Luciferase Antibody

Images

 
Western Blot: Luciferase Antibody [NB100-1677] - Lane 1: Luciferase, 50ng.Luciferase antibody at 1:1000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Block: ...read more
Immunocytochemistry/ Immunofluorescence: Luciferase Antibody [NB100-1677] - Human tumor xenograft expressing firefly luciferase intracranially injected into a mouse brain. Image provided via product review by verified ...read more
Immunohistochemistry-Paraffin: Luciferase Antibody [NB100-1677] - Luciferase expression in mouse brain tissue bearing human glioma cells expressing fire fly luciferase. Image from verified customer review.
Western Blot: Luciferase Antibody [NB100-1677]
Immunocytochemistry/ Immunofluorescence: Luciferase Antibody [NB100-1677] - Luciferase in 247Nluc+ cells. Image provided via product review by verified customer.
Immunohistochemistry-Paraffin: Luciferase Antibody [NB100-1677] - NIH3T3 cells. Cells (25,000/well) transfected with pLuc plasmid were stained with 20ug/ml NB100-1677 and 1:200 dilution of donkey anti-goat IgG-FITC ...read more
Immunohistochemistry-Paraffin: Luciferase Antibody [NB100-1677] - Luciferase immunostaining was analyzed in tibias from ovx ERE-luciferase mice taken 24 h after a 17-E2 injection. Positive luciferase staining was ...read more

Product Details

Summary
Reactivity PpSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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Catalog# & Conjugate Size Price

Luciferase Antibody Summary

Description
This Luciferase antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Native protein from Photinus pyralis (Firefly).
Specificity
Luciferase, no reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:5000
  • ELISA 1:200 - 1:1000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Frozen 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
Reviewed Applications
Read 4 Reviews rated 4.8
using
NB100-1677 in the following applications:

Publications
Read Publications using
NB100-1677 in the following applications:

Reactivity Notes

Reporter assay reactivity reported in human in the scientific literature (PMID: 25524705). Reactivity in a mouse cell line with a reporter vector.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ion exchange chromatography

Alternate Names for Luciferase Antibody

  • ec 1.13.12.7
  • Fluc
  • Luciferase
  • luciferin 4 monooxygenase

Background

Luciferase is a reporter enzyme that catalyzes a bioluminescent reaction involving the enzyme, Mg2+ and ATP. This reaction produces a brief flash of light that can be detected by a luminometer. The amount of light emitted is directly proportional to the activity of luciferase, making luciferase an excellent tool for studying gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Luciferase Antibody (NB100-1677)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Firefly.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, WB.


Filter By Application
ICC/IF
(3)
IHC
(3)
IHC-Fr
(2)
WB
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
Firefly
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB100-1677 Applications Species
Luengo-Gil G, Calvo MI, Martin-Villar E et al. Antithrombin controls tumor migration, invasion and angiogenesis by inhibition of enteropeptidase. Sci Rep Jun 9 2016 [PMID: 27270881] (IHC-Fr) IHC-Fr
Vadrot N, Duband-Goulet I, Cabet E et al. The p.R482W substitution in A-type lamins deregulates SREBP1 activity in Dunnigan-type familial partial lipodystrophy. Hum. Mol. Genet. 2014 Dec 18 [PMID: 25524705] (ICC/IF, Human) ICC/IF Human
Sayed D, He M, Yang Z et al. Transcriptional Regulation Patterns Revealed by High Resolution Chromatin Immunoprecipitation during Cardiac Hypertrophy. J Biol Chem 2013 Jan 25 [PMID: 23229551] (WB, Firefly) WB Firefly
Ozdemir M, Attar A, Kuzu I. Regenerative treatment in spinal cord injury Curr Stem Cell Res Ther 2012 Sep 1 [PMID: 22849699]
Ozdemir M, Attar A, Kuzu I, Ayten M, Ozgencil E, Bozkurt M, Dalva K, Uckan D, K?l?c E, Sancak T, Kanpolat Y, Beksac M. Stem Cell Therapy in Spinal Cord Injury: In Vivo and Postmortem Tracking of Bone Marrow Mononuclear or Mesenchymal Stem Cells. Stem Cell Rev. 2012 May 3. [PMID: 22552878] (IHC-Fr, ICC/IF, Mouse) IHC-Fr, ICC/IF Mouse
Yang X, Balakrishnan I, Torok-Storb B, Pillai MM. Marrow Stromal Cell Infusion Rescues Hematopoiesis in Lethally Irradiated Mice despite Rapid Clearance after Infusion Adv Hematol 2012 [PMID: 22400029] (IHC, Mouse) IHC Mouse
Kawabata A, Baoum A, Ohta N et al. Intratracheal Administration of a Nanoparticle-Based Therapy with the Angiotensin II Type 2 Receptor Gene Attenuates Lung Cancer Growth. Cancer Res;72(8):2057-67. 2012 Apr 15. [PMID: 22389453] (IHC, Mouse) IHC Mouse
Dewals B, Myster F, Palmeira L et al. Ex vivo bioluminescence detection of alcelaphine herpesvirus 1 infection during malignant catarrhal fever J Virol 2011 Jul [PMID: 21593175] (ICC/IF) ICC/IF
Yang, G et al. Maturational differences in lung NF-alphaB activation their role in tolerance to hyproxia. J Clin Invest 114: 669-678. 2004 [PMID: 15343385] (IHC, Mouse) IHC Mouse

Reviews for Luciferase Antibody (NB100-1677) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Mouse, Other.
We have 4 reviews tested in 3 application: ICC/IF, IHC-P, ICC.

Reviews using NB100-1677:
Filter by Applications
ICC/IF
(1)
IHC-P
(2)
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
Other
(3)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry/Immunofluorescence Luciferase NB100-1677
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reviewed by:
Alexandra Ambrico
ICC/IF Mouse 10/10/2016
View

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample Testedmouse prostate
SpeciesMouse
Immunohistochemistry-Paraffin Luciferase NB100-1677
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reviewed by:
Anonymous
IHC-P Other 06/18/2016
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse brain tissue bearing human glioma cells expressing fire fly luciferase
SpeciesOther
Immunohistochemistry-Paraffin Luciferase NB100-1677
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reviewed by:
Anonymous
IHC-P Other 06/21/2013
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman tumor xenograft expressing firefly luciferase intracranially injected into a mouse brain
SpeciesOther
Lot28596
CommentsGreat antibody. Very clear signal, and little background, despite being polyclonal.

Blocking

Blocking DetailsBlocked in 0.2% bovine serum albumin + 2% normal donkey serum in PBS for 1.5 hours at room temp

Primary Anitbody

Dilution Ratio1:500 in 0.2% bovine serum albumin + 2% normal donkey serum in PBS overnight at 4C

Secondary Antibody

Secondary DescriptionDonkey anti goat alexa 488
Secondary Manufacturer Cat#Invitrogen A11055
Secondary Concentration1:500 in 0.2% BSA in PBS

Details

Detection NotesImaged on Nikon Eclipse Ci with Nuance multispectral imaging system (Perkin Elmer) Exposure time ~300ms
Fixation DetailsFixed ON in 10% neutral buffered formalin, processed in VIP6 tissue processor and paraffin embedded. 5um sections were dried ON
Wash Description3x in PBS 10 minutes each, room temp, plus second block step before secondary antibody. (Same as first block)

Comments

CommentsGreat antibody. Very clear signal, and little background, despite being polyclonal.
Immunocytochemistry Luciferase NB100-1677
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reviewed by:
Benjamin Dewals
ICC Other 10/04/2011
View

Summary

ApplicationImmunocytochemistry
Sample Testedvirus expressing luciferase transgene
SpeciesOther
Pub Med ID21593175

Blocking

Blocking DetailsBlocking Buffer: PBS with 0.1% BSA

Details

Fixation DetailsSample Preparation: PFA fixed, permeabilized

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Luciferase Antibody (NB100-1677). (Showing 1 - 2 of 2 FAQs).

  1. One of our customers would like additional information about your goat anti-luciferase (catalog #NB100-1677). They want to use this antibody for immunohistochemistry of paraffin-embedded sections, and would like to know if you recommend an antigen retrieval step? If so, which one is best?
    • For IHC-P with NB100-1677, we would recommend 10 mm citrate buffer (pH 6.0) based heat induced antigen retrieval at 95-99 C.
  2. Hello, can I use this antibody (NB100-1677) for immunohistochemistry on rat frozen brain sections?
    • This antibody will work in IHC on frozen and paraffin embedded tissues, provided that they express Firefly Luciferase from Photinus pyralis.

Secondary Antibodies

 

Isotype Controls

Additional Luciferase Products

Blogs on Luciferase.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

Luciferase: Shining a Light to See Inside Living Animal Models
The luciferase reporter is a valuable tool for research into physiology and disease. Light emitted from luciferase enables the monitoring of xenografted tumors, specific cell types, gene expression and pathogens within live animals over time using bio...  Read full blog post.

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Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

Alexandra Ambrico
10/10/2016
Application: ICC/IF
Species: Mouse

Anonymous
06/18/2016
Application: IHC-P
Species: Other

Anonymous
06/21/2013
Application: IHC-P
Species: Other

Bioinformatics

Uniprot