| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77078SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56437SS | |||
| BSA Free | NB100-56437 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36489SS | |||
| Azide and BSA Free | NBP2-80743 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87466-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24640SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77067SS | |||
![Immunocytochemistry/Immunofluorescence: GPR81 Antibody [NLS2095] - LPS increases glycolysis as well as lactate production by BM neutrophils. MCT4, MCT1, and GPR81 (yellow) distribution on BM CD11b+ (green)/Ly6G+ (red) neutrophils visualized and quantified by ImageStream analysis. Images are from one representative experiment out of three. Scale bar indicates 7 um. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-020-17402-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunocytochemistry-Immunofluorescence-NLS2095-img0010.jpg "Immunocytochemistry/Immunofluorescence: GPR81 Antibody [NLS2095] - LPS increases glycolysis as well as lactate production by BM neutrophils. MCT4, MCT1, and GPR81 (yellow) distribution on BM CD11b+ (green)/Ly6G+ (red) neutrophils visualized and quantified by ImageStream analysis. Images are from one representative experiment out of three. Scale bar indicates 7 um. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-020-17402-2) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Brain, Substantia Nigra, pigmented and nonpigmented neurons.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0009.jpg)
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Analysis of anti-FKSG80 / GPR81 antibody with human anterior pituitary.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0002.jpg)
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Analysis of anti-FKSG80 / GPR81 antibody with human colon, carcinoma.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0004.jpg)
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Human Skin, Melanoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0005.jpg)
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Human Ovary, Carcinoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0006.jpg)
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Human pancreas, carcinoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0007.jpg)
![Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Breast, Carcinoma](http://images.novusbio.com/fullsize/GPR81-Antibody-Immunohistochemistry-Paraffin-NLS2095-img0008.jpg)
![Western Blot: GPR81 Antibody - BSA Free [NLS2095] - Stable knockdown of GPR81 in MDA-MB-231 cells. Using a lentiviral system, GPR81 was stably knocked down in MDA-MB-231 cells with GPR81-specific short hairpin RNA (shGPR81 #1) & compared with cells containing scrambled shRNA as a control (shNT). Knockdown of GPR81 was confirmed by (a) western blotting & (b) RT-qPCR. The GPR81, MCT1, & MCT4 mRNA levels in the shGPR81 #1 cells are presented as the fold increase compared with those in shNT cells. Data are presented as the means ± s.d. (n = 3/group). **p < 0.01; Student’s t-test. (c) Lactate production in shNT & shGPR81 #1 cells. The cells were cultured for 24 h & lactate concentrations in the supernatants were determined (n = 3/group). **p < 0.01 (vs. shNT cells); Student’s t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35428832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls2095_rabbit-gpr81-pab-bsa-free-310202415334917.jpg)
![Western Blot: GPR81 Antibody - BSA Free [NLS2095] - Expression of GPR81 in breast cancer cells. (a) Western blot analysis of GPR81 in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). Cell lysates were analyzed by immunoblotting using anti-GPR81, anti-MCT4, anti-MCT1, & anti-beta -actin antibodies. (b) Immunocytochemical analysis of GPR81 expression in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). MCF-10A, MCF-7 & MDA-MB-231 cells were incubated with polyclonal antibodies against GPR81 followed by Alexa Fluor Plus 488-conjugated secondary antibody & visualized under a fluorescence microscope. Na/K ATPase was used as a cell membrane marker & the nuclei were stained with DAPI. Scale bar 10 μm. (c) MDA-MB-231 cells were cultured in the presence & absence of 20 mM lactate for 48 h, & cell lysates were analyzed by immunoblotting using anti-GPR81 & anti-beta -actin antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35428832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls2095_rabbit-gpr81-pab-bsa-free-31020241613628.jpg)
![Western Blot: GPR81 Antibody - BSA Free [NLS2095] - Expression of GPR81 in breast cancer cells. (a) Western blot analysis of GPR81 in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). Cell lysates were analyzed by immunoblotting using anti-GPR81, anti-MCT4, anti-MCT1, & anti-beta -actin antibodies. (b) Immunocytochemical analysis of GPR81 expression in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). MCF-10A, MCF-7 & MDA-MB-231 cells were incubated with polyclonal antibodies against GPR81 followed by Alexa Fluor Plus 488-conjugated secondary antibody & visualized under a fluorescence microscope. Na/K ATPase was used as a cell membrane marker & the nuclei were stained with DAPI. Scale bar 10 μm. (c) MDA-MB-231 cells were cultured in the presence & absence of 20 mM lactate for 48 h, & cell lysates were analyzed by immunoblotting using anti-GPR81 & anti-beta -actin antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35428832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls2095_rabbit-gpr81-pab-bsa-free-310202416114765.jpg)
![Immunocytochemistry/Immunofluorescence: CX3CR1 Antibody [NBP1-76949] - Staining of Human Heart cells with CX3CR1.](http://images.novusbio.com/fullsize/CX3CR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76949-img0004.jpg "Immunocytochemistry/Immunofluorescence: CX3CR1 Antibody [NBP1-76949] - Staining of Human Heart cells with CX3CR1.")
![Immunohistochemistry-Paraffin: CX3CR1 Antibody [NBP1-76949] - Human tonsil, pH9 antigen retrieval. Image from verified customer review.](http://images.novusbio.com/fullsize/CX3CR1-Antibody-Immunohistochemistry-Paraffin-NBP1-76949-img0005.jpg)
![Immunohistochemistry-Paraffin: CX3CR1 Antibody [NBP1-76949] - Human heart tissue using CX3CR1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/CX3CR1-Antibody-Immunohistochemistry-Paraffin-NBP1-76949-img0002.jpg)
![Western Blot: P2Y12/P2RY12 Antibody [NBP1-78249] - Antibody dilution 1:500, MW is 41 kDa](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Western-Blot-NBP1-78249-img0002.jpg "Western Blot: P2Y12/P2RY12 Antibody [NBP1-78249] - Antibody dilution 1:500, MW is 41 kDa")
![Immunocytochemistry/Immunofluorescence: P2Y12/P2RY12 Antibody [NBP1-78249] - SR8278 induces microglial process extension and expression of P2Y12R and Bmal1. SR8278 (20 uM) recovered the fluorescence intensity of Bmal1 and P2Y12R and increased microglial process length in either the absence or presence of fAbeta1-42 (P2Y12R in red and Bmal1 in green). The graph shows the average length of the longest microglial processes from the 46 microglia in each group. ***p < .001 compared to the vehicle-treated group and ###p < .001 compared to the fAbeta-treated group. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1111/acel.13078) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78249-img0007.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249] - Mouse brain, 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxlyin QS (blue) counterstain. 40X magnification, FFPE section.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP1-78249-img0006.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249] - Rat brain, 1:100 dilution.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP1-78249-img0003.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249] - Rat spinal cord. Dilution 1:100.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP1-78249-img0004.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249] - Rat spinal cord. Dilution 1:100.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP1-78249-img0005.jpg)
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Brain Cerebellum Purkinje Neuron](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NLS1380-img0010.jpg "Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Brain Cerebellum Purkinje Neuron")
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Analysis of anti-CXCR4 antibody with human tonsil.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NLS1380-img0004.jpg)
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Analysis of anti-CXCR4 antibody with human colon, carcinoma.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NLS1380-img0006.jpg)
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Human Lymph Node, Non-Hodgkins Lymphoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NLS1380-img0007.jpg)
![Immunohistochemistry-Frozen: CXCR4 Antibody [NLS1380] - Rat brain tissue sections. Rat Nucleus Accumbens treated with MDPV. Images taken with fluorescent microscope. Primary antibody was diluted to 1:200. Secondary antibody used was donkey anti-Rabbit Alexa Fluor 594 at 1:500. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Frozen-NLS1380-img0008.jpg)
![Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Lung, small cell carcinoma](http://images.novusbio.com/fullsize/CXCR4-Antibody-Immunohistochemistry-Paraffin-NLS1380-img0009.jpg)
![Western Blot: AGTR-1 Antibody [NBP1-77078] - Effects of Ang(1-7) and/or A-779 treatments to the sham and OVX animals for 6 weeks on the expressions of RAS different proteins and osteoclastogenesis modulating factors in the femoral bone heads. Western blot analysis bands showing the expressions of AngII, Ang(1-7), AT1R, AT2R, ACE, ACE-2, MasR, RANKL and OPG. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-02570-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AGTR-1-Antibody-Western-Blot-NBP1-77078-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: AGTR-1 Antibody [NBP1-77078] - Effects of Ang(1-7) and/or A-779 treatments to the sham and OVX animals for 6 weeks on the expressions of RAS different proteins and osteoclastogenesis modulating factors in the femoral bone heads. Western blot analysis bands showing the expressions of AngII, Ang(1-7), AT1R, AT2R, ACE, ACE-2, MasR, RANKL and OPG. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-02570-x) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: AGTR-1 Antibody [NBP1-77078] - Mouse kidney tissue lysate with Angiotensin II Type 1 Receptor antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/AGTR-1-Antibody-Western-Blot-NBP1-77078-img0006.jpg)
![Immunohistochemistry: AGTR-1 Antibody - BSA Free [NBP1-77078] - Immunohistochemistry of AGTR-1 in mouse kidney tissue with AGTR-1 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/nbp1-77078_rabbit-polyclonal-agtr-1-antibody-2711202419333426.gif)
![Immunocytochemistry/ Immunofluorescence: AGTR-1 Antibody - BSA Free [NBP1-77078] - Immunofluorescence of AGTR-1 in Mouse Kidney cells with AGTR-1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77078_rabbit-polyclonal-agtr-1-antibody-2711202420212376.gif)
![Immunohistochemistry: AGTR-1 Antibody - BSA Free [NBP1-77078] - Immunohistochemistry of AGTR-1 in mouse kidney tissue with AGTR-1 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77078_rabbit-polyclonal-agtr-1-antibody-2711202420312953.jpg)
![Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Western blot shows lysates of Jurkat human T lymphocyte parental cell line and CXCR4 knockout (KO) Jurkat cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CXCR4 Polyclonal Antibody (Catalog # NB100-56437) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CXCR4 at approximately 38 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in the knockout Jurkat cell line. This experiment was conducted under reducing conditions. Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Western-Blot-NBP2-24862-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Western blot shows lysates of Jurkat human T lymphocyte parental cell line and CXCR4 knockout (KO) Jurkat cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CXCR4 Polyclonal Antibody (Catalog # NB100-56437) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CXCR4 at approximately 38 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in the knockout Jurkat cell line. This experiment was conducted under reducing conditions. Image using the standard format of this product.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CXCR4 Antibody - Azide Free [NBP2-24862] - Jurkat cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-CXCR4 (NB100-56437) at a 1:25 dilution for 1 hour at room temperature and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-24862-img0002.jpg)
![Immunohistochemistry: CXCR4 Antibody - Azide Free [NBP2-24862] - CXCR4 was detected in immersion sections of human tonsil using rabbit anti-human antibody (Catalog # NB100-56437) at 1:300 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-rabbit HRP polymer detection reagent with DAB chromogen (brown) and counterstained with hematoxylin (blue). Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Immunohistochemistry-NBP2-24862-img0003.jpg)
![Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Analysis of 15 ug of whole cell lysate from HeLa cells with anti-CXCR4 at 1 ug/ml.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Western-Blot-NBP2-24862-img0001.jpg)
![Western Blot: DARC Antibody [NB100-2421] - DARC (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/DARC-Antibody-Western-Blot-NB100-2421-img0007.jpg "Western Blot: DARC Antibody [NB100-2421] - DARC (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421] - Staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer Ph 6, AP-staining.](http://images.novusbio.com/fullsize/DARC-Antibody-Immunohistochemistry-Paraffin-NB100-2421-img0009.jpg)
![Flow Cytometry: DARC Antibody [NB100-2421] - analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/DARC-Antibody-Flow-Cytometry-NB100-2421-img0010.jpg)
![Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421] - DARC (3ug/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/DARC-Antibody-Immunohistochemistry-Paraffin-NB100-2421-img0008.jpg)
![Western Blot: Dopamine D1R/DRD1 Antibody [NBP2-16213] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/Dopamine-D1-R-DRD1-Antibody-Western-Blot-NBP2-16213-img0004.jpg "Western Blot: Dopamine D1R/DRD1 Antibody [NBP2-16213] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE diluted at 1:1000")
![Immunocytochemistry/Immunofluorescence: Dopamine D1R/DRD1 Antibody [NBP2-16213] - DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine Receptor D1 stained by Dopamine Receptor D1 antibody [C2C3], C-term diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Dopamine-D1R-DRD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16213-img0006.jpg)
![Immunohistochemistry-Frozen: Dopamine D1R/DRD1 Antibody [NBP2-16213] - Frozen-sectioned mouse cerebral cortex. Green: Dopamine Receptor D1 stained by Dopamine Receptor D1 antibody [C2C3], C-term diluted at 1:250.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Dopamine-D1R-DRD1-Antibody-Immunohistochemistry-Frozen-NBP2-16213-img0005.jpg)
![Immunohistochemistry-Paraffin: Dopamine D1R/DRD1 Antibody [NBP2-16213] - U373 xenograft, using Dopamine Receptor D1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Dopamine-D1-R-DRD1-Antibody-Immunohistochemistry-Paraffin-NBP2-16213-img0003.jpg)
![Western Blot: FSH R Antibody (6E8.2F5) [NBP2-36489] - WB analysis of (A) 15kDa partial recombinant Human FSHR protein and (B) lysate of HepG2 cells using FSH R antibody (clone 6E8.2F5) at 3ug/ml concentration.](http://images.novusbio.com/fullsize/FSH-R-Antibody-6E8-2F5-Western-Blot-NBP2-36489-img0004.jpg "Western Blot: FSH R Antibody (6E8.2F5) [NBP2-36489] - WB analysis of (A) 15kDa partial recombinant Human FSHR protein and (B) lysate of HepG2 cells using FSH R antibody (clone 6E8.2F5) at 3ug/ml concentration.")
![Immunohistochemistry-Paraffin: FSH R Antibody (6E8.2F5) [NBP2-36489] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human rectal adenocarcinoma using FSH R antibody (clone 6E8.2F5) at 5 ug/ml concentration. The columnar cells depicted strong membranous staining with weaker cytoplasmic signal for FSHR protein.](http://images.novusbio.com/fullsize/FSH-R-Antibody-6E8-2F5-Immunohistochemistry-Paraffin-NBP2-36489-img0003.jpg)
![Immunohistochemistry-Paraffin: FSH R Antibody (6E8.2F5) [NBP2-36489] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human kidney using FSH R antibody (clone 6E8.2F5) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/FSH-R-Antibody-6E8-2F5-Immunohistochemistry-Paraffin-NBP2-36489-img0001.jpg)
![Immunohistochemistry-Paraffin: FSH R Antibody (6E8.2F5) [NBP2-36489] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human placenta using FSH R antibody (clone 6E8.2F5) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/FSH-R-Antibody-6E8-2F5-Immunohistochemistry-Paraffin-NBP2-36489-img0002.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining in human cerebral cortex and pancreas tissues using anti-CHRM1 antibody. Corresponding CHRM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87466-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining in human cerebral cortex and pancreas tissues using anti-CHRM1 antibody. Corresponding CHRM1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining of dissociated rat hippocampal culture. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunohistochemistry-NBP1-87466-img0003.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87466-img0007.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87466-img0008.jpg)
![Western Blot: GPR35 Antibody [NBP2-24640] - Analysis of G Protein-Coupled Receptor GPR35 in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide 3) RAW using NBP2-24640 at 0.5 ug/ml and 1.0 ug/ml, respectively.](http://images.novusbio.com/fullsize/GPR35-Antibody-Western-Blot-NBP2-24640-img0001.jpg "Western Blot: GPR35 Antibody [NBP2-24640] - Analysis of G Protein-Coupled Receptor GPR35 in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide 3) RAW using NBP2-24640 at 0.5 ug/ml and 1.0 ug/ml, respectively.")
![Immunohistochemistry-Paraffin: GPR35 Antibody [NBP2-24640] - Analysis of GPR35 in paraffin-embedded formalin-fixed MCF7cells using an isotype control (top left) and NBP2-24640 (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/GPR35-Antibody-Immunohistochemistry-Paraffin-NBP2-24640-img0002.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: 15 ug of lysates per lane. Antibodies: NBP1-7706 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0013.jpg "Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: 15 ug of lysates per lane. Antibodies: NBP1-7706 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP")
![Immunohistochemistry: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in Human Spleen. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CXCR4 antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A Goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202420324853.gif)
![<b>Genetic Strategies Validation. </b>Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Astrocytes from wild-type or CXCR4 knockout mice were stained with primary antibodies against CXCR4 and FITClabeled secondary antibodies, and subsequently subjected to flow cytometry. CXCR4-/- astrocytes (red) showed loss of CXCR4 cell-surface expression compared with wild-type cells (black).](http://images.novusbio.com/fullsize/CXCR4-Antibody-Flow-Cytometry-NBP1-77067-img0010.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa whole cell lysate with CXCR4 antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0001.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Recombinant Protein Test: Loading: CXCR4 partial recombinant protein (H00007852-Q01). Lane 1: Anti-CXCR4 antibody (0.1 ug/mL) 1 h incubation at RT in 5% NFDM/TBST. Lane 2: Coomassie blue staining.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0007.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0008.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - U87MG and U87MG-CXCR4 extracts were included as negative and positive controls, respectively, for CXCR4 detection with anti-CXCR4 antibodies.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0009.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: Lysates/proteins at 20 ug per lane. Antibodies: NBP1-7706 (2 ug/mL) or NBP1-76480 (2 ug/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: NBP1-7706 (2 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0012.jpg)
![Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Analysis of HeLa cells using CXCR4 antibody at 0.1 ug/ml. Purple: cells without staining, Green: Isotype control. Red : CXCR4 antibody.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Flow-Cytometry-NBP1-77067-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-CXCR4 antibody at 2 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202419313214.gif)
![Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cells.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202420414588.jpg)