GPCR reagents: Novus Biologicals

NBP1-85799

Rab13 Antibody - BSA Free

(5 Publications)

0.1 ml

NBP1-85799

Immunocytochemistry/Immunofluorescence: Rab13 Antibody [NBP1-85799] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human stomach shows moderate membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human prostate shows weak membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: Rab13 Antibody [NBP1-85799] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Western Blot: Rab13 Antibody [NBP1-85799] - RABIF distribution & effect on RAB13 expressionRepresentative immunofluorescence images of RABIF protein in cells transfected with the indicated siRNAs. Reduction of intensity in knockdown cells confirms the specificity of the signal. Calibration bar shows intensity values. Note that RABIF exhibits a mostly perinuclear enrichment.RAB13 expression in cells with CRISPR knockdown of RABIF using the indicated sgRNAs (see also Fig 7C). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: Rab13 Antibody [NBP1-85799] - RAB13 RNA mislocalization does not affect RAB13 binding to membranes or association with REP‐1 or RabGDICells treated with the indicated PMOs were fractionated into soluble & particulate fractions, & the indicated proteins were detected by Western blot. RhoGDI & TfRc serve as soluble & particulate markers, respectively.Lysates from the indicated GFP or GFP‐RAB13‐expressing cell lines were immunoprecipitated with anti‐GFP antibodies & blotted to detect the indicated proteins. Relative REP‐1 & RabGDI binding are quantified in the graphs from n = 3 (REP‐1) & n = 5 (RabGDI) independent experiments. Bars: mean ± s.e.m. No significant differences were detected by Wilcoxon matched‐pairs signed‐rank test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Western Blot: Rab13 Antibody [NBP1-85799] - RAB13 RNA & protein exhibit distinct subcellular distributionsRepresentative FISH images showing RAB13 RNA distribution in MDA‐MB-231 cells. Nuclei & cell outlines are shown in blue & green, respectively. Arrows point to RAB13 RNA concentrated at protrusive regions. Boxed regions are magnified in the insets.Representative immunofluorescence images of RAB13 protein in cells transfected with the indicated siRNAs. Reduction of intensity in RAB13 knockdown cells confirms the specificity of the signal. Arrows point to perinuclear RAB13 protein. Calibration bar shows intensity values.Ratios of peripheral/perinuclear intensity calculated from images as shown in (A) & (B). Bars: mean ± s.e.m. Values within each bar represent number of cells observed in 3 independent experiments.Protrusions (Ps) & cell bodies (CB) of cells induced to migrate toward LPA were isolated & analyzed to detect the indicated proteins (by Western blot; left panels) or RNAs (by RT‐ddPCR; right panel). Ps/CB enrichment ratios from 2 independent experiments are shown. Bars: mean ± s.e.m. The enrichment of pY397‐FAK serves to verify the enrichment of protrusions containing newly formed adhesions in the Ps fraction.Data information: P‐values: **< 0.01; ****< 0.0001 by Student's t‐test (C) or analysis of variance with Dunnett's multiple comparisons test against GFP (D). Scale bars: 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Western Blot: Rab13 Antibody [NBP1-85799] - Peripheral RAB13 RNA translation is required for RAB13 protein activation but not steady‐state distribution or membrane associationAWide‐field images of RAB13 immunofluorescence in MDA‐MB-231 cells treated with control or RAB13 (191 + 230) PMOs & ratios of peripheral/perinuclear intensity. Scale bars: 10 μm. n = 45–50 cells. Bars: mean ± s.e.m. Similar results were observed in two additional independent experiments.BFluorescence images (projections of confocal slices throughout the cell height) of cells expressing GFP‐RAB13 with the indicated UTRs. Note that in both cases the protein assumes indistinguishable distribution. Scale bars: 10 μm.CSoluble/particulate fractionation of the indicated cell lines followed by Western blot to detect the indicated proteins. RhoGDI & TfRc serve as soluble & particulate markers, respectively. Graph shows quantitation from n = 3 independent experiments. Bars: mean ± s.e.m.D, EActive RAB13 (RAB13‐GTP) was pulled down using MICAL‐L1 RBD‐GST from the indicated PMO‐treated cells (D) or GFP‐RAB13‐expressing lines (E). The amount of endogenous or exogenous RAB13 was measured by quantitative Western blot, & relative levels of active RAB13 are plotted. n = 8 (D), n = 4 (E). Bars: mean ± s.e.m.Data information: P‐values: *< 0.05, **< 0.01 by Kruskal–Wallis test.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Immunocytochemistry/ Immunofluorescence: Rab13 Antibody [NBP1-85799] - RAB13 protein levels do not change upon serum stimulationMDA‐MB‐231 cells were stimulated with serum for the indicated times, in the presence or absence of cycloheximide (CHX). The cells were also treated with control or RAB13‐mislocalizing PMOs.Representative Western blot analysis of whole‐cell lysates & corresponding quantitations of RAB13 levels from n = 4–5 replicates. Bars: mean ± s.e.m. No significant differences by Friedman's test. Increase in pY397‐FAK levels attests to serum stimulation.RAB13 immunofluorescence at representative protrusive regions. A cell line expressing GFP was used to delineate cell borders & provide an internal cytosolic control. RAB13 signal at front lamellipodial regions was quantified. n = 35–51 protrusions. Bars: mean ± s.e.m. No increase is detected upon stimulation. By contrast, at early time points a decrease is detected (5 & 20 min, P < 0.01 by Kruskal–Wallis test), potentially arising from serum‐induced endocytosis of RAB13‐containing membranes. Scale bars: 8 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Rab13 Antibody [NBP1-85799] - RAB13 protein levels do not change upon serum stimulationMDA‐MB‐231 cells were stimulated with serum for the indicated times, in the presence or absence of cycloheximide (CHX). The cells were also treated with control or RAB13‐mislocalizing PMOs.Representative Western blot analysis of whole‐cell lysates & corresponding quantitations of RAB13 levels from n = 4–5 replicates. Bars: mean ± s.e.m. No significant differences by Friedman's test. Increase in pY397‐FAK levels attests to serum stimulation.RAB13 immunofluorescence at representative protrusive regions. A cell line expressing GFP was used to delineate cell borders & provide an internal cytosolic control. RAB13 signal at front lamellipodial regions was quantified. n = 35–51 protrusions. Bars: mean ± s.e.m. No increase is detected upon stimulation. By contrast, at early time points a decrease is detected (5 & 20 min, P < 0.01 by Kruskal–Wallis test), potentially arising from serum‐induced endocytosis of RAB13‐containing membranes. Scale bars: 8 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32946136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

<b>Genetic Strategies Validation. </b>Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RAB13 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-85799-25ul

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NB100-56319

CCR10/GPR2 Antibody

(7 Publications)

0.1 mg

NB100-56319

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-286

Alt Catalog# IMG-286

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP1-31309

CXCR7/RDC-1 Antibody

(3 Publications)

100 ul

NBP1-31309

Immunohistochemistry-Paraffin: CXCR7/RDC-1 Antibody [NBP1-31309] - Human gastric cancer, using CXCR7 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry: CXCR7/RDC-1 Antibody [NBP1-31309] - Human pancreatic tumor, using CXCR7 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] - Unboiled Raji whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody.

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -Boiled and unboiled Raji whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)

CXCR7/RDC-1 antibody [C1C2], Internal detects CXCR7/RDC-1 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CXCR7/RDC-1 stained by CXCR7/RDC-1 antibody [C1C2], Internal (NBP1-31309) diluted at 1:500.Blue: Fluoroshield with DAPI .

Reactivity:

Hu, Mu, Rt, Ca, RM

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, RM

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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H00059350-M01

Relaxin R1/LGR7 Antibody (3E3) - Azide and BSA Free

(11 Publications)

0.1 mg

H00059350-M01

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NB120-11424

S1P1/EDG-1 Antibody

(5 Publications)

100 μg

NB120-11424

Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Analysis of S1P1 from transfected HEK293 cell lysate.

Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody [NB120-11424] - Immunofluorescence staining of S1P1 in transfected CCL39 cells using NB120-11424.

<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody [NB120-11424] - Immunofluorescence of proteins related to S1P1/EDG-1 signaling in astrocytes. Astrocytes were treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only) for 6 h. Cells were stained for S1P1/EDG-1 receptor 1 in green and nuclei (DAPI) in blue. Scale bar = 100 um. Green fluorescence was quantified by measuring the relative mean IOD. The data are presented as the mean +/- SD (n = 3), *p < 0.05. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/article/10.3389/fnmol.2020.00114/full) licensed under a CC-BY license.

Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Gene & protein expression of components of the PAR-1/SphK/S1P axis. qRT-PCR analysis of the gene expression of (A) PAR-1, (B) S1PR1, (C) SphK1, & (D) SphK2 in astrocytes treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only), for 0 h, 1 h, or 6 h. Western blot detection & quantification of the protein expression of PAR-1, S1PR1, SphK1, & SphK2 in astrocytes treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only), for (E) 0 h, (F) 1 h, or (G) 6 h. The data are presented as the mean ± SD (n = 3), *p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32694981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Expression of sphingosine-1-phosphate (S1P), interleukin (IL)-1 beta , & protease-activated receptor-1 (PAR-1) in isolated astrocytes. (A) Isolated & purified astrocytes were identified by bright-field microscopy & immunofluorescence for glial fibrillary acid protein (GFAP; red). Nuclei (DAPI) were labeled in blue. Scale bar = 100 μm. (B) Western blot & quantitative reverse transcription polymerase chain reaction (qRT-PCR) of the protein & mRNA expression, respectively, of S1P 0, 1, & 6 h after astrocytes were treated with lipopolysaccharide (LPS) or thrombin & Dabigatran (Dab) or PAR-1-inh (LPS only). (D) qRT-PCR & (E) enzyme-linked immunosorbent assay (ELISA) of the mRNA expression & secretion, respectively, of IL-1 beta 0, 1, & 6 h after astrocytes were treated with LPS or thrombin & Dab or PAR-1-inh (LPS only). (F) Immunofluorescence of astrocytes & quantification of relative mean integrated optical density (IOD; green fluorescence). PAR-1 was stained in green & nuclei (DAPI) were stained in blue. Scale bar = 100 μm. For (B–F), data are presented as the mean ± SD (n = 3). *p < 0.05; #p < 0.05 vs. the same group at 0 h; &p < 0.05 vs. the same group at 1 h. ns, not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32694981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB600-978

beta-1 Adrenergic R/ADRB1 Antibody

(7 Publications)

0.1 mg

NB600-978

Immunohistochemistry-Paraffin: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.

Immunohistochemistry-Paraffin: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Staining of paraffin embedded Human Heart with antibody at 6 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Western Blot: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Effects of MPP Versus PHTPP on NE Regulation of VMN Nitrergic & GABA Neuron Adrenergic Receptor Protein Expression. Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female rats pretreated with V versus ER alpha or -beta antagonist prior to intra-VMN V or NE infusion were analyzed by Western blot for alpha1- ( alpha 1-), alpha2- ( alpha 2-), or beta1- ( beta 1-) AR protein expression. Nitrergic neuron alpha 1-, F(5, 12) = 10.51, p = .0005; alpha 2-, F(5, 12) = 16.50, p < .0001; & beta 1-, F(5, 12) = 11.72, p = .0003 protein profiles are depicted in Panels 3A to C; GABAergic neuron alpha 1-, F(5, 12) = 5.52, p = .007; alpha 2-, F(5, 12) = 10.47, p < .0001; & beta 1-, F(5, 12) = 12.21, p = .0002 protein profiles are presented in Panels 3D to F. Data show mean normalized protein O.D. measures ± SEM for the following treatment groups: Veh/Veh (solid white bars, n = 6), MPP/Veh (diagonal-striped white bars, n = 6), PHTPP/Veh (cross-hatched white bars, n = 6), Veh/NE (solid gray bars, n = 6), MPP/NE (diagonal-striped gray bars, n = 6), & PHTPP/NE (cross-hatched gray bars, n = 6). *p < .05; **p < .01; ***p < .001. alpha 1-AR = alpha1 adrenergic receptor; alpha 2-AR = alpha2 adrenergic receptor; beta 1-AR = beta1 adrenergic receptor; MPP = 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride; PHTPP = 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol; NE = norepinephrine. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Ca

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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NLS4753

Relaxin R2/LGR8 Antibody - BSA Free

(1 Review)

(3 Publications)

0.05 ml

NLS4753

Immunohistochemistry-Paraffin: Relaxin R2/LGR8 Antibody [NLS4753] - Skin, Malignant Melanoma

Immunohistochemistry-Paraffin: Relaxin R2/LGR8 Antibody [NLS4753] - Lung, Non Small-Cell Carcinoma

Immunohistochemistry-Paraffin: Relaxin R2/LGR8 Antibody [NLS4753] - Breast, carcinoma

Reactivity:

Hu, Mu, Rt, Dr, Eq, Pm, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Dr, Eq, Pm, Pm

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-24449

ROR gamma/RORC/NR1F3 Antibody (4G419) - BSA Free

(5 Publications)

0.1 mg

NBP2-24449

Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) [NBP2-24449] - Intracellular analysis of ROR gamma in human lymphocytes using 0.25 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody ; red represents ROR gamma antibody.

Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) [NBP2-24449] - Intracellular flow cytometric analysis of ROR gamma in human monocytes using 0.25 ug of ROR gamma antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody (Novus, 20109); red represents ROR gamma antibody.

Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) [NBP2-24449] - Analysis using the Allophycocyanin conjugate of NBP2-24449. Staining of ROR gamma in human lymphocytes using 10 ul (0.25 ug) of ROR gamma antibody. Shaded histogram represents cells without antibody; green represents isotype control antibodywas used for this test.

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-6275A

Alt Catalog# IMG-6275A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-24449AF350
Alexa Fluor 405	NBP2-24449AF405
Alexa Fluor 488	NBP2-24449AF488
Alexa Fluor 532	NBP2-24449AF532
Alexa Fluor 594	NBP2-24449AF594
Alexa Fluor 647	NBP2-24449AF647
Alexa Fluor 700	NBP2-24449AF700
Alexa Fluor 750	NBP2-24449AF750
Allophycocyanin	NBP2-24449APC
Allophycocyanin	NBP2-24451
Biotin	NBP2-24449B
CoraFluor 1	NBP2-24449CL1
DyLight 350	NBP2-24449UV
DyLight 405	NBP2-24449V
DyLight 488	NBP2-24449G
DyLight 550	NBP2-24449R
DyLight 594	NBP2-24449DL594
DyLight 650	NBP2-24449C
DyLight 680	NBP2-24449FR
DyLight 755	NBP2-24449IR
FITC	NBP2-24447
FITC	NBP2-24449F
HRP	NBP2-24449H
Janelia Fluor 525	NBP2-24449JF525
Janelia Fluor 549	NBP2-24449JF549
Janelia Fluor 646	NBP2-24449JF646
Janelia Fluor 669	NBP2-24449JF669
PE	NBP2-24452
PE	NBP2-24449PE
PerCP	NBP2-24449PCP
mFluor Violet 450 SE	NBP2-24449MFV450
mFluor Violet 500 SE	NBP2-24449MFV500
mFluor Violet 610 SE	NBP2-24449MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-24449SS
Azide and BSA Free	NBP2-80930

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NB300-189

mGluR6 Antibody - BSA Free

(6 Publications)

0.1 ml

NB300-189

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB300-189AF350
Alexa Fluor 405	NB300-189AF405
Alexa Fluor 488	NB300-189AF488
Alexa Fluor 532	NB300-189AF532
Alexa Fluor 594	NB300-189AF594
Alexa Fluor 647	NB300-189AF647
Alexa Fluor 700	NB300-189AF700
Alexa Fluor 750	NB300-189AF750
Allophycocyanin	NB300-189APC
Biotin	NB300-189B
CoraFluor 1	NB300-189CL1
DyLight 350	NB300-189UV
DyLight 405	NB300-189V
DyLight 488	NB300-189G
DyLight 550	NB300-189R
DyLight 594	NB300-189DL594
DyLight 650	NB300-189C
DyLight 680	NB300-189FR
DyLight 755	NB300-189IR
FITC	NB300-189F
HRP	NB300-189H
Janelia Fluor 525	NB300-189JF525
Janelia Fluor 549	NB300-189JF549
Janelia Fluor 646	NB300-189JF646
Janelia Fluor 669	NB300-189JF669
PE	NB300-189PE
PerCP	NB300-189PCP
mFluor Violet 450 SE	NB300-189MFV450
mFluor Violet 500 SE	NB300-189MFV500
mFluor Violet 610 SE	NB300-189MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB300-189SS

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NBP2-15649

Complement Component C3aR Antibody - BSA Free

(1 Review)

(4 Publications)

0.1 ml

NBP2-15649

<b>Genetic Strategies Validation. </b>Western Blot: Complement Component C3aR Antibody [NBP2-15649] - Non-transfected (–) and transfected (+) unboiled HL-60 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C3a Receptor antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-84576

Integrin alpha 5/CD49e Antibody - BSA Free

(1 Review)

(2 Publications)

0.1 ml

NBP1-84576

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human placenta shows high expression.

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human skeletal muscle shows low expression as expected.

Immunohistochemistry-Paraffin: Integrin alpha 5/CD49e Antibody [NBP1-84576] - Staining of human prostate shows moderate membranous positivity in smooth muscle cells.

<b>Orthogonal Strategies Validation. </b>Analysis in human cell lines U2OS and SK-MEL-30 using Anti-ITGA5 antibody. Corresponding ITGA5 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-84576-25ul

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NB100-65530

SIRP alpha/CD172a Antibody (OX-41) - BSA Free

(5 Publications)

250 μg

NB100-65530

Immunofluorescence: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, red in A and Mouse anti Rat CD4, green in B. C is the Merged image with nuclei counter-stained blue using DAPI. Low power

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-65530AF350
Alexa Fluor 405	NB100-65530AF405
Alexa Fluor 488	NB100-65530AF488
Alexa Fluor 532	NB100-65530AF532
Alexa Fluor 594	NB100-65530AF594
Alexa Fluor 647	NB100-65530AF647
Alexa Fluor 700	NB100-65530AF700
Alexa Fluor 750	NB100-65530AF750
Allophycocyanin	NB100-65530APC
Biotin	NB100-65530B
CoraFluor 1	NB100-65530CL1
DyLight 350	NB100-65530UV
DyLight 405	NB100-65530V
DyLight 488	NB100-65530G
DyLight 550	NB100-65530R
DyLight 594	NB100-65530DL594
DyLight 650	NB100-65530C
DyLight 680	NB100-65530FR
DyLight 755	NB100-65530IR
FITC	NB100-63488
FITC	NB100-65530F
HRP	NB100-65530H
Janelia Fluor 525	NB100-65530JF525
Janelia Fluor 549	NB100-65530JF549
Janelia Fluor 646	NB100-65530JF646
Janelia Fluor 669	NB100-65530JF669
PE	NB100-65530PE
PerCP	NB100-65530PCP
mFluor Violet 450 SE	NB100-65530MFV450
mFluor Violet 500 SE	NB100-65530MFV500
mFluor Violet 610 SE	NB100-65530MFV610

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Rab13 Antibody - BSA Free

CCR10/GPR2 Antibody

CXCR7/RDC-1 Antibody

Relaxin R1/LGR7 Antibody (3E3) - Azide and BSA Free

S1P1/EDG-1 Antibody

beta-1 Adrenergic R/ADRB1 Antibody

Relaxin R2/LGR8 Antibody - BSA Free

ROR gamma/RORC/NR1F3 Antibody (4G419) - BSA Free

mGluR6 Antibody - BSA Free

Complement Component C3aR Antibody - BSA Free

Integrin alpha 5/CD49e Antibody - BSA Free

SIRP alpha/CD172a Antibody (OX-41) - BSA Free