Western Blot: Luciferase Antibody [NB100-1677] - Lane 1: Luciferase, 50ng.Luciferase antibody at 1:1000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Block: ...read more
Immunocytochemistry/ Immunofluorescence: Luciferase Antibody [NB100-1677] - Human tumor xenograft expressing firefly luciferase intracranially injected into a mouse brain. Image provided via product review by verified ...read more
Immunohistochemistry-Paraffin: Luciferase Antibody [NB100-1677] - Luciferase expression in mouse brain tissue bearing human glioma cells expressing fire fly luciferase. Image from verified customer review.
Western Blot: Luciferase Antibody [NB100-1677]
Immunocytochemistry/ Immunofluorescence: Luciferase Antibody [NB100-1677] - Luciferase in 247Nluc+ cells. Image provided via product review by verified customer.
Immunohistochemistry-Paraffin: Luciferase Antibody [NB100-1677] - NIH3T3 cells. Cells (25,000/well) transfected with pLuc plasmid were stained with 20ug/ml NB100-1677 and 1:200 dilution of donkey anti-goat IgG-FITC ...read more
Immunohistochemistry-Paraffin: Luciferase Antibody [NB100-1677] - Luciferase immunostaining was analyzed in tibias from ovx ERE-luciferase mice taken 24 h after a 17-E2 injection. Positive luciferase staining was ...read more
This Luciferase antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Native protein from Photinus pyralis (Firefly).
Luciferase, no reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
Ion exchange chromatography
Test in a species/application not listed above to receive a full credit towards a future purchase.
Reporter assay reactivity reported in human in the scientific literature (PMID: 25524705). Reactivity in a mouse cell line with a reporter vector.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
Ion exchange chromatography
Alternate Names for Luciferase Antibody
luciferin 4 monooxygenase
Luciferase is a reporter enzyme that catalyzes a bioluminescent reaction involving the enzyme, Mg2+ and ATP. This reaction produces a brief flash of light that can be detected by a luminometer. The amount of light emitted is directly proportional to the activity of luciferase, making luciferase an excellent tool for studying gene expression.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for Luciferase Antibody (NB100-1677)(9)
We have publications tested in 3 confirmed species: Human, Mouse, Firefly.
We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, WB.
FAQs for Luciferase Antibody (NB100-1677). (Showing 1 - 2 of 2 FAQs).
One of our customers would like additional information about your goat anti-luciferase (catalog #NB100-1677). They want to use this antibody for immunohistochemistry of paraffin-embedded sections, and would like to know if you recommend an antigen retrieval step? If so, which one is best?
For IHC-P with NB100-1677, we would recommend 10 mm citrate buffer (pH 6.0) based heat induced antigen retrieval at 95-99 C.
Hello, can I use this antibody (NB100-1677) for immunohistochemistry on rat frozen brain sections?
This antibody will work in IHC on frozen and paraffin embedded tissues, provided that they express Firefly Luciferase from Photinus pyralis.
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