| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0490A488 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-15180SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71006 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32500-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27214 | |||
| BSA Free | NBP1-70343SS | |||
![TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - TLR2 acts through formation of heterodimer complexes with TLR1 or TLR6. HEK 293 endogenously expresses TLR1 and TLR6, so that the TLR2 reporter cell line can response to both Pam3CSK4 (TLR2/TLR1 specific ligand) and MALP-2 (TLR2/TLR6 specific ligand). The TLR2 / TLR4 inhibitor specifically inhibits TLR2/TLR1 receptor complex activity in a dose-response manner but exhibited no or little effect on TLR2/TLR6 receptor complex activity.](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Inhibitor-Peptide-NBP2-26245-img0007.jpg "TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - TLR2 acts through formation of heterodimer complexes with TLR1 or TLR6. HEK 293 endogenously expresses TLR1 and TLR6, so that the TLR2 reporter cell line can response to both Pam3CSK4 (TLR2/TLR1 specific ligand) and MALP-2 (TLR2/TLR6 specific ligand). The TLR2 / TLR4 inhibitor specifically inhibits TLR2/TLR1 receptor complex activity in a dose-response manner but exhibited no or little effect on TLR2/TLR6 receptor complex activity.")
![TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - The TLR4 reporter cell line responds to LPS. The TLR2 / TLR4 inhibitor specifically inhibits TLR4 activation upon LPS stimulation in a dose-response manner.](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Inhibitor-Peptide-NBP2-26245-img0008.jpg)
![TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - TLR2 /4 Inhibitor suppressed the Pam3CSK4-induced TLR2/TLR1 activity [A] and the LPS-induced TLR4 activity [B] in a dose-response manner, of which IC50 values were measured as 17.2 uM and 10.47 uM, respectively.](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Inhibitor-Peptide-NBP2-26245-img0009.jpg)
![Western Blot: IFIT2 Antibody [NBP1-31164] - p115 and Sec16A are required for optimal IRF-3 activation in response to activation of cytosolic RNA and DNA sensors.HeLa cells were infected with lentiviral vectors encoding shRNA targeting p115. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1002747) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IFIT2-Antibody-Western-Blot-NBP1-31164-img0006.jpg "Western Blot: IFIT2 Antibody [NBP1-31164] - p115 and Sec16A are required for optimal IRF-3 activation in response to activation of cytosolic RNA and DNA sensors.HeLa cells were infected with lentiviral vectors encoding shRNA targeting p115. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1002747) licensed under a CC-BY license.")
![Western Blot: IFIT2 Antibody [NBP1-31164] - Sample(30 ug of whole cell lysate)A:A431 B:Hep G2 7.5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/IFIT2-Antibody-Western-Blot-NBP1-31164-img0003.jpg)
![Western Blot: IFIT2 Antibody [NBP1-31164] - p115 & Sec16A are required for optimal IRF-3 activation in response to activation of cytosolic RNA & DNA sensors.HeLa cells were infected with lentiviral vectors encoding shRNA targeting p115 (A) or Sec16A (B) & non-targeting (NT) control shRNA for 24 h followed by puromycin selection (1.5 µg/ml) for 5 days. Cells were left untreated or stimulated with poly I:C (1 µg/ml), poly dA:dT (1 µg/ml) or SeV (200 HAU/ml) for 16 h. Whole-cell lysates were prepared & subjected to immunoblot analysis with indicated antibodies. One of two independent experiments with similar results is shown. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22792062), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31164_rabbit-polyclonal-ifit2-antibody-310202415171321.jpg)
![IFIT2 antibody [N1], N-term detects IFIT2 protein at endoplasmic reticulum by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: IFIT2 protein stained by IFIT2 antibody [N1], N-term (NBP1-31164) diluted at 1:200.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-31164_rabbit-polyclonal-ifit2-antibody-immunocytochemistry-immunofluorescence-1411202518351210.jpg)
![Immunocytochemistry/Immunofluorescence: TLR10 Antibody (25F9) [DDX0490P-100] - TLR10 transfected COP cells](http://images.novusbio.com/fullsize/TLR10-Antibody-25F9-Immunofluorescence-DDX0490P-100-img0009.jpg "Immunocytochemistry/Immunofluorescence: TLR10 Antibody (25F9) [DDX0490P-100] - TLR10 transfected COP cells")
![Flow (Intracellular): TLR10 Antibody (25F9) [DDX0490P-100] - TLR10 transfected COP cells](http://images.novusbio.com/fullsize/TLR10-Antibody-25F9-Flow-Intracellular-DDX0490P-100-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: TLR10 Antibody (25F9) [DDX0490P-100] - Analysis using the Alexa Fluor (R) 488 conjugate of DDX0490P-100. Staining of hTLR10- transfected COP5 cells with 25F9.](http://images.novusbio.com/fullsize/TLR10-Antibody-25F9-Immunocytochemistry-Immunofluorescence-DDX0490P-100-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TLR10 Antibody (25F9) [DDX0490P-100] - Untransfected COP cells](http://images.novusbio.com/fullsize/TLR10-Antibody-25F9-Immunofluorescence-DDX0490P-100-img0008.jpg)
![Flow Cytometry: TLR10 Antibody (25F9) [DDX0490P-100] - Analysis using the Alexa Fluor (R) 488 conjugate of DDX0490P-100. Staining of TLR10-transfected COP5 cells with 25F9.](http://images.novusbio.com/fullsize/TLR10-Antibody-25F9-Flow-Cytometry-DDX0490P-100-img0004.jpg)
![Flow (Intracellular): TLR10 Antibody (25F9) [DDX0490P-100] - Untransfected COP cells](http://images.novusbio.com/fullsize/TLR10-Antibody-25F9-Flow-Intracellular-DDX0490P-100-img0006.jpg)
![Western Blot: TRAM/TICAM2 Antibody [NBP2-24686] - Analysis of TICAM2 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-24686 at 3 ug/ml.](http://images.novusbio.com/fullsize/TRAM-TICAM2-Antibody-Western-Blot-NBP2-24686-img0005.jpg "Western Blot: TRAM/TICAM2 Antibody [NBP2-24686] - Analysis of TICAM2 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-24686 at 3 ug/ml.")
![Immunohistochemistry-Paraffin: TRAM/TICAM2 Antibody [NBP2-24686] - Analysis of TAG/TIRAP3b in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/TRAM-TICAM2-Antibody-Immunohistochemistry-Paraffin-NBP2-24686-img0004.jpg)
![Western Blot: IFIT2 Antibody [NBP2-15180] - MAPL is required for early steps of immune response to Sendai virus infection. Maplfl/fl and Mapl-/- MEFs were left untreated or infected with Sendai virus (150 HAU/mL) for up to 18 hours and lysates were immunoblotted. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-00151-6), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IFIT2-Antibody-Western-Blot-NBP2-15180-img0004.jpg "Western Blot: IFIT2 Antibody [NBP2-15180] - MAPL is required for early steps of immune response to Sendai virus infection. Maplfl/fl and Mapl-/- MEFs were left untreated or infected with Sendai virus (150 HAU/mL) for up to 18 hours and lysates were immunoblotted. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-00151-6), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: IFIT2 Antibody [NBP2-15180] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-IFIT2 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/IFIT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15180-img0003.jpg)
![Western Blot: IFIT2 Antibody [NBP2-15180] - Analysis of IFIT2 in human K562 lysate in the 1) absence and 2) presence of immunizing peptide, and 3) mouse RAW lysate probed with 4 ug/ml of IFIT2 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IFIT2-Antibody-Western-Blot-NBP2-15180-img0002.jpg)
![Western Blot: TRAM/TICAM2 Antibody [NBP2-24638] - Lysate from human lung in the 1) absence, 2) presence of immunizing peptide, 3) mouse lung and 4) rat lung probed with TRAM antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TRAM-TICAM2-Antibody-Western-Blot-NBP2-24638-img0004.jpg "Western Blot: TRAM/TICAM2 Antibody [NBP2-24638] - Lysate from human lung in the 1) absence, 2) presence of immunizing peptide, 3) mouse lung and 4) rat lung probed with TRAM antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: TRAM/TICAM2 Antibody [NBP2-24638] - Staining of human brain tissue probed with an isotype control antibody (top left) and TRAM antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/TRAM-TICAM2-Antibody-Immunohistochemistry-Paraffin-NBP2-24638-img0003.jpg)
![Functional (Inhibition): TIRAP (TLR2 and TLR4) Inhibitor Peptide Set [NBP2-29331] - TLR2/NF-kB/SEAPorter HEK 293 (NBP2-26274) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-29331) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Pam3CSK4 (NBP2-25297) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Inhibitor-Peptide-Set-Functional-Inhibition-NBP2-29331-img0001.jpg "Functional (Inhibition): TIRAP (TLR2 and TLR4) Inhibitor Peptide Set [NBP2-29331] - TLR2/NF-kB/SEAPorter HEK 293 (NBP2-26274) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-29331) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Pam3CSK4 (NBP2-25297) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).")
![Western Blot: IFIT3 Antibody (OTI1G1) [NBP2-02148] - Analysis of extracts (35ug) from 9 different cell lines by using anti-IFIT3 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)](http://images.novusbio.com/fullsize/IFIT3-Antibody-OTI1G1-Western-Blot-NBP2-02148-img0010.jpg "Western Blot: IFIT3 Antibody (OTI1G1) [NBP2-02148] - Analysis of extracts (35ug) from 9 different cell lines by using anti-IFIT3 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)")
![Immunocytochemistry/Immunofluorescence: IFIT3 Antibody (OTI1G1) [NBP2-02148] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IFIT3.](http://images.novusbio.com/fullsize/IFIT3-Antibody-1G1-Immunocytochemistry-Immunofluorescence-NBP2-02148-img0009.jpg)
![Flow Cytometry: IFIT3 Antibody (1G1) [NBP2-02148] - Analysis of Jurkat cells, using anti-IFIT3 antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/IFIT3-Antibody-1G1-Flow-Cytometry-NBP2-02148-img0001.jpg)
![Western Blot: IFIT3 Antibody (1G1) [NBP2-02148] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFIT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFIT3.](http://images.novusbio.com/fullsize/IFIT3-Antibody-1G1-Western-Blot-NBP2-02148-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: IFIT3 Antibody (1G1) [NBP2-02148] - Immunofluorescent staining of HT29 cells using anti-IFIT3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IFIT3-Antibody-1G1-Immunocytochemistry-Immunofluorescence-NBP2-02148-img0003.jpg)
![IFIT3 Antibody (OTI1G1)[NBP2-02148]Simple Western� analysis of endogenous protein GBE1 from SH-SY5Y lysates (0.1mg/mL) using GBE1 Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at a 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp2-02148_mouse-ifit3-mab-oti1g1-simple-western-131120251236354.jpg%253fstore=america&image-type=image)
![Western Blot: IFIT3 Antibody [NBP2-32500] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-IFIT3 antibody. Corresponding IFIT3 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.](http://images.novusbio.com/fullsize/IFIT3-Antibody-Western-Blot-NBP2-32500-img0022.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: IFIT3 Antibody [NBP2-32500] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-IFIT3 antibody. Corresponding IFIT3 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: IFIT3 Antibody [NBP2-32500] - Staining of human cell line A-431 shows localization to cytosol & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/IFIT3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32500-img0016.jpg)
![Immunohistochemistry-Paraffin: IFIT3 Antibody [NBP2-32500] - Staining of human skeletal muscle shows no positivity in myocytes.](http://images.novusbio.com/fullsize/IFIT3-Antibody-Immunohistochemistry-Paraffin-NBP2-32500-img0026.jpg)
![Immunohistochemistry-Paraffin: IFIT3 Antibody [NBP2-32500] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/IFIT3-Antibody-Immunohistochemistry-Paraffin-NBP2-32500-img0020.jpg)
![Immunohistochemistry-Paraffin: IFIT3 Antibody [NBP2-32500] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/IFIT3-Antibody-Immunohistochemistry-Paraffin-NBP2-32500-img0024.jpg)
![Immunohistochemistry-Paraffin: IFIT3 Antibody [NBP2-32500] - Staining of human rectum shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/IFIT3-Antibody-Immunohistochemistry-Paraffin-NBP2-32500-img0025.jpg)
![Flow Cytometry: TLR10 Antibody (3C10C5) [NBP1-70343] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-27214. Detection of TLR10 on human PBMC using 10 ul (1 ug) of TLR10 antibody and 1 ug of CD19 antibody. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/TLR10-Antibody-3C10C5-Flow-Cytometry-NBP1-70343-img0005.jpg "Flow Cytometry: TLR10 Antibody (3C10C5) [NBP1-70343] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-27214. Detection of TLR10 on human PBMC using 10 ul (1 ug) of TLR10 antibody and 1 ug of CD19 antibody. Image using the Azide Free format of this antibody.")
![Flow Cytometry: TLR10 Antibody (3C10C5) [NBP1-70343] - Analysis using the PE conjugate of NBP2-27214. Staining of Human PBMC were probed using 10 ul (1 ug) of TLR10 antibody and 1 ug of CD19 antibody.](http://images.novusbio.com/fullsize/TLR10-Antibody-3C10C5-Flow-Cytometry-NBP1-70343-img0003.jpg)
![Western Blot: TIRAP (TLR2 and TLR4) Antibody [NB300-990] - (1 ug/ml) staining of human liver lysate 35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Antibody-Western-Blot-NB300-990-img0005.jpg "Western Blot: TIRAP (TLR2 and TLR4) Antibody [NB300-990] - (1 ug/ml) staining of human liver lysate 35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: RP105/CD180 Antibody [NBP2-20204] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RP105-Antibody-Western-Blot-NBP2-20204-img0001.jpg "Western Blot: RP105/CD180 Antibody [NBP2-20204] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: RP105/CD180 Antibody [NBP2-20204] - Mahlarvu xenograft, using RP105 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RP105-Antibody-Immunohistochemistry-Paraffin-NBP2-20204-img0002.jpg)
![Flow Cytometry: RP105/CD180 Antibody [NBP2-20204] - Flow cytometry on Jurkat cells, staining with RP105 antibody at 1:100 dilution (blue) or rabbit IgG (black).](http://images.novusbio.com/fullsize/RP105-Antibody-Flow-Cytometry-NBP2-20204-img0003.jpg)