Luciferase Antibody (Luci 21 1-107)

Images

 
Western Blot: Luciferase Antibody (Luci 21 1-107) [NB600-307] - WB analysis of NB600-307 on Luciferase Protein (NB810-74573)
Immunohistochemistry: Luciferase Antibody (Luci 21 1-107) [NB600-307] - Detection of Luciferase expression in CD34+ cells by Immunohistochemistry. Cytospin slides prepared from transduced CD34+ cells after 3 days of ...read more

Product Details

Summary
Reactivity PpSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
Luci 21 1-107
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Luciferase Antibody (Luci 21 1-107) Summary

Immunogen
Luciferase protein from Photinus pyralis (North American firefly). [UniProt# P08659]
Localization
Peroxisome
Specificity
Specific for Luciferase, recognizing a peptide consisting of the first 258 amino acids. Further epitope mapping has not been done at this time.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:200
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:1000
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
Application Notes
Western blot has been tested with Drosophila embryos. Purified Luciferase protein and Luciferase expressed in Drosophila adult co-migrate on Western blots with a band seen at ~61 kDa, representing Luciferase. Flow Cytometry has been reported in a customer review. Use in Immunohistochemistry-Frozen was reported in scientific literature (PMID: 31069316).
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB600-307 in the following applications:

Publications
Read Publications using
NB600-307 in the following applications:

Reactivity Notes

Photinus pyralis (North American firefly). Luciferase recombinant reactivity in Mouse and Rat reported by a customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Luciferase Antibody (Luci 21 1-107)

  • ec 1.13.12.7
  • Fluc
  • Luciferase
  • luciferin 4 monooxygenase

Background

Luciferase is a luminescent enzyme, mostly known for its role as a reporter protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Luciferase Antibody (NB600-307)(17)

We have publications tested in 3 confirmed species: Mouse, Rat, Firefly.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(4)
IHC
(2)
IHC-Fr
(1)
IHC-P
(2)
WB
(6)
All Applications
Filter By Species
Mouse
(4)
Rat
(1)
Firefly
(6)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB600-307 Applications Species
Chitwood C, Dietzsch C, Jacobs G et al. Breast tumor cell hybrids form spontaneously in vivo and contribute to breast tumor metastases APL Bioengineering Sep 1 2018 [PMID: 31069316] (IHC-Fr, Mouse) IHC-Fr Mouse
Shteingauz A, Porat Y, Voloshin T et al. AMPK-dependent autophagy upregulation serves as a survival mechanism in response to Tumor Treating Fields (TTFields) Cell Death Dis Oct 19 2018 [PMID: 30341282] (ICC/IF, WB, IHC) ICC/IF, WB, IHC
Galisova A, Herynek V, Swider E et al. A Trimodal Imaging Platform for Tracking Viable Transplanted Pancreatic Islets In Vivo: F-19 MR, Fluorescence, and Bioluminescence Imaging. Mol Imaging Biol Aug 30 2018 [PMID: 30167995] (IHC-P, Firefly) IHC-P Firefly
Cunningham D, Zhang Q, Liu S et al. Interleukin-17 promotes metastasis in an immunocompetent orthotopic mouse model of prostate cancer. Am J Clin Exp Urol. Jun 15 2018 [PMID: 30038943] (IHC-P, Mouse) IHC-P Mouse
Sayed D, Yang Z, He M et al. Acute Targeting of General Transcription Factor IIB Restricts Cardiac Hypertrophy via Selective Inhibition of Gene Transcription. Circ Heart Fail. 2014 Nov 14 [PMID: 25398966] (WB, Mouse, Rat) WB Mouse, Rat
Wang K, Liu S, Wang J et al. Transcriptional regulation of human USP24 gene expression by NF-kappa B. J Neurochem. 2013 Nov 29 [PMID: 24286619] (WB) WB
Schmeisser MJ, Baumann B, Johannsen S et al. IkB Kinase/Nuclear Factor ?B-Dependent Insulin-Like Growth Factor 2 (Igf2) Expression Regulates Synapse Formation and Spine Maturation via Igf2 Receptor Signaling. J Neurosci;32(16):5688-703. 2012 Apr 18. [PMID: 22514330] (ICC/IF, Mouse) ICC/IF Mouse
Li JF, Bush J, Xiong Y et al. Large-scale protein-protein interaction analysis in Arabidopsis mesophyll protoplasts by split firefly luciferase complementation. PLoS One 2011 [PMID: 22096563] (WB, Firefly) WB Firefly
Iijima K, Zhao L, Shenton C, Iijima-Ando K. Regulation of energy stores and feeding by neuronal and peripheral CREB activity in Drosophila. PLoS One;4(12):e8498. 2009 Dec 30. [PMID: 20041126] (WB) WB
Chinta, SJ et al. Inducible alterations of glutathione levels in adult dopaminergic midbrain neurons result in nigrostriatal degeneration. J Neurosci;27(51):13997-4006. 2007 Dec 19. [PMID: 18094238]
Show All 17 Publications.

Reviews for Luciferase Antibody (NB600-307) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 review tested in 1 species: Rat.

Reviews using NB600-307:
Filter by Applications
Flow
(1)
WB
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry Luciferase NB600-307
Enlarge
reviewed by:
Anonymous
Flow 12/05/2012
View

Summary

ApplicationFlow Cytometry
LotA
Special ApplicationsTested 4T1 mouse mammary carcinoma cell line transfected with luciferase
FileView PDF
 
reviewed by:
Anonymous
WB Rat 04/22/2010
View

Summary

ApplicationWestern Blot
Sample Testedcardiomyocytes cell lysate, Sample Amount: 15ug
SpeciesRat
LotA

Blocking

Blocking DetailsBlocking Buffer: 5% BSA, Blocking Time: 30 minutes, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Dilution Buffer: 5% BSA, Incubation Time: 2hr, Incubation Temp: RT

Secondary Antibody

Secondary DescriptionSecondary Ab: goat anti-mouse igG (H+L)-HRP Conjugate, Secondary Ab Dilution Ratio: 1:5000
Secondary Manufacturer Cat#170-6516

Details

Detection NotesDetection Method: ECL, Exposure Time:5sec, Neg Ctrl: cell w/o luciferase t/t, Specific Bands:64kDa, Non-Specific Bands:None

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Luciferase Antibody (NB600-307). (Showing 1 - 1 of 1 FAQs).

  1. Hello. Do you have a specific protocol for use of this antibody in FACS analysis? Luciferase Antibody (Luci 21 1-107) 0.1ml. It is uncojugated, so I am wondering if there is a specific protocol for labeling, etc. Thank you
    • The flow protocol used with this antibody NB600-307 was our general flow protocol. Since this antibody is unconjugated, you would want to use a conjugated mouse IgG secondary with this product in order to detect the antibody. We do also have this specific clone available in a directly conjugated PE form (NB600-307PE). If you are interested, I can inquire about a custom conjugate of another fluor or dye if you like. The other option would be for you to directly conjugate the antibody yourself. We do sell several conjugation kits that are very easy to use.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-307AF350
Alexa Fluor 405 NB600-307AF405
Alexa Fluor 488 NB600-307AF488
Alexa Fluor 532 NB600-307AF532
Alexa Fluor 594 NB600-307AF594
Alexa Fluor 647 NB600-307AF647
Alexa Fluor 700 NB600-307AF700
Alexa Fluor 750 NB600-307AF750
Allophycocyanin NB600-307APC
Allophycocyanin/Cy7 NB600-307APCCY7
Biotin NB600-307B
DyLight 350 NB600-307UV
DyLight 405 NB600-307V
DyLight 488 NB600-307G
DyLight 550 NB600-307R
DyLight 594 NB600-307DL594
DyLight 650 NB600-307C
DyLight 680 NB600-307FR
DyLight 755 NB600-307IR
FITC NB600-307F
HRP NB600-307H
Janelia Fluor 549 NB600-307JF549
Janelia Fluor 646 NB600-307JF646
PE NB600-307PE
PE/Atto594 NB600-307PEATT594
PE/Cy5.5 NB600-307PECY55
PE/Cy7 NB600-307PECY7
PerCP NB600-307PCP

Additional Luciferase Products

Research Areas for Luciferase Antibody (NB600-307)

Find related products by research area.

Blogs on Luciferase.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

Luciferase: Shining a Light to See Inside Living Animal Models
The luciferase reporter is a valuable tool for research into physiology and disease. Light emitted from luciferase enables the monitoring of xenografted tumors, specific cell types, gene expression and pathogens within live animals over time using bio...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Anonymous
12/05/2012
Application: Flow
Species:

 
Anonymous
04/22/2010
Application: WB
Species: Rat

Bioinformatics

Uniprot