Recombinant Renilla reniformis Luciferase His (N-Term) Protein

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SDS-Page: Recombinant Firefly Luciferase Protein [NBP2-59648] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity RrSpecies Glossary
Applications Func, PAGE, Bioactivity
Concentration
0.5 mg/ml

Order Details

Recombinant Renilla reniformis Luciferase His (N-Term) Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-311 of Renilla reniformis Luciferase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMTSKVY DPEQRKRMIT GPQWWARCKQ MNVLDSFINY YDSEKHAENA VIFLHGNAAS SYLWRHVVPH IEPVARCIIP DLIGMGKSGK SGNGSYRLLD HYKYLTAWFE LLNLPKKIIF VGHDWGACLA FHYSYEHQDK IKAIVHAESV VDVIESWDEW PDIEEDIALI KSEEGEKMVL ENNFFVETML PSKIMRKLEP EEFAAYLEPF KEKGEVRRPT LSWPREIPLV KGGKPDVVQI VRNYNAYLRA SDDLPKMFIE SDPGFFSNAI VEGAKKFPNT EFVKVKGLHF SQEDAPDEMG KYIKSFVERV LKNEQ

Details of Functionality
Specific activity is >1X10^9 light units/mg. One luciferase enzyme unit will produce one Relative Light Unit (RLU) at pH 7.5 at 25C.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
38.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Renilla reniformis (Sea pansy)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Renilla reniformis Luciferase His (N-Term) Protein

  • ec 1.13.12.7
  • Fluc
  • Luciferase
  • luciferin 4 monooxygenase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Other Available Formats

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