mCherry Antibody (1C51)


Western Blot: mCherry Antibody (1C51) [NBP1-96752] - WB assay of the crude extract of Hek293 cells transfected with pFin-EF1-mCherry vector (lane +) and an equal amount of protein extract from untransfected Hek293 more
Immunocytochemistry/ Immunofluorescence: mCherry Antibody (1C51) [NBP1-96752] - shows Hek293 cells transfected with mCherry and visualized in red. The cells were stained with NBP1-96752 in the green channel, and more

Product Details

Reactivity AllSpecies Glossary
Applications WB, IHC, ICC
1 mg/ml

mCherry Antibody (1C51) Summary

This antibody is raised against recombinant full-length mCherry with His tag purified from E. coli.
Does not cross react with GFP.
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
10mM Sodium Azide
1 mg/ml
Protein G purified


  • Western Blot 1:2000
  • Immunohistochemistry
  • Immunocytochemistry 1:500
Application Notes
This mCherry (1C51) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 28 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry reported in scientific literature (PMID 27396338).
Theoretical MW
28 kDa
Read Publications using
NBP1-96752 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    10 publications

Alternate Names for mCherry Antibody (1C51)

  • red fluorescent protein mCherry
  • Red Fluoroscent Protein


mCherry represents an engineered derivative of a family of proteins initially isolated from a group of sea organisms called Cnidarians (jelly fish, sea anemones and corals). The prototype for these fluorescent proteins is GFP which was originally isolated from jellyfish Aequoria Victoria and when expressed in-vitro or in-vivo using its gene, GFP protein fold correctly with retention of its capability to deliver strong fluorescence. Engineered forms of GFP and its relatives have been developed to monitor several biological proresses such as Ca2+ levels, protease activation and a variety of other processes in real time. mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of Discosoma species. DsRed is similar in size and other properties to GFP, but, produces a red rather than a green fluoresnecce and its engineered monomeric form has several applications including FRET (Forster Resonance Energy Transfer). mCherry was engineered with further multiple cycles of mutation, directed modification and evolutionary selection, and it is also a monomeric form with an excitation maximum at 587 nm and an emission maximum at 610 nm.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for mCherry Antibody (NBP1-96752)(14)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC, WB.

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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NBP1-96752 Applications Species
Cho A, Kato M, Whitwam T et al. An Atypical Tropomyosin in Drosophila with Intermediate Filament-like Properties Cell Rep 2016 Jul 26 [PMID:27396338] (IHC) IHC
Estruch SB, Graham SA, Deriziotis P, Fisher SE. The language-related transcription factor FOXP2 is post-translationally modified with small ubiquitin-like modifiers. Sci Rep. 2016 Feb 12 [PMID:26867680] (WB, Human) WB Human
Ruas M, Davis LC, Chen CC et al. Expression of Ca2+-permeable two-pore channels rescues NAADP signalling in TPC-deficient cells EMBO J. 2015 Apr 14 [PMID:25872774] (ICC/IF, Mouse) ICC/IF Mouse
Zhu M, Li X, Tian X, Wu C. Mask loss-of-function rescues mitochondrial impairment and muscle degeneration of Drosophila pink1 and parkin mutants Hum. Mol. Genet. 2015 Mar 05 [PMID:25743185] (WB) WB
Han B, Tiwari A, Kenworthy AK. Tagging strategies strongly affect the fate of overexpressed caveolin-1. Traffic 2015 Apr [PMID:25639341]
Yousef H, Morgenthaler A, Schlesinger C et al. Age-Associated Increase in BMP Signaling inhibits Hippocampal Neurogenesis. Stem Cells. 2014 Dec 23 [PMID:25538007]
Andrews OE,Cha DJ,Wei C, Patton JG. RNAi-Mediated Gene silencing in Zebrafish Triggered by Convergent Transcription Sci Rep. 2014 Jun 09 [PMID:24909225] (WB) WB
Liang Hongqing, Esposito Alessandro, De Siddharth et al. Homeostatic control of polo-like kinase-1 engenders non-genetic heterogeneity in G2 checkpoint fidelity and timing. Nat Commun. 2014 Jun 04 [PMID:24893992] (WB) WB
Liang S, Varrecchia M, Ishida K, Jolly ER. Evaluation of schistosome promoter expression for transgenesis and genetic analysis. PLoS ONE. 2014 May 26 [PMID:24858918] (WB) WB
Li Y, Low LH, Putz U et al. Rab5 and Ndfip1 are involved in Pten ubiquitination and nuclear trafficking. Traffic. 2014 May 05 [PMID:24798731] (WB) WB
Show All 14 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for mCherry Antibody (NBP1-96752). (Showing 1 - 4 of 4 FAQs).

  1. Would this antibody detect DsRed?
    • NBP1-45840, NBP1-97373 and NBP1-97371 will all recognize DsRed. I have no information about its cross-reactivity of DsRed with NBP1-96752.
  2. Do you have any data on the use of NBP1-96752 for immunohistochemistry?
    • At this time we do not have any date on the use of NBP1-96752 in Immunohistochemistry. If you would be interested in testing this antibody, I would invite you to take a look at our Innovator's Reward Program.
  3. Regarding RFP Antibody (NBP1-97373). Does it recognize dTomato?
    • I am sorry, but product NBP1-97373 has been discontinued. However, I have now received confirmation from the lab that mCherry Antibody (1C51) NBP1-96752 does indeed bind to dTomato, and so we would recommend this antibody for you.
  4. Regarding the mCherry antibody (NBP1-96752) I would like to know if it also detects RFP
    • We have not tested our mCherry antibody with catalogue number NBP1-96752 against RFP, however since the two proteins share a high degree of sequence homology the antibody is likely to recognise RFP. As we have not performed this testing in house however, we cannot guarantee that NBP1-96752 will or will not cross-react with RFP.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP1-96752AF405
Alexa Fluor (R) 488 Labeled NBP1-96752AF488
Alexa Fluor (R) 647 Labeled NBP1-96752AF647
Alexa Fluor (R) 700 Labeled NBP1-96752AF700
DyLight 350 Labeled NBP1-96752UV
DyLight 405 Labeled NBP1-96752V
DyLight 405LS Labeled NBP1-96752V3
DyLight 488 Labeled NBP1-96752G
DyLight 680 Labeled NBP1-96752FR
DyLight 755 Labeled NBP1-96752IR
FITC Labeled NBP1-96752F

Additional mCherry Antibody (1C51) Products

mCherry NBP1-96752

Blogs on mCherry

There are no specific blogs for mCherry, but you can read our latest blog posts.

Contact Information

Product PDFs

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