| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32932-0.02mg | |||
| Azide and BSA Free | NBP2-34713-0.2mg | |||
| Azide and BSA Free | NBP2-34713-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | BC100-520SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76963-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-481SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-30328SS | |||
| Azide and BSA Free | NBP2-80712 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-22530-50ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48484 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-519SS | |||
![Immunocytochemistry/Immunofluorescence: FLAP Antibody [NB300-891] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong endplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/FLAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-891-img0006.jpg "Immunocytochemistry/Immunofluorescence: FLAP Antibody [NB300-891] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong endplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry-Paraffin: FLAP Antibody [NB300-891] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.](http://images.novusbio.com/fullsize/FLAP-Antibody-Immunohistochemistry-Paraffin-NB300-891-img0002.jpg)
![Flow Cytometry: FLAP Antibody [NB300-891] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/FLAP-Antibody-Flow-Cytometry-NB300-891-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: FLAP Antibody [NB300-891] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong endplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/FLAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-891-img0004.jpg)
![Immunohistochemistry-Paraffin: FLAP Antibody [NB300-891] - Staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. Antibody at 6 ug/mL.](http://images.novusbio.com/fullsize/FLAP-Antibody-Immunohistochemistry-Paraffin-NB300-891-img0003.jpg)
![Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (AA1) [NBP2-26444] - Human tonsil stained with Mast Cell Tryptase antibody.](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-AA1-Immunohistochemistry-Paraffin-NBP2-26444-img0005.jpg "Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (AA1) [NBP2-26444] - Human tonsil stained with Mast Cell Tryptase antibody.")
![Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (AA1) [NBP2-26444] - Analysis of Mast Cell Tryptase formalin-fixed paraffin embedded human tonsil using NBP2-26444.](http://images.novusbio.com/fullsize/Tryptase-alpha-TPS1-Antibody-AA1-Immunohistochemistry-Paraffin-NBP2-26444-img0003.jpg)
![Immunohistochemistry-Paraffin: Mouse Monoclonal Tryptase alpha/beta 1 Antibody (AA1) [IMGENEX: IMG-80250] [NBP2-26444] - Analysis of Tryptase alpha/beta 1 in human liver tissue. Mast cell positive for Tryptase alpha/beta 1. pAb: x500 and DAPI counter stain. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-26444_mouse-monoclonal-tryptase-alpha-beta-1-antibody-aa1-imgenex-img-80250-immunohistochemistry-paraffin-722025161826..jpg)
![Western Blot: RelA/NFkB p65 [p Ser529, p Ser536] Inhibitor Peptide Set [NBP2-29321] - Inhibits TNF-induced NF-kB activation: KBM-5 cells were incubated with various concentrations of peptides for 1 hr and treated with 0.1nM TNF for 30min. Nuclear extracts were prepared and NF-kB activation was analyzed by EMSA (Please refer toTakada et al, 2004 for further details). PTD-p65-P6 suppresses TNF-induced NF-kB activation by 25% at 100 uM and completely at 150 uM, where as the control peptide, PTD or p65-P6 without PTD did not have any effect.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529--p-Ser536]-Inhibitor-Peptide-Set-Western-Blot-NBP2-29321-img0015.jpg "Western Blot: RelA/NFkB p65 [p Ser529, p Ser536] Inhibitor Peptide Set [NBP2-29321] - Inhibits TNF-induced NF-kB activation: KBM-5 cells were incubated with various concentrations of peptides for 1 hr and treated with 0.1nM TNF for 30min. Nuclear extracts were prepared and NF-kB activation was analyzed by EMSA (Please refer toTakada et al, 2004 for further details). PTD-p65-P6 suppresses TNF-induced NF-kB activation by 25% at 100 uM and completely at 150 uM, where as the control peptide, PTD or p65-P6 without PTD did not have any effect.")
![Western Blot: RelA/NFkB p65 [p Ser529, p Ser536] Inhibitor Peptide Set [NBP2-29321] - Effect of various p65-inhibitor peptides on binding of purified recombinant p65 protein to the DNA in vitro. Recombinant p65 protein (100 ng/sample) was incubated for 30 minutes with 50uM of various peptides in 0.025 ml and then assayed for DNA binding activity by EMSA (Please refer to Takada et al, 2004 (3) for further details).](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529--p-Ser536]-Inhibitor-Peptide-Set-Western-Blot-NBP2-29321-img0013.jpg)
![Functional (Inhibition): RelA/NFkB p65 [p Ser529, p Ser536] Inhibitor Peptide Set [NBP2-29321] - TLR5/NF-kB/SEAPorter HEK 293 (NBP2-26277) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-29321) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Flagellin (NBP2-25289) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).](http://images.novusbio.com/fullsize/RelA-NFkB-p65-p-Ser529-p-Ser536-Inhibitor-Peptide-Set-Functional-Inhibition-NBP2-29321-img0012.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) [NBP2-32932] - Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD34 MAb (QBEnd/10)](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-Immunohistochemistry-Paraffin-NBP2-32932-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) [NBP2-32932] - Formalin-paraffin human tonsil (20X) stained with CD34 MAb (QBEnd/10)](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-Immunohistochemistry-Paraffin-NBP2-32932-img0002.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein. OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE and subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600-667 of isoform 1 of OS-9. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0019151), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/OS9-Antibody-Western-Blot-BC100-520-img0004.jpg "Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein. OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE and subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600-667 of isoform 1 of OS-9. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0019151), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: OS9 Antibody [BC100-520] - OS9 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/OS9-Antibody-Immunocytochemistry-Immunofluorescence-BC100-520-img0002.jpg)
![Western Blot: OS9 Antibody [BC100-520] - WB analysis of OS9 in 1. A431 cell lysate, 2. HepG2 cell lysate, 3. MCF7 cell lysate and 4. 3T3 cell lysate.](http://images.novusbio.com/fullsize/OS9-Antibody-Western-Blot-BC100-520-img0001.jpg)
![Simple Western: OS9 Antibody [BC100-520] - Simple Western lane view shows a specific band for OS9 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/OS9-Antibody-Simple-Western-BC100-520-img0003.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein.(A) OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE & subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600–667 of isoform 1 of OS-9. (B) Protein stability assay of endogenous OS-9. U2OS cells were treated with the translational inhibitor cycloheximide (100 µM). At indicated time points, whole cell lysates were analysed by immunoblotting. (C) Effect of hypoxia on OS-9 expression. For hypoxia, UT-7 cells were exposed to 1% O2 for 24 h prior to Western blot analysis. To determine any influence of HIF-1 alpha on OS-9 expression under normoxia, cells were incubated with the prolyl hydroxylase inhibitor DMOG (0.5 mM) for 24 h. (D) Protein interaction between OS-9 & PHD2 in vitro. For co-immunoprecipitation, U2OS cells were transiently co-transfected with the plasmids pOS-9-V5 & pPHD2-His, lysed in NP40 buffer, & subjected to immunoisolation with anti-V5 antibody recognizing OS-9 by its V5-tag. OS-9 & its associated proteins were separated by SDS-PAGE & analyzed by Western blot (lane 2). As controls, samples of untransfected (lane 1) cells or cells transfected with a single plasmid (lanes 3–4) were loaded. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415482216.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein.(A) OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE & subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600–667 of isoform 1 of OS-9. (B) Protein stability assay of endogenous OS-9. U2OS cells were treated with the translational inhibitor cycloheximide (100 µM). At indicated time points, whole cell lysates were analysed by immunoblotting. (C) Effect of hypoxia on OS-9 expression. For hypoxia, UT-7 cells were exposed to 1% O2 for 24 h prior to Western blot analysis. To determine any influence of HIF-1 alpha on OS-9 expression under normoxia, cells were incubated with the prolyl hydroxylase inhibitor DMOG (0.5 mM) for 24 h. (D) Protein interaction between OS-9 & PHD2 in vitro. For co-immunoprecipitation, U2OS cells were transiently co-transfected with the plasmids pOS-9-V5 & pPHD2-His, lysed in NP40 buffer, & subjected to immunoisolation with anti-V5 antibody recognizing OS-9 by its V5-tag. OS-9 & its associated proteins were separated by SDS-PAGE & analyzed by Western blot (lane 2). As controls, samples of untransfected (lane 1) cells or cells transfected with a single plasmid (lanes 3–4) were loaded. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415483621.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Initial characterization of the OS-9 protein.(A) OS-9 expression in various human cell lines. Equal protein amounts of total cell lysates were used for SDS-PAGE & subsequent Western blotting. For each cell line, two independent samples are shown. Endogenous OS-9 was detected with a polyclonal antibody raised against a peptide corresponding to amino acids 600–667 of isoform 1 of OS-9. (B) Protein stability assay of endogenous OS-9. U2OS cells were treated with the translational inhibitor cycloheximide (100 µM). At indicated time points, whole cell lysates were analysed by immunoblotting. (C) Effect of hypoxia on OS-9 expression. For hypoxia, UT-7 cells were exposed to 1% O2 for 24 h prior to Western blot analysis. To determine any influence of HIF-1 alpha on OS-9 expression under normoxia, cells were incubated with the prolyl hydroxylase inhibitor DMOG (0.5 mM) for 24 h. (D) Protein interaction between OS-9 & PHD2 in vitro. For co-immunoprecipitation, U2OS cells were transiently co-transfected with the plasmids pOS-9-V5 & pPHD2-His, lysed in NP40 buffer, & subjected to immunoisolation with anti-V5 antibody recognizing OS-9 by its V5-tag. OS-9 & its associated proteins were separated by SDS-PAGE & analyzed by Western blot (lane 2). As controls, samples of untransfected (lane 1) cells or cells transfected with a single plasmid (lanes 3–4) were loaded. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415482293.jpg)
![Western Blot: OS9 Antibody [BC100-520] - Cellular localization of OS-9 & PHD2.(A) A lectin gel-shift assay was conducted to test for glycosylated proteins. Total cell lysates of U2OS cells were incubated in the presence or absence of the endoglycosidases EndoH & PNGaseF for 6 h at 37°C. Digest products were separated on a reducing 10% SDS-PAGE gel which contained concanavalin A co-polymerized in the top layer of the separating gel to retard mobility of glycosylated proteins [56]. Glycosylated OS-9 is indicated as ‘g’, deglycosylated OS-9 as ‘d’. (B) Detection of OS-9 & PHD2 in the nuclear fraction. HEK293 cells with & without transfection of the plasmid pcDNA3-OS-9 were separated into nuclear fraction (N) & postnuclear supernatant (PS), the latter containing cytoplasm & organelles. Western blot analysis included BiP, GAPDH & lamin A as typical marker proteins for the ER, the cytoplasm & the nucleus, respectively. (C) Detection of OS-9 & PHD2 in the cytoplasm. HEK293 cells were co-transfected with pcDNA3-OS-9 & pPHD2-V5. For hypoxia, cells were exposed to 3% O2 for 4 h. Cells were treated with 50 µg/ml digitonin & centrifuged to obtain a cytoplasmic (C) & an organelle fraction (O) & subjected to immunoblotting. (D) Isolation of cellular endomembranes. HEK293 cells were lysed mechanically by several passages through a 30½G needle. The postnuclear supernatant was processed further by ultra-centrifugation to separate the organelles (O) from the cytosol (C). High salt treatment (1 M KCl) of the organelle fraction produced a wash fraction (W) that contained dissociated peripheral membrane proteins. For immunoblot analysis of subcellular fractionations, cell aliquots were normalized for cell number prior to loading (B–D). Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415483637.jpg)
![Western Blot: OS9 Antibody [BC100-520] - OS-9 shows no effect on regulation of HIF-1 alpha .Total cell lysates were used for SDS-PAGE & subsequent Western blotting. To generate nearly anoxic conditions, cells were exposed to an oxygen consuming chemical system or to 1% or 3% O2 for 4 h to generate hypoxia. (A) U2OS, HeLa & Hep3B cells were transiently transfected with the plasmid pOS-9-V5 48 h prior to the experiment. Lamin A & actin were used as loading controls. (B) U2OS cells were subjected to ER stress by incubation either with tunicamycin (1 µg/ml) or thapsigargin (0.5 µg/ml) for 20 h. To detect HIF-1 alpha under normoxia, cells were treated with DMOG (1 mM) for 4 h. A sample of DMSO-only treated cells was loaded to exclude unspecific side effects of the solvent. (C) U2OS cells were transduced with lentiviral construct pLKO.1-shRNA-OS-9 (shOS-9) mediating a stable knockdown of OS-9 expression. Control cells (c) were transduced with plasmid pLKO.1-puro. Representative Western blots are shown for each subfigure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21559462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-520_rabbit-polyclonal-os9-antibody-310202415482250.jpg)
![Western Blot: Bim Antibody [NBP1-76963] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), BIM NBP1-77118, (5 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0012.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Bim Antibody [NBP1-76963] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), BIM NBP1-77118, (5 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Independent Antibodies Validation. </b>Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Rat Myeloma Cell line:Loading: 15 ug of rat myeloma YB2/0 cell lysate per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0008.jpg)
![Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in HeLa Cells. Loading: 15 ug of lysate per lane. Antibodies: Bim NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate. Bim antibody can detect all three human isoforms, including EL, L and S isoforms.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0009.jpg)
![Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Human Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0010.jpg)
![Western Blot: Bim Antibody - BSA Free [NBP1-76963] - Figure 3 Western Blot Validation in Human TissueLoading: 15 u of lysates per lane.Antibodies: BIM , (0.5 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human thymusLane 2: Human colon](http://images.novusbio.com/fullsize/nbp1-76963_rabbit-polyclonal-bim-antibody-2711202420115554.gif)
![Immunocytochemistry/ Immunofluorescence: Bim Antibody - BSA Free [NBP1-76963] - Validation of BIM in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Bim with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76963_rabbit-polyclonal-bim-antibody-271120242045646.gif)
![Western Blot: TLR3 [p Tyr759] Antibody [NBP2-24904] - Image from verified customer review.](http://images.novusbio.com/fullsize/TLR3-[p-Tyr759]-Antibody-Western-Blot-NBP2-24904-img0003.jpg "Western Blot: TLR3 [p Tyr759] Antibody [NBP2-24904] - Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: TLR3 [p Tyr759] Antibody [NBP2-24904] - Analysis of IgG, AID and TLR3 (A) and a hyper-IgM (HIGM) syndrome patient (B and C). A and B: Paraformaldehyde-fixed, frozen tonsillar mucosa tissue sections. C. Paraformaldehyde-fixed, mucosal B cells from the colon lamina propria of the HIGM patient. Arrowheads indicate cells co-expressing phospho TLR3, AID, and IgG. Data courtesy of W. Xu. et. al., and the Journal of Immunology](http://images.novusbio.com/fullsize/TLR3-[p-Tyr759]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24904-img0002.jpg)
![Western Blot: BP1 Antibody [NB100-481] - Detection of MDA-MB-231 cell lysates.](http://images.novusbio.com/fullsize/BP1-Antibody-Western-Blot-NB100-481-img0004.jpg "Western Blot: BP1 Antibody [NB100-481] - Detection of MDA-MB-231 cell lysates.")
![Immunohistochemistry-Frozen: BP1 Antibody [NB100-481] - Top Panel: BP1 antibody detected in breast cancer cells. Bottom Panel: Staining of normal breast tissue.](http://images.novusbio.com/fullsize/BP1-Antibody-Immunohistochemistry-Frozen-NB100-481-img0005.jpg)
![Western Blot: BP1 Antibody [NB100-481] - Effect of BP1 on TNFalpha-induced cell death. Western blot analysis of Beta Protein 1 (BP1) protein expression in MCF7/EV cell lines and MCF7/BP1 cell lines. Image collected and cropped by CiteAb from the following publication (//breast-cancer-research.biomedcentral.com/articles/10.1186/bcr1766), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BP1-Antibody-Western-Blot-NB100-481-img0007.jpg)
![Immunoprecipitation: BP1 Antibody [NB100-481] - Antibody diluted 1:5,000 2. BP1-specific band after immunoprecipitation of 850 ug of cell extract using anti-BP1 (NB100-481) antibody diluted 1:1,000 3. Negative control for IP in lane 2: cell extract incubated with 50ul of Protein A/G beads.](http://images.novusbio.com/fullsize/BP1-Antibody-Immunoprecipitation-NB100-481-img0006.jpg)
![Western Blot: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - Aanalysis in HeLa whole cell extracts using EGLN1/PHD2 antibody NBP1-30328.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-366G-76-3-Western-Blot-NBP1-30328-img0004.jpg "Western Blot: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - Aanalysis in HeLa whole cell extracts using EGLN1/PHD2 antibody NBP1-30328.")
![Immunohistochemistry: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - Analysis of PHD2 in human renal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-366G-76-3-Immunohistochemistry-NBP1-30328-img0005.jpg)
![Simple Western: EGLN1/PHD2 Antibody (366G/76/3) [NBP1-30328] - Lane view shows a specific band for EGLN1/PHD2 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-366G-76-3-Simple-Western-NBP1-30328-img0006.jpg)
![Western Blot: CD44 Antibody (Hermes-1) [NBP2-22530] - of Hela cell lysates.](http://images.novusbio.com/fullsize/CD44-Antibody-Hermes-1-Western-Blot-NBP2-22530-img0002.jpg "Western Blot: CD44 Antibody (Hermes-1) [NBP2-22530] - of Hela cell lysates.")
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (Hermes-1) [NBP2-22530] - Analysis of CD44 (green) showing staining in the membrane of Hela cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CD44-Antibody-Hermes-1-Immunocytochemistry-Immunofluorescence-NBP2-22530-img0004.jpg)
![Flow Cytometry: CD44 Antibody (Hermes-1) [NBP2-22530] - A surface stain was performed on U-87 cells with CD44 (Hermes-1) Antibody NBP2-22530R (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/fullsize/CD44-Antibody-Hermes-1-Flow-Cytometry-NBP2-22530-img0006.jpg)
![Flow Cytometry: CD44 Antibody (Hermes-1) [NBP2-22530] - Analysis of CD44 in PBMC cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/CD44-Antibody-Hermes-1-Flow-Cytometry-NBP2-22530-img0003.jpg)
![Flow Cytometry: CD44 Antibody (Hermes-1) [NBP2-22530] - A surface stain was performed on U-87 cells with CD44 [Hermes-1] Antibody NBP2-22530AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/CD44-Antibody-Hermes-1-Flow-Cytometry-NBP2-22530-img0005.jpg)
![Immunohistochemistry-Paraffin: TNF-alpha Antibody (52B83) [NB600-1422] - TNF alpha in sheep tonsil FFPE. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-52B83-Immunohistochemistry-Paraffin-NB600-1422-img0001.jpg "Immunohistochemistry-Paraffin: TNF-alpha Antibody (52B83) [NB600-1422] - TNF alpha in sheep tonsil FFPE. IHC-P image submitted by a verified customer review.")
![Western Blot: OS9 Antibody [NB100-519] - Contribution and interaction of OS-9 for degradation of SHH proteins. Cells stably expressing the N278A-HA precursor were depleted of OS-9 by siRNA, and the fate of N278A was followed after cycloheximide (CHX) addition. All samples were analyzed by SDS-PAGE and immunoblotting with HA antibodies. Controls were treated with a commercial negative control siRNA. The samples were also immunoblotted for OS-9 to show the extent of depletion. Immunoblotting with GAPDH antibodies served as loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092164) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/OS9-Antibody-Knockdown-Validated-NB100-519-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: OS9 Antibody [NB100-519] - Contribution and interaction of OS-9 for degradation of SHH proteins. Cells stably expressing the N278A-HA precursor were depleted of OS-9 by siRNA, and the fate of N278A was followed after cycloheximide (CHX) addition. All samples were analyzed by SDS-PAGE and immunoblotting with HA antibodies. Controls were treated with a commercial negative control siRNA. The samples were also immunoblotted for OS-9 to show the extent of depletion. Immunoblotting with GAPDH antibodies served as loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092164) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: OS9 Antibody [NB100-519] - Detection of OS-9.1 and OS-9.2 in total HEK293 cells, HEK293 cells expressing OS-9.1 or OS-9.2 by western analysis.](http://images.novusbio.com/fullsize/OS9-Antibody-Western-Blot-NB100-519-img0005.jpg)
![Immunohistochemistry: OS9 Antibody [NB100-519] - Staining of OS9 in mouse smooth muscle.](http://images.novusbio.com/fullsize/OS9-Antibody-Immunohistochemistry-NB100-519-img0009.jpg)
![Immunoprecipitation: OS9 Antibody [NB100-519] - Immunoprecipitation of OS-9.1 and OS-9.2 in total HEK293 cells, HEK293 cells expressing OS-9.1 or OS-9.2.](http://images.novusbio.com/fullsize/OS9-Antibody-Immunoprecipitation-NB100-519-img0008.jpg)