| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-24893 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-19775SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85500-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71021 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27345SS | |||
| Azide and BSA Free | NBP2-80793 | |||
![Western Blot: IL-1 beta/IL-1F2 Antibody [NB600-633] - Methylene blue (M) inhibits NLRP3 inflammasome formation in post-spinal cord injury (SCI) microglia compared to sham (V). Expression of pro-IL-1B and mature IL-1B in microglia sorted from post-SCI spinal cords. Representative Western blot images are shown in (B). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2017.00391/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NB600-633-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: IL-1 beta/IL-1F2 Antibody [NB600-633] - Methylene blue (M) inhibits NLRP3 inflammasome formation in post-spinal cord injury (SCI) microglia compared to sham (V). Expression of pro-IL-1B and mature IL-1B in microglia sorted from post-SCI spinal cords. Representative Western blot images are shown in (B). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2017.00391/full) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody [NB600-633] - Trabecular Meshwork (TM) region of pig eyes were stained with IL-1 beta antibody (red). Image from verified customer review.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Immunocytochemistry-Immunofluorescence-NB600-633-img0009.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody [NB600-633] - Analysis of: Human IL1beta antibody Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT Localization: cytoplasm Staining: Close up of medullary lymph node: positive staining in the cytoplasm of circulating macrophages. Neg Ctr (far right) normal rabbit IgG with pH 6.2 40X](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Immunohistochemistry-NB600-633-img0010.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NB600-633] - Analysis of IL-1 beta in Rhesus fetal membrane using anti-IL-1 beta antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NB600-633-img0005.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NB600-633] - Antibody was used at a 1:200 dilution incubated 1h at room temperature to detect dog IL-1b by Western blot. Lane 1 and 2 were loaded with 2.5 ug and 500 ng of dog IL-1b respectively. The molecular weight of the detected band is estimated by comparison to molecular weight markers (not shown). Detection occurred using a 1:3,000 dilution of IRDye (TM) 800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NB600-633-img0006.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NB600-633] - pro-IL-1beta in glioblastoma cells. Image from verified customer review.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NB600-633-img0008.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NB600-633] - Lane 1: Human IL-1Beta. Load: 5 ng per lane. Primary antibody: Human IL-1Beta antibody at 1:2,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Human IL-1Beta. Other band(s): Unspecific band at 35 kDa.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NB600-633-img0011.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NB600-633] - Methylene blue inhibits inflammasome formation in cultured microglia. (A) Expression of Pro-Caspase-1, Caspase-1 p20, pro-IL-1 beta & mature IL-1 beta in microglia with or without stimulation in the presence or absence of methylene blue. Left: representative Western blot images. Right: statistics. N = 5 per group. The (B) expression of NLRP3, NLRC4 & Aim2 in microglia. Left: representative Western blot images. Right: statistics. N = 4 per group. (C) Co-immunoprecipitation assay showing the binding of ASC to NLRP3 or NLRC4. Left: representative Western blot images. Right: statistics. Un: no stimulation. LPS+ATP: stimulation with LPS followed by ATP treatment. V: vehicle; M: 500 nM methylene blue. IP: immunoprecipitation with the antibody against indicated protein. IB: detection of indicated protein. To analyze the relative expression of target proteins, the band intensities of target proteins were normalized to corresponding band intensities of alpha -Tubulin, followed by calculating the expression in other groups relative to “Un (V)” group. N = 4 per group. *p < 0.05; **p < 0.01; ***p < 0.001 in comparison with unstimulated cells of the vehicle group. #p < 0.05; ##p < 0.01 in comparison with stimulated cells of the vehicle group. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2017.00391/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-633_rabbit-polyclonal-il-1-beta-il-1f2-antibody-310202416165633.jpg)
![Western Blot: Rabbit Polyclonal IL-1 beta/IL-1F2 Antibody [NB600-633] - IL-1 beta/IL-1F2 Western blot of liver homogenate from control (1) and alcohol-fed mice (2). Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb600-633_rabbit-polyclonal-il-1-beta-il-1f2-antibody-western-blot-25112024153834..png)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/fullsize/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/fullsize/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - PBMCs fixed and permeabilized using the Novus intracellular staining kit and stained with 1 ug of TLR7-PE conjugate (red) or rabbit IgG-PE conjugate isotype control (NBP2-24983, green). Image using the PE form of this antibody.](http://images.novusbio.com/fullsize/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0002.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular analysis of TLR1 in human PBMCs (lymphocyte-gated) using this antibody. Shaded histogram represents cells alone, green represents isotype control , red represents TLR1 antibody. TLR intracellular flow kit was used for this test. Image using the Alexa Fluor 488 form of this antibody.](http://images.novusbio.com/fullsize/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0003.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Analysis of TLR7 in Ramos cells using 2 ug of NBP2-24892. Shaded histogram represents FITC-conjugated rabbit IgG isotype control ; red represents anti-TLR7 antibody. Image using the FITC form of this antibody.](http://images.novusbio.com/fullsize/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0004.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - FLOW analysis of Mouse CD4+ T cells that were stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. PE conjugated Glut1 antibody stained cells (NB110-39113PE, Orange) were included as positive control which were compared with Rb IgG PE, NBP2-24983 as Isotype Control (Blue) as well as with fluorescence minus one/FMO control (Red) [Image submitted by Verified Customer - see Review for Protocol Details]. Image using the PE form of this antibody.](http://images.novusbio.com/fullsize/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Rabbit IgG Isotype Control [NBP2-24891] - The expression & localization of PRCP in the human atherosclerotic plaque. (A) Immunohistochemical staining of PRCP (brown color) in a carotid endarterectomy sample. Scale bars, 500 µm (left) & 50 µm (right). (B) A consecutive section of the carotid sample was immunofluorescently stained for PRCP (red) & Mac-2 (green), & counterstained with DAPI. Cells that clearly express both PRCP & Mac-2 are indicated by white arrows. Scale bars, 500 µm (left) & 50 µm (right). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30305673), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-24891_rabbit-igg-isotype-control-31020241539599.jpg)
![Immunohistochemistry: Rabbit IgG Isotype Control [NBP2-24891] - alpha -melanocyte stimulating hormone ( alpha -MSH) is expressed by plaque macrophages in human atheroma. (A) alpha -MSH immunostaining (brown color) of carotid endarterectomy sample. Scale bars, 200 µm (left) & 50 µm (right). (B) A consecutive section of the carotid sample was immunofluorescently stained for alpha -MSH (red) & Mac-2 (green), & counterstained with DAPI. Cells that clearly express both alpha -MSH & Mac-2 are indicated by white arrows. Scale bars, 200 µm (left) & 50 µm (right). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30305673), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-24891_rabbit-igg-isotype-control-310202415392523.jpg)
![Immunohistochemistry-Frozen: Rabbit IgG Isotype Control [NB810-56910] - Mouse ear skin (aceton-fixed frozen section) stained with rabbit IgG isotype control, which was used as a negative control. Image from verified customer review.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Immunohistochemistry-Frozen-NB810-56910-img0012.jpg "Immunohistochemistry-Frozen: Rabbit IgG Isotype Control [NB810-56910] - Mouse ear skin (aceton-fixed frozen section) stained with rabbit IgG isotype control, which was used as a negative control. Image from verified customer review.")
![Immunohistochemistry-Frozen: Rabbit IgG Isotype Control [NB810-56910] - Mouse ear skin (aceton-fixed frozen section) stained with rabbit IgG isotype control, which was used as a negative control. Image from verified customer review.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Immunohistochemistry-Frozen-NB810-56910-img0011.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-19775] - Analysis of IL1 beta Antibody in extracts from HeLa cells.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-19775-img0004.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-19775] - Analysis of IL1 beta Antibody in extracts from HeLa cells.")
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody - BSA Free [NBP1-19775] - In situ expression of IL-6 in glomerular and tubulointerstitial compartments in patients with diabetic nephropathy DN (right) and control group (left). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32723296/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody---BSA-Free-Immunohistochemistry-NBP1-19775-img0006.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody [NBP1-19775] - Staining in the cytoplasm of villi and in the crypt cells of human lung carcinoma tissue.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Immunohistochemistry-NBP1-19775-img0005.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the HRP conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1 Beta Load: 50 ug per Detection of Lane Secondary antibody: IL1 beta Antibody Peroxidase conjugated at 1:1,000 for 30 min at RT Block for30 min RT Predicted/Observed size: 18 kDa, 18 kDa.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-42767-img0012.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the HRP conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1 Beta Load: 50 ug per Detection of Lane Secondary antibody: IL1 beta Antibody Peroxidase conjugated at 1:1,000 for 30 min at RT Block for30 min RT Predicted/Observed size: 18 kDa, 18 kDa.")
![Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Staining of mouse carotid artery. A) No Ab staining of WT uninjured Ms. B) IL-1 beta staining of cells after surgical injury of tissue. C) No Ab staining of injured tissue from an IL-1 beta KO.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-42767-img0004.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis of Mouse Embryonic Kidney Tissue: Mouse Embryonic Kidney Fixation: FFPE buffered formalin 10% conc Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 2ug/ml 1.5 hour @ room T Secondary Ab: MACH 1 HRP POLYMER 1:50 45C RT](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Immunohistochemistry-NBP1-42767-img0010.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the Biotin conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1beta Recombinant Protein. Load: 50 ng per lane. Used at a dilution of 1:1,000 overnight at 4C.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-42767-img0008.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Detects 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1Beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in western blot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1Beta precursor molecule. Denatured IL-1Beta will have a 18 kDa band.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-42767-img0011.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24893] - Intracellular analysis of mouse TLR 6 polyclonal antibody (Red peak), rabbit isotype control (Green peak), RAW cells (Purple peak).Two micrograms of antibodies were used. Goat anti-rabbit FITC was used as secondary antibody.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24893-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24893] - Intracellular analysis of mouse TLR 6 polyclonal antibody (Red peak), rabbit isotype control (Green peak), RAW cells (Purple peak).Two micrograms of antibodies were used. Goat anti-rabbit FITC was used as secondary antibody.")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24893] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24893-img0002.jpg)
![Western Blot: Rabbit IgG Isotype Control [NBP2-36463] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Western-Blot-NBP2-36463-img0028.jpg "Western Blot: Rabbit IgG Isotype Control [NBP2-36463] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.")
![Flow (Intracellular): Rabbit IgG Isotype Control [NBP2-36463] - Intracellular staining for GLI1, GLI2 and GLI3 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using a secondary anti-rabbit IgG PE conjugated antibody. Image courtesy of Sherine Elsawa, Northern Illinois University.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Flow-Intracellular-NBP2-36463-img0007.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP2-36463] - Lane 1: Non-reduced Rabbit IgG Whole Molecule. Lane 2: 5uL OPAL Pre-stained Marker. Lane 3: Reduced Rabbit IgG Whole Molecule. Load: 1ug per lane. Predicted/Observed size: Non-reduced at 150-170 kDa , Reduced at 55, 25 kDa.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-SDS-Page-NBP2-36463-img0018.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP2-36463] - SDS-Page of Rabbit IgG. Lane 1: Rabbit IgG - Non-Reduced. Lane 2: Rabbit IgG - Reduced. Load: 1.0 ug per lane. Predicted/Observed Size: Non-reduced - 130 kDa, Reduced - 55 and 28 kDa for Rabbit IgG. Other Band(s): None.](http://images.novusbio.com/fullsize/Rabbit IgG Isotype Control-SDS-Page-NBP2-36463-img0030.jpg)
![SDS-Page: Rabbit IgG Isotype Control [NBP2-36463] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-SDS-Page-NBP2-36463-img0029.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody [NBP1-85500] - Staining in human skin and skeletal muscle tissues using anti-PTGS1 antibody. Corresponding PTGS1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85500-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: COX-1 Antibody [NBP1-85500] - Staining in human skin and skeletal muscle tissues using anti-PTGS1 antibody. Corresponding PTGS1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: COX-1 Antibody [NBP1-85500] - Analysis in human cell line HELA.](http://images.novusbio.com/fullsize/COX-1-Antibody-Western-Blot-NBP1-85500-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: COX-1 Antibody [NBP1-85500] - Staining of human cell line BJ shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85500-img0009.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody [NBP1-85500] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85500-img0010.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody [NBP1-85500] - Staining of human skin shows high expression.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85500-img0011.jpg)
![Simple Western: COX-1 Antibody [NBP1-85500] - Lane view shows one specific signal (~70kDa) in lysates prepared from the mouse tail vein. Image from a verified customer review.](http://images.novusbio.com/fullsize/COX-1-Antibody-Simple-Western-NBP1-85500-img0013.jpg)
![Western Blot: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of extracts (10ug) from 5 different cell lines by using anti-IL1A monoclonal antibody. (1:200)](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Western-Blot-NBP2-45400-img0008.jpg "Western Blot: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of extracts (10ug) from 5 different cell lines by using anti-IL1A monoclonal antibody. (1:200)")
![Immunocytochemistry/Immunofluorescence: IL-1 alpha/IL-1F1 Antibody (OTI2F8) [NBP2-45400] - IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY IL-1 alpha.](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Immunofluorescence-NBP2-45400-img0001.jpg)
![Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Immunohistochemistry-NBP2-45400-img0006.jpg)
![Western Blot: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of HEK293T cells lysate (5 ug, left lane) and full length human recombinant protein of human IL-1 alpha (NP_000566) produced in Analysis of HEK293T cell (5 ug, right lane).](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Western-Blot-NBP2-45400-img0007.jpg)
![Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Immunohistochemistry-NBP2-45400-img0002.jpg)
![Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Immunohistochemistry-NBP2-45400-img0003.jpg)
![Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Immunohistochemistry-NBP2-45400-img0004.jpg)
![Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/IL-1-alpha-IL-1F1-Antibody-2F8-Immunohistochemistry-NBP2-45400-img0005.jpg)
![Western Blot: COX-1 Antibody [NB100-867] - Staining (1ug/ml) of U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/COX-1-Antibody-Western-Blot-NB100-867-img0010.jpg "Western Blot: COX-1 Antibody [NB100-867] - Staining (1ug/ml) of U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: COX-1 Antibody [NB100-867] - Staining (3.8µg/ml) of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-Staining.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NB100-867-img0009.jpg)
![Immunohistochemistry: COX-1 Antibody [NB100-867] - Immunohistochemistry and Virtual Channel confocal microscopy of IL-4 and IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization of advillin (green), DCLK1 (red) and PTGS1/COX-1 (magenta) within the same cells identified as tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-020-65469-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-NB100-867-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - Intestinal tuft cells express advillin. (a) Immunohistochemistry of paraffin-embedded tissue from distal ileum of C57BL/6 J mice using advillin (green) & DCLK1 (red) antibodies. Nuclei are counter stained with DAPI. Both proteins co-localize in the same cell. Note that not all DCLK1 positive cells are positive for advillin expression. Right panel shows higher magnification of boxed area & shows co-localization of DCLK1 & advillin in a tuft cell. (b) Immunohistochemistry of paraffin embedded tissue from distal ileum of C57BL/6 J mice using advillin (green) & PTGS1 (red) antibodies. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area & co-localization of PTGS1 & advillin in a tuft cell. (c) Immunohistochemistry of cryopreserved tissue from distal ileum of C57BL/6 J mice using Alexa Fluor 568 Phalloidin (red) & advillin (green). Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area & co-localization of advillin & F-actin at the apical surface of a tuft cell. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-31020241628497.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - In intestinal enteroids, tuft cells express advillin. (a) Immuno-histochemistry of enteroids from distal ileum of C57BL/6 J mice shows tuft cell hyperplasia 72 hours post IL-4 & IL-13 treatment. Control refers to untreated enteroids from C57BL/6 J mice. Advillin (green) & DCLK1 (red) co-localization was used to identify tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of the boxed area. (b) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization in tuft cells of advillin (green) & DCLK1 (red) in the upper panel; & advillin & PTGS1 (red) in the lower panel. Nuclei were counter stained with DAPI. Right panels show higher magnification of boxed area. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-31020241628492.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - Tuft cells positive for advillin are negative for villin. (a) Immunohistochemistry & Virtual Channel confocal microscopy of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization of advillin (green), DCLK1 (red) & PTGS1 (magenta) within the same cells identified as tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. (b) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice show co-localization in tuft cells of advillin (green) with F-actin (red) in the upper panel; & co-localization of advillin (red) & tubulin (green) in the lower panel. Nuclei were counter stained with DAPI. Right panel shows higher magnification of boxed area. Note the co-localization of advillin to the apical tuft that terminates near the perinuclear region of the cells. Advillin appears in vesiculated structures that co-localize with tubulin near the lateral cell surface & in the cytoplasm. (c) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice show the absence of villin (red) from advillin (green) expressing tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-310202416284923.jpg)
![Western Blot: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of COX-1 on different lysates with Rabbit anti-COX-1 antibody at 1/1,000 dilution. Lane 1: C2C12 cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 69 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Western-Blot-NBP2-67643-img0011.jpg "Western Blot: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of COX-1 on different lysates with Rabbit anti-COX-1 antibody at 1/1,000 dilution. Lane 1: C2C12 cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 69 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: COX-1 Antibody (SC68-05) [NBP2-67643] - Staining COX1 in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunocytochemistry-Immunofluorescence-NBP2-67643-img0010.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-COX-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunohistochemistry-Paraffin-NBP2-67643-img0012.jpg)
![Flow Cytometry: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of Hela cells with COX1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Flow-Cytometry-NBP2-67643-img0001.jpg)
![Western Blot: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of COX1 on different lysates using anti-COX1 antibody at 1/1,000 dilution. Positive control: Lane 1: C2C12 Lane 2: A431](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Western-Blot-NBP2-67643-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: COX-1 Antibody (SC68-05) [NBP2-67643] - Staining COX1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunocytochemistry-Immunofluorescence-NBP2-67643-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: COX-1 Antibody (SC68-05) [NBP2-67643] - Staining COX1 in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunocytochemistry-Immunofluorescence-NBP2-67643-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: COX-1 Antibody (SC68-05) [NBP2-67643] - Staining COX1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunocytochemistry-Immunofluorescence-NBP2-67643-img0009.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of paraffin-embedded human breast cancer tissue using anti-COX1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunohistochemistry-Paraffin-NBP2-67643-img0004.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of paraffin-embedded mouse brain tissue using anti-COX1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunohistochemistry-Paraffin-NBP2-67643-img0005.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of paraffin-embedded mouse skin tissue using anti-COX1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunohistochemistry-Paraffin-NBP2-67643-img0006.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of paraffin-embedded mouse stomach tissue using anti-COX1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunohistochemistry-Paraffin-NBP2-67643-img0007.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in A) full-length recombinant protein and B) human ThP1 lysate using Interleukin-1 beta antibody at 1 ug/ml and 5 ug/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Western-Blot-NBP2-27345-img0008.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in A) full-length recombinant protein and B) human ThP1 lysate using Interleukin-1 beta antibody at 1 ug/ml and 5 ug/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Intracellular staining of Interleukin-1 beta positive T cells (upper panel) & monocytes (lower panel) from stimulated PBMC (with a cocktail of TLR ligands and PMA/IM for 6h) using Interleukin-1 beta antibody at 0.5 ug/10^6 cells.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Flow-Cytometry-NBP2-27345-img0006.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of full length human IL-1 beta recombinant protein (10ng/well) using IL1 beta Antibody (NBP2-27345) at 1:1000 (left lane) and 1:100 (right lane).](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Western-Blot-NBP2-27345-img0009.jpg)
![Immunohistochemistry-Paraffin: IL-1 beta/IL-1F2 Antibody (43N3D8) [NBP2-27345] - Analysis of Interleukin-1 beta in human kidney tissue using Interleukin-1 beta antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N3D8-Immunohistochemistry-Paraffin-NBP2-27345-img0007.jpg)