| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2383SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77680SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76798SS | |||
| Azide and BSA Free | NBP2-80879 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73152 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90331-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59320-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-55454SS | |||
| Azide and BSA Free | NBP2-80580 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82572-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80992-25ul | |||
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse placenta tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0006.jpg "Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse placenta tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.")
![Western Blot: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of LAMP2a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human liver tissue lysateLane 2: JAR cell lysate Lane 3: human placenta tissue lysate Lane 4: human lung tissue lysate](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Western-Blot-NBP2-67298-img0007.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-LAMP-2/CD107b antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0008.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human kidney tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0001.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human liver tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0002.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded human pancreas tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0003.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse brain tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0004.jpg)
![Immunohistochemistry-Paraffin: LAMP-2/CD107b Antibody (SA46-01) [NBP2-67298] - Analysis of paraffin-embedded mouse kidney tissue using anti-LAMP-2/CD107b antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/LAMP-2-CD107b-Antibody-SA46-01-Immunohistochemistry-Paraffin-NBP2-67298-img0005.jpg)
![Western Blot: PDK-1 Antibody [NB100-2383] - Analysis of HeLa lysates using NB100-2383. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).](http://images.novusbio.com/fullsize/PDK-1-Antibody-Western-Blot-NB100-2383-img0009.jpg "Western Blot: PDK-1 Antibody [NB100-2383] - Analysis of HeLa lysates using NB100-2383. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).")
![Immunocytochemistry/Immunofluorescence: PDK-1 Antibody [NB100-2383] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PDK-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2383-img0007.jpg)
![Western Blot: PDK-1 Antibody [NB100-2383] - Detection of PDK-1 in human heart lysate using NB 100-2383 at 2 ug/ml.](http://images.novusbio.com/fullsize/PDK-1-Antibody-Western-Blot-NB100-2383-img0008.jpg)
![Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGFR1 in: 1. HeLa, 2. NIH/3T3, 3. SK-BR-3 and 4. COS7.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Western-Blot-NBP1-77680-img0009.jpg "Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGFR1 in: 1. HeLa, 2. NIH/3T3, 3. SK-BR-3 and 4. COS7.")
![Immunocytochemistry/Immunofluorescence: IGF-I R Antibody [NBP1-77680] - IGF1 receptor (EC) antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77680-img0008.jpg)
![Immunohistochemistry-Paraffin: IGF-I R Antibody [NBP1-77680] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human kidney cancer xenograft using 1:200 dilution of IFG-I R antibody (NBP1-77680). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Immunohistochemistry-Paraffin-NBP1-77680-img0010.jpg)
![Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGF-I R in human pancreatic cancer cell lines (MiaPaCa-2, AsPC-1, and BxPC-3) using anti-IGF-I R antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Western-Blot-NBP1-77680-img0007.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Knockout-Validated-NBP2-76798-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 for 5 minutes. The cells were incubated with anti- Niemann-Pick C1 Antibody at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0006.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Total protein from human HeLa, Hek293, mouse 3T3 and Rat PC12 cell lines were separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Western-Blot-NBP2-76798-img0005.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Niemann-Pick Type C1/NPC1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (NBP2-76798) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic puncta.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunohistochemistry-Paraffin-NBP2-76798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Niemann-Pick C1 Antibody (1318A) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0004.jpg)
![Western Blot: OSBP1 Antibody (1F2) [NBP2-00935] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBP1.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Western-Blot-NBP2-00935-img0002.jpg "Western Blot: OSBP1 Antibody (1F2) [NBP2-00935] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBP1.")
![Immunocytochemistry/Immunofluorescence: OSBP1 Antibody (1F2) [NBP2-00935] Staining of COS7 cells transiently transfected by pCMV6-ENTRY OSBP1.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunocytochemistry-Immunofluorescence-NBP2-00935-img0014.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (OTI1F2) [NBP2-00935] - Staining of paraffinembedded Adenocarcinoma of Humanendometrium tissue using anti-OSBP mousemonoclonal antibody. (Heat-induced epitoperetrieval by 10mM citric buffer, pH6.0, 100 degrees C for10min, TA503140)](http://images.novusbio.com/fullsize/OSBP1-Antibody-OTI1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0015.jpg)
![Flow Cytometry: OSBP1 Antibody (1F2) [NBP2-00935] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-OSBP1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Flow-Cytometry-NBP2-00935-img0001.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0003.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0004.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0005.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0006.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0007.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0008.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0009.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Human endometrium tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0010.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Human Kidney tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0011.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Human pancreas tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0012.jpg)
![Immunohistochemistry-Paraffin: OSBP1 Antibody (1F2) [NBP2-00935] - Staining of paraffin-embedded Human prostate tissue using anti-OSBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/OSBP1-Antibody-1F2-Immunohistochemistry-Paraffin-NBP2-00935-img0013.jpg)
![Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody (M01), clone 6C7.Lane 1: PIP5K3 transfected lysate(50.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Western-Blot-H00200576-M01-img0017.jpg "Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody (M01), clone 6C7.Lane 1: PIP5K3 transfected lysate(50.2 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of monoclonal antibody to PIP5K3 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Immunocytochemistry-Immunofluorescence-H00200576-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of monoclonal antibody to PIP5K3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Immunohistochemistry-Paraffin-H00200576-M01-img0014.jpg)
![Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 over-expressed 293 cell line, cotransfected with PIP5K3 Validated Chimera RNAi ( Cat # H00200576-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PIP5K3 monoclonal antibody (M01), clone 6C7 (Cat # H00200576-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Western-Blot-H00200576-M01-img0015.jpg)
![Sandwich ELISA: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Detection limit for recombinant GST tagged PIP5K3 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Sandwich-ELISA-H00200576-M01-img0016.jpg)
![Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Western-Blot-NBP2-59320-img0007.jpg "Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.")
![Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SUR1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Immunocytochemistry-Immunofluorescence-NBP2-59320-img0008.jpg)
![Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis of mouse pancreatic islets. Lane 1: Molecular Weight Ladder. Lanes 2-5: Samples from pooled pancreatic islets. Load: ~10ug. Primary Antibody: Anti-SUR1 Monoclonal Antibody at 1:200 for ~16hrs at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1hr RT. Image from verified customer review.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Western-Blot-NBP2-59320-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16. Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SUR1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Immunocytochemistry-Immunofluorescence-NBP2-59320-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Glial fibrillary acidic protein (GFAP)-positive specimens from human contusion- traumatic brain injury (TBI) exhibit sulfonylurea receptor 1 (SUR1) expression in astrocytes.Double immunolabeling for SUR1 (red) and GFAP (green) showed astrocyte expression of SUR1; merged images confirm co-localization (yellow); in situ hybridization of the same tissue section for Abcc8 messenger RNA showed positive signal co-localized with GFAP-positive, SUR1-expressing astrocytes; arrowheads point to cells with all three signals. Image collected and cropped by CiteAb from the following publication (//www.liebertpub.com/doi/10.1089/neu.2018.5986) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Immunocytochemistry-Immunofluorescence-NBP2-59320-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Glial fibrillary acidic protein (GFAP)–positive specimens from human contusion- traumatic brain injury (TBI) exhibit KIR6.2 expression in astrocytes. (A) Immunolabeling for KIR6.2 showed sparse immunoreactivity in the control specimen (CTR) vs. widespread expression in a GFAP-positive specimen from contusion-TBI. (B) Double immunolabeling for GFAP (red) & KIR6.2 (green) showed astrocyte expression of KIR6.2; merged images confirm co-localization (yellow); in situ hybridization of the same tissue section for Kcnj11 messenger RNA showed positive signal co-localized with GFAP-positive, KIR6.2-expressing astrocytes; arrowheads point to cells with all three signals. (C) Double immunolabeling showed that KIR6.2 (red) & sulfonylurea receptor 1 (SUR1; green) were co-localized (yellow) in astrocytes. (D) ImmunoFRET for SUR1 (magenta) & KIR6.2 (red) showed co-assembly of SUR1-KIR6.2 heteromers (yellow pseudocolor) in astrocytes. (E) Double immunolabeling showed that KIR6.2 (green) & transient receptor potential cation channel subfamily M member 4 (TRPM4) (red) were co-localized in astrocytes. The findings illustrated are representative of all GFAP-positive specimens from eight cases of human contusion-TBI. (case #2, 11 days post-TBI; case #5, 1 day post-TBI) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30160201), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-59320_mouse-monoclonal-sur1-antibody-s289-16-310202416114773.jpg)
![Western Blot: IPMK Antibody [NBP1-32250] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [N1C2] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IPMK-Antibody-Western-Blot-NBP1-32250-img0010.jpg "Western Blot: IPMK Antibody [NBP1-32250] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [N1C2] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: IPMK Antibody [NBP1-32250] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/IPMK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32250-img0004.jpg)
![Immunohistochemistry-Paraffin: IPMK Antibody [NBP1-32250] - Mouse intestine. IPMK stained by IPMK antibody [N1C2] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/IPMK-Antibody-Immunohistochemistry-Paraffin-NBP1-32250-img0011.jpg)
![Western Blot: IPMK Antibody [NBP1-32250] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IPMK-Antibody-Western-Blot-NBP1-32250-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: IPMK Antibody [NBP1-32250] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [N1C2] diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32250_rabbit-polyclonal-ipmk-antibody-1610202419414528.jpg)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [N1C2] (NBP1-32250) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32250_rabbit-polyclonal-ipmk-antibody-western-blot-1411202518424118.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [N1C2] (NBP1-32250) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32250_rabbit-polyclonal-ipmk-antibody-western-blot-1411202518412924.jpg)
![Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - Analysis in human serum total protein (lane 2). Lane 1 is a MW standard.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Western-Blot-NB110-55454-img0009.jpg "Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - Analysis in human serum total protein (lane 2). Lane 1 is a MW standard.")
![Immunohistochemistry-Paraffin: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human liver using 5 ug/ml conc. of Apolipoprotein A5 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Apolipoprotein A5 in hepatocytes was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Immunohistochemistry-Paraffin-NB110-55454-img0011.jpg)
![Flow Cytometry: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Apolipoprotein A5 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Flow-Cytometry-NB110-55454-img0007.jpg)
![Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - Analysis of ApoA5 in HepG2 lysates](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Western-Blot-NB110-55454-img0002.jpg)
![Western Blot: Apolipoprotein A5 Antibody (1G5G9) [NB110-55454] - Detection of Apo A5 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/Apolipoprotein-A5-Antibody-1G5G9-Western-Blot-NB110-55454-img0008.jpg)
![Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572] - Staining in human kidney and tonsil tissues.. Corresponding PDZK1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PDZK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82572-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572] - Staining in human kidney and tonsil tissues.. Corresponding PDZK1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: PDZK1 Antibody [NBP1-82572] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1, using anti-PDZK1 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/PDZK1-Antibody-Western-Blot-NBP1-82572-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: PDZK1 Antibody [NBP1-82572] - Staining of human cell line RPTEC TERT1 shows localization to nucleus, cytosol & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PDZK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82572-img0015.jpg)
![Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572] - Staining of human tonsil shows no positivity as expected.](http://images.novusbio.com/fullsize/PDZK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82572-img0019.jpg)
![Western Blot: PDZK1 Antibody [NBP1-82572] - Western blot analysis in mouse liver tissue and rat liver tissue.](http://images.novusbio.com/fullsize/PDZK1-Antibody-Western-Blot-NBP1-82572-img0009.jpg)
![Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572] - Staining of human pancreas shows weak to moderate positivity in plasma membrane in islets of Langerhans.](http://images.novusbio.com/fullsize/PDZK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82572-img0016.jpg)
![Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572] - Staining of human small intestine shows moderate to strong positivity in luminal membrane in glandular cells.](http://images.novusbio.com/fullsize/PDZK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82572-img0017.jpg)
![Immunohistochemistry-Paraffin: PDZK1 Antibody [NBP1-82572] - Staining of human kidney shows moderate to strong positivity in apical membrane in cells in proximal tubules.](http://images.novusbio.com/fullsize/PDZK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82572-img0020.jpg)
![Western Blot: NME1-NME2 Antibody [NBP1-80992] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize/NME1-NME2-Antibody-Western-Blot-NBP1-80992-img0011.jpg "Western Blot: NME1-NME2 Antibody [NBP1-80992] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.")
![Immunocytochemistry/Immunofluorescence: NME1-NME2 Antibody [NBP1-80992] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/NME1-NME2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80992-img0010.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody [NBP1-80992] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myoctes.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-Immunohistochemistry-Paraffin-NBP1-80992-img0014.jpg)
![Western Blot: NME1-NME2 Antibody [NBP1-80992] - Analysis in human cell line A-549.](http://images.novusbio.com/fullsize/NME1-NME2-Antibody-Western-Blot-NBP1-80992-img0007.jpg)
![Immunohistochemistry-Paraffin: NME1-NME2 Antibody [NBP1-80992] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/NME1-NME2-Antibody-Immunohistochemistry-Paraffin-NBP1-80992-img0009.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody [NBP1-80992] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-Immunohistochemistry-Paraffin-NBP1-80992-img0012.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody [NBP1-80992] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-Immunohistochemistry-Paraffin-NBP1-80992-img0013.jpg)