| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87490-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70169 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70204 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05769SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2911SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-99585SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87455-25ul | |||
![Simple Western: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Simple Western lane view shows a specific band for ARG1 in 0.2 mg/ml of Liver (left), HepG2 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Simple-Western-NBP1-87490-img0008.jpg "Simple Western: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Simple Western lane view shows a specific band for ARG1 in 0.2 mg/ml of Liver (left), HepG2 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining in human liver and kidney tissues . Corresponding ARG1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87490-img0019.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Analysis using Anti-ARG1 antibody NBP1-87490 (A) shows similar pattern to independent antibody NBP1-87455 (B).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Western-Blot-NBP1-87490-img0020.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining of human bone marrow shows strong cytoplasmic and nuclear positivity in a subset of hematopoietic cells.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87490-img0015.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining of human hepatocellular carcinoma shows moderate cytoplasmic and nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87490-img0016.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87490-img0017.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87490-img0018.jpg)
![Simple Western: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Electropherogram image(s) of corresponding Simple Western lane view. Arginase 1/ARG1/liver Arginase antibody was used at 1:50 dilution on Liver and HepG2 lysate(s).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Simple-Western-NBP1-87490-img0009.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ALOX15 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Western-Blot-NBP2-01740-img0012.jpg "Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ALOX15 monoclonal antibody.(1:200)")
![Immunocytochemistry/Immunofluorescence: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - COS7 cells](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunocytochemistry-Immunofluorescence-NBP2-01740-img0011.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0008.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15 Lipoxygenase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15 Lipoxygenase 1.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Western-Blot-NBP2-01740-img0001.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Analysis of extracts (35ug) from 9 different cell lines by using anti-15 Lipoxygenase 1 monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Western-Blot-NBP2-01740-img0009.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0002.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0003.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0004.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0005.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0006.jpg)
![Immunohistochemistry-Paraffin: 15-Lipoxygenase 1 Antibody (OTI3G8) [NBP2-01740] - Staining of paraffin-embedded Human breast tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-3G8-Immunohistochemistry-Paraffin-NBP2-01740-img0007.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using antiARG1 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-4E6-Western-Blot-NBP2-03618-img0009.jpg "Western Blot: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using antiARG1 monoclonal antibody.(1:200)")
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - Staining of paraffin-embedded Human liver tissue using anti-liver Arginase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-4E6-Immunohistochemistry-Paraffin-NBP2-03618-img0006.jpg)
![Flow Cytometry: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-liver Arginase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-4E6-Flow-Cytometry-NBP2-03618-img0005.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY liver Arginase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-liver Arginase.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-4E6-Western-Blot-NBP2-03618-img0007.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody (4E6) [NBP2-03618] - Analysis of extracts (35ug) from 9 different cell lines by using anti-liver Arginase monoclonal antibody.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-4E6-Western-Blot-NBP2-03618-img0008.jpg)
![Immunohistochemistry-Paraffin: DGAT2 Antibody [NB100-57851] - Analysis of DGAT2 in Human Liver. Antibody at 3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/DGAT2-Antibody-Immunohistochemistry-NB100-57851-img0001.jpg "Immunohistochemistry-Paraffin: DGAT2 Antibody [NB100-57851] - Analysis of DGAT2 in Human Liver. Antibody at 3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.")
![Flow Cytometry: DGAT2 Antibody [NB100-57851] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/DGAT2-Antibody-Flow-Cytometry-NB100-57851-img0006.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-54621] - Jurkat cell lysate, concentration 5.0ug/ml.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Western-Blot-NBP1-54621-img0002.jpg "Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-54621] - Jurkat cell lysate, concentration 5.0ug/ml.")
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-54621] - Human Lung Alveolar cells (indicated with arrows), 4-8ug/ml.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-54621-img0003.jpg)
![Immunohistochemistry: Arginase 1/ARG1/liver Arginase Antibody [NBP1-54621] - Genetic deletion of IL-6 results in less fibrosis and increased remodeling at a chronic time point. A) Anti-Arginase 1/ARG1/liver Arginase immunohistochemistry staining results (n = 4-5; ∗P = .0210) and representative slides. The number of Arg1+ cells, a marker for prohealing monocytes and macrophages, was less in the IL-6-/- model. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35647566/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-54621_rabbit-polyclonal-arginase-1-arg1-liver-arginase-antibody-20920231412575.jpg)
![Immunocytochemistry/Immunofluorescence: DGAT2 Antibody [NBP3-05769] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-DGAT2 Antibody NBP3-05769 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/DGAT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05769-img0005.jpg "Immunocytochemistry/Immunofluorescence: DGAT2 Antibody [NBP3-05769] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-DGAT2 Antibody NBP3-05769 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry-Paraffin: DGAT2 Antibody [NBP3-05769] - Analysis of a FFPE tissue section of human liver using 1:200 dilution of DGAT2, catalog number NBP3-05769 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Cytoplasmic staing is observed.](http://images.novusbio.com/fullsize/DGAT2-Antibody-Immunohistochemistry-Paraffin-NBP3-05769-img0001.jpg)
![Flow (Intracellular): DGAT2 Antibody [NBP3-05769] - An intracellular stain was performed on NIH3T3 cells with DGAT2 Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/DGAT2-Antibody-Flow-Intracellular-NBP3-05769-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: DGAT2 Antibody [NBP3-05769] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-DGAT2 Antibody NBP3-05769 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/DGAT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05769-img0004.jpg)
![Flow (Intracellular): DGAT2 Antibody [NBP3-05769] - An intracellular stain was performed on HepG2 cells with DGAT2 Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/DGAT2-Antibody-Flow-Intracellular-NBP3-05769-img0002.jpg)
![Western Blot: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of Iba1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Western-Blot-NBP2-75397-img0008.jpg "Western Blot: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of Iba1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Staining AIF-1/Iba1 in SH-SY5Y cells using (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunocytochemistry-Immunofluorescence-NBP2-75397-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Iba1 with Rabbit anti-AIF-1/Iba1 antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0015.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of THP-1 cells withAIF-1:Iba1 Antibody (JM36-62) at 1:100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Flow-Cytometry-NBP2-75397-img0001.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human lung cancer tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0003.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human spleen tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0004.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse brain tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0005.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse spleen tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0006.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded rat lung tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0007.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence staining of paraffin- embedded human spleen tissue using anti-AIF-1/Iba1rabbit polyclonal antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/50 dilution for 10 hours at 4C and detected using Alexa Fluor(R) 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0009.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence staining of paraffin- embedded mouse brain tissue using anti-AIF-1/Iba1 antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/50 dilution for 10 hours at 4 and detected using Alexa Fluor(R) 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0010.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0011.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0012.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human spleen tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0013.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0014.jpg)
![Western Blot: Ceramide Kinase Antibody [NB100-2911] - Detection of Ceramide Kinase in A549 cell lysate using NB100-2911.](http://images.novusbio.com/fullsize/Ceramide-Kinase-Antibody-Western-Blot-NB100-2911-img0003.jpg "Western Blot: Ceramide Kinase Antibody [NB100-2911] - Detection of Ceramide Kinase in A549 cell lysate using NB100-2911.")
![Immunocytochemistry/Immunofluorescence: Ceramide Kinase Antibody [NB100-2911] - Ceramide Kinase antibody was tested in A431 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Ceramide-Kinase-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2911-img0004.jpg)
![Western Blot: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Western-Blot-NBP3-13648-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Rat brain. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - AIF-1/Iba1 antibody [HL22] detects AIF-1/Iba1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse spinal cord. Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:200. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Frozen-NBP3-13648-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Mouse hippocampus. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Rat cerebellum. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0003.jpg)
![Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - AIF-1/Iba1 antibody [HL22] detects AIF-1/Iba1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse brain. Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:100. Red: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22-RT] diluted at 1:100. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Frozen-NBP3-13648-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody [HL22] diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-immunocytochemistry-immunofluorescence-244202411033..jpg)
![Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Iba1 stained by Iba1 antibody [HL22] (NBP3-13648) conjugated with DyLight488 diluted at 1:200.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-immunohistochemistry-paraffin-261120251850420.jpg)
![Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-western-blot-2611202518454020.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15 Lipoxygenase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15 L](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Western-Blot-NBP2-70169-img0004.jpg "Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15 Lipoxygenase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15 L")
![Immunocytochemistry/Immunofluorescence: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining in COS7 cells](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70169-img0005.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Human breast tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0011.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ALOX15 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Western-Blot-NBP2-70169-img0001.jpg)
![Western Blot: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Analysis of extracts (35ug) from 9 different cell lines by using anti-15 Lipoxygenase 1 monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Western-Blot-NBP2-70169-img0002.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0006.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0007.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0008.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0009.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0010.jpg)
![Immunohistochemistry: 15-Lipoxygenase 1 Antibody (OTI3G8) - Azide and BSA Free [NBP2-70169] - Staining of paraffin-embedded Human endometrium tissue using anti-15 Lipoxygenase 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/15-Lipoxygenase-1-Antibody-OTI3G8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70169-img0012.jpg)
![Western Blot: ACSL1 Antibody [NB110-99585] - Detection of ACSL in HepG2 whole cell lysates.](http://images.novusbio.com/fullsize/ACSL1-Antibody-Western-Blot-NB110-99585-img0002.jpg "Western Blot: ACSL1 Antibody [NB110-99585] - Detection of ACSL in HepG2 whole cell lysates.")
![Immunocytochemistry/Immunofluorescence: ACSL1 Antibody [NB110-99585] - ACSL1 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/ACSL1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-99585-img0004.jpg)
![Simple Western: ACSL1 Antibody [NB110-99585] - Simple Western lane view shows a specific band for ACSL1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ACSL1-Antibody-Simple-Western-NB110-99585-img0005.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Analysis using Anti-ARG1 antibody NBP1-87455 (A) shows similar pattern to independent antibody NBP1-87490 (B).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Western-Blot-NBP1-87455-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Analysis using Anti-ARG1 antibody NBP1-87455 (A) shows similar pattern to independent antibody NBP1-87490 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Staining in human liver and pancreas tissues using anti-ARG1 antibody. Corresponding ARG1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87455-img0009.jpg)
![Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Western-Blot-NBP1-87455-img0005.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Staining of human liver shows high expression.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87455-img0007.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87455] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87455-img0008.jpg)