| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-128SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-127SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71706SS | |||
| Azide and BSA Free | NBP2-80560 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-117SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-110SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70068 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-89182SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89526-25ul | |||
![Western Blot: Niemann-Pick type C1 Like-1 Antibody [NB400-128] - Detection of NPC3 in rat small intestine membrane preparations (20 ug). (1) 2 ug/ml and (2) 4 ug/ml. ECL: 30 minute exposure.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Western-Blot-NB400-128-img0004.jpg "Western Blot: Niemann-Pick type C1 Like-1 Antibody [NB400-128] - Detection of NPC3 in rat small intestine membrane preparations (20 ug). (1) 2 ug/ml and (2) 4 ug/ml. ECL: 30 minute exposure.")
![Immunohistochemistry-Paraffin: Niemann-Pick type C1 Like-1 Antibody [NB400-128] - Staining of human small intestine, enterocytes.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunohistochemistry-Paraffin-NB400-128-img0005.jpg)
![Western Blot: Niemann-Pick type C1 Like-1 Antibody [NB400-128] - Decrease in the biliary cholesterol secretion in L1-Tg mice. Immunoblot detection of hepatic NPC1L1 protein in L1-Tg mice using an anti-NPC1L1 (Niemann-Pick type C1 Like-1) antibody. WT, wild-type. Image collected and cropped by CiteAb from the following publication (//lipidworld.biomedcentral.com/articles/10.1186/s12944-019-1179-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Western-Blot-NB400-128-img0006.jpg)
![Western Blot: Niemann-Pick type C1 Like-1 Antibody - BSA Free [NB400-128] - Hepatic NPC1L1‐mediated steatosis in the liver of L1‐Tg mice. A, Hepatic expression of the human NPC1L1 protein in L1‐Tg mice demonstrated by immunoblotting using an anti‐NPC1L1 antibody. alpha ‐Tubulin, a loading control. B, Decrease of biliary cholesterol levels in L1‐Tg mice. Data are expressed as the mean ± SEM n = 6 (WT) & 7 (L1‐Tg). C, Photographic images of the livers of WT & L1‐Tg mice fed a control fat diet (CFD) or a high‐fat diet (HFD) for 2 weeks. The coin diameter was 1 cm. D, Hematoxylin & eosin (H&E) & Oil Red O staining of the livers of WT & L1‐Tg mice fed a HFD for 2 weeks. E & F, Hepatic cholesterol levels (E, left), hepatic triglyceride (TG) levels (E, right), body weight (BW) (F, left), & the ratios of liver weight to BW (L/B ratio) (F, right) in each group of mice fed a CFD or HFD for 2 weeks. Data are expressed as the mean ± SEM n = 11 (WT‐CFD) & 7 (the other groups). Statistical analyses for significant differences were performed using Bartlett's test, followed by a parametric Tukey–Kramer multiple‐comparison test (E, right; F) or a non‐parametric Steel–Dwass test (E, left) (##P < 0.01 vs CFD controls; **P < 0.01 vs. the other groups; *P < 0.05 among two groups; NS, not significantly different among groups) as well as a two‐sided t test (††P < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32123832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-128_rabbit-polyclonal-niemann-pick-type-c1-like-1-antibody-310202416165636.jpg)
![Western Blot: Niemann-Pick type C1 Like-1 Antibody [NB400-127] - Detection of NPC1L1 protein in lysate of human small intestine tissue using Niemann-Pick type C1 Like-1 antibody at 2ug/ml concentration.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Western-Blot-NB400-127-img0007.jpg "Western Blot: Niemann-Pick type C1 Like-1 Antibody [NB400-127] - Detection of NPC1L1 protein in lysate of human small intestine tissue using Niemann-Pick type C1 Like-1 antibody at 2ug/ml concentration.")
![Immunocytochemistry/Immunofluorescence: Niemann-Pick type C1 Like-1 Antibody [NB400-127] - HepG2 cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Niemann-Pick type C1 Like-1 Antibody (NB400-127) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunocytochemistry-Immunofluorescence-NB400-127-img0008.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick type C1 Like-1 Antibody [NB400-127] - Analysis of a FFPE tissue section of mouse small intestine using 1:200 dilution of NPC1L1 antibody (NB400-127). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunohistochemistry-Paraffin-NB400-127-img0009.jpg)
![Western Blot: ABCG8 Antibody (1B10A5) [NBP1-71706] - Analysis of ABCG8 expression in ABCG8 lysate using NBP1-71706.](http://images.novusbio.com/fullsize/ABCG8-Antibody-1B10A5-Western-Blot-NBP1-71706-img0006.jpg "Western Blot: ABCG8 Antibody (1B10A5) [NBP1-71706] - Analysis of ABCG8 expression in ABCG8 lysate using NBP1-71706.")
![Immunocytochemistry/Immunofluorescence: ABCG8 Antibody (1B10A5) [NBP1-71706] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.1% Saponin. The cells were incubated with anti-ABCG8 Antibody (1B10A5) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ABCG8-Antibody-1B10A5-Immunocytochemistry-Immunofluorescence-NBP1-71706-img0007.jpg)
![Immunohistochemistry: ABCG8 Antibody (1B10A5) [NBP1-71706] - Immunohistochemical analysis of ABCG8 in mouse intestine using NBP1-71706.](http://images.novusbio.com/fullsize/ABCG8-Antibody-1B10A5-Immunohistochemistry-NBP1-71706-img0005.jpg)
![Western Blot: ABCG8 Antibody [NB400-117] - Total protein from Mouse small intestine was separated on a 7.5% gel by SDS-PAGE and transferred to PVDF membrane. The membrane was then probed with anti-ABCG8 antibody at 2 ug/ml and detected with an anti-rabbit HRP conjugated secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/ABCG8-Antibody-Western-Blot-NB400-117-img0003.jpg "Western Blot: ABCG8 Antibody [NB400-117] - Total protein from Mouse small intestine was separated on a 7.5% gel by SDS-PAGE and transferred to PVDF membrane. The membrane was then probed with anti-ABCG8 antibody at 2 ug/ml and detected with an anti-rabbit HRP conjugated secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: ABCG8 Antibody [NB400-117] - ABCG8 antibody was tested in HEK293 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/ABCG8-Antibody-Immunocytochemistry-Immunofluorescence-NB400-117-img0002.jpg)
![Western Blot: ABCG8 Antibody [NB400-110] - Analysis of ABCG8, using NB400-110. Samples: mouse liver microsomes from G5/G8 knockout mice, wildtype human G5/G8, and transgenic human G5/G8.](http://images.novusbio.com/fullsize/ABCG8-Antibody-Western-Blot-NB400-110-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ABCG8 Antibody [NB400-110] - Analysis of ABCG8, using NB400-110. Samples: mouse liver microsomes from G5/G8 knockout mice, wildtype human G5/G8, and transgenic human G5/G8.")
![Western Blot: ACAT2 Antibody (3E2) [NBP2-02588] Analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Western-Blot-NBP2-02588-img0008.jpg "Western Blot: ACAT2 Antibody (3E2) [NBP2-02588] Analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: ACAT2 Antibody (3E2) [NBP2-02588] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAT2.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Immunocytochemistry-Immunofluorescence-NBP2-02588-img0007.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody (3E2) [NBP2-02588] - Staining of paraffin-embedded Human prostate tissue using anti-ACAT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Immunohistochemistry-Paraffin-NBP2-02588-img0006.jpg)
![Flow Cytometry: ACAT2 Antibody (3E2) [NBP2-02588] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACAT2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Flow-Cytometry-NBP2-02588-img0002.jpg)
![Western Blot: ACAT2 Antibody (3E2) [NBP2-02588] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Western-Blot-NBP2-02588-img0003.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody (3E2) [NBP2-02588] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ACAT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Immunohistochemistry-Paraffin-NBP2-02588-img0004.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody (3E2) [NBP2-02588] - Staining of paraffin-embedded Human liver tissue using anti-ACAT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Immunohistochemistry-Paraffin-NBP2-02588-img0005.jpg)
![Flow Cytometry: ACAT2 Antibody (3E2) [NBP2-02588] - Analysis of Jurkat cells, using anti-ACAT2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ACAT2-Antibody-3E2-Flow-Cytometry-NBP2-02588-img0001.jpg)
![Western Blot: Apolipoprotein B/ApoB Antibody [NB200-527] - Alcohol feeding significantly down-regulated ApoB expression in mouse liver. Image from verified customer review.](http://images.novusbio.com/fullsize/Apolipoprotein-B-ApoB-Antibody-Western-Blot-NB200-527-img0001.jpg "Western Blot: Apolipoprotein B/ApoB Antibody [NB200-527] - Alcohol feeding significantly down-regulated ApoB expression in mouse liver. Image from verified customer review.")
![Western Blot: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Total protein from rat liver was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1L1 (NBP2-89182) in 1% BSA-TBST buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Western-Blot-NBP2-89182-img0003.jpg "Western Blot: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Total protein from rat liver was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1L1 (NBP2-89182) in 1% BSA-TBST buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Niemann-Pick type C1 Like-1 Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-89182-img0002.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick type C1 Like-1 Antibody [NBP2-89182] - Analysis of a FFPE tissue section of human liver using 1:200 dilution of NPC1L1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This NPC1L1 antibody generated moderate staining of hepatocyte cytoplasm.](http://images.novusbio.com/fullsize/Niemann-Pick-type-C1-Like-1-Antibody-Immunohistochemistry-Paraffin-NBP2-89182-img0001.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining in human liver and skeletal muscle tissues using NBP1-89525 antibody. Corresponding ACAT2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89525-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining in human liver and skeletal muscle tissues using NBP1-89525 antibody. Corresponding ACAT2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining of human gastrointestinal, liver, skeletal muscle and testis using Anti-ACAT2 antibody NBP1-89525 (A) shows similar protein distribution across tissues to independent antibody NBP1-89526 (B).](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89525-img0016.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ACAT2 Antibody [NBP1-89525] - Analysis using Anti-ACAT2 antibody NBP1-89525 (A) shows similar pattern to independent antibody NBP1-85967 (B).](http://images.novusbio.com/fullsize/ACAT2-Antibody-Western-Blot-NBP1-89525-img0011.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89525-img0017.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89525-img0018.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89525-img0019.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89525-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: ACAT2 Antibody [NBP1-89526] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89526-img0006.jpg "Immunocytochemistry/Immunofluorescence: ACAT2 Antibody [NBP1-89526] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89526] - Staining of human gastrointestinal, liver, skeletal muscle and testis using Anti-ACAT2 antibody NBP1-89526 (A) shows similar protein distribution across tissues to independent antibody NBP1-89525 (B).](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89526-img0012.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89526] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89526-img0013.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89526] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89526-img0015.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89526] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89526-img0014.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89526] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-89526-img0016.jpg)
![Western Blot: Leptin/OB Antibody [NBP1-59324] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.](http://images.novusbio.com/fullsize/Leptin-OB-Antibody-Western-Blot-NBP1-59324-img0006.jpg "Western Blot: Leptin/OB Antibody [NBP1-59324] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.")
![Immunohistochemistry-Paraffin: Leptin/OB Antibody [NBP1-59324] - Human pancreas tissue, 4-8ug/ml.](http://images.novusbio.com/fullsize/Leptin-OB-Antibody-Immunohistochemistry-Paraffin-NBP1-59324-img0005.jpg)
![Simple Western: Leptin/OB Antibody [NBP1-59324] - Simple Western analysis of Leptin. Lane 1- biotinylated ladder; Lane 2- negative control (non-transfected MIN6 cells, 0.2 mg/ml); Lane 3- MIN6 cells transfected with plasmid expressing mouse leptin (positive control, 0.2 mg/ml). Image from verified customer review.](http://images.novusbio.com/fullsize/Leptin-OB-Antibody-Simple-Western-NBP1-59324-img0007.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-85967] - Analysis in human liver and skeletal muscle tissues using NBP1-85967 antibody. Corresponding ACAT2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-85967-img0023.jpg "Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-85967] - Analysis in human liver and skeletal muscle tissues using NBP1-85967 antibody. Corresponding ACAT2 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-85967] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-85967-img0016.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-85967] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-85967-img0017.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-85967] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-85967-img0018.jpg)
![Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-85967] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/ACAT2-Antibody-Immunohistochemistry-Paraffin-NBP1-85967-img0019.jpg)
