mCherry Antibody (1C51)

Images

 
Immunohistochemistry: mCherry Antibody (1C51) [NBP1-96752] - Cadherin-2 is required cell autonomously for caudal migration of FBMNs. (A-I) Whole-mount immunocytochemistry showing dorsal views of Tg (isl1:GFP) (A-C) and ...read more
Western Blot: mCherry Antibody (1C51) [NBP1-96752] - Analysis of HEK293 cell lysates and recombinant protein solutions using mCherry antibody, dilution 1:1,000 (Green). [1] protein standard, [2] HEK293, [3] HEK293 cells ...read more
Western Blot: mCherry Antibody (1C51) [NBP1-96752] - Fluorescent signals and immunoblots of the dual fluorescence reporter of cup-5 32-UTR in WT worms and mir-83(-) mutants at day 1 of adulthood. Quantification is from ...read more
Western Blot: mCherry Antibody (1C51) [NBP1-96752] - Threonine 276 is required for the intramolecular interaction, and for inhibition of membrane binding. Mutation of threonine 276 to aspartic acid promotes the ...read more
Immunocytochemistry/ Immunofluorescence: mCherry Antibody (1C51) [NBP1-96752] - HEK293 cells transfected with mCherry and visualized in red. The cells were stained with NBP1-96752 in the green channel, and visualized ...read more
Western Blot: mCherry Antibody (1C51) [NBP1-96752] - WB assay of the crude extract of HEK293 cells transfected with pFin-EF1-mCherry vector (lane +) and an equal amount of protein extract from untransfected HEK293 ...read more
Immunohistochemistry-Frozen: mCherry Antibody (1C51) [NBP1-96752] - Mouse Bone Marrow Sections (Femur). Fixed-frozen and decalcified. tdTomato reporter transgenic mice. tdTomato in hematopoietic cells were detected by ...read more

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO, Single-Cell Western
Clone
1C51
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

mCherry Antibody (1C51) Summary

Immunogen
This mCherry Antibody (1C51) was developed against recombinant full-length mCherry purified from E. coli.
Specificity
This mCherry Antibody (1C51) does not cross react with GFP.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockout Validated
  • Single Cell Western 100 ug/mL
  • Western Blot 1:1000 - 1:2000
Application Notes
Use in Flow reported in scientific literature (PMID:33335127). Use in IHC and IHC-P reported in scientific literature (PMID: 27396338 and 27716840 respectively).
mCherry antibody validated for IHC-Frozen from a verified customer review.
Use in Immunoprecipitation reported in scientific literature (PMID: 33008892).
Use in Knockout Validation was reported in scientific literature (PMID: 32547960).
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-96752 in the following applications:

  • 1 publication
  • 5 publications
  • IHC
    4 publications
  • 2 publications
  • IP
    2 publications
  • KO
    1 publication
  • WB
    36 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 50% glycerol
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for mCherry Antibody (1C51)

  • DSRED
  • red fluorescent protein mCherry
  • Red Fluoroscent Protein

Background

mCherry is a monomeric red fluorescent protein (mRFP) belonging to the mFruits family which is brighter and more photostable compared to the first-generation mRFP1, making them ideal for fluorescence microscopy (1). mCherry has an excitation maximum at 587 nm and an emission maximum at 610 nm. mCherry protein was derived from DsRed, a red fluorescent protein from the coral Discosoma (disc anemone) (2). The red chromophore of DsRed has a similar topology to GFP, the green fluorescent protein isolated from the jellyfish Aequorea Victoria, but has extended pi-electron conjugation resulting in red-shifted absorbance and emission (3). mCherry is 236 amino acids (aa) in length with a theoretical molecular weight of 28 kDa and has a crystal structure with the chromophore forming a central helix shielded within an eleven-stranded beta-barrel (3).

mCherry can be used as a long-wavelength hetero-FRET (fluorescence resonance energy transfer) acceptor and probe for homoFRET experiments given its high peak molar absorptivity, folding efficiency, and superior spectral properties (4). Additionally, because mCherry does not interfere with other plasmids or alter the growth of Legionella species during intracellular growth, it can be used for constitutive gene expression in a variety of gram-negative bacterial species (5). For example, a plasmid developed to constitutively express mCherry under the Ptac promoter has been used in several Legionella species including L. pneumophila, the causative agent of Legionnaires' disease (5).

References

1. Shaner, N. C., Steinbach, P. A., & Tsien, R. Y. (2005). A guide to choosing fluorescent proteins. Nature Methods, 2(12), 905-909. doi:10.1038/nmeth819

2. Bevis, B. J., & Glick, B. S. (2002). Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed). Nature Biotechnology, 20(1), 83-87. https://doi.org/10.1038/nbt0102-83

3. Wall, M. A., Socolich, M., & Ranganathan, R. (2000). The structural basis for red fluorescence in the tetrameric GFP homolog DsRed. Nature Structural Biology, 7(12), 1133-1138. https://doi.org/10.1038/81992

4. Akrap, N., Seidel, T., & Barisas, B. G. (2010). Forster distances for fluorescence resonant energy transfer between mCherry and other visible fluorescent proteins. Analytical Biochemistry, 402(1), 105-106. https://doi.org/10.1016/j.ab.2010.03.026

5. Gebhardt, M. J., Jacobson, R. K., & Shuman, H. A. (2017). Seeing red; the development of pON.mCherry, a broad-host range constitutive expression plasmid for Gram-negative bacteria. Plos One, 12(3), e0173116. https://doi.org/10.1371/journal.pone.0173116

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for mCherry Antibody (NBP1-96752)(57)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Drosophila, Insect, Zebrafish.

We have publications tested in 7 applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB.


Filter By Application
Flow
(1)
ICC/IF
(5)
IHC
(4)
IHC-P
(2)
IP
(2)
KO
(1)
WB
(36)
All Applications
Filter By Species
Human
(7)
Mouse
(2)
Rat
(1)
Drosophila
(1)
Insect
(1)
Zebrafish
(3)
All Species
Showing Publications 1 - 10 of 57. Show All 57 Publications.
Publications using NBP1-96752 Applications Species
Belyaeva V, Wachner S, Gyoergy A et al. Fos regulates macrophage infiltration against surrounding tissue resistance by a cortical actin-based mechanism in Drosophila PLoS biology Jan 1 2022 [PMID: 34990456] (WB) WB
Seervai RNH, Jangid RK, Karki M et al. The Huntingtin-interacting protein SETD2/HYPB is an actin lysine methyltransferase Sci Adv Oct 1 2020 [PMID: 33008892] (IP, WB) IP, WB
Rebman JK Cellular and Molecular Mechanisms of Collective Migration in Facial Branchiomotor Neurons Thesis (IHC, Zebrafish) IHC Zebrafish
Israel S, Drexler Hca, Fuellen G, Boiani M The COP9 signalosome subunit 3 is necessary for early embryo survival by way of a stable protein deposit in mouse oocytes Molecular human reproduction Jul 15 2021 [PMID: 34264319]
Glykofrydis F, Cachat E, Berzanskyte I et al. Bioengineering Self-Organizing Signaling Centers to Control Embryoid Body Pattern Elaboration ACS synthetic biology May 21 2021 [PMID: 34019395]
Jacomin A, Petridi S, Di Monaco M, et al. Self-Regulation of Autophagy Genes Expression by Atg8a and Its Interacting Partners YL-1, Sequoia and Sir2 In Drosophila SSRN Journal Dec 5 2019
Finke M, Brecht D, Stifel J et al. Efficient splicing-based RNA regulators for tetracycline-inducible gene expression in human cell culture and C. elegans Nucleic acids research Apr 24 2021 [PMID: 33893804]
Sobu Y, Wawro PS, Dhekne HS, Pfeffer SR Pathogenic LRRK2 regulates ciliation probability upstream of Tau Tubulin kinase 2 BioRxiv Jan 1 2020
Peterson J, Li S, Kaltenbrun E et al. Expression of transgenes enriched in rare codons is enhanced by the MAPK pathway Scientific reports Dec 17 2020 [PMID: 33335127] (WB, IP, Flow) WB, IP, Flow
Singh SR, Meyer-Jens M, Alizoti E et al. A high-throughput screening identifies ZNF418 as a novel regulator of the ubiquitin-proteasome system and autophagy-lysosomal pathway Autophagy Dec 27 2020 [PMID: 33249983] (WB) WB
Show All 57 Publications.

Reviews for mCherry Antibody (NBP1-96752) (0)

There are no reviews for mCherry Antibody (NBP1-96752). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for mCherry Antibody (NBP1-96752). (Showing 1 - 6 of 6 FAQs).

  1. Does this antibody cross-react with GFP epitopes? As I would like to use both GFP and mCherry antibodies during histochemistry I would not like them to cross-react.
    • mCherry and GFP share just 29% sequence similarity, so this antibody is not predicted to cross-react to GFP and has never shown any ability to detect GFP in testing.
  2. Would this antibody detect DsRed?
    • NBP1-45840, NBP1-97373 and NBP1-97371 will all recognize DsRed. I have no information about its cross-reactivity of DsRed with NBP1-96752.
  3. Do you have any data on the use of NBP1-96752 for immunohistochemistry?
    • At this time we do not have any date on the use of NBP1-96752 in Immunohistochemistry. If you would be interested in testing this antibody, I would invite you to take a look at our Innovator's Reward Program.
  4. Regarding RFP Antibody (NBP1-97373). Does it recognize dTomato?
    • I am sorry, but product NBP1-97373 has been discontinued. However, I have now received confirmation from the lab that mCherry Antibody (1C51) NBP1-96752 does indeed bind to dTomato, and so we would recommend this antibody for you.
  5. Regarding the mCherry antibody (NBP1-96752) I would like to know if it also detects RFP
    • We have not tested our mCherry antibody with catalogue number NBP1-96752 against RFP, however since the two proteins share a high degree of sequence homology the antibody is likely to recognise RFP. As we have not performed this testing in house however, we cannot guarantee that NBP1-96752 will or will not cross-react with RFP.
  6. I'm looking for an mCherry antibody to use for WB and got a paper that references your NBP1-96752 and was thinking to buy it. But then I saw your WB picture (image 6) in your webpage, and I don't really get why the antibody does not see only one band (about 28kDA) for the mCherry. What are these "different processed forms" supposed to be?
    • mCherry differs from other GFP-derived proteins by maturing extremely rapidly, and is highly photostable and resists photobleaching. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910338/).In short this protein does not naturally occur in the higher mammals, and this is relevant to answer your concern. Since not present in the higher mammals, the only way for our lab to easily assess whether NBP1-96752 was specific to mCharry protein was using transiently transfection of its gene (in pFin-EF1-mCherry vector) into the host of interests, in this case HEK293 cells. Therefore, the crude extract of HEk293 cells transfected with the gene (lane + on the posted WB image) could reveal the mCherry species by the antibody, but an equal amount of protein extract from untransfected HEK293 cells (lane -) could not.In these experiments, it was not the endogenous protein was detected. The transiently expressed protein may be associated with many detection artifacts, namely, degradation easier, pre-maturation termination of transcription/translation, aggression of protein due to over expression of the proteins in situ, and many others. All of these might be contributing to the detection of the mCherry bands, as those see on the image.Since this antibody have been cited in many publications (9), 8 of which were using the antibody in WB, we are confident that the quality of NBP1-96752 is good. In fact we 100% guarantee it to produce the positive results in WB. Or we will refund or free replacement of any primary antibody with the similar price.

Secondary Antibodies

 

Isotype Controls

Additional mCherry Products

Array NBP1-96752

Research Areas for mCherry Antibody (NBP1-96752)

Find related products by research area.

Blogs on mCherry.

Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents
Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera...  Read full blog post.

Autophagy and RAS signaling: Clinical implications
By Christina Towers, PhD The cellular recycling process known as autophagy is currently being targeted in over 60 clinical trials focused on treating different types of cancer1. To date, the only autophagy-targeted ...  Read full blog post.

Autophagic flux: Is p62 a good indicator?
By Christina Towers, PhD Is p62 a good indicator of autophagic flux? The short answer: Yes … but … SQSTM1 encodes the cargo adaptor protein, p62, which interacts with autophagic substrates and delivers them to aut...  Read full blog post.

Make each cell count: How to assess autophagy using flow cytometry
Kristy R. Howell, PhDThe cellular recycling process known as autophagy may be induced by a variety of conditions including reduced nutrient availability, serum starvation and pharmacological agents (e.g., Rapamyci...  Read full blog post.

How to visualize autophagy by microscopy
By Christina Towers, PhD Autophagy is a recycling process that relies on the formation of a unique organelle termed an autophagosome. An elegant way to monitor autophagy is through various microscopy techniques to...  Read full blog post.

Best way to quantitatively measure Autophagic Flux
By Christina Towers, PhD Autophagy is a stress-induced cellular recycling process that plays an important physiological role in many diseases. It is induced by a variety of stimuli, both intracellular and extracel...  Read full blog post.

Animal Models to Study Autophagy
By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the...  Read full blog post.

Application Focus: I see an increase in LC3, now what?
 By Christina Towers, PhD.  Autophagy is highly conserved and tightly regulated process that all cell types use to recycle nutrients, particularly in the instance of stress1. As a result, even sm...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our mCherry Antibody (1C51) and receive a gift card or discount.