Luciferase Antibody


Immunocytochemistry/ Immunofluorescence: Luciferase Antibody [NB100-1677] - Human tumor xenograft expressing firefly luciferase intracranially injected into a mouse brain. Image provided via product review by verified more
Western Blot: Luciferase Antibody [NB100-1677] - Lane 1: Luciferase, 50ng.Luciferase antibody at 1:1000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Block: more
Immunocytochemistry/ Immunofluorescence: Luciferase Antibody [NB100-1677] - In vitro expression of Luciferase by 247Nluc+ recombinant strain of Alcelaphine herpesvirus 1 (AIHV-1). Epifluoresence expression of Luciferase more
Immunohistochemistry-Paraffin: Luciferase Antibody [NB100-1677] - Analysis of human breast tumor xenograft expressing firefly luciferase subcutaneously injected into a mouse using Luciferase antibody. Primary antibody more
Immunohistochemistry-Paraffin: Luciferase Antibody [NB100-1677] - NIH3T3 cells. Cells (25,000/well) transfected with pLuc plasmid were stained with 20ug/ml NB100-1677 and 1:200 dilution of donkey anti-goat IgG-FITC more
Immunohistochemistry-Paraffin: Luciferase Antibody [NB100-1677] - Luciferase immunostaining was analyzed in tibias from ovx ERE-luciferase mice taken 24 h after a 17-E2 injection. Positive luciferase staining was more
Immunohistochemistry-Paraffin: Luciferase Antibody [NB100-1677] - Luciferase expression in mouse brain tissue bearing human glioma cells expressing fire fly luciferase. Image from verified customer review.

Product Details

Reactivity Hu, PpSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P

Order Details

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Luciferase Antibody Summary

Luciferase [Photinus pyralis (Firefly)]
No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA 1:200-1:1000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Frozen 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Western Blot 1:1000-1:5000
Application Notes
This antibody has been tested in Western Blot, IHC, and ELISA. Expect a band ~60kDa in appropriate cell lysates. Although not tested, this antibody would be useful in immunofluorescence, immunoprecipitation, immunocytochemistry, and most immunological methods requiring high titers and specificity.
Read Publications using
NB100-1677 in the following applications:

Reactivity Notes

No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.

Packaging, Storage & Formulations

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide


This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)]

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Luciferase Antibody

  • ec
  • firefly luciferase
  • Fluc
  • LuC
  • Luciferase
  • luciferin 4 monooxygenase
  • Luciferin 4-monooxygenase


Luciferase is a generic term for a group of oxidative enzymes used in bioluminescence. Firefly (Photinus pyralis) and bacterial luciferase enzymes are commonly used in assay systems such as cell viability assays, reporter gene assays, and for in vivo imaging. Bacterial luciferases are flavoenzymes composed of two subunits each encoded by the luxA and luxB genes, while the firefly luciferase is a single polypeptide specified by the luc gene (1). Firefly luciferase (theoretical molecular weight: 61 kDa) oxidizes the substrate luciferin to oxyluciferin in a bioluminescent reaction requiring Mg2+ and ATP (2,3). This reaction produces a flash of yellow-green light with an emission peak around 560nm that can be detected by a luminometer (3). Firefly luciferase has become one of the more widely used reporter proteins and is an excellent tool for the study of gene expression, given that the amount of light emitted is directly proportional to luciferase activity (4).

The luciferase assay is fast and sensitive, differentiating itself from the CAT (chloramphenicol acetyltransferase) assay because it does not require a radioactive substrate.


1. Eun, H. (1996). Marker/Reporter enzymes. Enzymology Primer for Recombinant DNA Technology, 567-645. doi:10.1016/b978-012243740-3/50011-9

2. McNabb, D. S., Reed, R., & Marciniak, R. A. (2005). Dual luciferase assay system for rapid assessment of gene expression in Saccharomyces cerevisiae. Eukaryotic Cell, 4(9), 1539-1549. doi:10.1128/ec.4.9.1539-1549.2005

3. Fraga, H. (2008). Firefly luminescence: A historical perspective and recent developments. Photochemical & Photobiological Sciences, 7(2), 146-158. doi:10.1039/b719181b

4. Younes, A., Lukyanenko, Y. O., Lyashkov, A. E., Lakatta, E. G., & Sollott, S. J. (2011). A bioluminescence method for direct measurement of phosphodiesterase activity. Analytical Biochemistry, 417(1), 36-40. doi:10.1016/j.ab.2011.05.036


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Luciferase Antibody (NB100-1677)(23)

We have publications tested in 1 confirmed species: Firefly.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-P, MiAr, WB.

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Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NB100-1677 Applications Species
Trieu KG, Tsai SY, Eberl M et al. Basal cell carcinomas acquire secondary mutations to overcome dormancy and progress from microscopic to macroscopic disease Cell reports 2022-05-03 [PMID: 35508126] (ICC/IF) ICC/IF
Lu X, Fong KW, Gritsina G et al. HOXB13 suppresses de novo lipogenesis through HDAC3-mediated epigenetic reprogramming in prostate cancer Nature genetics [PMID: 35468964] (MiAr) MiAr
Noffsinger B, Witter A, Sheybani N Et al. Technical choices significantly alter the adaptive immune response against immunocompetent murine gliomas in a model-dependent manner Journal of neuro-oncology 2021-08-25 [PMID: 34432197]
Gutierrez S, Eisenach Jc, Boada Md Seeding of breast cancer cell line (MDA-MB-231LUC+) to the mandible induces overexpression of substance P and CGRP throughout the trigeminal ganglion and widespread peripheral sensory neuropathy throughout all three of its divisions Molecular pain 2021-07-07 [PMID: 34229504] (IHC-Fr) IHC-Fr
Lazaro-Ibanez E, Faruqu FN, Saleh AF et al. Selection of Fluorescent, Bioluminescent, and Radioactive Tracers to Accurately Reflect Extracellular Vesicle Biodistribution in Vivo ACS Nano 2021-01-20 [PMID: 33470092] (WB) WB
Kang MH, van Lieshout LP, Xu L et al. A lung tropic AAV vector improves survival in a mouse model of surfactant B deficiency Nat Commun 2020-08-09 [PMID: 32764559] (ICC/IF) ICC/IF
Garg S, Wu C et al. Express ELISA for detection of mortalin. Biotechniques 2019-01-10 [PMID: 31502469] (ICC/IF) ICC/IF
Chen TH, Arra M, Mbalaviele G, Swarnkar G, Abu-Amer Y. Inflammatory Responses Reprogram T(REGS) Through Impairment of Neuropilin-1 Sci Rep 2019-07-20 [PMID: 31320680] (IHC-P) IHC-P
Ohta Y, Nomura E, Shang J, et al. Enhanced oxidative stress and the treatment by edaravone in mice model of amyotrophic lateral sclerosis J Neurosci Res 2018-12-20 [PMID: 30565312] (ICC/IF) ICC/IF
Singh RD, Hillestad ML, Livia C, et al. M(3)RNA Drives Targeted Gene Delivery in Acute Myocardial Infarction Tissue Eng Part A 2018-07-27 [PMID: 30047313] (ICC/IF) ICC/IF
Show All 23 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Luciferase Antibody (NB100-1677). (Showing 1 - 2 of 2 FAQs).

  1. One of our customers would like additional information about your goat anti-luciferase (catalog #NB100-1677). They want to use this antibody for immunohistochemistry of paraffin-embedded sections, and would like to know if you recommend an antigen retrieval step? If so, which one is best?
    • For IHC-P with NB100-1677, we would recommend 10 mm citrate buffer (pH 6.0) based heat induced antigen retrieval at 95-99 C.
  2. Hello, can I use this antibody (NB100-1677) for immunohistochemistry on rat frozen brain sections?
    • This antibody will work in IHC on frozen and paraffin embedded tissues, provided that they express Firefly Luciferase from Photinus pyralis.

Secondary Antibodies


Isotype Controls

Additional Luciferase Products

Research Areas for Luciferase Antibody (NB100-1677)

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Blogs on Luciferase.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

Luciferase: Shining a Light to See Inside Living Animal Models
The luciferase reporter is a valuable tool for research into physiology and disease. Light emitted from luciferase enables the monitoring of xenografted tumors, specific cell types, gene expression and pathogens within live animals over time using bio...  Read full blog post.

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