Luciferase Antibody

Images

 
Human tumor xenograft expressing firefly luciferase intracranially injected into a mouse brain. Image provided via product review by verified customer.
Lane 1: Luciferase, 50ng.Luciferase antibody at 1:1000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. ...read more
In vitro expression of Luciferase by 247Nluc+ recombinant strain of Alcelaphine herpesvirus 1 (AIHV-1). Epifluoresence expression of Luciferase by AIHV-1 infected syncytia. MDBK cells were infected with the WT (i to iv) ...read more
Analysis of human breast tumor xenograft expressing firefly luciferase subcutaneously injected into a mouse using Luciferase antibody. Primary antibody dilution 1:200. Image from verified customer review.
NIH3T3 cells. Cells (25,000/well) transfected with pLuc plasmid were stained with 20ug/ml NB100-1677 and 1:200 dilution of donkey anti-goat IgG-FITC (green). Cells were mounted with DAPI (blue) and visualized at 200X ...read more
Luciferase immunostaining was analyzed in tibias from ovx ERE-luciferase mice taken 24 h after a 17-E2 injection. Positive luciferase staining was identified in hypertrophic chondrocytes (hc) (A), lining cells (lc) (B), ...read more
Luciferase expression in mouse brain tissue bearing human glioma cells expressing fire fly luciferase. Image from verified customer review.
Western Blot of Goat anti-Luciferase antibody. Lane 1 Marker: Opal Pre-stained ladder

Product Details

Summary
Reactivity PpSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

Luciferase Antibody Summary

Description
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)]

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Immunogen
Luciferase [Photinus pyralis (Firefly)]
Specificity
No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Multi-step
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:200-1:1000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Frozen 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Western Blot 1:1000-1:5000
Application Notes
This antibody has been tested in Western Blot, IHC, and ELISA. Expect a band ~60kDa in appropriate cell lysates. Although not tested, this antibody would be useful in immunofluorescence, immunoprecipitation, immunocytochemistry, and most immunological methods requiring high titers and specificity.
Reviewed Applications
Read 7 Reviews rated 4.9
using
NB100-1677 in the following applications:

Publications
Read Publications using
NB100-1677 in the following applications:

Reactivity Notes

No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Multi-step

Alternate Names for Luciferase Antibody

  • ec 1.13.12.7
  • firefly luciferase
  • Fluc
  • LuC
  • Luciferase
  • luciferin 4 monooxygenase
  • Luciferin 4-monooxygenase

Background

Luciferase is a generic term for a group of oxidative enzymes used in bioluminescence. Firefly (Photinus pyralis) and bacterial luciferase enzymes are commonly used in assay systems such as cell viability assays, reporter gene assays, and for in vivo imaging. Bacterial luciferases are flavoenzymes composed of two subunits each encoded by the luxA and luxB genes, while the firefly luciferase is a single polypeptide specified by the luc gene (1). Firefly luciferase (theoretical molecular weight: 61 kDa) oxidizes the substrate luciferin to oxyluciferin in a bioluminescent reaction requiring Mg2+ and ATP (2,3). This reaction produces a flash of yellow-green light with an emission peak around 560nm that can be detected by a luminometer (3). Firefly luciferase has become one of the more widely used reporter proteins and is an excellent tool for the study of gene expression, given that the amount of light emitted is directly proportional to luciferase activity (4).

The luciferase assay is fast and sensitive, differentiating itself from the CAT (chloramphenicol acetyltransferase) assay because it does not require a radioactive substrate.

References

1. Eun, H. (1996). Marker/Reporter enzymes. Enzymology Primer for Recombinant DNA Technology, 567-645. doi:10.1016/b978-012243740-3/50011-9

2. McNabb, D. S., Reed, R., & Marciniak, R. A. (2005). Dual luciferase assay system for rapid assessment of gene expression in Saccharomyces cerevisiae. Eukaryotic Cell, 4(9), 1539-1549. doi:10.1128/ec.4.9.1539-1549.2005

3. Fraga, H. (2008). Firefly luminescence: A historical perspective and recent developments. Photochemical & Photobiological Sciences, 7(2), 146-158. doi:10.1039/b719181b

4. Younes, A., Lukyanenko, Y. O., Lyashkov, A. E., Lakatta, E. G., & Sollott, S. J. (2011). A bioluminescence method for direct measurement of phosphodiesterase activity. Analytical Biochemistry, 417(1), 36-40. doi:10.1016/j.ab.2011.05.036

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Luciferase Antibody (NB100-1677)(31)

We have publications tested in 1 confirmed species: Firefly.

We have publications tested in 9 applications: B/N, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IP, MiAr, WB.


Filter By Application
B/N
(1)
ICC/IF
(11)
IF/IHC
(4)
IHC
(5)
IHC-Fr
(3)
IHC-P
(3)
IP
(1)
MiAr
(1)
WB
(4)
All Applications
Filter By Species
Firefly
(2)
All Species
Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using NB100-1677 Applications Species
Rogg M, Maier JI, Helmstädter M et al. A YAP/TAZ-ARHGAP29-RhoA Signaling Axis Regulates Podocyte Protrusions and Integrin Adhesions Cells 2023-07-06 [PMID: 37443829] (WB)

Details:
Dilutions: 1:1000
WB
Escudero-Duch C, Mu�oz-Moreno L, Martin-Saavedra F et al. Remote control of transgene expression using noninvasive near-infrared irradiation Journal of photochemistry and photobiology. B, Biology 2023-03-22 [PMID: 36963296] (IHC) IHC
Melamed JR, Yerneni SS, Arral ML et al. Ionizable lipid nanoparticles deliver mRNA to pancreatic ? cells via macrophage-mediated gene transfer Science Advances 2023-01-27 [PMID: 36706177] (IHC) IHC
Lekk I, Cabrera-Cabrera F, Turconi G et al. Untranslated regions of brain-derived neurotrophic factor mRNA control its translatability and subcellular localization Journal of Biological Chemistry 2023-02-01 [PMID: 36639028] (B/N, IP, WB) B/N, IP, WB
Morita A, Nakayama M, Wang D et al. Frequent loss of metastatic ability in subclones of Apc, Kras, Tgfbr2, and Trp53 mutant intestinal tumor organoids Cancer science 2023-04-01 [PMID: 36576236] (IHC-P) IHC-P
Lee SY, Oh HR, Kim YH et al. Cerenkov luminescence imaging of interscapular brown adipose tissue using a TSPO-targeting PET probe in the UCP1 ThermoMouse Theranostics 2022-08-29 [PMID: 36168637] (IHC) IHC
Sullivan PM, Kumar R, Li W et al. FGFR4-Targeted Chimeric Antigen Receptors Combined with Anti-Myeloid Polypharmacy Effectively Treat Orthotopic Rhabdomyosarcoma Molecular Cancer Therapeutics 2022-10-07 [PMID: 35877472] (ICC/IF, IHC) ICC/IF, IHC
Trieu KG, Tsai SY, Eberl M et al. Basal cell carcinomas acquire secondary mutations to overcome dormancy and progress from microscopic to macroscopic disease Cell reports 2022-05-03 [PMID: 35508126] (ICC/IF) ICC/IF
Lu X, Fong KW, Gritsina G et al. HOXB13 suppresses de novo lipogenesis through HDAC3-mediated epigenetic reprogramming in prostate cancer Nature genetics [PMID: 35468964] (MiAr) MiAr
Noffsinger B, Witter A, Sheybani N Et al. Technical choices significantly alter the adaptive immune response against immunocompetent murine gliomas in a model-dependent manner Journal of neuro-oncology 2021-08-25 [PMID: 34432197]
Show All 31 Publications.

Reviews for Luciferase Antibody (NB100-1677) (7) 4.97

Average Rating: 4.9
(Based on 7 reviews)
We have 7 reviews tested in 3 species: Human, Mouse, Other.

Reviews using NB100-1677:
Filter by Applications
ICC
(2)
IHC-P
(3)
WB
(1)
Immunocytochemistry/Immunofluorescence
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(3)
Other
(3)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Luciferase NB100-1677
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5
reviewed by:
Yao Wang
ICC Mouse 12/30/2022
View

Summary

ApplicationImmunocytochemistry
Sample TestedE0771
SpeciesMouse
Lotnt

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.***
Immunohistochemistry-Paraffin Luciferase NB100-1677
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5
reviewed by:
Verified Customer
IHC-P Human 11/25/2022
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBreast cancer cells
SpeciesHuman
Lot47401
  5
reviewed by:
Verified Customer
WB Mouse 04/05/2018
View

Summary

ApplicationWestern Blot
Sample TestedMouse Bone marrow derived macrophage and osteoclast
SpeciesMouse
Lot35113
Immunocytochemistry/Immunofluorescence Luciferase NB100-1677
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5
reviewed by:
Alexandra Ambrico
Immunocytochemistry/Immunofluorescence Mouse 10/10/2016
View

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample Testedmouse prostate
SpeciesMouse
Immunohistochemistry-Paraffin Luciferase NB100-1677
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5
reviewed by:
Verified Customer
IHC-P Other 06/18/2016
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse brain tissue bearing human glioma cells expressing fire fly luciferase
SpeciesOther
Immunohistochemistry-Paraffin Luciferase NB100-1677
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5
reviewed by:
Verified Customer
IHC-P Other 06/21/2013
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman tumor xenograft expressing firefly luciferase intracranially injected into a mouse brain
SpeciesOther
Lot28596
Immunocytochemistry Luciferase NB100-1677
Enlarge
4
reviewed by:
Benjamin Dewals
ICC Other 10/04/2011
View

Summary

ApplicationImmunocytochemistry
Sample Testedvirus expressing luciferase transgene
SpeciesOther

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Luciferase Antibody (NB100-1677). (Showing 1 - 2 of 2 FAQs).

  1. One of our customers would like additional information about your goat anti-luciferase (catalog #NB100-1677). They want to use this antibody for immunohistochemistry of paraffin-embedded sections, and would like to know if you recommend an antigen retrieval step? If so, which one is best?
    • For IHC-P with NB100-1677, we would recommend 10 mm citrate buffer (pH 6.0) based heat induced antigen retrieval at 95-99 C.
  2. Hello, can I use this antibody (NB100-1677) for immunohistochemistry on rat frozen brain sections?
    • This antibody will work in IHC on frozen and paraffin embedded tissues, provided that they express Firefly Luciferase from Photinus pyralis.

Secondary Antibodies

 

Isotype Controls

Additional Luciferase Products

Research Areas for Luciferase Antibody (NB100-1677)

Find related products by research area.

Blogs on Luciferase.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

Luciferase: Shining a Light to See Inside Living Animal Models
The luciferase reporter is a valuable tool for research into physiology and disease. Light emitted from luciferase enables the monitoring of xenografted tumors, specific cell types, gene expression and pathogens within live animals over time using bio...  Read full blog post.

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Recent Reviews

4.9
5
6
4
1
3
0
2
0
1
0

Yao Wang
12/30/2022
Application: ICC
Species: Mouse

Verified Customer
11/25/2022
Application: IHC-P
Species: Human

 
Verified Customer
04/05/2018
Application: WB
Species: Mouse

Bioinformatics

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