mCherry Antibody (1C51) [FITC]

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Product Details

Summary
Reactivity AllSpecies Glossary
Applications IHC
Clone
1C51
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

mCherry Antibody (1C51) [FITC] Summary

Immunogen
This antibody is raised against recombinant full-length mCherry with His tag purified from E. coli.
Specificity
Does not cross react with GFP.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
Application Notes
This mCherry (1C51) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 28 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Optimal dilution of this antibody should be experimentally determined.
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for mCherry Antibody (1C51) [FITC]

  • DSRED
  • red fluorescent protein mCherry
  • Red Fluoroscent Protein

Background

mCherry represents an engineered derivative of a family of proteins initially isolated from a group of sea organisms called Cnidarians (jelly fish, sea anemones and corals). The prototype for these fluorescent proteins is GFP which was originally isolated from jellyfish Aequoria Victoria and when expressed in-vitro or in-vivo using its gene, GFP protein fold correctly with retention of its capability to deliver strong fluorescence. Engineered forms of GFP and its relatives have been developed to monitor several biological proresses such as Ca2+ levels, protease activation and a variety of other processes in real time. mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of Discosoma species. DsRed is similar in size and other properties to GFP, but, produces a red rather than a green fluoresnecce and its engineered monomeric form has several applications including FRET (Forster Resonance Energy Transfer). mCherry was engineered with further multiple cycles of mutation, directed modification and evolutionary selection, and it is also a monomeric form with an excitation maximum at 587 nm and an emission maximum at 610 nm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 350 Labeled NBP1-96752UV
DyLight 405 Labeled NBP1-96752V
DyLight 488 Labeled NBP1-96752G
DyLight 680 Labeled NBP1-96752FR
DyLight 755 Labeled NBP1-96752IR

Additional mCherry Products

mCherry NBP1-96752F

Research Areas for mCherry Antibody (NBP1-96752F)

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