| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43262-0.5mg | |||
| BSA Free | NBP1-43262-25ug | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0131A488-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25255SS | |||
| Azide and BSA Free | NBP2-80588 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64988SS | |||
| Azide and BSA Free | NBP2-80647 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56582SS | |||
| Azide and BSA Free | NBP2-33297 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56542SS | |||
| Azide and BSA Free | NBP2-33224 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54392-100ug | |||
![Western Blot: TLR3 [p Tyr759] Antibody [NBP2-24904] - Image from verified customer review.](http://images.novusbio.com/fullsize/TLR3-[p-Tyr759]-Antibody-Western-Blot-NBP2-24904-img0003.jpg "Western Blot: TLR3 [p Tyr759] Antibody [NBP2-24904] - Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: TLR3 [p Tyr759] Antibody [NBP2-24904] - Analysis of IgG, AID and TLR3 (A) and a hyper-IgM (HIGM) syndrome patient (B and C). A and B: Paraformaldehyde-fixed, frozen tonsillar mucosa tissue sections. C. Paraformaldehyde-fixed, mucosal B cells from the colon lamina propria of the HIGM patient. Arrowheads indicate cells co-expressing phospho TLR3, AID, and IgG. Data courtesy of W. Xu. et. al., and the Journal of Immunology](http://images.novusbio.com/fullsize/TLR3-[p-Tyr759]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24904-img0002.jpg)
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Using the Allophycocyanin direct conjugate A cell surface stain was performed on RAW246.7 cells with B7-H1/PD-L1/CD274 (MIH5) antibody NBP1-43262APC (blue) and a matched isotype control NBP1-51104APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0020.jpg "Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Using the Allophycocyanin direct conjugate A cell surface stain was performed on RAW246.7 cells with B7-H1/PD-L1/CD274 (MIH5) antibody NBP1-43262APC (blue) and a matched isotype control NBP1-51104APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.")
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Analysis using the Biotin conjugate of NBP1-43262. Staining of C57Bl/6 splenocytes with 0.125 ug of Rat IgG2a Isotype Control Biotin (open histogram) or 0.125 ug of Anti-Mouse (B7-H1) Biotin (filled histogram) followed by Streptavidin PE.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0019.jpg)
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - B7-H1/PD-L1/CD274 Antibody (MIH5) [NBP1-43262] - Staining of mouse splenocytes with Anti-Mouse B7-H1/PD-L1/CD274) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0021.jpg)
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - Analysis using the DyLight 488 conjugate of NBP1-43262. Staining of PD-L1 in human primary colon cancer cell using anti-PD-L1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0011.jpg)
![Flow Cytometry: PD-L1/B7-H1 Antibody (MIH5) [NBP1-43262] - Analysis using the DyLight 488 conjugate of NBP1-43262. Staining of PD-L1 in HT29 cells using anti-PD-L1 antibody. The data shows the detection of total PD-L1 status in the cancer cells. Image from verified customer review.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-MIH5-Flow-Cytometry-NBP1-43262-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (mAb89) [DDX0131P-100] - CD40-transfected L cells stained.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-mAb89-Immunocytochemistry-Immunofluorescence-DDX0131P-100-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (mAb89) [DDX0131P-100] - CD40-transfected L cells stained.")
![Immunohistochemistry: CD40/TNFRSF5 Antibody (mAb89) [DDX0131P-100] - IHC staining on Bouin paraffinlymph node section. HumanCD40 stained with mAb 89.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-mAb89-Immunohistochemistry-DDX0131P-100-img0004.jpg)
![Western Blot: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Analysis in human Raji lymphoma cells.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-62N3G8-Western-Blot-NBP2-25255-img0010.jpg)
![Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Human thymus stained with CD80 antibody at 5 ug/ml using peroxidase-conjugate and DAB chromogen. Note membrane and cytoplasmic staining.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-62N3G8-Immunohistochemistry-Paraffin-NBP2-25255-img0002.jpg)
![Flow Cytometry: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Raji lymphoma cells. Image from verified customer review.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-62N3G8-Flow-Cytometry-NBP2-25255-img0009.jpg)
![Western Blot: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Analysis of CD80 in A) a partial recombinant protein and B) Daudi lysate using this antibody at 0.1 and 1 g/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-62N3G8-Western-Blot-NBP2-25255-img0003.jpg)
![Flow Cytometry: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-25255. Staining of CD80 in human PBMC (monocyte depleted) using anti-CD80 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-62N3G8-Flow-Cytometry-NBP2-25255-img0008.jpg)
![Flow Cytometry: CD4 Antibody (W3/25) [NB100-64988] - Detection of CD4 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with (A) Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # NB100-64988) or (B) Mouse IgG2A Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Rat CD3 epsilon PE conjugated Monoclonal Antibody.](http://images.novusbio.com/fullsize2/CD4-Antibody-W3-25-Flow-Cytometry-NB100-64988-img0008.jpg "Flow Cytometry: CD4 Antibody (W3/25) [NB100-64988] - Detection of CD4 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with (A) Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # NB100-64988) or (B) Mouse IgG2A Isotype Control Antibody (Catalog # MAB003) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B) and Mouse Anti-Rat CD3 epsilon PE conjugated Monoclonal Antibody.")
![Flow (Cell Surface): CD4 Antibody (W3/25) [NB100-64988] - Rat Splenocytes were stained with NB100-64988 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alex Fluor 488.](http://images.novusbio.com/fullsize2/CD4-Antibody-W3-25-Flow-Cell-Surface-NB100-64988-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD4 Antibody (W3/25) [NB100-64988] - Staining of rat splenocytes with CD4 (W3/25) antibody. Live cells were incubated with CD4 antibody on ice, then fixed with 2% formaldehyde prior to secondary antibody incubation.](http://images.novusbio.com/fullsize2/CD4-Antibody-W3-25-Immunocytochemistry-Immunofluorescence-NB100-64988-img0007.jpg)
![Immunohistochemistry-Frozen: CD4 Antibody (W3/25) [NB100-64988] - Staining of frozen rat spleen with Mouse anti Rat CD4.](http://images.novusbio.com/fullsize/CD4-Antibody-W3-25-Immunohistochemistry-Frozen-NB100-64988-img0001.jpg)
![Flow Cytometry: CD4 Antibody (W3/25) [NB100-64988] - Staining of stimulated rat spleen cells.](http://images.novusbio.com/fullsize2/CD4-Antibody-W3-25-Flow-Cytometry-NB100-64988-img0003.jpg)
![Western Blot: OCIL/CLEC2d Antibody (4C7) [H00029121-M01] - Analysis of CLEC2D expression in transfected 293T cell line by CLEC2D monoclonal antibody (M01), clone 4C7.Lane 1: CLEC2D transfected lysate(21.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/OCIL-CLEC2d-Antibody-4C7-Western-Blot-H00029121-M01-img0005.jpg "Western Blot: OCIL/CLEC2d Antibody (4C7) [H00029121-M01] - Analysis of CLEC2D expression in transfected 293T cell line by CLEC2D monoclonal antibody (M01), clone 4C7.Lane 1: CLEC2D transfected lysate(21.8 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: OCIL/CLEC2d Antibody (4C7) [H00029121-M01] - Analysis of monoclonal antibody to CLEC2D on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/OCIL-CLEC2d-Antibody-4C7-Immunohistochemistry-Paraffin-H00029121-M01-img0003.jpg)
![ELISA: OCIL/CLEC2d Antibody (4C7) [H00029121-M01] - Detection limit for recombinant GST tagged CLEC2D is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/OCIL-CLEC2d-Antibody-4C7-ELISA-H00029121-M01-img0006.jpg)
![Western Blot: TLR2 Antibody - Azide Free [NBP2-24861] - Analysis of TLR2 in transfected cell lysate using this antibody.](http://images.novusbio.com/fullsize/TLR2-Antibody---Azide-Free-Western-Blot-NBP2-24861-img0001.jpg "Western Blot: TLR2 Antibody - Azide Free [NBP2-24861] - Analysis of TLR2 in transfected cell lysate using this antibody.")
![Immunohistochemistry: TLR2 Antibody - Azide Free [NBP2-24861] - Analysis of mouse pancreas tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml. Image using the standard format of this product.](http://images.novusbio.com/fullsize/TLR2-Antibody---Azide-Free-Immunohistochemistry-NBP2-24861-img0003.jpg)
![Immunohistochemistry: TLR2 Antibody - Azide Free [NBP2-24861] - Representative photographs of immunohistochemistry for tubular TLR2 in G) ctrl, H) ctrl + empa, I) diabetic (dm), J) dm + empagliflozin, K) dm + telmisartan groups (Magnification = original X 200) and quantification of tubular TLR2 expression by Image J (L). (Data are expressed as mean +/- SEM with *=P<0.05 vs ctrl, **=P<0.001 vs ctrl and #=P<0.05 vs dm). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0108994) licensed under a CC-BY license. Image using the standard format of this product.](http://images.novusbio.com/fullsize/TLR2-Antibody---Azide-Free-Immunohistochemistry-NBP2-24861-img0002.jpg)
![Immunohistochemistry: HLA G Antibody (MEM-G/1) [NB500-302] - ELA affects first trimester placental explant trophoblast proliferation. HLA-G immunostaining (brown color) indicates the location of extravillous trophoblasts. In control explants (left panel) almost all villous cytotrophoblasts proliferate, while this is much less apparent in explants treated with ELA (0.25nM, right panel). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-55650-5) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-1-Immunohistochemistry-NB500-302-img0005.jpg "Immunohistochemistry: HLA G Antibody (MEM-G/1) [NB500-302] - ELA affects first trimester placental explant trophoblast proliferation. HLA-G immunostaining (brown color) indicates the location of extravillous trophoblasts. In control explants (left panel) almost all villous cytotrophoblasts proliferate, while this is much less apparent in explants treated with ELA (0.25nM, right panel). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-55650-5) licensed under a CC-BY license.")
![Western Blot: HLA G Antibody (MEM-G/1) [NB500-302] - Using the Biotin direct conjugate analysis (reducing conditions) of HLA-G1 in HLA-G1 transfectants using the antibody MEM-G/1 biotin.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-1-Western-Blot-NB500-302-img0003.jpg)
![Immunohistochemistry: HLA G Antibody (MEM-G/1) [NB500-302] - Fig. 1B - first-trimester placenta (paraffin-embedded sections)](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-1-Immunohistochemistry-NB500-302-img0001.jpg)
![Immunohistochemistry: HLA G Antibody (MEM-G/1) [NB500-302] - Fig. 1. Immunohistochemistry staining with anti-human HLA-G (MEM-G/1). Fig. 1A - pulmonary disseases (paraffin-embedded sections) The antibody MEM-G/1 stains infiltrating macrophages in pulmonary diseases. In the top left corner see the detail of macrophage.](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-1-Immunohistochemistry-NB500-302-img0002.jpg)
![Immunohistochemistry: HLA G Antibody (MEM-G/1) - BSA Free [NB500-302] - ELA affects first trimester placental explant trophoblast proliferation. (a) HLA-G immunostaining (brown color top panel) indicates the location of extravillous trophoblasts. PhosphoH3(Ser10) immunostaining (brown color lower 3 panels) identifies proliferating cells undergoing mitosis. In extravillous trophoblasts of explants treated with ELA proliferation appears to decrease. In control explants almost all villous cytotrophoblasts proliferate, while this is much less apparent in explants treated with ELA. Treatment with ELA in combination with ML221 does not return villous cytotrophoblast proliferation to the level as seen in control explants. (b) Quantifying the percentage of proliferating extravillous trophoblasts observed by immunohistochemistry shows a significant decrease upon treatment with ELA which cannot be rescued by the addition of ML221. P = 0.08 between controls & explants treated with ELA & ML221. n = 12 placentas, 5 explants per treatment. Bars represent mean ± SEM & data was tested with one-way ANOVA followed by Bonferroni multiple comparisons test. *Indicates p < 0.05. (c) Treatment of explants with an ELA neutralizing antibody shows a significant increase of extravillous trophoblast proliferation upon quantifying immunohistochemistry stainings. n = 4 placentas, 5 explants per treatment. Bars represent mean ± SEM & data was tested with a Student’s t-test. *Indicates p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31836787), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-302_mouse-monoclonal-hla-g-antibody-mem-g-1-31020241617149.jpg)
![Western Blot: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Detection of 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/mL.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Western-Blot-NB100-56582-img0018.jpg "Western Blot: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Detection of 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/mL.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the Allophycocyanin conjugate of NB100-56582. Staining of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and this antibody. 1 ug/10^6 cells. Products used: anti-CD4 FITC conjugate.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0010.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the FITC conjugate of NB100-56582. Staining of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and this antibody, 5 ug/10^6 cells. Products used: anti-CD4 PE conjugate, anti-CD25 APC conjugate, anti-FOXP3 FITC conjugate.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0013.jpg)
![Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Staining of 2 x 10^6 mouse lymph node cells using 0.1 ug of PE conjugated antibody.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0012.jpg)
![Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Staining of human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and NBP2-33297PE (right) at 0.1 ug/10^6 cells. Products used: anti-CD4 FITC conjugate, anti-CD25 APC conjugate.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0017.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the Allophycocyanin conjugate of NB100-56582. Staining of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control (left) and (right) at 0.1 ug/10^6 cells. Anti-CD25 FITC conjugate this antibody, anti-FOXP3 FITC.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0011.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the FITC conjugate of NB100-56582. Staining of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control (left) and (right) at 0.5 ug/10^6 cells. Anti-CD25 APC conjugate this antibody, anti-FOXP3 FITC.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0014.jpg)
![Western Blot: IKK gamma Antibody (72C627) [NB100-56542] - Analysis using IKK gamma (NEMO) antibody. Lysate from 1) human Jurkat and 2) mouse NIH 3T3 cells probed with IKK gamma antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-72C627-Western-Blot-NB100-56542-img0005.jpg "Western Blot: IKK gamma Antibody (72C627) [NB100-56542] - Analysis using IKK gamma (NEMO) antibody. Lysate from 1) human Jurkat and 2) mouse NIH 3T3 cells probed with IKK gamma antibody at 2 ug/ml.")
![Simple Western: IKK gamma Antibody (72C627) [NB100-56542] - Simple Western lane view shows a specific band for IKK gamma in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-72C627-Simple-Western-NB100-56542-img0004.jpg)
![Western Blot: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (C3e/1308).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1308-Western-Blot-NBP2-53387-img0005.jpg)
![Flow Cytometry: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Flow Cytometric Analysis of Jurkat cells using CD3 epsilon Antibody (C3e/1308) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1308-Flow-Cytometry-NBP2-53387-img0004.jpg)
![Western Blot: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Western Blot of recombinant CD3e and Jurkat Cell Lysate using CD3e Monoclonal Antibody (C3e/1308)](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1308-Western-Blot-NBP2-53387-img0002.jpg)
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Staining of T cells in human FFPE tonsil tissue using AF647-conjugated CD3 epsilon Antibody (Catalog # NBP2-54392AF647). Antigen Retrieval at pH 9. Antibody incubation over night at 4C. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-53387_mouse-monoclonal-cd3-epsilon-antibody-c3e-1308-17720241003230.jpg)
![Immunohistochemistry-Paraffin: PD-L1/B7-H1 Antibody [NBP2-15791] - PD-L1 antibody detects PD-L1 protein at cell membrane in human ovarian carcinoma by immunohistochemical analysis. Antibodies: PD-L1 antibody, and competitor's antibody.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Immunohistochemistry-Paraffin-NBP2-15791-img0019.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PD-L1/B7-H1 Antibody [NBP2-15791] - PD-L1 antibody detects PD-L1 protein at cell membrane in human ovarian carcinoma by immunohistochemical analysis. Antibodies: PD-L1 antibody, and competitor's antibody.")
![<b>Independent Antibodies Validation. </b>Western Blot: PD-L1/B7-H1 Antibody [NBP2-15791] - Various whole cell extracts were separated by 10% SDS-PAGE, and the membranes were blotted with PD-L1 antibody diluted at 1:600 and with DDDDK tag antibody (NBP2-43574) diluted at 1:3000 to detect DDDDK-tagged PD-L2. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Western-Blot-NBP2-15791-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PD-L1 Antibody [NBP2-15791] - PD-L1/B7-H1 Antibody [NBP2-15791] - Non-transfected (-) and transfected (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Western-Blot-NBP2-15791-img0009.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PD-L1/B7-H1 Antibody [NBP2-15791] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Western-Blot-NBP2-15791-img0007.jpg)
![Western Blot: PD-L1 Antibody [NBP2-15791] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Western-Blot-NBP2-15791-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: PD-L1 Antibody [NBP2-15791] - MDA-MB-231 (left) and HeLa (right) cells were fixed in ice-cold MeOH for 5 min.Green: PD-L1 stained by PD-L1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15791-img0022.jpg)
![Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP2-15791] - Human ovarian cancer. PD-L1 stained by PD-L1 antibody diluted at 1:4000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PD-L1-Antibody-Immunohistochemistry-Paraffin-NBP2-15791-img0025.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: PD-L1 Antibody [NBP2-15791] - PD-L1/B7-H1 Antibody [NBP2-15791] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with PD-L1 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. NBP2-15791 on the left and competitor's antibody on the right.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Western-Blot-NBP2-15791-img0021.jpg)
![Immunohistochemistry-Paraffin: PD-L1/B7-H1 Antibody [NBP2-15791] - PD-L1 proteinat cell membrane in human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. PD-L1 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Immunohistochemistry-Paraffin-NBP2-15791-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PD-L1 Antibody [NBP2-15791] - Detection of cell membranes in PD-L1 protein-expressing cell lines by immunohistochemical analysis. Antibodies: PD-L1 antibody, and competitor's antibody. Samples: Negative (-), low positive (+), intermediate positive (++) and strong positive (+++) cell line cores assessed using Quantitative Digital Pathology.](http://images.novusbio.com/fullsize/PD-L1-B7-H1-Antibody-Immunohistochemistry-Paraffin-NBP2-15791-img0020.jpg)
![Western Blot: PD-L1 Antibody [NBP2-15791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (NBP2-15791) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15791_rabbit-polyclonal-pd-l1-antibody-91020242075966.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PD-L1 Antibody [NBP2-15791] - Wild-type (WT) and PD-L1 knockout (KO) MDA-MB-231 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15791_rabbit-polyclonal-pd-l1-antibody-1610202419405783.jpg)
