| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85799-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-35333SS | |||
| Azide and BSA Free | NBP2-80622 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24449SS | |||
| Azide and BSA Free | NBP2-80930 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-189SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22423-0.025mg | |||
![Western Blot: CCR10/GPR2 Antibody [NB100-56319] - Analysis for GPR2/CCR10 using NB100-56319 at 2 ug/ml dilution against 10 ug of MCF-7 (a human breast cancer cell line) cell lysate.](http://images.novusbio.com/fullsize/CCR10-GPR2-Antibody-Western-Blot-NB100-56319-img0002.jpg "Western Blot: CCR10/GPR2 Antibody [NB100-56319] - Analysis for GPR2/CCR10 using NB100-56319 at 2 ug/ml dilution against 10 ug of MCF-7 (a human breast cancer cell line) cell lysate.")
![Simple Western: CCR10/GPR2 Antibody [NB100-56319] - Simple Western lane view shows a specific band for CCR10 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CCR10-GPR2-Antibody-Simple-Western-NB100-56319-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Rab13 Antibody [NBP1-85799] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Rab13-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85799-img0009.jpg)
![Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Rab13-Antibody-Immunohistochemistry-Paraffin-NBP1-85799-img0013.jpg)
![Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human stomach shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Rab13-Antibody-Immunohistochemistry-Paraffin-NBP1-85799-img0011.jpg)
![Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human prostate shows weak membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Rab13-Antibody-Immunohistochemistry-Paraffin-NBP1-85799-img0012.jpg)
![Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Rab13-Antibody-Immunohistochemistry-Paraffin-NBP1-85799-img0014.jpg)
![Western Blot: Rab13 Antibody [NBP1-85799] - RABIF distribution & effect on RAB13 expressionRepresentative immunofluorescence images of RABIF protein in cells transfected with the indicated siRNAs. Reduction of intensity in knockdown cells confirms the specificity of the signal. Calibration bar shows intensity values. Note that RABIF exhibits a mostly perinuclear enrichment.RAB13 expression in cells with CRISPR knockdown of RABIF using the indicated sgRNAs (see also Fig 7C). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-85799_rabbit-polyclonal-rab13-antibody-31020241539720.jpg)
![Western Blot: Rab13 Antibody [NBP1-85799] - RAB13 RNA mislocalization does not affect RAB13 binding to membranes or association with REP‐1 or RabGDICells treated with the indicated PMOs were fractionated into soluble & particulate fractions, & the indicated proteins were detected by Western blot. RhoGDI & TfRc serve as soluble & particulate markers, respectively.Lysates from the indicated GFP or GFP‐RAB13‐expressing cell lines were immunoprecipitated with anti‐GFP antibodies & blotted to detect the indicated proteins. Relative REP‐1 & RabGDI binding are quantified in the graphs from n = 3 (REP‐1) & n = 5 (RabGDI) independent experiments. Bars: mean ± s.e.m. No significant differences were detected by Wilcoxon matched‐pairs signed‐rank test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-85799_rabbit-polyclonal-rab13-antibody-310202415395940.jpg)
![Western Blot: Rab13 Antibody [NBP1-85799] - RAB13 RNA & protein exhibit distinct subcellular distributionsRepresentative FISH images showing RAB13 RNA distribution in MDA‐MB-231 cells. Nuclei & cell outlines are shown in blue & green, respectively. Arrows point to RAB13 RNA concentrated at protrusive regions. Boxed regions are magnified in the insets.Representative immunofluorescence images of RAB13 protein in cells transfected with the indicated siRNAs. Reduction of intensity in RAB13 knockdown cells confirms the specificity of the signal. Arrows point to perinuclear RAB13 protein. Calibration bar shows intensity values.Ratios of peripheral/perinuclear intensity calculated from images as shown in (A) & (B). Bars: mean ± s.e.m. Values within each bar represent number of cells observed in 3 independent experiments.Protrusions (Ps) & cell bodies (CB) of cells induced to migrate toward LPA were isolated & analyzed to detect the indicated proteins (by Western blot; left panels) or RNAs (by RT‐ddPCR; right panel). Ps/CB enrichment ratios from 2 independent experiments are shown. Bars: mean ± s.e.m. The enrichment of pY397‐FAK serves to verify the enrichment of protrusions containing newly formed adhesions in the Ps fraction.Data information: P‐values: **< 0.01; ****< 0.0001 by Student's t‐test (C) or analysis of variance with Dunnett's multiple comparisons test against GFP (D). Scale bars: 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-85799_rabbit-polyclonal-rab13-antibody-31020241612210.jpg)
![Western Blot: Rab13 Antibody [NBP1-85799] - Peripheral RAB13 RNA translation is required for RAB13 protein activation but not steady‐state distribution or membrane associationAWide‐field images of RAB13 immunofluorescence in MDA‐MB-231 cells treated with control or RAB13 (191 + 230) PMOs & ratios of peripheral/perinuclear intensity. Scale bars: 10 μm. n = 45–50 cells. Bars: mean ± s.e.m. Similar results were observed in two additional independent experiments.BFluorescence images (projections of confocal slices throughout the cell height) of cells expressing GFP‐RAB13 with the indicated UTRs. Note that in both cases the protein assumes indistinguishable distribution. Scale bars: 10 μm.CSoluble/particulate fractionation of the indicated cell lines followed by Western blot to detect the indicated proteins. RhoGDI & TfRc serve as soluble & particulate markers, respectively. Graph shows quantitation from n = 3 independent experiments. Bars: mean ± s.e.m.D, EActive RAB13 (RAB13‐GTP) was pulled down using MICAL‐L1 RBD‐GST from the indicated PMO‐treated cells (D) or GFP‐RAB13‐expressing lines (E). The amount of endogenous or exogenous RAB13 was measured by quantitative Western blot, & relative levels of active RAB13 are plotted. n = 8 (D), n = 4 (E). Bars: mean ± s.e.m.Data information: P‐values: *< 0.05, **< 0.01 by Kruskal–Wallis test.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-85799_rabbit-polyclonal-rab13-antibody-310202416121938.jpg)
![Immunocytochemistry/ Immunofluorescence: Rab13 Antibody [NBP1-85799] - RAB13 protein levels do not change upon serum stimulationMDA‐MB‐231 cells were stimulated with serum for the indicated times, in the presence or absence of cycloheximide (CHX). The cells were also treated with control or RAB13‐mislocalizing PMOs.Representative Western blot analysis of whole‐cell lysates & corresponding quantitations of RAB13 levels from n = 4–5 replicates. Bars: mean ± s.e.m. No significant differences by Friedman's test. Increase in pY397‐FAK levels attests to serum stimulation.RAB13 immunofluorescence at representative protrusive regions. A cell line expressing GFP was used to delineate cell borders & provide an internal cytosolic control. RAB13 signal at front lamellipodial regions was quantified. n = 35–51 protrusions. Bars: mean ± s.e.m. No increase is detected upon stimulation. By contrast, at early time points a decrease is detected (5 & 20 min, P < 0.01 by Kruskal–Wallis test), potentially arising from serum‐induced endocytosis of RAB13‐containing membranes. Scale bars: 8 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-85799_rabbit-polyclonal-rab13-antibody-310202416114715.jpg)
![Western Blot: Rab13 Antibody [NBP1-85799] - RAB13 protein levels do not change upon serum stimulationMDA‐MB‐231 cells were stimulated with serum for the indicated times, in the presence or absence of cycloheximide (CHX). The cells were also treated with control or RAB13‐mislocalizing PMOs.Representative Western blot analysis of whole‐cell lysates & corresponding quantitations of RAB13 levels from n = 4–5 replicates. Bars: mean ± s.e.m. No significant differences by Friedman's test. Increase in pY397‐FAK levels attests to serum stimulation.RAB13 immunofluorescence at representative protrusive regions. A cell line expressing GFP was used to delineate cell borders & provide an internal cytosolic control. RAB13 signal at front lamellipodial regions was quantified. n = 35–51 protrusions. Bars: mean ± s.e.m. No increase is detected upon stimulation. By contrast, at early time points a decrease is detected (5 & 20 min, P < 0.01 by Kruskal–Wallis test), potentially arising from serum‐induced endocytosis of RAB13‐containing membranes. Scale bars: 8 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-85799_rabbit-polyclonal-rab13-antibody-310202416121920.jpg)
![Western Blot: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female rats pretreated with V versus ER alpha or beta antagonist prior to intra-VMN V or NE infusion were analyzed by Western blot beta-1 Adrenergic R/ADRB1 protein expression. Nitrergic neuron beta 1-, F(5, 12)=11.72, p=.0003 protein profiles are depicted in Panels 3C; GABAergic neuron beta 1-, F(5, 12)=12.21, p=.0002 protein profiles are presented in Panels 3E. Data show mean normalized protein O.D. measures+/-SEMfor the following treatment groups: Veh/Veh (n=6), MPP/Veh (n=6), PHTPP/Veh (n=6), Veh/NE (n=6), MPP/NE (n=6), and PHTPP/NE (n=6). *p<.05; **p<.01; ***p<.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/beta-1 Adrenergic R-ADRB1 Antibody-Western Blot-NB600-978-img0004.jpg "Western Blot: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female rats pretreated with V versus ER alpha or beta antagonist prior to intra-VMN V or NE infusion were analyzed by Western blot beta-1 Adrenergic R/ADRB1 protein expression. Nitrergic neuron beta 1-, F(5, 12)=11.72, p=.0003 protein profiles are depicted in Panels 3C; GABAergic neuron beta 1-, F(5, 12)=12.21, p=.0002 protein profiles are presented in Panels 3E. Data show mean normalized protein O.D. measures+/-SEMfor the following treatment groups: Veh/Veh (n=6), MPP/Veh (n=6), PHTPP/Veh (n=6), Veh/NE (n=6), MPP/NE (n=6), and PHTPP/NE (n=6). *p<.05; **p<.01; ***p<.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/beta-1-Adrenergic-R-ADRB1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-978-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.](http://images.novusbio.com/fullsize/beta-1-Adrenergic-R-ADRB1-Antibody-Immunohistochemistry-Paraffin-NB600-978-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Staining of paraffin embedded Human Heart with antibody at 6 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/beta-1-Adrenergic-R-ADRB1-Antibody-Immunohistochemistry-Paraffin-NB600-978-img0001.jpg)
![Western Blot: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Effects of MPP Versus PHTPP on NE Regulation of VMN Nitrergic & GABA Neuron Adrenergic Receptor Protein Expression. Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female rats pretreated with V versus ER alpha or -beta antagonist prior to intra-VMN V or NE infusion were analyzed by Western blot for alpha1- ( alpha 1-), alpha2- ( alpha 2-), or beta1- ( beta 1-) AR protein expression. Nitrergic neuron alpha 1-, F(5, 12) = 10.51, p = .0005; alpha 2-, F(5, 12) = 16.50, p < .0001; & beta 1-, F(5, 12) = 11.72, p = .0003 protein profiles are depicted in Panels 3A to C; GABAergic neuron alpha 1-, F(5, 12) = 5.52, p = .007; alpha 2-, F(5, 12) = 10.47, p < .0001; & beta 1-, F(5, 12) = 12.21, p = .0002 protein profiles are presented in Panels 3D to F. Data show mean normalized protein O.D. measures ± SEM for the following treatment groups: Veh/Veh (solid white bars, n = 6), MPP/Veh (diagonal-striped white bars, n = 6), PHTPP/Veh (cross-hatched white bars, n = 6), Veh/NE (solid gray bars, n = 6), MPP/NE (diagonal-striped gray bars, n = 6), & PHTPP/NE (cross-hatched gray bars, n = 6). *p < .05; **p < .01; ***p < .001. alpha 1-AR = alpha1 adrenergic receptor; alpha 2-AR = alpha2 adrenergic receptor; beta 1-AR = beta1 adrenergic receptor; MPP = 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride; PHTPP = 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol; NE = norepinephrine. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-978_goat-polyclonal-beta-1-adrenergic-r-adrb1-antibody-310202416163766.jpg)
![Western Blot: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Effects of MPP Versus PHTPP on NE Regulation of VMN Nitrergic & GABA Neuron Adrenergic Receptor Protein Expression. Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female rats pretreated with V versus ER alpha or -beta antagonist prior to intra-VMN V or NE infusion were analyzed by Western blot for alpha1- ( alpha 1-), alpha2- ( alpha 2-), or beta1- ( beta 1-) AR protein expression. Nitrergic neuron alpha 1-, F(5, 12) = 10.51, p = .0005; alpha 2-, F(5, 12) = 16.50, p < .0001; & beta 1-, F(5, 12) = 11.72, p = .0003 protein profiles are depicted in Panels 3A to C; GABAergic neuron alpha 1-, F(5, 12) = 5.52, p = .007; alpha 2-, F(5, 12) = 10.47, p < .0001; & beta 1-, F(5, 12) = 12.21, p = .0002 protein profiles are presented in Panels 3D to F. Data show mean normalized protein O.D. measures ± SEM for the following treatment groups: Veh/Veh (solid white bars, n = 6), MPP/Veh (diagonal-striped white bars, n = 6), PHTPP/Veh (cross-hatched white bars, n = 6), Veh/NE (solid gray bars, n = 6), MPP/NE (diagonal-striped gray bars, n = 6), & PHTPP/NE (cross-hatched gray bars, n = 6). *p < .05; **p < .01; ***p < .001. alpha 1-AR = alpha1 adrenergic receptor; alpha 2-AR = alpha2 adrenergic receptor; beta 1-AR = beta1 adrenergic receptor; MPP = 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride; PHTPP = 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol; NE = norepinephrine. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-978_goat-polyclonal-beta-1-adrenergic-r-adrb1-antibody-31020241616042.jpg)
![ELISA: Relaxin R1/LGR7 Antibody (3E3) [H00059350-M01] - Detection limit for recombinant GST tagged LGR7 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Relaxin-R1-LGR7-Antibody-3E3-ELISA-H00059350-M01-img0003.jpg "ELISA: Relaxin R1/LGR7 Antibody (3E3) [H00059350-M01] - Detection limit for recombinant GST tagged LGR7 is approximately 3ng/ml as a capture antibody.")
![Western Blot: CCR2 Antibody (2D8) [NBP2-35333] - Analysis of CCR2 [2D8] in Molt4 lysate](http://images.novusbio.com/fullsize/CCR2-Antibody-2D8-Western-Blot-NBP2-35333-img0003.jpg "Western Blot: CCR2 Antibody (2D8) [NBP2-35333] - Analysis of CCR2 [2D8] in Molt4 lysate")
![Immunohistochemistry-Paraffin: CCR2 Antibody (2D8) [NBP2-35333] - Analysis of FFPE tissue section of human esophageal squamous cell carcinoma (SCC) using CCR2 antibody (clone 2D8) at 5 ug/ml concentration. The cancer cells depicted strong cytoplasmic-membranous immunostaining, whereas the cells of tumor stroma were largely negative for CCR2 immunopositivity.](http://images.novusbio.com/fullsize/CCR2-Antibody-2D8-Immunohistochemistry-Paraffin-NBP2-35333-img0002.jpg)
![Immunohistochemistry-Paraffin: CCR2 Antibody (2D8) [NBP2-35333] - Analysis of FFPE tissue section of human breast using CCR2 antibody (clone 2D8) at 5 ug/ml concentration. The glandular cells in the lobules showed a specific cytoplasmic-membranous immunostaining, whereas, the inter-lobular connective tissue did not develop any staining for CCR2.](http://images.novusbio.com/fullsize/CCR2-Antibody-2D8-Immunohistochemistry-Paraffin-NBP2-35333-img0001.jpg)
![Immunohistochemistry-Paraffin: Complement Component C3aR Antibody [NBP2-15649] - Complement Component C3a R Antibody [NBP2-15649] - Tissue staining sections of mouse lung tissue without (left) and with (right) silencing by siRNA .Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Complement-Component-C3a-R-Antibody-Immunohistochemistry-Paraffin-NBP2-15649-img0007.jpg "<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Complement Component C3aR Antibody [NBP2-15649] - Complement Component C3a R Antibody [NBP2-15649] - Tissue staining sections of mouse lung tissue without (left) and with (right) silencing by siRNA .Image submitted by a verified customer review.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Complement Component C3a R Antibody [NBP2-15649] - Human colon carcinoma, using C3a receptor 1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Complement-Component-C3a-R-Antibody-Immunohistochemistry-Paraffin-NBP2-15649-img0003.jpg)
![Western Blot: Complement Component C3aR Antibody [NBP2-15649] - Boiled HL-60 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Complement Component C3aR antibody (NBP2-15649) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15649_rabbit-complement-component-c3ar-pab-910202420131262.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Complement Component C3aR Antibody [NBP2-15649] - Non-transfected (–) and transfected (+) unboiled HL-60 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C3a Receptor antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15649_rabbit-complement-component-c3ar-pab-1610202419405768.jpg)
![Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CXCR7-RDC-1-Antibody-Western-Blot-NBP1-31309-img0012.jpg "Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: CXCR7/RDC-1 Antibody [NBP1-31309] - Analysis of human pancreatic cancer cell line, using CCR7 antibody at 1:50 dilution. Blue: DAPI.](http://images.novusbio.com/fullsize/CXCR7-RDC-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31309-img0007.jpg)
![Immunohistochemistry-Paraffin: CXCR7/RDC-1 Antibody [NBP1-31309] - Human gastric cancer, using CXCR7 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CXCR7-RDC-1-Antibody-Immunohistochemistry-Paraffin-NBP1-31309-img0010.jpg)
![Immunohistochemistry: CXCR7/RDC-1 Antibody [NBP1-31309] - Human pancreatic tumor, using CXCR7 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CXCR7-RDC-1-Antibody-Immunohistochemistry-NBP1-31309-img0008.jpg)
![Immunohistochemistry: CXCR7/RDC-1 Antibody [NBP1-31309] - Human pancreatic tumor, using CXCR7 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CXCR7-RDC-1-Antibody-Immunohistochemistry-NBP1-31309-img0009.jpg)
![Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] - Unboiled Raji whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-31309_rabbit-polyclonal-cxcr7-rdc-1-antibody-western-blot-12120249813..jpg)
![Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-31309_rabbit-polyclonal-cxcr7-rdc-1-antibody-western-blot-12120249944..jpg)
![Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-31309_rabbit-polyclonal-cxcr7-rdc-1-antibody-western-blot-12120249115..jpg)
![Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-31309_rabbit-polyclonal-cxcr7-rdc-1-antibody-western-blot-121202491219..jpg)
![Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -Boiled and unboiled Raji whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)](http://images.novusbio.com/fullsize/antibody/nbp1-31309_rabbit-polyclonal-cxcr7-rdc-1-antibody-western-blot-121202491348..jpg)
![Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] - Unboiled Raji whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7/RDC-1 antibody [C1C2], Internal (NBP1-31309) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)](http://images.novusbio.com/fullsize/nbp1-31309_rabbit-polyclonal-cxcr7-rdc-1-antibody-9102024205633.jpg)
![Immunohistochemistry-Paraffin: Relaxin R2/LGR8 Antibody [NLS4753] - Brain, Glioblastoma](http://images.novusbio.com/fullsize/Relaxin-R2-LGR8-Antibody-Immunohistochemistry-Paraffin-NLS4753-img0005.jpg "Immunohistochemistry-Paraffin: Relaxin R2/LGR8 Antibody [NLS4753] - Brain, Glioblastoma")
![Immunohistochemistry-Paraffin: Relaxin R2/LGR8 Antibody [NLS4753] - Human Uterus](http://images.novusbio.com/fullsize/Relaxin-R2-LGR8-Antibody-Immunohistochemistry-Paraffin-NLS4753-img0001.jpg)
![Immunohistochemistry-Paraffin: Relaxin R2/LGR8 Antibody [NLS4753] - Skin, Malignant Melanoma](http://images.novusbio.com/fullsize/Relaxin-R2-LGR8-Antibody-Immunohistochemistry-Paraffin-NLS4753-img0002.jpg)
![Immunohistochemistry-Paraffin: Relaxin R2/LGR8 Antibody [NLS4753] - Lung, Non Small-Cell Carcinoma](http://images.novusbio.com/fullsize/Relaxin-R2-LGR8-Antibody-Immunohistochemistry-Paraffin-NLS4753-img0003.jpg)
![Immunohistochemistry-Paraffin: Relaxin R2/LGR8 Antibody [NLS4753] - Breast, carcinoma](http://images.novusbio.com/fullsize/Relaxin-R2-LGR8-Antibody-Immunohistochemistry-Paraffin-NLS4753-img0004.jpg)
![Western Blot: ROR gamma/RORC/NR1F3 Antibody (4G419) [NBP2-24449] - Analysis in (A) human thymus lysate, (B) transfected 293 cell lysate, and (C) untransfected 293 cell lysate. Dilution: 5 ug/ml.](http://images.novusbio.com/fullsize/ROR-gamma-RORC-NR1F3-Antibody-4G419-Western-Blot-NBP2-24449-img0001.jpg "Western Blot: ROR gamma/RORC/NR1F3 Antibody (4G419) [NBP2-24449] - Analysis in (A) human thymus lysate, (B) transfected 293 cell lysate, and (C) untransfected 293 cell lysate. Dilution: 5 ug/ml.")
![Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) [NBP2-24449] - Analysis using the FITC conjugate of NBP2-24449. Staining of ROR gamma in human lymphocytes using 10 ul (0.25 ug) of ROR gamma antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody; red represents ROR gamma](http://images.novusbio.com/fullsize/ROR-gamma-RORC-NR1F3-Antibody-4G419-Flow-Cytometry-NBP2-24449-img0007.jpg)
![Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) [NBP2-24449] - Intracellular analysis of ROR gamma in human lymphocytes using 0.25 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody ; red represents ROR gamma antibody.](http://images.novusbio.com/fullsize/ROR-gamma-RORC-NR1F3-Antibody-4G419-Flow-Cytometry-NBP2-24449-img0002.jpg)
![Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) [NBP2-24449] - Intracellular flow cytometric analysis of ROR gamma in human monocytes using 0.25 ug of ROR gamma antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody (Novus, 20109); red represents ROR gamma antibody.](http://images.novusbio.com/fullsize/ROR-gamma-RORC-NR1F3-Antibody-4G419-Flow-Cytometry-NBP2-24449-img0003.jpg)
![Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) [NBP2-24449] - Analysis using the Allophycocyanin conjugate of NBP2-24449. Staining of ROR gamma in human lymphocytes using 10 ul (0.25 ug) of ROR gamma antibody. Shaded histogram represents cells without antibody; green represents isotype control antibodywas used for this test.](http://images.novusbio.com/fullsize/ROR-gamma-RORC-NR1F3-Antibody-4G419-Flow-Cytometry-NBP2-24449-img0006.jpg)
![Western Blot: metabotropic Glutamate Receptor 6 Antibody [NB300-189] - Detection of metabotropic Glutamate Receptor 6 in mouse brain lysate at 0.5 ug/ml.](http://images.novusbio.com/fullsize/metabotropic-Glutamate-Receptor-6-Antibody-Western-Blot-NB300-189-img0006.jpg "Western Blot: metabotropic Glutamate Receptor 6 Antibody [NB300-189] - Detection of metabotropic Glutamate Receptor 6 in mouse brain lysate at 0.5 ug/ml.")
![Immunocytochemistry/Immunofluorescence: mGluR6 Antibody [NB300-189] - metabotropic Glutamate Receptor 6 Antibody [NB300-189] - Detection of metabotropic Glutamate Receptor 6 in trapezoid body in mouse brain using NB300-189 and anti-rabbit Cy3 conjugated secondary antibodies (red). DAPI was used to counterstain cell nuclei.](http://images.novusbio.com/fullsize/metabotropic-Glutamate-Receptor-6-Antibody-Immunocytochemistry-Immunofluorescence-NB300-189-img0005.jpg)
![Western Blot: GIT1 Antibody (S39B-8) [NBP2-22423] - Western Blot analysis of Rat brain membrane lysate showing detection of GIT1 protein using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (NBP2-22423). Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (NBP2-22423) at 1:1000.](http://images.novusbio.com/fullsize/GIT1-Antibody-S39B-8-Western-Blot-NBP2-22423-img0003.jpg "Western Blot: GIT1 Antibody (S39B-8) [NBP2-22423] - Western Blot analysis of Rat brain membrane lysate showing detection of GIT1 protein using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (NBP2-22423). Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (NBP2-22423) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (NBP2-22423). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (NBP2-22423) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GIT1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GIT1-Antibody-S39B-8-Immunocytochemistry-Immunofluorescence-NBP2-22423-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm . Magnification: 60X.](http://images.novusbio.com/fullsize/GIT1-Antibody-S39B-8-Immunocytochemistry-Immunofluorescence-NBP2-22423-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (NBP2-22423). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (NBP2-22423) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GIT1 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/GIT1-Antibody-S39B-8-Immunocytochemistry-Immunofluorescence-NBP2-22423-img0005.jpg)
![Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Gene and protein expression of components of the PAR-1/SphK/S1P axis. Western blot detection and quantification of the protein expression of PAR-1, S1PR1, SphK1, and SphK2 in astrocytes treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only), for 6 h. The data are presented as the mean +/- SD (n = 3), *p < 0.05. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/article/10.3389/fnmol.2020.00114/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-Western-Blot-NB120-11424-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Gene and protein expression of components of the PAR-1/SphK/S1P axis. Western blot detection and quantification of the protein expression of PAR-1, S1PR1, SphK1, and SphK2 in astrocytes treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only), for 6 h. The data are presented as the mean +/- SD (n = 3), *p < 0.05. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/article/10.3389/fnmol.2020.00114/full) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody [NB120-11424] - Immunofluorescence of PAR-1 and S1PR1 in spinal cord tissues of mice induced by EAE. Mice subjected to EAE modeling were treated with vehicle (physiological saline), Dab, or PAR-1-inh. Tissues were stained for (B) S1PR1 in green and nuclei (DAPI) in blue. Scale bar = 100 um. Green fluorescence was quantified by measuring the relative mean IOD. The data are presented as the mean +/- SD (n = 6), *p < 0.05. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/articles/10.3389/fnmol.2020.00114/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11424-img0008.jpg)
![Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Analysis of S1P1 from transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-Western-Blot-NB120-11424-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody [NB120-11424] - Immunofluorescence staining of S1P1 in transfected CCL39 cells using NB120-11424.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11424-img0004.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody [NB120-11424] - Immunofluorescence of proteins related to S1P1/EDG-1 signaling in astrocytes. Astrocytes were treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only) for 6 h. Cells were stained for S1P1/EDG-1 receptor 1 in green and nuclei (DAPI) in blue. Scale bar = 100 um. Green fluorescence was quantified by measuring the relative mean IOD. The data are presented as the mean +/- SD (n = 3), *p < 0.05. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/article/10.3389/fnmol.2020.00114/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11424-img0009.jpg)
![Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Gene & protein expression of components of the PAR-1/SphK/S1P axis. qRT-PCR analysis of the gene expression of (A) PAR-1, (B) S1PR1, (C) SphK1, & (D) SphK2 in astrocytes treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only), for 0 h, 1 h, or 6 h. Western blot detection & quantification of the protein expression of PAR-1, S1PR1, SphK1, & SphK2 in astrocytes treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only), for (E) 0 h, (F) 1 h, or (G) 6 h. The data are presented as the mean ± SD (n = 3), *p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32694981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-11424_rabbit-polyclonal-s1p1-edg-1-antibody-310202415175259.jpg)
![Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Expression of sphingosine-1-phosphate (S1P), interleukin (IL)-1 beta , & protease-activated receptor-1 (PAR-1) in isolated astrocytes. (A) Isolated & purified astrocytes were identified by bright-field microscopy & immunofluorescence for glial fibrillary acid protein (GFAP; red). Nuclei (DAPI) were labeled in blue. Scale bar = 100 μm. (B) Western blot & quantitative reverse transcription polymerase chain reaction (qRT-PCR) of the protein & mRNA expression, respectively, of S1P 0, 1, & 6 h after astrocytes were treated with lipopolysaccharide (LPS) or thrombin & Dabigatran (Dab) or PAR-1-inh (LPS only). (D) qRT-PCR & (E) enzyme-linked immunosorbent assay (ELISA) of the mRNA expression & secretion, respectively, of IL-1 beta 0, 1, & 6 h after astrocytes were treated with LPS or thrombin & Dab or PAR-1-inh (LPS only). (F) Immunofluorescence of astrocytes & quantification of relative mean integrated optical density (IOD; green fluorescence). PAR-1 was stained in green & nuclei (DAPI) were stained in blue. Scale bar = 100 μm. For (B–F), data are presented as the mean ± SD (n = 3). *p < 0.05; #p < 0.05 vs. the same group at 0 h; &p < 0.05 vs. the same group at 1 h. ns, not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32694981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-11424_rabbit-polyclonal-s1p1-edg-1-antibody-310202416205112.jpg)