Glucose Transporter GLUT4 mouse mAb against HeLa (1), NIH3T3 (2), 3T3-L1 (3) cell lysate and Mouse heart (4) tissue lysate.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Western-Blot-NBP2-22214-img0008.jpg "Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - Western blot analysis using Glucose Transporter GLUT4 mouse mAb against HeLa (1), NIH3T3 (2), 3T3-L1 (3) cell lysate and Mouse heart (4) tissue lysate.")
![Immunocytochemistry/Immunofluorescence: Glut4 Antibody (3G10A3) [NBP2-22214] - Analysis of HepG2 cells using Glucose Transporter GLUT4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Immunocytochemistry-Immunofluorescence-NBP2-22214-img0003.jpg)
![Immunohistochemistry-Paraffin: Glut4 Antibody (3G10A3) [NBP2-22214] - analysis of cardiac muscle tissues using SLC2A4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Immunohistochemistry-Paraffin-NBP2-22214-img0009.jpg)
![Flow Cytometry: Glut4 Antibody (3G10A3) [NBP2-22214] - Flow cytometric analysis of HeLa cells using Glucose Transporter GLUT4 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Flow-Cytometry-NBP2-22214-img0001.jpg)
![Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - Western blot analysis using Glucose Transporter Glut4 Antibody (3G10A3)mAb against HEK293 (1) and Glucose Transporter GLUT4 (AA: 224-353)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Western-Blot-NBP2-22214-img0006.jpg)
![Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - human Glucose Transporter GLUT4 recombinant protein.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Western-Blot-NBP2-22214-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Glut4 Antibody (3G10A3) [NBP2-22214] - Analysis of HeLa cells using Glucose Transporter GLUT4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Immunocytochemistry-Immunofluorescence-NBP2-22214-img0002.jpg)
![Immunohistochemistry-Paraffin: Glut4 Antibody (3G10A3) [NBP2-22214] - Analysis of paraffin-embedded bladder cancer tissues using Glucose Transporter GLUT4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Immunohistochemistry-Paraffin-NBP2-22214-img0004.jpg)
![ELISA: Glut4 Antibody (3G10A3) [NBP2-22214] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-ELISA-NBP2-22214-img0005.jpg)
![mTORC2 in adipose tissue is required for cold‐induced glucose uptake and glycolysis2‐deoxyglucose‐6‐phosphate (2DG6P) accumulation in BAT of AdRiKO and control mice housed at 22 or at 4C for 4 h (n = 6/group).Extracellular acidification rate (ECAR) of BAT explants from AdRiKO and control mice housed at 22 or at 4C for 4 h (n = 7/group).Immunoblot analysis of BAT from AdRiKO and control mice housed at 22 or at 4C for 8 h for the indicated proteins (n = 6/group, each lane represents a mix of 3 mice).Immunoblot analysis of isolated plasma membranes from BAT of AdRiKO and control mice housed at 22 or at 4C for 8 h for the indicated proteins (n = 6/group, each lane represents a mix of 3 mice).Immunoblot analysis of mitochondrial and cytosolic fractions from BAT of AdRiKO and control mice housed at 22 or at 4C for 4 h for the indicated proteins (n = 6/group, each lane represents a mix of 3 mice).Cytosolic hexokinase activity in BAT of AdRiKO and control mice housed at 22 or at 4C for 4 h [n = 5 (control 22C), n = 5 (AdRiKO 22C), n = 7 (control 4C), n = 7 (AdRiKO 4C)].Mitochondrial hexokinase activity in BAT of AdRiKO and control mice housed at 22 or at 4C for 4 h [n = 5 (control 22C), n = 5 (AdRiKO 22C), n = 7 (control 4C), n = 7 (AdRiKO 4C)].Data information: Data represent mean +/- SEM. Statistically significant differences between AdRiKO and control mice were determined with unpaired Student's t‐test and are indicated with asterisks (*P < 0.05). Statistically significant differences between temperatures were determined with unpaired Student's t‐test and are indicated with a number sign (#P < 0.05; ##P < 0.01; ###P < 0.001). The exact P‐value for each significant difference can be found in Appendix Table S2. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26772600), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-22214_mouse-monoclonal-glut4-antibody-3g10a3-western-blot-132202615255419.jpg)
![Western Blot: Ceramide Kinase Antibody [NB100-2911] - Detection of Ceramide Kinase in A549 cell lysate using NB100-2911.](http://images.novusbio.com/fullsize/Ceramide-Kinase-Antibody-Western-Blot-NB100-2911-img0003.jpg "Western Blot: Ceramide Kinase Antibody [NB100-2911] - Detection of Ceramide Kinase in A549 cell lysate using NB100-2911.")
![Immunocytochemistry/Immunofluorescence: Ceramide Kinase Antibody [NB100-2911] - Ceramide Kinase antibody was tested in A431 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Ceramide-Kinase-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2911-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: FACL4 Antibody [NBP2-16400] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FACL4 protein stained by FACL4 antibody [N1N3] diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16400-img0002.jpg "Immunocytochemistry/Immunofluorescence: FACL4 Antibody [NBP2-16400] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FACL4 protein stained by FACL4 antibody [N1N3] diluted at 1:200. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16400] - Paraffin-embedded mouse kidney. FACL4 antibody [N1N3] diluted at 1:500.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NBP2-16400-img0011.jpg)
![Western Blot: FACL4 Antibody [NBP2-16400] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/FACL4-Antibody-Western-Blot-NBP2-16400-img0001.jpg)
![Western Blot: FACL4 Antibody [NBP2-16400] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FACL4-Antibody-Western-Blot-NBP2-16400-img0007.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16400] - Paraffin-embedded rat duodenum. FACL4 antibody [N1N3] diluted at 1:500](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NBP2-16400-img0010.jpg)
![Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (NBP2-16400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16400_rabbit-polyclonal-facl4-antibody-western-blot-261120251827016.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (NBP2-16400) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-16400_rabbit-polyclonal-facl4-antibody-western-blot-261120251823622.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (NBP2-16400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16400_rabbit-polyclonal-facl4-antibody-western-blot-2611202518204710.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (NBP2-16400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16400_rabbit-polyclonal-facl4-antibody-western-blot-2611202518422918.jpg)
![Immunoprecipitation: FACL4 Antibody (3I8D10) [NBP3-16012] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug FACL4 antibody (NBP3-16012). Western blot was performed from the immunoprecipitate using FACL4 antibody (NBP3-16012) at a dilition of 1:2000.](http://images.novusbio.com/fullsize/FACL4-Antibody-3I8D10-Immunoprecipitation-NBP3-16012-img0002.jpg "Immunoprecipitation: FACL4 Antibody (3I8D10) [NBP3-16012] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug FACL4 antibody (NBP3-16012). Western blot was performed from the immunoprecipitate using FACL4 antibody (NBP3-16012) at a dilition of 1:2000.")
![Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-5220251718945.jpg)
![Western Blot: FACL4 Antibody (4W3T9) [FACL4] - Western blot analysis of various lysates using FACL4 Rabbit mAb at 1:58000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517184534.jpg)
![Western Blot: FACL4 Antibody (4W3T9) [FACL4] - Western blot analysis of lysates from Rat liver using FACL4 Rabbit mAb at 1:58000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517202918.jpg)
![Western Blot: FACL4 Antibody (4W3T9) [FACL4] - Western blot analysis of lysates from Hep G2 cells using FACL4 Rabbit mAb at 1:58000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517184563.jpg)
![Immunocytochemistry/ Immunofluorescence: FACL4 Antibody (4W3T9) [FACL4] - Confocal imaging of Hep G2 cells using FACL4 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517172885.jpg)
![Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Rat brain using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517213510.jpg)
![Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517213524.jpg)
![Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Human kidney using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517172887.jpg)
![Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL44] (NBP3-13666) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FACL4-Antibody-HL229-Western-Blot-NBP3-13666-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL44] (NBP3-13666) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: FACL4 Antibody (HL229) [NBP3-13666] - FACL4 antibody [HL229] detects FACL4 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FACL4 stained by FACL4 antibody [HL229] (NBP3-13666) diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/FACL4-Antibody-HL229-Immunocytochemistry-Immunofluorescence-NBP3-13666-img0002.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody (HL229) [NBP3-13666] - FACL4 antibody [HL229] detects FACL4 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. FACL4 stained by FACL4 antibody [HL229] (NBP3-13666) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/FACL4-Antibody-HL229-Immunohistochemistry-Paraffin-NBP3-13666-img0003.jpg)
![Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] (NBP3-13666) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FACL4-Antibody-HL229-Western-Blot-NBP3-13666-img0004.jpg)
![Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] (NBP3-13666) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FACL4-Antibody-HL229-Western-Blot-NBP3-13666-img0005.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody (HL229) [NBP3-13666] - FACL4 antibody [HL44] detects FACL4 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.FACL4 stained by FACL4 antibody [HL44] (NBP3-13666) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13666_rabbit-facl4-mab-hl229-910202419593532.jpg)
![Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Drosophila tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL44] (NBP3-13666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/nbp3-13666_rabbit-facl4-mab-hl229-910202420122075.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: FACL4 Antibody (HL229) [NBP3-13666] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [HL229] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13666_rabbit-facl4-mab-hl229-1610202419414568.jpg)
![Immunohistochemistry-Paraffin: Lipase A Antibody [NBP2-47397] - Analysis in human spleen and skeletal muscle tissues. Corresponding Lipase A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunohistochemistry-Paraffin-NBP2-47397-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Lipase A Antibody [NBP2-47397] - Analysis in human spleen and skeletal muscle tissues. Corresponding Lipase A RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Lipase A Antibody [NBP2-47397] - Analysis in human spleen tissue.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Western-Blot-NBP2-47397-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Lipase A Antibody [NBP2-47397] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47397-img0003.jpg)
![Immunohistochemistry-Paraffin: Lipase A Antibody [NBP2-47397] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunohistochemistry-Paraffin-NBP2-47397-img0015.jpg)
![Immunohistochemistry-Paraffin: Lipase A Antibody [NBP2-47397] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunohistochemistry-Paraffin-NBP2-47397-img0008.jpg)
![Immunohistochemistry-Paraffin: Lipase A Antibody [NBP2-47397] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunohistochemistry-Paraffin-NBP2-47397-img0009.jpg)
![Immunohistochemistry-Paraffin: Lipase A Antibody [NBP2-47397] - Staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunohistochemistry-NBP2-47397-img0011.jpg)
![Immunohistochemistry-Paraffin: Lipase A Antibody [NBP2-47397] - Staining of human lymphoid tissues shows strong cytoplasmic positivity in a subset of non-germinal center cells.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunohistochemistry-NBP2-47397-img0012.jpg)
![Western Blot: FACL4 Antibody [NB300-861] - Staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/FACL4-Antibody-Western-Blot-NB300-861-img0001.jpg "Western Blot: FACL4 Antibody [NB300-861] - Staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: FACL4 Antibody [NB300-861] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi and Endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunocytochemistry-Immunofluorescence-NB300-861-img0002.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody [NB300-861] - Staining of paraffin embedded Human Cortex. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NB300-861-img0005.jpg)
![Flow Cytometry: FACL4 Antibody [NB300-861] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/FACL4-Antibody-Flow-Cytometry-NB300-861-img0003.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody [NB300-861] - Staining of paraffin embedded Human Small Intestine. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NB300-861-img0004.jpg)
![Western Blot: Glut4 Antibody (6H11NB) [NBP2-44298] - Analysis of GLUT4 [6H11BN] in partial recombinant GLUT4 protein.](http://images.novusbio.com/fullsize/Glut4-Antibody-6H11NB-Western-Blot-NBP2-44298-img0002.jpg "Western Blot: Glut4 Antibody (6H11NB) [NBP2-44298] - Analysis of GLUT4 [6H11BN] in partial recombinant GLUT4 protein.")
![Immunohistochemistry-Paraffin: Glut4 Antibody (6H11NB) [NBP2-44298] - Analysis of a FFPE tissue section of normal human skeletal muscle using 1:300 dilution of Glut4 antibody (clone 6H11NB). The antibody generated a diffused staining in the blood vessels as well as the cytoplasm/sarcoplasma of skeletal muscles while the nuclei did not show any positivity for Glut4.](http://images.novusbio.com/fullsize/Glut4-Antibody-6H11NB-Immunohistochemistry-Paraffin-NBP2-44298-img0003.jpg)
![Western Blot: Glut4 Antibody (6H11NB) [NBP2-44298] - Analysis of human heart tissue lysate using 1:500 dilution of GLUT4 antibody [clone 6H11NB]. The antibody generated a single specific band of Glut4 at ~55 kDa molecular weight position.](http://images.novusbio.com/fullsize/Glut4-Antibody-6H11NB-Western-Blot-NBP2-44298-img0001.jpg)