| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-39113SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49533SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22218SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05054SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89324-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22214-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22212-0.025ml | |||
![Western Blot: Glut1 Antibody [NB110-39113] - PrP-mediated increase in IC iron downregulates glucose transporters in the brain, neuroretina, and the liver: A similar evaluation of retinal lysates shows upregulation of Glut1 in PrP-/- relative to Tg40 PrP samples (lanes 1 & 3). Iron overloading downregulates Glut1 in Tg40 PrP samples relative to untreated controls, but has minimal effect on similarly treated PrP-/- samples (lanes 2 & 4). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-24786-1) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glut1-Antibody-Western-Blot-NB110-39113-img0019.jpg "<b>Biological Strategies Validation. </b>Western Blot: Glut1 Antibody [NB110-39113] - PrP-mediated increase in IC iron downregulates glucose transporters in the brain, neuroretina, and the liver: A similar evaluation of retinal lysates shows upregulation of Glut1 in PrP-/- relative to Tg40 PrP samples (lanes 1 & 3). Iron overloading downregulates Glut1 in Tg40 PrP samples relative to untreated controls, but has minimal effect on similarly treated PrP-/- samples (lanes 2 & 4). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-24786-1) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: Glut1 Antibody [NB110-39113] - Western blot of GLUT1 on mouse kidney membrane protein (lane A) and rat kidney membrane protein (lane B).](http://images.novusbio.com/fullsize/Glut1-Antibody-Western-Blot-NB110-39113-img0006.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NB110-39113] - Immunohistochemical analysis of FFPE tissue section of human placenta using 1:200 dilution of Glut1 antibody. The staining was developed using HRP-DAB detection method and the sections were further counterstained with hematoxylin. This antibody generated a specific strong membrane cytoplasmic staining of Glut1 primarily in the syncytiotrophoblast layers of various villi and in the red blood cells (RBCs). Cytotrophoblasts showed a very weak expression of this protein.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NB110-39113-img0011.jpg)
![Flow Cytometry: Glut1 Antibody [NB110-39113] - An intracellular stain was performed on HepG2 with NB110-39113 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/Glut1-Antibody-Flow-Cytometry-NB110-39113-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Glut1 Antibody [NB110-39113] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-GLUT1 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-39113-img0013.jpg)
![Western Blot: Glut1 Antibody [NB110-39113] - Analysis of HeLa lysates using NB110-39113. Image courtesy of Gregg Semenza (PMID: 21620138).](http://images.novusbio.com/fullsize/Glut1-Antibody-Western-Blot-NB110-39113-img0007.jpg)
![Western Blot: Glut1 Antibody [NB110-39113] - Glut1 in human brain lysate (55 kDa). Antibody at 1:500. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Glut1-Antibody-Western-Blot-NB110-39113-img0016.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: Glut1 Antibody [NB110-39113] - Flow cytometry analysis using the PE conjugate of NB110-39113. Staining of Glut 1 expression on CD4+ T cells stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. FMO control (red) and isotype control (blue, NBP2-24983) were compared to CD4+ T cells (orange), and this PE conjugated Glut 1 antibody positively stained CD4+ lymphocytes isolated from Mouse. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Glut1-Antibody-Flow-Cytometry-NB110-39113-img0014.jpg)
![Immunocytochemistry/ Immunofluorescence: Glut1 Antibody - BSA Free [NB110-39113] - LAIR-1 inhibits Glut1-related glucose uptake in OS cells. a Heatmap showing the levels of differentially expressed mRNAs. b Top 20 KEGG pathway annotation categories for target gene functions of predicted mRNAs. c Selected significantly differentially expressed mRNA-related to EMT in RNA-seq data between two groups, ***P < 0.001. d qPCR validation of differentially expressed EMT-related genes in LV-NC & LV-LAIR-1-overexpressing OS cells, **P < 0.01. e Glut1 expression analyzed by western blotting. f Immunofluorescence staining of Glut1 in the LV-LAIR-1-overexpressing OS cells. Scale bar = 50 μm. Data were obtained from at least two independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32563267), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-39113_rabbit-polyclonal-glut1-antibody-310202415175286.jpg)
![Western Blot: Glut1 Antibody - BSA Free [NB110-39113] - LAIR-1 inhibits Glut1-related glucose uptake in OS cells. a Heatmap showing the levels of differentially expressed mRNAs. b Top 20 KEGG pathway annotation categories for target gene functions of predicted mRNAs. c Selected significantly differentially expressed mRNA-related to EMT in RNA-seq data between two groups, ***P < 0.001. d qPCR validation of differentially expressed EMT-related genes in LV-NC & LV-LAIR-1-overexpressing OS cells, **P < 0.01. e Glut1 expression analyzed by western blotting. f Immunofluorescence staining of Glut1 in the LV-LAIR-1-overexpressing OS cells. Scale bar = 50 μm. Data were obtained from at least two independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32563267), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-39113_rabbit-polyclonal-glut1-antibody-310202415334922.jpg)
![Simple Western: Glut1 Antibody - BSA Free [NB110-39113] - Protein changes in ONHAs corroborate bioenergetics data. (A) Glucose transporter-1 protein levels in Stretched ONH astrocytes are significantly higher than Control (*p = 0.0225, n = 7 Control, n = 8 Stretch). Retinal lysate from a 2 month-old mouse was used as a positive control for each protein analyzed, while negative control was the signal obtained when no primary antibody was included in the capillary. (B) Lactate dehydrogenase-A, the astrocyte-specific isoform of the enzyme that catalyzes the interconversion of pyruvate & lactate, has equivalent protein levels in Control & Stretch ONH astrocytes. (C) Glucose-6-phosphate dehydrogenase, the enzyme that shunts glucose into the pentose phosphate pathway, is no different in Control & Stretch ONH astrocytes. (D) Glutamine synthetase, the enzyme that synthesizes glutamine from glutamate, is no different in Control & Stretch ONH astrocytes. (E) The monomeric form of glutamate-aspartate transporter (GLAST) has significantly higher protein levels in the Stretch as compared to the Control ONH astrocytes (p = 0.020; n = 4 Control, n = 5 Stretch). (F) GLAST dimer protein levels are no different in Control & Stretch ONH astrocytes. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35992925), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-39113_rabbit-polyclonal-glut1-antibody-310202416284917.jpg)
![Western Blot: Glut4 Antibody [NBP1-49533] - Glycolysis-related genes and proteins were upregulated in differentiated podocytes. Representative blot images of glycolysis-related proteins (n =3). d All proteins were normalized to tubulin and compared to UDPs. *P < 0.05, **P < 0.01, determined by t test. Data are shown as the means +/- SD. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2481-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49533-img0015.jpg "Western Blot: Glut4 Antibody [NBP1-49533] - Glycolysis-related genes and proteins were upregulated in differentiated podocytes. Representative blot images of glycolysis-related proteins (n =3). d All proteins were normalized to tubulin and compared to UDPs. *P < 0.05, **P < 0.01, determined by t test. Data are shown as the means +/- SD. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2481-5), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Glut4 Antibody [NBP1-49533] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-GLUT4 [NBP1-49533] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Glut4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49533-img0011.jpg)
![Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in mouse kidney](http://images.novusbio.com/fullsize/Glut4-Antibody-Immunohistochemistry-NBP1-49533-img0005.jpg)
![Flow Cytometry: Glut4 Antibody - BSA Free [NBP1-49533] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP1-49533 (NBP1-49533AF647) on mouse Thymic Epithelial cells. Glut4 antibody in comparison to negative and IgG Control. Primary antibody dilution: 1:500. Image from verified customer review.](http://images.novusbio.com/fullsize/Glut4-Antibody---BSA-Free-Flow-Cytometry-NBP1-49533-img0016.jpg)
![Western Blot: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in A) MCF7 whole cell lysate and B) 3T3L1 whole cell lysate.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49533-img0006.jpg)
![Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from Human HeLa and A431, Mouse 3T3 and Rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Glut4 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49533-img0010.jpg)
![Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-Glut4 0.5 ug/ml in non-fat milk. Undiffer: undifferentiated; Differ: Differentiated. Image from verified customer review.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49533-img0014.jpg)
![Flow Cytometry: Glut4 Antibody [NBP1-49533] - GLUT4 Biotin/APC vs. [X] PE. Image from verified customer review.](http://images.novusbio.com/fullsize/Glut4-Antibody-Flow-Cytometry-NBP1-49533-img0008.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: Glut4 Antibody [NBP1-49533] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP1-49533. Staining of Glut 4 expression on Murine CD4+ T cells stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. This Alexa Fluor (R) 647 conjugated Glut 4 antibody (orange) positively stained mouse CD4+ T cells compared to Isotype Control (Rb IgG AF647, Novus NBP2-36463AF647, blue) and fluorescence minus one/FMO control (red). Image from verified customer review.](http://images.novusbio.com/fullsize/Glut4-Antibody-Flow-Cytometry-NBP1-49533-img0012.jpg)
![Flow (Intracellular): Glut4 Antibody [NBP1-49533] - An intracellular stain was performed on HepG2 with NBP1-49533 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/Glut4-Antibody-Flow-Intracellular-NBP1-49533-img0013.jpg)
![Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of Glut4 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp1-49533_rabbit-polyclonal-glut4-antibody-immunohistochemistry-paraffin-221020258369.jpg)
![Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of a FFPE tissue section of mouse small intestine using 1:200 dilution of Glut4 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp1-49533_rabbit-polyclonal-glut4-antibody-immunohistochemistry-paraffin-2210202583725.jpg)
![Western Blot: Glut1 Antibody [NB300-666] - Western blot analysis of 786-mock cells stably expressing shRNA constructs. For HIF2A (NB100-480) and HIF1B (NB100-124) analysis, nuclear extracts were generated and analyzed. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0023936) licensed under a CC-BY license. Glut-1 was detected using NB300-666 in whole cell extracts.](http://images.novusbio.com/fullsize/Glut1-Antibody-Western-Blot-NB300-666-img0007.jpg "Western Blot: Glut1 Antibody [NB300-666] - Western blot analysis of 786-mock cells stably expressing shRNA constructs. For HIF2A (NB100-480) and HIF1B (NB100-124) analysis, nuclear extracts were generated and analyzed. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0023936) licensed under a CC-BY license. Glut-1 was detected using NB300-666 in whole cell extracts.")
![Western Blot: Glut1 Antibody [NB300-666] - WB analysis of purified Glucose Transporter (GLUT1) with GLUT1 antibody at a dilution of 1:2000.](http://images.novusbio.com/fullsize/Glut1-Antibody-Western-Blot-NB300-666-img0001.jpg)
![Western Blot: Glut1 Antibody [NB300-666] - Upregulation of Glut1 (NB300-666) and Glut3 (NBP2-66872) expression by Aqp9 gene deletion and ONC. Western blots for Glut1 and Glut3 protein expression levels in WT and Aqp9 KO mice with and without ONC. beta actin was used as a control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32748371/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glut1-Antibody-Western-Blot-NB300-666-img0008.jpg)
![Western Blot: Glut1 Antibody [NB300-666] - Rat brain cortical extracts (10 ug total protein per lane) on 10% SDS-PAGE gel. Antibody diluted 1:2000.](http://images.novusbio.com/fullsize/Glut1-Antibody-Western-Blot-NB300-666-img0006.jpg)
![Western Blot: Glut2 Antibody [NBP2-22218] - Analysis of Glut2 in total lysates of MIN6 cells using anti-Glut2 (NBP2-22218) antibody at 1/500 dilution. Image was submitted via customer review.](http://images.novusbio.com/fullsize/Glut2-Antibody-Western-Blot-NBP2-22218-img0008.jpg "Western Blot: Glut2 Antibody [NBP2-22218] - Analysis of Glut2 in total lysates of MIN6 cells using anti-Glut2 (NBP2-22218) antibody at 1/500 dilution. Image was submitted via customer review.")
![Immunocytochemistry/Immunofluorescence: Glut2 Antibody [NBP2-22218] - Glut2 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Glut2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22218-img0005.jpg)
![Immunohistochemistry-Paraffin: Glut2 Antibody [NBP2-22218] - Histology and immunostaining of pancreas in treated and untreated mice with STZ. (i, iv) H-E of a pancreatic islet in untreated and STZ-WT mice; (ii, v) and (iii,vi) reduction of Glut2 and insulin expression in STZ-WT mice compared to control mice. B. ( Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0105550), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glut2-Antibody-Immunohistochemistry-Paraffin-NBP2-22218-img0009.jpg)
![Western Blot: Glut2 Antibody [NBP2-22218] - Analysis of Glucose Transporter GLUT2 in human pancreas.](http://images.novusbio.com/fullsize/Glut2-Antibody-Western-Blot-NBP2-22218-img0007.jpg)
![Immunohistochemistry: Glut2 Antibody [NBP2-22218] - Staining of Glucose Transporter GLUT2 in mouse liver.](http://images.novusbio.com/fullsize/Glut2-Antibody-Immunohistochemistry-NBP2-22218-img0006.jpg)
![Immunohistochemistry-Paraffin: Glut2 Antibody [NBP2-22218] - Histology and immunostaining of pancreas in treated and untreated mice with STZ. (i, iv) H-E of a pancreatic islet in untreated and STZ-K-RasV12 mice; (ii, v) and (iii,vi) reduction of Glut2 and insulin expression in STZ-K-RasV12 mice compared to untreated K-RasV12 control mice. Magnification 200x. Calibration bar: 50 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0105550), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glut2-Antibody-Immunohistochemistry-Paraffin-NBP2-22218-img0010.jpg)
![Immunohistochemistry: GLUT9 Antibody [NBP1-05054] - Immunofluorescence staining of GLUT9 in PFA-fixed murine brain sections. Frozen sections of paraformaldehyde-fixed wild-type murine brain were used for immunofluorescence staining. a, b Antigen absorption test. Immunofluorescence staining of the ependymal wall of the dorsal third ventricle using a anti-GLUT9 antibody and b antigen-preabsorbed antibody. Scale bar 100 um. c, d Immunofluorescence staining of GLUT9 (magenta), acetylated-tubulin (Ac-Tubulin, green) and DAPI (blue) on ependymal cells. Scale bar 10 um. e-g Immunofluorescence staining of GLUT9 (magenta) and NeuN (green) showing co-localization in neurons. Scale bar 10 um. Image collected and cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-NBP1-05054-img0006.jpg "Immunohistochemistry: GLUT9 Antibody [NBP1-05054] - Immunofluorescence staining of GLUT9 in PFA-fixed murine brain sections. Frozen sections of paraformaldehyde-fixed wild-type murine brain were used for immunofluorescence staining. a, b Antigen absorption test. Immunofluorescence staining of the ependymal wall of the dorsal third ventricle using a anti-GLUT9 antibody and b antigen-preabsorbed antibody. Scale bar 100 um. c, d Immunofluorescence staining of GLUT9 (magenta), acetylated-tubulin (Ac-Tubulin, green) and DAPI (blue) on ependymal cells. Scale bar 10 um. e-g Immunofluorescence staining of GLUT9 (magenta) and NeuN (green) showing co-localization in neurons. Scale bar 10 um. Image collected and cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x) licensed under a CC-BY license.")
![Immunohistochemistry: GLUT9 Antibody [NBP1-05054] - GLUT9 in methacarn-fixed murine brain. Paraffin sections of methacarn-fixed murine brain were used for immunostaining. a, b Antigen absorption test. Immunohistochemistry using showing a anti-GLUT9 antibody staining in the third ventricle ependyma and parenchyma and b antigen-preabsorbed antibody. Scale bar 100 um. c GLUT9 immunoreactivity observed in brain capillaries in the cortex. Scale bar 100 um. d-f Immunofluorescence staining of d GLUT9 and e P-gp showing co-localization in capillaries. Blue indicates DAPI-stained nucleus of the brain capillary endothelial cell. Scale bar 10 um.Image collected and cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-NBP1-05054-img0007.jpg)
![Western Blot: GLUT9 Antibody [NBP1-05054] - Detection of GLUT9 in human kidney membrane.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Western-Blot-NBP1-05054-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GLUT9 Antibody [NBP1-05054] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-GLUT9 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05054-img0005.jpg)
![Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP1-05054] - IHC-P analysis of mouse kidney section using GLUT9 antibody at 1:200 dilution. This antibody generated an expected cytoplasmic staining in tubular cells with a more staining towards membranes in some cells but no immunopositivity in Bowmans capsules.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-Paraffin-NBP1-05054-img0004.jpg)
![Immunohistochemistry: GLUT9 Antibody - BSA Free [NBP1-05054] - Immunofluorescence staining of GLUT9 in PFA-fixed murine brain sections. Frozen sections of paraformaldehyde-fixed wild-type murine brain were used for immunofluorescence staining. a, b Antigen absorption test. Immunofluorescence staining of the ependymal wall of the dorsal third ventricle using a anti-GLUT9 antibody & b antigen-preabsorbed antibody. Scale bar 100 µm. c, d Immunofluorescence staining of GLUT9 (magenta), acetylated-tubulin (Ac-Tubulin, green) & DAPI (blue) on ependymal cells. Scale bar 10 µm. e–g Immunofluorescence staining of GLUT9 (magenta) & NeuN (green) showing co-localization in neurons. Scale bar 10 µm. D3V, dorsal third ventricle; DAPI, 4′,6-diamidino-2-phenylindole; NeuN, neuronal nucleus marker Image collected & cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05054_rabbit-polyclonal-glut9-antibody-310202416171452.jpg)
![Immunohistochemistry: GLUT9 Antibody - BSA Free [NBP1-05054] - Immunofluorescence staining of ABCG2 in methanol/acetone-fixed & methacarn-fixed murine brain. Fresh frozen sections of the dorsal third ventricle were prepared from wild-type (WT) & ABCG2 knockout (KO) mice & post-fixed with methanol & acetone. Immunofluorescence staining of ABCG2 was seen in choroid plexus & capillaries in sections from a WT but not in b ABCG2 KO mouse. Scale bar 100 µm. c Immunofluorescence staining of ABCG2 (magenta) & GLUT9 (green) showing ABCG2 in the choroid plexus & GLUT9 in the ependyma. Scale bar 100 µm. d–f Immunofluorescence staining of d ABCG2 & e GLUT9 using methacarn-fixed paraffin section of capillaries showing co-localization in f. Blue indicates DAPI-stained nucleus of the brain capillary endothelial cell. Scale bar 10 µm. CP, choroid plexus; D3V, dorsal third ventricle Image collected & cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05054_rabbit-polyclonal-glut9-antibody-310202416173530.jpg)
![Western Blot: PRKAG2 Antibody [NBP1-89324] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize/PRKAG2-Antibody-Western-Blot-NBP1-89324-img0010.jpg "Western Blot: PRKAG2 Antibody [NBP1-89324] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.")
![Immunocytochemistry/Immunofluorescence: PRKAG2 Antibody [NBP1-89324] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PRKAG2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89324-img0009.jpg)
![Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324] - Staining of human prostate shows moderate to strong positivity in nuclear membrane in glandular cells.](http://images.novusbio.com/fullsize/PRKAG2-Antibody-Immunohistochemistry-Paraffin-NBP1-89324-img0014.jpg)
![Western Blot: PRKAG2 Antibody [NBP1-89324] - Analysis in human cell line A-431.](http://images.novusbio.com/fullsize/PRKAG2-Antibody-Western-Blot-NBP1-89324-img0007.jpg)
![Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324] - Staining of human skin shows moderate to strong positivity in nuclear membrane in keratinocytes](http://images.novusbio.com/fullsize/PRKAG2-Antibody-Immunohistochemistry-Paraffin-NBP1-89324-img0011.jpg)
![Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324] - Staining of human kidney shows moderate to strong positivity in nuclear membrane in cells in tubules.](http://images.novusbio.com/fullsize/PRKAG2-Antibody-Immunohistochemistry-Paraffin-NBP1-89324-img0012.jpg)
![Immunohistochemistry-Paraffin: PRKAG2 Antibody [NBP1-89324] - Staining of human testis shows moderate to strong positivity in nuclear membrane in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PRKAG2-Antibody-Immunohistochemistry-Paraffin-NBP1-89324-img0013.jpg)
![Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody (M01), clone 6C7.Lane 1: PIP5K3 transfected lysate(50.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Western-Blot-H00200576-M01-img0017.jpg "Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody (M01), clone 6C7.Lane 1: PIP5K3 transfected lysate(50.2 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of monoclonal antibody to PIP5K3 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Immunocytochemistry-Immunofluorescence-H00200576-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of monoclonal antibody to PIP5K3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Immunohistochemistry-Paraffin-H00200576-M01-img0014.jpg)
![Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 over-expressed 293 cell line, cotransfected with PIP5K3 Validated Chimera RNAi ( Cat # H00200576-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PIP5K3 monoclonal antibody (M01), clone 6C7 (Cat # H00200576-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Western-Blot-H00200576-M01-img0015.jpg)
![Sandwich ELISA: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Detection limit for recombinant GST tagged PIP5K3 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Sandwich-ELISA-H00200576-M01-img0016.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of PI 3 Kinase p85 alpha on different lysates using anti-PI 3 Kinase p85 alpha antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Raji](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Western-Blot-NBP2-67488-img0010.jpg "Western Blot: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of PI 3 Kinase p85 alpha on different lysates using anti-PI 3 Kinase p85 alpha antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Raji")
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Staining PI 3 Kinase p85 alpha in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunocytochemistry-Immunofluorescence-NBP2-67488-img0005.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of paraffin-embedded mouse kidney tissue using anti-PI 3 Kinase p85 alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunohistochemistry-Paraffin-NBP2-67488-img0009.jpg)
![Flow Cytometry: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of HepG2 cells with PI 3 Kinase p85 alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antib](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Flow-Cytometry-NBP2-67488-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Staining PI 3 Kinase p85 alpha in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunocytochemistry-Immunofluorescence-NBP2-67488-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Staining PI 3 Kinase p85 alpha in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunocytochemistry-Immunofluorescence-NBP2-67488-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Staining PI 3 Kinase p85 alpha in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunocytochemistry-Immunofluorescence-NBP2-67488-img0004.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of paraffin-embedded human kidney tissue using anti-PI 3 Kinase p85 alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunohistochemistry-Paraffin-NBP2-67488-img0006.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of paraffin-embedded human liver cancer tissue using anti-PI 3 Kinase p85 alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunohistochemistry-Paraffin-NBP2-67488-img0007.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (SU04-07) [NBP2-67488] - Analysis of paraffin-embedded mouse heart tissue using anti-PI 3 Kinase p85 alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-SU04-07-Immunohistochemistry-Paraffin-NBP2-67488-img0008.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis on different cell lysates using anti-PI3-kinase p85 subunit alpha Mouse mAb. Positive control: Lane 1: U-937 Lane 2: Raji](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Western-Blot-NBP2-67058-img0006.jpg "Western Blot: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis on different cell lysates using anti-PI3-kinase p85 subunit alpha Mouse mAb. Positive control: Lane 1: U-937 Lane 2: Raji")
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Staining of MCF-7 cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunocytochemistry-Immunofluorescence-NBP2-67058-img0008.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis of paraffin- embedded mouse skeletal muscle tissue using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunohistochemistry-Paraffin-NBP2-67058-img0009.jpg)
![Flow Cytometry: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis of MCF-7 cells with PI3-kinase p85 subunit alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondar](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Flow-Cytometry-NBP2-67058-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Staining of Hela cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunocytochemistry-Immunofluorescence-NBP2-67058-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Staining of SH-SY-5Y cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunocytochemistry-Immunofluorescence-NBP2-67058-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Staining of PANC-1 cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunocytochemistry-Immunofluorescence-NBP2-67058-img0007.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis of paraffin- embedded human breast carcinoma tissue using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunohistochemistry-Paraffin-NBP2-67058-img0004.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (A3-D0) [NBP2-67058] - Analysis of paraffin- embedded human colon cancer tissue using anti-PI3-kinase p85 subunit alpha Mouse mAb.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-A3-D0-Immunohistochemistry-Paraffin-NBP2-67058-img0005.jpg)
![Western Blot: HADH Antibody (4B5) [H00003033-M01] - Analysis of HADH expression in transfected 293T cell line by HADHSC monoclonal antibody (M01), clone 4B5.Lane 1: HADH transfected lysate(34.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HADH-Antibody-4B5-Western-Blot-H00003033-M01-img0012.jpg "Western Blot: HADH Antibody (4B5) [H00003033-M01] - Analysis of HADH expression in transfected 293T cell line by HADHSC monoclonal antibody (M01), clone 4B5.Lane 1: HADH transfected lysate(34.3 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: HADH Antibody (4B5) [H00003033-M01] - Analysis of monoclonal antibody to HADHSC on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/HADH-Antibody-4B5-Immunocytochemistry-Immunofluorescence-H00003033-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: HADH Antibody (4B5) [H00003033-M01] - Analysis of monoclonal antibody to HADHSC on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/HADH-Antibody-4B5-Immunohistochemistry-Paraffin-H00003033-M01-img0008.jpg)
![Western Blot: HADH Antibody (4B5) [H00003033-M01] - HADHSC monoclonal antibody (M01), clone 4B5 Analysis of HADHSC expression in HepG2.](http://images.novusbio.com/fullsize/HADH-Antibody-4B5-Western-Blot-H00003033-M01-img0011.jpg)
![Immunoprecipitation: HADH Antibody (4B5) [H00003033-M01] - Analysis of HADH transfected lysate using anti-HADH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HADH MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/HADH-Antibody-4B5-Immunoprecipitation-H00003033-M01-img0009.jpg)
![ELISA: HADH Antibody (4B5) [H00003033-M01] - Detection limit for recombinant GST tagged HADHSC is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HADH-Antibody-4B5-ELISA-H00003033-M01-img0010.jpg)
![Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - Western blot analysis using Glucose Transporter GLUT4 mouse mAb against HeLa (1), NIH3T3 (2), 3T3-L1 (3) cell lysate and Mouse heart (4) tissue lysate.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Western-Blot-NBP2-22214-img0008.jpg "Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - Western blot analysis using Glucose Transporter GLUT4 mouse mAb against HeLa (1), NIH3T3 (2), 3T3-L1 (3) cell lysate and Mouse heart (4) tissue lysate.")
![Immunocytochemistry/Immunofluorescence: Glut4 Antibody (3G10A3) [NBP2-22214] - Analysis of HepG2 cells using Glucose Transporter GLUT4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Immunocytochemistry-Immunofluorescence-NBP2-22214-img0003.jpg)
![Immunohistochemistry-Paraffin: Glut4 Antibody (3G10A3) [NBP2-22214] - analysis of cardiac muscle tissues using SLC2A4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Immunohistochemistry-Paraffin-NBP2-22214-img0009.jpg)
![Flow Cytometry: Glut4 Antibody (3G10A3) [NBP2-22214] - Flow cytometric analysis of HeLa cells using Glucose Transporter GLUT4 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Flow-Cytometry-NBP2-22214-img0001.jpg)
![Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - Western blot analysis using Glucose Transporter Glut4 Antibody (3G10A3)mAb against HEK293 (1) and Glucose Transporter GLUT4 (AA: 224-353)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Western-Blot-NBP2-22214-img0006.jpg)
![Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - human Glucose Transporter GLUT4 recombinant protein.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Western-Blot-NBP2-22214-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Glut4 Antibody (3G10A3) [NBP2-22214] - Analysis of HeLa cells using Glucose Transporter GLUT4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Immunocytochemistry-Immunofluorescence-NBP2-22214-img0002.jpg)
![Immunohistochemistry-Paraffin: Glut4 Antibody (3G10A3) [NBP2-22214] - Analysis of paraffin-embedded bladder cancer tissues using Glucose Transporter GLUT4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-Immunohistochemistry-Paraffin-NBP2-22214-img0004.jpg)
![ELISA: Glut4 Antibody (3G10A3) [NBP2-22214] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/Glut4-Antibody-3G10A3-ELISA-NBP2-22214-img0005.jpg)
![mTORC2 in adipose tissue is required for cold‐induced glucose uptake and glycolysis2‐deoxyglucose‐6‐phosphate (2DG6P) accumulation in BAT of AdRiKO and control mice housed at 22 or at 4C for 4 h (n = 6/group).Extracellular acidification rate (ECAR) of BAT explants from AdRiKO and control mice housed at 22 or at 4C for 4 h (n = 7/group).Immunoblot analysis of BAT from AdRiKO and control mice housed at 22 or at 4C for 8 h for the indicated proteins (n = 6/group, each lane represents a mix of 3 mice).Immunoblot analysis of isolated plasma membranes from BAT of AdRiKO and control mice housed at 22 or at 4C for 8 h for the indicated proteins (n = 6/group, each lane represents a mix of 3 mice).Immunoblot analysis of mitochondrial and cytosolic fractions from BAT of AdRiKO and control mice housed at 22 or at 4C for 4 h for the indicated proteins (n = 6/group, each lane represents a mix of 3 mice).Cytosolic hexokinase activity in BAT of AdRiKO and control mice housed at 22 or at 4C for 4 h [n = 5 (control 22C), n = 5 (AdRiKO 22C), n = 7 (control 4C), n = 7 (AdRiKO 4C)].Mitochondrial hexokinase activity in BAT of AdRiKO and control mice housed at 22 or at 4C for 4 h [n = 5 (control 22C), n = 5 (AdRiKO 22C), n = 7 (control 4C), n = 7 (AdRiKO 4C)].Data information: Data represent mean +/- SEM. Statistically significant differences between AdRiKO and control mice were determined with unpaired Student's t‐test and are indicated with asterisks (*P < 0.05). Statistically significant differences between temperatures were determined with unpaired Student's t‐test and are indicated with a number sign (#P < 0.05; ##P < 0.01; ###P < 0.001). The exact P‐value for each significant difference can be found in Appendix Table S2. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26772600), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-22214_mouse-monoclonal-glut4-antibody-3g10a3-western-blot-132202615255419.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis using PI 3 Kinase p85 alpha mAb against human PI 3 Kinase p85 alpha recombinant protein. (Expected MW is 53.4 kDa)](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-Western-Blot-NBP2-22212-img0007.jpg "Western Blot: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis using PI 3 Kinase p85 alpha mAb against human PI 3 Kinase p85 alpha recombinant protein. (Expected MW is 53.4 kDa)")
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of HepG2 cells using PI 3 Kinase p85 alpha mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-Immunocytochemistry-NBP2-22212-img0009.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of rectum cancer tissues using PI 3 Kinase p85 alpha mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-Immunohistochemistry-Paraffin-NBP2-22212-img0005.jpg)
![Flow Cytometry: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of NIH3T3 cells using PI 3 Kinase p85 alpha mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-Flow-Cytometry-NBP2-22212-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of HeLa cells using PI 3 Kinase p85 alpha mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-Immunocytochemistry-NBP2-22212-img0008.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Analysis of cervical cancer tissues using PI 3 Kinase p85 alpha mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-Immunohistochemistry-Paraffin-NBP2-22212-img0004.jpg)
![ELISA: PI 3-Kinase p85 alpha Antibody (6G10) [NBP2-22212] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-6G10-ELISA-NBP2-22212-img0006.jpg)