Luciferase Antibody (Luci 21 1-107) [PE]

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Product Details

Summary
Reactivity PpSpecies Glossary
Applications Flow
Clone
Luci 21 1-107
Clonality
Monoclonal
Host
Mouse
Conjugate
PE

Luciferase Antibody (Luci 21 1-107) [PE] Summary

Immunogen
Luciferase protein from Photinus pyralis (North American firefly). [UniProt# P08659]
Localization
Peroxisome
Specificity
Specific for Luciferase, recognizing a peptide consisting of the first 258 amino acids. Further epitope mapping has not been done at this time.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
This Luciferase (Luci 21 1-107) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry and Immunocytochemistry/Immunofluorescence. Western blot has been tested with Drosophila embryos. Purified Luciferase protein and Luciferase expressed in Drosophila adult co-migrate on Western blots with a band seen at ~61 kDa, representing Luciferase. Flow Cytometry has been reported in a customer review.

Reactivity Notes

Photinus pyralis (North American firefly). Luciferase recombinant reactivity in Mouse and Rat reported by a customer review.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Luciferase Antibody (Luci 21 1-107) [PE]

  • ec 1.13.12.7
  • Fluc
  • Luciferase
  • luciferin 4 monooxygenase

Background

Luciferase is a luminescent enzyme, mostly known for its role as a reporter protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Luciferase Antibody (NB600-307PE) (0)

There are no publications for Luciferase Antibody (NB600-307PE).
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Product General Protocols

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FAQs for Luciferase Antibody (NB600-307PE). (Showing 1 - 1 of 1 FAQ).

  1. Hello. Do you have a specific protocol for use of this antibody in FACS analysis? Luciferase Antibody (Luci 21 1-107) 0.1ml. It is uncojugated, so I am wondering if there is a specific protocol for labeling, etc. Thank you
    • The flow protocol used with this antibody NB600-307 was our general flow protocol. Since this antibody is unconjugated, you would want to use a conjugated mouse IgG secondary with this product in order to detect the antibody. We do also have this specific clone available in a directly conjugated PE form (NB600-307PE). If you are interested, I can inquire about a custom conjugate of another fluor or dye if you like. The other option would be for you to directly conjugate the antibody yourself. We do sell several conjugation kits that are very easy to use.

Secondary Antibodies

 

Isotype Controls

Additional Luciferase Products

Research Areas for Luciferase Antibody (NB600-307PE)

Find related products by research area.

Blogs on Luciferase.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

Luciferase: Shining a Light to See Inside Living Animal Models
The luciferase reporter is a valuable tool for research into physiology and disease. Light emitted from luciferase enables the monitoring of xenografted tumors, specific cell types, gene expression and pathogens within live animals over time using bio...  Read full blog post.

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