mCherry Antibody (1C51)

Images

 
Immunocytochemistry/ Immunofluorescence: mCherry Antibody (1C51) [NBP1-96752] - shows Hek293 cells transfected with mCherry and visualized in red. The cells were stained with NBP1-96752 in the green channel, and ...read more
Western Blot: mCherry Antibody (1C51) [NBP1-96752] - WB assay of the crude extract of Hek293 cells transfected with pFin-EF1-mCherry vector (lane +) and an equal amount of protein extract from untransfected Hek293 ...read more

Product Details

Summary
Reactivity AllSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, ICC
Clone
1C51
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

mCherry Antibody (1C51) Summary

Immunogen
This antibody is raised against recombinant full-length mCherry with His tag purified from E. coli.
Specificity
Does not cross react with GFP.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Immunocytochemistry 1:500
Application Notes
This mCherry (1C51) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 28 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry reported in scientific literature (PMID 27396338). Use in Immunohistochemistry-paraffin reported in scientific literature (PMID 27716840). Use in immunoprecipitation reported in scientific literature (PMID 28059702).
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-96752 in the following applications:

Publications
Read Publications using
NBP1-96752 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
10mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for mCherry Antibody (1C51)

  • DSRED
  • red fluorescent protein mCherry
  • Red Fluoroscent Protein

Background

mCherry represents an engineered derivative of a family of proteins initially isolated from a group of sea organisms called Cnidarians (jelly fish, sea anemones and corals). The prototype for these fluorescent proteins is GFP which was originally isolated from jellyfish Aequoria Victoria and when expressed in-vitro or in-vivo using its gene, GFP protein fold correctly with retention of its capability to deliver strong fluorescence. Engineered forms of GFP and its relatives have been developed to monitor several biological proresses such as Ca2+ levels, protease activation and a variety of other processes in real time. mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of Discosoma species. DsRed is similar in size and other properties to GFP, but, produces a red rather than a green fluoresnecce and its engineered monomeric form has several applications including FRET (Forster Resonance Energy Transfer). mCherry was engineered with further multiple cycles of mutation, directed modification and evolutionary selection, and it is also a monomeric form with an excitation maximum at 587 nm and an emission maximum at 610 nm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for mCherry Antibody (NBP1-96752)(23)

We have publications tested in 4 confirmed species: Human, Mouse, Insect, Zebrafish.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-P
(1)
WB
(18)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
Insect
(1)
Zebrafish
(1)
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Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NBP1-96752 Applications Species
Gaynor AS, Chen W. Induced prodrug activation by conditional protein degradation J. Biotechnol. 2017 Oct 20 [PMID: 28912080] (WB) WB
Weir NR, Kamber RA, Martenson JS, Denic V. The AAA protein Msp1 mediates clearance of excess tail-anchored proteins from the peroxisomal membrane Elife 2017 Sep 14 [PMID: 28906250] (WB) WB
Zhu M, Zhang S, Tian X, Wu C. Mask mitigates MAPT- and FUS-induced degeneration by enhancing autophagy through lysosomal acidification Autophagy 2017 Aug 14 [PMID: 28806139] (WB, Insect) WB Insect
Fan Y, Evans CR, Barber KW et al. Heterogeneity of Stop Codon Readthrough in Single Bacterial Cells and Implications for Population Fitness Mol. Cell 2017 Aug 01 [PMID: 28781237] (WB) WB
Tan SLW, Chadha S, Liu Y et al. A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability. Cell. 2017 Jun 01 [PMID: 28575672] (WB, Human) WB Human
Pan D, Klare K, Petrovic A et al. CDK-regulated dimerization of M18BP1 on a Mis18 hexamer is necessary for CENP-A loading Elife 2017 Jan 6 [PMID: 28059702] (WB, Human) WB Human
Rebman JK, Kirchoff KE, Walsh GS. Cadherin-2 Is Required Cell Autonomously for Collective Migration of Facial Branchiomotor Neurons. PLoS ONE Oct 7 2016 [PMID: 27716840] (IHC-P, Zebrafish) IHC-P Zebrafish
Cho A, Kato M, Whitwam T et al. An Atypical Tropomyosin in Drosophila with Intermediate Filament-like Properties Cell Rep 2016 Jul 26 [PMID: 27396338] (IHC) IHC
Li X, Rydzewski N, Hider A et al. A molecular mechanism to regulate lysosome motility for lysosome positioning and tubulation. Nat Cell Biol 2016 Apr [PMID: 26950892] (WB) WB
Estruch SB, Graham SA, Deriziotis P, Fisher SE. The language-related transcription factor FOXP2 is post-translationally modified with small ubiquitin-like modifiers. Sci Rep. 2016 Feb 12 [PMID: 26867680] (WB, Human) WB Human
Show All 23 Publications.

Review for mCherry Antibody (NBP1-96752) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-96752:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 03/17/2017
View

Summary

ApplicationWestern Blot
Sample Tested Hela whole cell lysate,HeLa transfected with mCherry containing compound,HeLa transfected with mCherry-tagged compound
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for mCherry Antibody (NBP1-96752). (Showing 1 - 4 of 4 FAQs).

  1. Would this antibody detect DsRed?
    • NBP1-45840, NBP1-97373 and NBP1-97371 will all recognize DsRed. I have no information about its cross-reactivity of DsRed with NBP1-96752.
  2. Do you have any data on the use of NBP1-96752 for immunohistochemistry?
    • At this time we do not have any date on the use of NBP1-96752 in Immunohistochemistry. If you would be interested in testing this antibody, I would invite you to take a look at our Innovator's Reward Program.
  3. Regarding RFP Antibody (NBP1-97373). Does it recognize dTomato?
    • I am sorry, but product NBP1-97373 has been discontinued. However, I have now received confirmation from the lab that mCherry Antibody (1C51) NBP1-96752 does indeed bind to dTomato, and so we would recommend this antibody for you.
  4. Regarding the mCherry antibody (NBP1-96752) I would like to know if it also detects RFP
    • We have not tested our mCherry antibody with catalogue number NBP1-96752 against RFP, however since the two proteins share a high degree of sequence homology the antibody is likely to recognise RFP. As we have not performed this testing in house however, we cannot guarantee that NBP1-96752 will or will not cross-react with RFP.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-96752AF405
Alexa Fluor 488 Labeled NBP1-96752AF488
Alexa Fluor 647 Labeled NBP1-96752AF647
Alexa Fluor 700 Labeled NBP1-96752AF700
DyLight 350 Labeled NBP1-96752UV
DyLight 405 Labeled NBP1-96752V
DyLight 488 Labeled NBP1-96752G
DyLight 680 Labeled NBP1-96752FR
DyLight 755 Labeled NBP1-96752IR
FITC Labeled NBP1-96752F

Additional mCherry Products

mCherry NBP1-96752

Blogs on mCherry

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Recent Reviews

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Anonymous
03/17/2017
Application: WB
Species: Human