Western Blot: mCherry Antibody [NBP2-25158] - Blot of Hek293 cells transfected with pFin-EF1-mCherry vector, courtesy of the Semple-Rowland lab at the University of Florida. There is a strong clean band at about 30kDa. ...read more
Immunocytochemistry/ Immunofluorescence: mCherry Antibody [NBP2-25158] - HeK293 cells transfected in the same way and viewed in the confocal microscope. Most Hek293 cells are not transfected so only the nucleus of these ...read more
WB, ICC/IF, IHC
Please see the vial label for concentration. If unlisted please contact technical services.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
10mM Sodium Azide
Ammonium sulfate precipitation
Western Blot 1:2000
This mCherry antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~30 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry reported in scientific literature (PMID 27396338).
30 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
mCherry represents an engineered derivative of a family of proteins initially isolated from a group of sea organisms called Cnidarians (jelly fish, sea anemones and corals). The prototype for these fluorescent proteins is GFP which was originally isolated from jellyfish Aequoria Victoria and when expressed in-vitro or in-vivo using its gene, GFP protein fold correctly with retention of its capability to deliver strong fluorescence. Engineered forms of GFP and its relatives have been developed to monitor several biological proresses such as Ca2+ levels, protease activation and a variety of other processes in real time. mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of Discosoma species. DsRed is similar in size and other properties to GFP, but, produces a red rather than a green fluoresnecce and its engineered monomeric form has several applications including FRET (Forster Resonance Energy Transfer). mCherry was engineered with further multiple cycles of mutation, directed modification and evolutionary selection, and it is also a monomeric form with an excitation maximum at 587 nm and an emission maximum at 610 nm.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for mCherry Antibody (NBP2-25158)(2)
We have publications tested in 2 applications: IHC, IHC-P.