| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-96752SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25157SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25158-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-307SS | |||
| Azide and BSA Free | NBP2-80837 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1677SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NB600-305AFP488 | |||
| Alexa Fluor Plus 555 | NB600-305AFP555 | |||
| Alexa Fluor Plus 594 | NB600-305AFP594 | |||
| Alexa Fluor Plus 647 | NB600-305AFP647 | |||
| Alexa Fluor Plus 680 | NB600-305AFP680 | |||
| FITC | NB120-6641-1000ug | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NB600-500AFP488 | |||
| Alexa Fluor Plus 555 | NB600-500AFP555 | |||
| Alexa Fluor Plus 594 | NB600-500AFP594 | |||
| Alexa Fluor Plus 647 | NB600-500AFP647 | |||
| Alexa Fluor Plus 680 | NB600-500AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05558-50ul | |||
![Immunohistochemistry: mCherry Antibody (1C51) [NBP1-96752] - Cadherin-2 is required cell autonomously for caudal migration of FBMNs. (A-I) Whole-mount immunocytochemistry showing dorsal views of Tg (isl1:GFP) (A-C) and Tg (isl1:cdh2_EC-mCherry)vc25 transgenic embryos (D-I) at 38 hpf embryos. Embryos are labeled with a-GFP (green) (A,D,G) and a-mCherry (red) (B,E,H) antibodies. (A-C) Wild-type Tg (isl1:GFP) embryos with FBMNs fully migrated into r6. (D-I) Defective caudal migration of FBMNs in Tg (isl1:GFP)/Tg(isl1:cdh2_EC-mCherry) vc25 embryos carrying one copy of the transgene (hemizygous) or two copies (homozygous). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0164433) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/mCherry-Antibody-1C51-Immunohistochemistry-NBP1-96752-img0006.jpg "Immunohistochemistry: mCherry Antibody (1C51) [NBP1-96752] - Cadherin-2 is required cell autonomously for caudal migration of FBMNs. (A-I) Whole-mount immunocytochemistry showing dorsal views of Tg (isl1:GFP) (A-C) and Tg (isl1:cdh2_EC-mCherry)vc25 transgenic embryos (D-I) at 38 hpf embryos. Embryos are labeled with a-GFP (green) (A,D,G) and a-mCherry (red) (B,E,H) antibodies. (A-C) Wild-type Tg (isl1:GFP) embryos with FBMNs fully migrated into r6. (D-I) Defective caudal migration of FBMNs in Tg (isl1:GFP)/Tg(isl1:cdh2_EC-mCherry) vc25 embryos carrying one copy of the transgene (hemizygous) or two copies (homozygous). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0164433) licensed under a CC-BY license.")
![Western Blot: mCherry Antibody (1C51) [NBP1-96752] - Analysis of HEK293 cell lysates and recombinant protein solutions using mCherry antibody, dilution 1:1,000 (Green). [1] protein standard, [2] HEK293, [3] HEK293 cells transfected with mCherry-HA construct, [4] mCherry recombinant protein, [5] GFP recombinant protein, and [6] HEK293 transfected with GFP construct. Major band at about 30 kDa corresponds to mCherry protein (predicted molecular weight: 27 kDa). mCherry antibody does not react with GFP protein. The same blot was simultaneously probed with chicken HSP60 pAb, dilution 1:5,000 in red which reveals band at 60 kDa seen only in cell lysates.](http://images.novusbio.com/fullsize/mCherry-Antibody-1C51-Western-Blot-NBP1-96752-img0005.jpg)
![Western Blot: mCherry Antibody (1C51) [NBP1-96752] - Fluorescent signals and immunoblots of the dual fluorescence reporter of cup-5 32-UTR in WT worms and mir-83(-) mutants at day 1 of adulthood. Quantification is from the western blots. GFP blots and WT worms serve as controls for loading and normalization respectively. Scale bar: 100 i1/4m. n = 3 independent experiments. Image collected and cropped by Citeab from the following publication (A secreted microRNA disrupts autophagy in distinct tissues of Caenorhabditis elegans upon ageing. Nat Commun (2019) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/mCherry-Antibody-1C51-Western-Blot-NBP1-96752-img0008.jpg)
![Western Blot: mCherry Antibody (1C51) [NBP1-96752] - Threonine 276 is required for the intramolecular interaction, and for inhibition of membrane binding. Mutation of threonine 276 to aspartic acid promotes the association of cytohesin 2 with membranes. MDCK cells were transfected with constructs encoding mCherry-tagged wild-type or T276D cytohesin 2 and fractionated into cytosol and total membranes. The fractions were Western blotted with mouse anti-mCherry, mouse anti-E-cadherin and mouse anti-actin. Image collected and cropped by CiteAb from the following publication (doi.org/10.1371/journal.pone.0082084) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/mCherry-Antibody-1C51-Western-Blot-NBP1-96752-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: mCherry Antibody (1C51) [NBP1-96752] - HEK293 cells transfected with mCherry and visualized in red. The cells were stained with NBP1-96752 in the green channel, and visualized using a confocal microscope. Transfected cells are yellow, showing overlap of the mCherry and NBP1-96752. Untransfected HEK293 cells do not express Cherry and do not stain with the antibody, but their nuclei can be visualized using a DNA stain (blue).](http://images.novusbio.com/fullsize/mCherry-Antibody-1C51-Immunocytochemistry-Immunofluorescence-NBP1-96752-img0003.jpg)
![Western Blot: mCherry Antibody (1C51) [NBP1-96752] - WB assay of the crude extract of HEK293 cells transfected with pFin-EF1-mCherry vector (lane +) and an equal amount of protein extract from untransfected HEK293 cells (lane -). NBP1-96752 binds a major band running at about 28 kDa (observed molecular weight) corresponding to intact full-length mCherry. The two other bands are clearly processed forms of mCherry as they are not present in non-transfected HEK293 cells.](http://images.novusbio.com/fullsize/mCherry-Antibody-1C51-Western-Blot-NBP1-96752-img0002.jpg)
![Immunohistochemistry-Frozen: mCherry Antibody (1C51) [NBP1-96752] - Mouse Bone Marrow Sections (Femur). Fixed-frozen and decalcified. tdTomato reporter transgenic mice. tdTomato in hematopoietic cells were detected by anti-mCherry antibody. Antibody is cross-reactive and works well for fixed-frozen bone marrow. Background is low. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/mCherry-Antibody-1C51-Immunohistochemistry-Frozen-NBP1-96752-img0004.jpg)
![Western Blot: mCherry Antibody (1C51) [NBP1-96752] - Mis18 alpha :Mis18 beta -hexamer mediates dimerization of M18BP1.(A) Analytical SEC results of M18BP11–140-MBP (cyan), M18BP11–228-MBP (red), Mis18 alpha :Mis18 beta :M18BP11–140-MBP (purple), Mis18 alpha :Mis18 beta :M18BP11–228-MBP (green). The elution volumes of thyroglobulin (670 kD), ferritin (440 kD), catalase (240 kD) & ovalbumin (44 kD) are shown as standards. Red lines indicate fractions collected for Tricine–SDS-PAGE analyses. Gels were stained with CBB. (B) Sedimentation velocity AUC results of the same samples used in the analytical SEC experiments (panel A). The best-fit size distributions are shown with the colors indicated in panel A. Data profiles used for curve-fitting analyses are shown in Figure 7—figure supplement 1. (C) Summary table of the results obtained from the AUC experiments of panel B. Sed. coef., sedimentation coefficient; MWobs., observed molecular weight; MWtheo., theoretical molecular weight. (D) Western blot results of co-immunoprecipitation experiments using GFP-Trap_A beads. HeLa CENP-A-SNAP + EGFP-M18BP11–140-P2A-T2A-mCherry-M18BP11–140, EGFP-M18BP11–140/T40D/S110E-P2A-T2A-mCherry-M18BP11–140/T40D/S110E, GST-EGFP-M18BP11–140-P2A-T2A-GST-mCherry-M18BP11–140, or GST-EGFP-M18BP11–140/T40D/S110E-P2A-T2A-GST-mCherry-M18BP11–140/T40D/S110E were analyzed.DOI://dx.doi.org/10.7554/eLife.23352.014Data profiles for AUC experiments.Best-fitting results of the sedimentation velocity AUC data of M18BP11–140-MBP, M18BP11–228-MBP, Mis18 alpha :Mis18 beta :M18BP11–140-MBP, and Mis18 alpha :Mis18 beta :M18BP11–228-MBP. Residuals represent the deviation of the continuous c(s) distribution model from the observed signals. The values of RMSD for data fitting are shown.DOI://dx.doi.org/10.7554/eLife.23352.015 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28059702), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-96752_mouse-monoclonal-mcherry-antibody-1c51-310202416113527.jpg)
![Western Blot: mCherry Antibody (1C51) [NBP1-96752] - The oligomerization state of overexpressed Cav1 varies as a function of its tag. COS-7 cells expressing the indicated constructs were lysed in digitonin & subjected to BN-PAGE followed by western blotting for Cav1 (red) & either GFP, mCherry or myc (green). A) Cells were either left untransfected (‘control’) or transfected with EGFP, Cav1-GFP or P132L-GFP. B) As in (A) except cells were transfected with the indicated mCherry constructs. C) As in (A) except cells were transfected with Cav1-myc or P132L-myc. Figures are representative of two independent experiments. Red arrows indicate the high molecular weight band positive for both tag antibodies & Cav1 antibodies (h1-97 or 2297). Black arrows indicate the high molecular weight band only positive for Cav1 antibodies (h1-97 or 2297). Green arrows indicate the low molecular weight bands only positive for FP tag antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25639341), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-96752_mouse-monoclonal-mcherry-antibody-1c51-3102024161220.jpg)
![Immunohistochemistry: mCherry Antibody [NBP2-25157] - Brn3b-mCherry expression in other regions of the CNS. Optic tracts at P0 visualized by mCherry staining (white arrows). (BAE ) Co-localization experiments with mCherry (red) and pan-Brn3 (green) antibodies at P0. (CAE ) Co-localization with mCherry (red) and Tuj1 (green) in the trigeminal ganglia at E13. 5. Arrows indicate mCherry+ Tuj+ neurons. Image collected and cropped by Citeab from the following publication (A Novel Reporter Mouse Uncovers Endogenous Brn3b Expression. Int J Mol Sci (2019)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/mCherry-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25157-img0011.jpg "Immunohistochemistry: mCherry Antibody [NBP2-25157] - Brn3b-mCherry expression in other regions of the CNS. Optic tracts at P0 visualized by mCherry staining (white arrows). (BAE ) Co-localization experiments with mCherry (red) and pan-Brn3 (green) antibodies at P0. (CAE ) Co-localization with mCherry (red) and Tuj1 (green) in the trigeminal ganglia at E13. 5. Arrows indicate mCherry+ Tuj+ neurons. Image collected and cropped by Citeab from the following publication (A Novel Reporter Mouse Uncovers Endogenous Brn3b Expression. Int J Mol Sci (2019)) licensed under a CC-BY license.")
![Immunohistochemistry: mCherry Antibody [NBP2-25157] - Activation of TRPV1 receptors in ARC POMC neurons reduces food intake. Expression of Gi/o-DREADD in POMC neurons in the ARC (top left panel, scale bar: 20 um). Arrowheads represent POMC neurons that co-expressed mCherry (n = 493 out of 529 mCherry-positive neurons, n = 3 mice). Treatment with CNO (10 uM) hyperpolarized POMC neurons (top right panel; Control, -40.7 +/- 1.5 mV; CNO, -47.1 +/- 2.3 mV, n = 6 neurons, **p 50 measurements – 3 per cell; ***P < 0.001, one‐way ANOVA on ranks followed by Dunn's post hoc test). (C) Src‐transformed p130Cas−/−MEFs co‐expressing p130Cas (SC) & mouse Flag tagged PKN3 WT or Flag‐PKN3 mPR are shown. Cells were grown on FN‐coated coverslips for 48 h, fixed, & stained for p130Cas by anti‐pTyr165 p130Cas antibody (pY165 p130Cas; 2nd 405), for actin by Phalloidin 488 & for Flag‐PKN3 by anti‐Flag antibody (2nd 633). Reflection (670 nm) indicates fibronectin degradation. All scale bars represent 20 μm. Cell were imaged by Leica TCS SP8 microscope system equipped with Leica 63×/1.45 oil objective. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30422386), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-25157_rabbit-polyclonal-mcherry-antibody-310202416114713.jpg)
![Immunocytochemistry/ Immunofluorescence: mCherry Antibody [NBP2-25157] - Brn3b-mCherry expression in the adult retina. (A) Flat-mounted retina labeled with anti-mCherry antibody. (B,B’) mCherry (red) & Brn3 (teal) colocalization. Yellow arrow indicates a Brn3+ mCherry- cell. (C,C’) mCherry (red) & RBPMS (teal) colocalization. Yellow arrows indicate RBPMS+ mCherry- cell bodies. (D,D’) mCherry (red) & Tuj1 (teal) colocalization. White arrows indicate Tuj1+ mCherry+ axons. (E–E’’’) Cross-section of an adult retina labeled with mCherry (red), RBPMS (gray), DAPI (blue), & Pax6 (green). All mCherry+ cells (white arrows) are RBPMS+. Amacrine cells are labeled with yellow stars & are mCherry- (Pax6+ RBPMS− mCherry− cells). Yellow arrowhead corresponds to an mCherry− RGC (RBPMS+ Pax6− mCherry− cell). ONH: Optic Nerve Head. ONL: Outer Nuclear Layer. INL: Inner Nuclear Layer. GCL: Ganglion Cell Layer. Scale bars: 300 microns in A, 50 microns in B–E’’’. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31197108), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-25157_rabbit-polyclonal-mcherry-antibody-310202416114789.jpg)
![Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP2-25158] - HEK293 cells transfected with mCherry, stained with mCherry antibody and viewed in a confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red, and staining with the mCherry antibody is shown in Green. The green antibody staining is only seen cells which express mCherry, and the superimposition of the green and red results in an orange signal. Interestingly stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules releasing the low molecular weight mCherry fluorochrome.](http://images.novusbio.com/fullsize/mCherry-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25158-img0005.jpg "Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP2-25158] - HEK293 cells transfected with mCherry, stained with mCherry antibody and viewed in a confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red, and staining with the mCherry antibody is shown in Green. The green antibody staining is only seen cells which express mCherry, and the superimposition of the green and red results in an orange signal. Interestingly stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules releasing the low molecular weight mCherry fluorochrome.")
![Western Blot: mCherry Antibody [NBP2-25158] - Analysis of NBP2-25158. Lane 1. Lysate of HEK293 cells transfected with pFin-EF1-mCherry vector. There is a strong clean band at about 29kDa corresponding to mCherry (predicted molecular weight ~27kDa). Lane 2, lysate of untransfected HEK293 cells show no protein bands. The NBP2-25158 antibody was used at 1:2,000 dilution.](http://images.novusbio.com/fullsize/mCherry-Antibody-NBP2-25158-img0004.jpg)
![Western Blot: mCherry Antibody [NBP2-25158] - Analysis of HEK293 cell lysates, and recombinant protein solutions using chicken mCherry pAb, dilution 1:2000 (Green). [1] protein standard, [2] HEK293, [3] HEK293 cells transfected with an mCherry-HA constract, [4] pure recombinant mCherry protein (predicted molecular weight ~ 27kDa), [5] pure recombinant GFP protein and [6] HEK293 transfected with a GFP construct. The major band at about 30kDa corresponds to mCherry-HA protein and the slightly larger recombinant form runs at about 33kDa due to presence of a His tag and other vector derived sequence. The mCherry antibody reacts strongly with both mCherry constructs and does not react with GFP protein. The blot was simultaneously probed with mouse HSP60 mAb, dilution 1:10000 (Red), which reveals a band at 60kDa only in the cell lysates.](http://images.novusbio.com/fullsize/mCherry-Antibody-Western-Blot-NBP2-25158-img0006.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP2-25158] - Dox-inducible DN Lats2-IRES-mCherry transgenic embryos were imaged before Dox treatment (top panel) and the same embryo was imaged following 24 hr of Dox live (middle panel) and fixed/stained for lineage markers (bottom panel). A representative embryo is shown for each stage. Live mCherry is shown as an extended focus image, immunofluorescence stainings shown as single plane images. mCherry positive ICMs in mosaic transgenic embryos are circled with a dotted line. Arrow points to a rare ICM cell in a 64 cell stage-induced embryo with weak Cdx2 expression, which also co-expressed an ICM marker. Scale bar: 25 um. n indicates number of transgenic embryos analyzed. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28226240/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/mCherry-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25158-img0007.jpg)
![Western blot analysis of HEK293 cell lysates using chicken pAb to mCherry, NBP2-25158, dilution 1:2,000, in green, and rabbit pAb to GAPDH, RPCA-GAPDH, dilution 1:5,000, in red: [1] protein molecular weight standard, [2] untransfected HEK293 control cells, [3] HEK293 cells transfected with pCI-Neo-mod vector expressing two tdTomato protein domains, [4] HEK293 cells transfected with pCI-Neo-mod vector expressing one mCherry-HA protein domain, and [5] HEK293 cells transfected with pCI-Neo-mod vector expressing one GFP domain. NBP2-25158 recognizes tdTomato and mCherry proteins revealing major bands at about 60kDa and 30kDa, but does not recognize GFP. The red band at 37kDa corresponds to GAPDH protein here used as a loading control.](http://images.novusbio.com/fullsize/nbp2-25158_chicken-polyclonal-mcherry-antibody-western-blot-264202414221..jpg)
![Inducible Cx43 (over-)expression in stably transfected murine G4 ES cells (clone 31), in vitro differentiation into cardiomyocytes. (a) In vitro differentiation scheme of murine ES cells into spontaneously beating EBs (adapted from Boheler et al. [33]). (b) The mCherry+ EBs derived from the transgenic Cre+ G4 ES cells of clone 31 (upper picture) and transgenic mCherry− control EBs (lower picture). Scale bars 100 um. (c) Western blot analysis of EBs from Cre+ and Cre− G4 ES cells confirmed inducible Cx43 overexpression, as underscored by P2A-tagged Cx43 and mCherry expression. (d) Immunostainings of EBs illustrated clusters of cardiac alpha -actinin+ (white) cardiomyocytes in both Cre+ (upper left picture) and Cre− control EBs (lower left picture). Immunostainings yielded strong Cx43 (green, upper left picture), P2A (white), and mCherry (red) (over-)expression (upper middle picture) in Cre+ EBs. Similar apoptotic areas preferentially in the center of Cre+ and Cre− control EBs (right pictures) were found. Scale bars 10 um. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35203340), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-25158_chicken-polyclonal-mcherry-antibody-immunocytochemistry-immunofluorescence-132202617595715.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) [NB600-307] - Reduction in CREB activity in flies following DN-CREB expression in the fat body. CRE-Luciferase reporter protein was measured using anti-luciferase antibody in Western blots of body extracts from control flies (to-GAL4 driver only, control) or flies expressing DN-CREB in the fat body from the to-GAL4 driver (DN-CREB) (top panel). Blots were stripped and reprobed with anti-tubulin antibodies as a protein loading control (bottom panel). Signal intensities were quantified and are shown as ratios to control signals (mean+/-SD, n = 5; *p<0.05, Student's t-test). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0008498) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Luciferase-Antibody-Luci-21-1-107-Western-Blot-NB600-307-img0006.jpg "Western Blot: Luciferase Antibody (Luci 21 1-107) [NB600-307] - Reduction in CREB activity in flies following DN-CREB expression in the fat body. CRE-Luciferase reporter protein was measured using anti-luciferase antibody in Western blots of body extracts from control flies (to-GAL4 driver only, control) or flies expressing DN-CREB in the fat body from the to-GAL4 driver (DN-CREB) (top panel). Blots were stripped and reprobed with anti-tubulin antibodies as a protein loading control (bottom panel). Signal intensities were quantified and are shown as ratios to control signals (mean+/-SD, n = 5; *p<0.05, Student's t-test). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0008498) licensed under a CC-BY license.")
![Immunohistochemistry: Luciferase Antibody (Luci 21 1-107) [NB600-307] - Detection of Luciferase expression in CD34+ cells by Immunohistochemistry. Cytospin slides prepared from transduced CD34+ cells after 3 days of culture were stained with monoclonal anti-Luciferase antibody. Luciferase-positive cells have green cytoplasm; nuclei stained with DAPI are blue. Nontransduced, cultured CD34+ cells were used as a negative control. Original magnification, 40X. Wang, X. et al., Dynamic tracking of human hematopoietic stem cell. Blood. 102(10): 3478-3482, 2003.](http://images.novusbio.com/fullsize/Luciferase-Antibody-Luci-21-1-107-Immunohistochemistry-NB600-307-img0004.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) [NB600-307] - WB analysis of NB600-307 on Luciferase Protein (NB810-74573)](http://images.novusbio.com/fullsize/Luciferase-Antibody-Luci-21-1-107-Western-Blot-NB600-307-img0005.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - TTFields application leads to increased autophagic flux.a Ultra-structural STEM electron microscopy analysis of U-87 MG (upper panel) & A172 (lower panel) cells treated with TTFields for 48 h. Autophagosomes (blue arrows) & autolysosomes (green arrows) are indicated. b U-87 MG & A172 cells were either left untreated or were treated with TTFields for 24–72 h. CQ (20 µM) was added 4 h before cells were collected. Samples were immunoblotted for LC3 & GAPDH. Upper panel: representative blots. Lower panel: densitometric quantification of immunoblot signal, showing an average of at least three independent experiments (0.01 < *P < 0.05, Student’s t-test). c U-87 MG & A172 cells were either left untreated or were treated with TTFields for 48 h. CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 Ab (green) & DAPI (blue). Upper panel: representative images. Original magnifications: × 40. Lower panel: quantification of LC3 intensity, presented as average intensity per cell (**P < 0.01, Student’s t-test). d U-87 MG cells were either left untreated or were treated with TTFields (48 h) or with vinblastin 25 nM (0.5 h). CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 (green), LAMP1 (red), & DAPI (blue) (upper panel). Arrowheads indicate the strongest colocalization staining in each cell. Intensity histograms of LAMP1 & LC3 fluorescent signal calculated from the region of interest indicated by the white bar (lower panel). Representative images are shown Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-310202416171421.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - Induction of autophagy by TTFields is AMPK dependent.a U-87 MG & A172 cells either left untreated or treated w/ TTFields for indicated time points. Immunoblot analysis of GFP78 protein. Numeric values represent fold increase in GRP78 signal, normalized to loading control (GAPDH), relative to untreated control. b Quantification of intracellular ATP levels in U-87 MG cells either left untreated or treated w/ TTFields for 72 h.Results presented as average ATP concentration (nmol/2 × 106 cells) from 3 independent experiments (*P < 0.01, Student’s t-test). c U-87 MG & A172 cells either left untreated or treated w/ TTFields for indicated time points. Immunoblot analysis of pAMPK & pULK1proteins. GAPDH used as loading control. (5D-F) U-87 MG cells transfected w/ AMPK-targeting siRNA (siAMPK) or w/ siRNA sham vector (siVector), & incubated for 48 h w/ or w/out TTFields application. CQ 20 µM added for last 4 h of treatment where indicated. d (left panel) Immunoblot analysis of LC3 & AMPK. Numeric values represent fold-change in LC3-II signal, normalized to GAPDH signal, relative to respective control. d (right panel) CQ-treated cells fixed & stained for LC3 (green) & DAPI (blue), original magnifications: × 40. e Cell count of siAMPK- or siVector-expressing cells after TTFields treatment. (0.01 < *P < 0.05, Student’s t-test, n = 3). f siVector- & siAMPK-transfected U-87 MG cells either left untreated or treated w/ TTFields for 48 h. Cells then fixed & stained for cleaved caspase-3 (green) & DAPI (blue) (left panel). Images from each treatment analyzed manually & fraction of cleaved caspase-3-positive cells calculated for at least 200 cells from each group (right panel) (**P < 0.01, Student’s t-test, n = 2) Image collected & cropped by CiteAb from following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-31020241616016.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - TTFields induce autophagy in glioma cell lines.a U-87 MG & A172 cells were either left untreated or treated with TTFields at the last 24 h, 48 h, or 72 h of culturing. All cultures were plated on the same time, incubated overnight to allow cell attachment, & collected 72 h afterwards. Cells were collected, lysed, & samples were analyzed using immunoblotting for LC3 & GAPDH. Upper panel: representative blots. Lower panel: densitometric quantification of immunoblot signal, showing an average of at least three independent experiments (0.01 < *P < 0.05, **P < 0.01, Student’s t-test). b Paraffin-embedded sections from sham- or TTFields-treated rats were stained with anti-LC3 Ab (green) & DAPI (blue). Representative images are presented. c Quantification of LC3 intensity, presented as fold increase from corresponding control (*P < 0.05, Student’s t-test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-310202416163711.jpg)
![Immunocytochemistry/ Immunofluorescence: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - Immunofluorescence imaging of luciferase protein as a reporter of hybrid formation. Entire primary tumor & lung sections were imaged via tile scanning, & each image of the scan was carefully analyzed to confirm or refute positive staining for luciferase. The luciferase signal was considered a positive signal if it was above background levels associated with negative controls & corresponded to the cytoplasm of a cell with a nucleus. Rare luciferase-positive cells were detected in the primary tumors. Most red signal was not in the cytoplasm of cells associated with nuclei and, therefore, considered nonspecific [insets (a), (c)]. The lungs containing metastases on the other hand [(b), (d)] contained a large number of bona fide luciferase-positive cells corresponding to fusion products. Scale bars on slide scans = 100 μm. Scale bars on 40× inset = 25 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31069316), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-31020241618921.jpg)
![Western Blot: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - TTFields induce autophagy in glioma cell lines.a U-87 MG & A172 cells were either left untreated or treated with TTFields at the last 24 h, 48 h, or 72 h of culturing. All cultures were plated on the same time, incubated overnight to allow cell attachment, & collected 72 h afterwards. Cells were collected, lysed, & samples were analyzed using immunoblotting for LC3 & GAPDH. Upper panel: representative blots. Lower panel: densitometric quantification of immunoblot signal, showing an average of at least three independent experiments (0.01 < *P < 0.05, **P < 0.01, Student’s t-test). b Paraffin-embedded sections from sham- or TTFields-treated rats were stained with anti-LC3 Ab (green) & DAPI (blue). Representative images are presented. c Quantification of LC3 intensity, presented as fold increase from corresponding control (*P < 0.05, Student’s t-test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-310202416163757.jpg)
![Immunocytochemistry/ Immunofluorescence: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - TTFields application leads to increased autophagic flux.a Ultra-structural STEM electron microscopy analysis of U-87 MG (upper panel) & A172 (lower panel) cells treated with TTFields for 48 h. Autophagosomes (blue arrows) & autolysosomes (green arrows) are indicated. b U-87 MG & A172 cells were either left untreated or were treated with TTFields for 24–72 h. CQ (20 µM) was added 4 h before cells were collected. Samples were immunoblotted for LC3 & GAPDH. Upper panel: representative blots. Lower panel: densitometric quantification of immunoblot signal, showing an average of at least three independent experiments (0.01 < *P < 0.05, Student’s t-test). c U-87 MG & A172 cells were either left untreated or were treated with TTFields for 48 h. CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 Ab (green) & DAPI (blue). Upper panel: representative images. Original magnifications: × 40. Lower panel: quantification of LC3 intensity, presented as average intensity per cell (**P < 0.01, Student’s t-test). d U-87 MG cells were either left untreated or were treated with TTFields (48 h) or with vinblastin 25 nM (0.5 h). CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 (green), LAMP1 (red), & DAPI (blue) (upper panel). Arrowheads indicate the strongest colocalization staining in each cell. Intensity histograms of LAMP1 & LC3 fluorescent signal calculated from the region of interest indicated by the white bar (lower panel). Representative images are shown Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-31020241616033.jpg)
![Immunocytochemistry/ Immunofluorescence: Luciferase Antibody (Luci 21 1-107) - BSA Free [NB600-307] - TTFields application leads to increased autophagic flux.a Ultra-structural STEM electron microscopy analysis of U-87 MG (upper panel) & A172 (lower panel) cells treated with TTFields for 48 h. Autophagosomes (blue arrows) & autolysosomes (green arrows) are indicated. b U-87 MG & A172 cells were either left untreated or were treated with TTFields for 24–72 h. CQ (20 µM) was added 4 h before cells were collected. Samples were immunoblotted for LC3 & GAPDH. Upper panel: representative blots. Lower panel: densitometric quantification of immunoblot signal, showing an average of at least three independent experiments (0.01 < *P < 0.05, Student’s t-test). c U-87 MG & A172 cells were either left untreated or were treated with TTFields for 48 h. CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 Ab (green) & DAPI (blue). Upper panel: representative images. Original magnifications: × 40. Lower panel: quantification of LC3 intensity, presented as average intensity per cell (**P < 0.01, Student’s t-test). d U-87 MG cells were either left untreated or were treated with TTFields (48 h) or with vinblastin 25 nM (0.5 h). CQ (20 µM) was added at the last 3 h of treatment & cells were fixed & stained with anti-LC3 (green), LAMP1 (red), & DAPI (blue) (upper panel). Arrowheads indicate the strongest colocalization staining in each cell. Intensity histograms of LAMP1 & LC3 fluorescent signal calculated from the region of interest indicated by the white bar (lower panel). Representative images are shown Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30341282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-307_mouse-monoclonal-luciferase-antibody-luci-21-1-107-310202416175224.jpg)
![Western Blot: beta Galactosidase Antibody [NB600-305] - Analysis using FITC conjugate of NB600-305. 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting. The membrane was probed with fluorescein conjugated anti-b-Galactosidase diluted to 1:10,000. Reaction occurred for 2 hours at room temperature.](http://images.novusbio.com/fullsize/beta-Galactosidase-Antibody-Western-Blot-NB600-305-img0017.jpg "Western Blot: beta Galactosidase Antibody [NB600-305] - Analysis using FITC conjugate of NB600-305. 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting. The membrane was probed with fluorescein conjugated anti-b-Galactosidase diluted to 1:10,000. Reaction occurred for 2 hours at room temperature.")
![Western Blot: beta Galactosidase Antibody [NB600-305] - Analysis of a band at 117 kDa (lane 1) corresponding to b-Gal present in a partially purified preparation (arrowhead). Approximately 1 ug of protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4 C followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature (800 nm channel, green).](http://images.novusbio.com/fullsize/beta-Galactosidase-Antibody-Western-Blot-NB600-305-img0014.jpg)
![Western Blot: beta Galactosidase Antibody [NB600-305] - Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under reducing conditions are shown on the right. Blots were blocked overnight at 4 C with Blocking Buffer for Fluorescent Western Blotting. The membrane was probed with anti-b-Galactosidase diluted to 1:10,000. Reaction occurred overnight at 4C. Dylight649 conjugated Gt-a-anti-Rabbit IgG was used for detection.](http://images.novusbio.com/fullsize/beta-Galactosidase-Antibody-Western-Blot-NB600-305-img0015.jpg)
![Western Blot: beta Galactosidase Antibody [NB600-305] - Shows a band at 117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting. The membrane was probed with fluorescein conjugated anti-b-Galactosidase diluted to 1:10,000. Reaction occurred for 2 hours at room temperature.](http://images.novusbio.com/fullsize/beta-Galactosidase-Antibody-Western-Blot-NB600-305-img0016.jpg)
![Western Blot of Rabbit Anti-Beta-Galactosidase Antibody. Lane 1: Beta-Galactosidase Reduced [0.1ug]. Lane 2: Opal Prestained Molecular Weight Marker](http://images.novusbio.com/fullsize/nb600-305_rabbit-polyclonal-beta-galactosidase-antibody-24520231056468.jpg)
![Western Blot of Rabbit Anti-Beta-Galactosidase Antibody. Lane 1: partially purified preparation b-Galactosidase [1.0ug]. Lane 2: Molecular Weight Marker. Primary Antibody: Anti-Beta-Galactosidase at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG IRDye(R)800](http://images.novusbio.com/fullsize/nb600-305_rabbit-polyclonal-beta-galactosidase-antibody-2552023131130.jpg)
![Western Blot: mCherry Antibody [NBP2-43720] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody (NBP2-43720) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43720_rabbit-polyclonal-mcherry-antibody-9102024205672.jpg "Western Blot: mCherry Antibody [NBP2-43720] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody (NBP2-43720) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunoprecipitation: mCherry Antibody [NBP2-43720] - Analysis of 293T whole cell lysate/extractA : 20 ug whole cell lysate/extract of mcherry protein expressing 293T cellsB : Control with 2.5 ug of pre-immune rabbit IgGC : Immunoprecipitation of mcherry protein by 2.5 ug of mcherry antibody 7.5% SDS-PAGE The immunoprecipitated mcherry protein was detected by mcherry antibody diluted at 1 : 3000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/mCherry-Antibody-Immunoprecipitation-NBP2-43720-img0002.jpg)
![Western Blot: mCherry Antibody [NBP2-43720] - Analysis of A. 5ng recombinant mCherry B. 10ng recombinant mCherry C. 20ng recombinant mCherry 12 % SDS-PAGE mCherry antibody dilution: 1:2000.](http://images.novusbio.com/fullsize/mCherry-Antibody-Western-Blot-NBP2-43720-img0004.jpg)
![Western Blot: mCherry Antibody [NBP2-43720] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with mCherry antibody (NBP2-43720) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43720_rabbit-polyclonal-mcherry-antibody-91020242014626.jpg)
![Western Blot: mCherry Antibody (235) [NBP2-43768] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of mCherry-tagged protein expressing 293T cells 5 % SDS-PAGE mCherry antibody [235] dilution: 1:1000.](http://images.novusbio.com/fullsize/mCherry-Antibody-235-Western-Blot-NBP2-43768-img0001.jpg "Western Blot: mCherry Antibody (235) [NBP2-43768] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of mCherry-tagged protein expressing 293T cells 5 % SDS-PAGE mCherry antibody [235] dilution: 1:1000.")
![Western Blot: Alkaline Phosphatase, Bacterial Antibody [Biotin] [NB600-500] - Lane 1: Alkaline Phosphatase (E. Coli). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (E. Coli) Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for Alkaline Phosphatase (E. Coli).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Bacterial-Antibody-[Biotin]-Western-Blot-NB600-500-img0003.jpg "Western Blot: Alkaline Phosphatase, Bacterial Antibody [Biotin] [NB600-500] - Lane 1: Alkaline Phosphatase (E. Coli). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (E. Coli) Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for Alkaline Phosphatase (E. Coli).")
![Immunoprecipitation: mCherry Antibody [NBP2-43727] - Analysis of 293T whole cell lysate/extractA : 20 ug whole cell lysate/extract of mcherry protein expressing 293T cellsB : Control with 2.5 ug of pre-immune rabbit IgGC : Immunoprecipitation of mcherry protein by 2.5 ug of mcherry antibody 7.5% SDS-PAGE The immunoprecipitated mcherry protein was detected by mcherry antibody diluted at 1 : 3000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/mCherry-Antibody-Immunoprecipitation-NBP2-43727-img0001.jpg "Immunoprecipitation: mCherry Antibody [NBP2-43727] - Analysis of 293T whole cell lysate/extractA : 20 ug whole cell lysate/extract of mcherry protein expressing 293T cellsB : Control with 2.5 ug of pre-immune rabbit IgGC : Immunoprecipitation of mcherry protein by 2.5 ug of mcherry antibody 7.5% SDS-PAGE The immunoprecipitated mcherry protein was detected by mcherry antibody diluted at 1 : 3000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.")
![Immunocytochemistry/ Immunofluorescence: Luciferase Antibody [NBP2-19295] - Firefly Luciferase antibody detects Firefly Luciferase protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Firefly Luciferase stained by Firefly Luciferase antibody diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp2-19295_rabbit-polyclonal-luciferase-antibody-immunocytochemistry-immunofluorescence-298202414621..jpg "Immunocytochemistry/ Immunofluorescence: Luciferase Antibody [NBP2-19295] - Firefly Luciferase antibody detects Firefly Luciferase protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Firefly Luciferase stained by Firefly Luciferase antibody diluted at 1:500.Blue: Fluoroshield with DAPI.")
![Western Blot: Luciferase Antibody [NBP2-19295] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Firefly Luciferase antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-19295_rabbit-polyclonal-luciferase-antibody-western-blot-298202414820..jpg)
![Western Blot: mCherry Antibody [NBP3-05558] - Western blot analysis of HEK293 cell lysates using mCherry Antibody at a dilution of 1:2,000, in green [1] protein standard, [2] HEK293 control cells, [3] HEK293 cells stably transduced with a lentiviral vector expressing mCherry-HA construct, and [4] HEK293 cells transfected with mCherry-HA construct. Major band at about 30kDa corresponds to mCherry protein.](http://images.novusbio.com/fullsize/mCherry-Antibody-Western-Blot-NBP3-05558-img0002.jpg "Western Blot: mCherry Antibody [NBP3-05558] - Western blot analysis of HEK293 cell lysates using mCherry Antibody at a dilution of 1:2,000, in green [1] protein standard, [2] HEK293 control cells, [3] HEK293 cells stably transduced with a lentiviral vector expressing mCherry-HA construct, and [4] HEK293 cells transfected with mCherry-HA construct. Major band at about 30kDa corresponds to mCherry protein.")
![Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP3-05558] - Immunofluorescent analysis of HEK293 cells transfected with mCherry-HA construct (red) and stained with mCherry Antibody at a dilution of 1:2,000, in green. The blue is Hoechst staining of nuclear DNA. The mCherry antibody reveals mCherry protein expressed only in transfected cells which appear golden in color. Untransfected cells expressing no mCherry are not recognized by the antibody, as a result only their nuclei are visible.](http://images.novusbio.com/fullsize/mCherry-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05558-img0001.jpg)