Immunohistochemistry: mCherry Antibody (1C51) [NBP1-96752] - Cadherin-2 is required cell autonomously for caudal migration of FBMNs. (A-I) Whole-mount immunocytochemistry showing dorsal views of Tg (isl1:GFP) (A-C) and ...read more
Western Blot: mCherry Antibody (1C51) [NBP1-96752] - Analysis of HEK293 cell lysates and recombinant protein solutions using mCherry antibody, dilution 1:1,000 (Green).  protein standard,  HEK293,  HEK293 cells ...read more
Western Blot: mCherry Antibody (1C51) [NBP1-96752] - Fluorescent signals and immunoblots of the dual fluorescence reporter of cup-5 32-UTR in WT worms and mir-83(-) mutants at day 1 of adulthood. Quantification is from ...read more
Western Blot: mCherry Antibody (1C51) [NBP1-96752] - Threonine 276 is required for the intramolecular interaction, and for inhibition of membrane binding. Mutation of threonine 276 to aspartic acid promotes the ...read more
Immunocytochemistry/ Immunofluorescence: mCherry Antibody (1C51) [NBP1-96752] - HEK293 cells transfected with mCherry and visualized in red. The cells were stained with NBP1-96752 in the green channel, and visualized ...read more
Western Blot: mCherry Antibody (1C51) [NBP1-96752] - WB assay of the crude extract of HEK293 cells transfected with pFin-EF1-mCherry vector (lane +) and an equal amount of protein extract from untransfected HEK293 ...read more
Immunohistochemistry-Frozen: mCherry Antibody (1C51) [NBP1-96752] - Mouse Bone Marrow Sections (Femur). Fixed-frozen and decalcified. tdTomato reporter transgenic mice. tdTomato in hematopoietic cells were detected by ...read more
WB, Flow, ICC/IF, IHC, IP, KO, Single-Cell Western
Use in Flow reported in scientific literature (PMID:33335127). Use in IHC and IHC-P reported in scientific literature (PMID: 27396338 and 27716840 respectively). mCherry antibody validated for IHC-Frozen from a verified customer review. Use in Immunoprecipitation reported in scientific literature (PMID: 33008892). Use in Knockout Validation was reported in scientific literature (PMID: 32547960).
27 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 3 Reviews rated 4.3 using NBP1-96752 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
50% PBS, 50% glycerol
5mM Sodium Azide
Protein G purified
Alternate Names for mCherry Antibody (1C51)
red fluorescent protein mCherry
Red Fluoroscent Protein
mCherry is a monomeric red fluorescent protein (mRFP) belonging to the mFruits family which is brighter and more photostable compared to the first-generation mRFP1, making them ideal for fluorescence microscopy (1). mCherry has an excitation maximum at 587 nm and an emission maximum at 610 nm. mCherry protein was derived from DsRed, a red fluorescent protein from the coral Discosoma (disc anemone) (2). The red chromophore of DsRed has a similar topology to GFP, the green fluorescent protein isolated from the jellyfish Aequorea Victoria, but has extended pi-electron conjugation resulting in red-shifted absorbance and emission (3). mCherry is 236 amino acids (aa) in length with a theoretical molecular weight of 28 kDa and has a crystal structure with the chromophore forming a central helix shielded within an eleven-stranded beta-barrel (3).
mCherry can be used as a long-wavelength hetero-FRET (fluorescence resonance energy transfer) acceptor and probe for homoFRET experiments given its high peak molar absorptivity, folding efficiency, and superior spectral properties (4). Additionally, because mCherry does not interfere with other plasmids or alter the growth of Legionella species during intracellular growth, it can be used for constitutive gene expression in a variety of gram-negative bacterial species (5). For example, a plasmid developed to constitutively express mCherry under the Ptac promoter has been used in several Legionella species including L. pneumophila, the causative agent of Legionnaires' disease (5).
1. Shaner, N. C., Steinbach, P. A., & Tsien, R. Y. (2005). A guide to choosing fluorescent proteins. Nature Methods, 2(12), 905-909. doi:10.1038/nmeth819
2. Bevis, B. J., & Glick, B. S. (2002). Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed). Nature Biotechnology, 20(1), 83-87. https://doi.org/10.1038/nbt0102-83
3. Wall, M. A., Socolich, M., & Ranganathan, R. (2000). The structural basis for red fluorescence in the tetrameric GFP homolog DsRed. Nature Structural Biology, 7(12), 1133-1138. https://doi.org/10.1038/81992
4. Akrap, N., Seidel, T., & Barisas, B. G. (2010). Forster distances for fluorescence resonant energy transfer between mCherry and other visible fluorescent proteins. Analytical Biochemistry, 402(1), 105-106. https://doi.org/10.1016/j.ab.2010.03.026
5. Gebhardt, M. J., Jacobson, R. K., & Shuman, H. A. (2017). Seeing red; the development of pON.mCherry, a broad-host range constitutive expression plasmid for Gram-negative bacteria. Plos One, 12(3), e0173116. https://doi.org/10.1371/journal.pone.0173116
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for mCherry Antibody (NBP1-96752)(75)
We have publications tested in 6 confirmed species: Human, Mouse, Rat, Drosophila, Insect, Zebrafish.
We have publications tested in 9 applications: B/N, FLOW, ICC/IF, IF/IHC, IHC-Fr, IHC-P, IP, KO, WB.
mCherry under a CMV promoter was tested by standard western blot at a 1:1000 dilution in 5% milk. Blot showed good bands with no non-specific bands. Note: This antibody does not detect TdTomato by Western Blot.
FAQs for mCherry Antibody (NBP1-96752). (Showing 1 - 6 of 6 FAQs).
Does this antibody cross-react with GFP epitopes? As I would like to use both GFP and mCherry antibodies during histochemistry I would not like them to cross-react.
mCherry and GFP share just 29% sequence similarity, so this antibody is not predicted to cross-react to GFP and has never shown any ability to detect GFP in testing.
Would this antibody detect DsRed?
NBP1-45840, NBP1-97373 and NBP1-97371 will all recognize DsRed. I have no information about its cross-reactivity of DsRed with NBP1-96752.
Do you have any data on the use of NBP1-96752 for immunohistochemistry?
At this time we do not have any date on the use of NBP1-96752 in Immunohistochemistry. If you would be interested in testing this antibody, I would invite you to take a look at our Innovator's Reward Program.
Regarding RFP Antibody (NBP1-97373). Does it recognize dTomato?
I am sorry, but product NBP1-97373 has been discontinued. However, I have now received confirmation from the lab that mCherry Antibody (1C51) NBP1-96752 does indeed bind to dTomato, and so we would recommend this antibody for you.
Regarding the mCherry antibody (NBP1-96752) I would like to know if it also detects RFP
We have not tested our mCherry antibody with catalogue number NBP1-96752 against RFP, however since the two proteins share a high degree of sequence homology the antibody is likely to recognise RFP. As we have not performed this testing in house however, we cannot guarantee that NBP1-96752 will or will not cross-react with RFP.
I'm looking for an mCherry antibody to use for WB and got a paper that references your NBP1-96752 and was thinking to buy it. But then I saw your WB picture (image 6) in your webpage, and I don't really get why the antibody does not see only one band (about 28kDA) for the mCherry. What are these "different processed forms" supposed to be?
mCherry differs from other GFP-derived proteins by maturing extremely rapidly, and is highly photostable and resists photobleaching. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910338/).In short this protein does not naturally occur in the higher mammals, and this is relevant to answer your concern. Since not present in the higher mammals, the only way for our lab to easily assess whether NBP1-96752 was specific to mCharry protein was using transiently transfection of its gene (in pFin-EF1-mCherry vector) into the host of interests, in this case HEK293 cells. Therefore, the crude extract of HEk293 cells transfected with the gene (lane + on the posted WB image) could reveal the mCherry species by the antibody, but an equal amount of protein extract from untransfected HEK293 cells (lane -) could not.In these experiments, it was not the endogenous protein was detected. The transiently expressed protein may be associated with many detection artifacts, namely, degradation easier, pre-maturation termination of transcription/translation, aggression of protein due to over expression of the proteins in situ, and many others. All of these might be contributing to the detection of the mCherry bands, as those see on the image.Since this antibody have been cited in many publications (9), 8 of which were using the antibody in WB, we are confident that the quality of NBP1-96752 is good. In fact we 100% guarantee it to produce the positive results in WB. Or we will refund or free replacement of any primary antibody with the similar price.
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