Western Blot: mCherry Antibody (1C51) [NBP1-96752] - WB assay of the crude extract of Hek293 cells transfected with pFin-EF1-mCherry vector (lane +) and an equal amount of protein extract from untransfected Hek293 ...read more
Immunocytochemistry/ Immunofluorescence: mCherry Antibody (1C51) [NBP1-96752] - shows Hek293 cells transfected with mCherry and visualized in red. The cells were stained with NBP1-96752 in the green channel, and ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
10mM Sodium Azide
Protein G purified
Western Blot 1:2000
This mCherry (1C51) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 28 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry reported in scientific literature (PMID 27396338).
Read Publications using NBP1-96752 in the following applications:
mCherry represents an engineered derivative of a family of proteins initially isolated from a group of sea organisms called Cnidarians (jelly fish, sea anemones and corals). The prototype for these fluorescent proteins is GFP which was originally isolated from jellyfish Aequoria Victoria and when expressed in-vitro or in-vivo using its gene, GFP protein fold correctly with retention of its capability to deliver strong fluorescence. Engineered forms of GFP and its relatives have been developed to monitor several biological proresses such as Ca2+ levels, protease activation and a variety of other processes in real time. mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of Discosoma species. DsRed is similar in size and other properties to GFP, but, produces a red rather than a green fluoresnecce and its engineered monomeric form has several applications including FRET (Forster Resonance Energy Transfer). mCherry was engineered with further multiple cycles of mutation, directed modification and evolutionary selection, and it is also a monomeric form with an excitation maximum at 587 nm and an emission maximum at 610 nm.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for mCherry Antibody (NBP1-96752)(14)
We have publications tested in 2 confirmed species: Human, Mouse.
We have publications tested in 3 applications: ICC/IF, IHC, WB.
FAQs for mCherry Antibody (NBP1-96752). (Showing 1 - 4 of 4 FAQs).
Would this antibody detect DsRed?
NBP1-45840, NBP1-97373 and NBP1-97371 will all recognize DsRed. I have no information about its cross-reactivity of DsRed with NBP1-96752.
Do you have any data on the use of NBP1-96752 for immunohistochemistry?
At this time we do not have any date on the use of NBP1-96752 in Immunohistochemistry. If you would be interested in testing this antibody, I would invite you to take a look at our Innovator's Reward Program.
Regarding RFP Antibody (NBP1-97373). Does it recognize dTomato?
I am sorry, but product NBP1-97373 has been discontinued. However, I have now received confirmation from the lab that mCherry Antibody (1C51) NBP1-96752 does indeed bind to dTomato, and so we would recommend this antibody for you.
Regarding the mCherry antibody (NBP1-96752) I would like to know if it also detects RFP
We have not tested our mCherry antibody with catalogue number NBP1-96752 against RFP, however since the two proteins share a high degree of sequence homology the antibody is likely to recognise RFP. As we have not performed this testing in house however, we cannot guarantee that NBP1-96752 will or will not cross-react with RFP.