GPR55 Antibody - BSA Free

Immunohistochemistry: GPR55 Antibody [NB110-55498] - Photomicrographs of cryosections of canine cervical (C8) dorsal root ganglion showing GPR55 (b-f) immunolabeling. (a-c) Arrows indicate the Neurotrace-labeled nuclei of satellite glial cells which showed bright GPR55 immunolabelling (c). White stars indicate unlabeled sensory neurons; open stars indicate empty spaces in which sensory neurons were no more evident. (d-f) White arrows indicate satellite glial cells which co-expressed bright GPR55- (d) and glial fibrillary acidic protein (GFAP) immunoreactivity. Stars indicate sensory neurons of different dimension, which expressed faint-to-moderate GPR55 immunoreactivity. Open arrow indicate autofluorescent pigment. Bar: 50 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fvets.2019.00313/full) licensed under a CC-BY licence.

Western Blot: GPR55 Antibody [NB110-55498] - Representative image of Western blots (WB) analysis showing the specificity of the primary antibody rabbit anti-G protein-coupled receptor 55 (GPR55). The antibody revealed a single band of expected molecular weight (~40 kDa). The images of the different immunoblots were slightly adjusted in brightness and contrast to match their backgrounds. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fvets.2019.00313/full) licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: GPR55 Antibody [NB110-55498] - Analysis of anti-GPR55 antibody with human pancreas, islet of langerhans.

Immunohistochemistry-Frozen: GPR55 Antibody [NB110-55498] - Cryosection of canine cervical dorsal root ganglion in which satellite glial cells and some sensory neurons expressed GPR55 immunoreactivity. Image submitted by a verified customer review.

• Reactivity: Hu, Ca, Pm, Pm
• Applications: WB, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GPR55 Antibody - BSA Free Summary

• Immunogen: Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human GPR55.
• Epitope: Third cytoplasmic loop of human
• Specificity: Human GPR55. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Primate (100%), Monkey (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPR55
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot
• Application Notes: Use in WB reported in scientific literature (PMID:31608295). GPR55 antibody validated for IHC-F from a verified customer review.
• Control:

  GPR55 Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (95%), Marmoset (95%), Bovine (85%), Mouse (80%), Equine (80%), Porcine (80%). Canine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

Product can be stored undiluted at 4C for up to 1 month.

Alternate Names for GPR55 Antibody - BSA Free

• G protein-coupled receptor 55
• Gm218
• GPR55
• G-protein coupled receptor 55

Background

G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPCR GPR55 is a member of this family (subfamily Orphan-A). It has been reported to be expressed in the brain, where mRNA transcripts were detected in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain. ESTs have been isolated from liver/spleen libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPR55 Antibody (NB110-55498)(1)

Publication using NB110-55498

• Chiocchetti R, Galiazzo G, Tagliavia C et al. Corrigendum: Cellular Distribution of Canonical and Putative Cannabinoid Receptors in Canine Cervical Dorsal Root Ganglia Front. Vet. Sci. 2019-10-31 [PMID: 31608295] (WB, Canine)

Review for GPR55 Antibody (NB110-55498) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Canine.

reviewed by: roberto Chiocchetti IHC-Fr Canine 07/08/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Canine peripheral sensory neurons,Canine sensory neurons
• Species: Canine
• Lot: 39888

FAQs for GPR55 Antibody (NB110-55498). (Showing 1 - 2 of 2 FAQ).

1. Do you have any data about the use of the following antibody in Immunofluorescence and Confocal Microscopy:
   • We have only validated product NB110-55498 for use in IHC Paraffin. Please see our images on the datasheet. The recommended dilution for this antibody in IHC-P is 5 ug/ml.
2. I'm interested in one of your products: GPR55 Antibody (NB110-55498). I'd like to know if it has ever been used in non permeabilised cells, indicating that it can efficiently recognize an extracellular epitope.
   • We have done validation of this product in IHC-P of pancreas as well as spleen tissues after heat-induced antigen retrieval and yes, permeabilization was executed before blocking/primary antibody incubation step in the protocol. Have you seen our another GPR55 antibody (NLS6817)? This antibody has been generated employing an immunogen which was a synthetic peptide made to the 3rd extracellular domain of GPR55 protein. Because this antibody should technically be detecting extra-cellular domain of the target, you may try skipping permeabilization step for this antibody, however, you will still need to perform antigen retrieval.

Bioinformatics

• Gene Symbol: GPR55
• Entrez:
  • 9290(Human)
• Uniprot:
  • Q9Y2T6(Human)