GPR55 Antibody

Images

 
Immunohistochemistry-Frozen: GPR55 Antibody [NB110-55498] - Cryosection of canine cervical dorsal root ganglion in which satellite glial cells and some sensory neurons expressed GPR55 immunoreactivity. Image from ...read more
Immunohistochemistry-Paraffin: GPR55 Antibody [NB110-55498] - Analysis of anti-GPR55 antibody with human pancreas, islet of langerhans.

Product Details

Summary
Reactivity Hu, Ca, Pm, PmSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

GPR55 Antibody Summary

Immunogen
Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human GPR55.
Epitope
Third cytoplasmic loop of human
Specificity
Human GPR55. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Species
Primate (95%), Monkey (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPR55
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
Use in WB reported in scientific literature (PMID:31608295). GPR55 antibody validated for IHC-F from a verified customer review.
Control
GPR55 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB110-55498 in the following applications:

Publications
Read Publications using
NB110-55498 in the following applications:

  • WB
    2 publications

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (95%), Marmoset (95%), Bovine (85%), Mouse (80%), Equine (80%), Porcine (80%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GPR55 Antibody

  • G protein-coupled receptor 55
  • Gm218
  • GPR55
  • G-protein coupled receptor 55

Background

G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPCR GPR55 is a member of this family (subfamily Orphan-A). It has been reported to be expressed in the brain, where mRNA transcripts were detected in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain. ESTs have been isolated from liver/spleen libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GPR55 Antibody (NB110-55498)(2)

We have publications tested in 1 confirmed species: Canine.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
All Applications
Filter By Species
Canine
(2)
All Species
Showing Publications 1 - 2 of 2.
Publications using NB110-55498 Applications Species
Chiocchetti R, Galiazzo G, Tagliavia C Cellular distribution of canonical and putative cannabinoid receptors in canine cervical dorsal root ganglia Frontiers in Veterinary Science Sep 19 2019 (WB, Canine) WB Canine
Chiocchetti R, Galiazzo G, Tagliavia C et al. Corrigendum: Cellular Distribution of Canonical and Putative Cannabinoid Receptors in Canine Cervical Dorsal Root Ganglia Front. Vet. Sci. 2019 Oct 31 [PMID: 31608295] (WB, Canine) WB Canine

Review for GPR55 Antibody (NB110-55498) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Canine.

Reviews using NB110-55498:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Canine
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen GPR55 NB110-55498
Enlarge
5
reviewed by:
Anonymous
IHC-Fr Canine 07/08/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedCanine sensory neurons,Canine peripheral sensory neurons
SpeciesCanine
Lot39888

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GPR55 Antibody (NB110-55498). (Showing 1 - 2 of 2 FAQs).

  1. Do you have any data about the use of the following antibody in Immunofluorescence and Confocal Microscopy:
    • We have only validated product NB110-55498 for use in IHC Paraffin. Please see our images on the datasheet. The recommended dilution for this antibody in IHC-P is 5 ug/ml.
  2. I'm interested in one of your products: GPR55 Antibody (NB110-55498). I'd like to know if it has ever been used in non permeabilised cells, indicating that it can efficiently recognize an extracellular epitope.
    • We have done validation of this product in IHC-P of pancreas as well as spleen tissues after heat-induced antigen retrieval and yes, permeabilization was executed before blocking/primary antibody incubation step in the protocol. Have you seen our another GPR55 antibody (NLS6817)? This antibody has been generated employing an immunogen which was a synthetic peptide made to the 3rd extracellular domain of GPR55 protein. Because this antibody should technically be detecting extra-cellular domain of the target, you may try skipping permeabilization step for this antibody, however, you will still need to perform antigen retrieval.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional GPR55 Products

Array NB110-55498

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
07/08/2019
Application: IHC-Fr
Species: Canine

Bioinformatics

Gene Symbol GPR55
Entrez
Uniprot