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![Western Blot: mCherry Antibody (857) [NBP2-43769] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of mCherry-tagged protein expressing 293T cells 5 % SDS-PAGE mCherry antibody [857] dilution: 1:1000.](http://images.novusbio.com/fullsize/mCherry-Antibody-857-Western-Blot-NBP2-43769-img0001.jpg "Western Blot: mCherry Antibody (857) [NBP2-43769] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of mCherry-tagged protein expressing 293T cells 5 % SDS-PAGE mCherry antibody [857] dilution: 1:1000.")
![Western Blot: Renilla Luciferase Antibody [NBP2-42914] - Renilla Luciferase antibody detects Renilla Luciferase protein by Western blot analysis. Non-transfected (-) and Renilla Luciferase -transfected (+, ) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Renilla Luciferase antibody at a dilution of 1:2000.](http://images.novusbio.com/fullsize/Renilla-Luciferase-Antibody-Western-Blot-NBP2-42914-img0001.jpg "Western Blot: Renilla Luciferase Antibody [NBP2-42914] - Renilla Luciferase antibody detects Renilla Luciferase protein by Western blot analysis. Non-transfected (-) and Renilla Luciferase -transfected (+, ) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Renilla Luciferase antibody at a dilution of 1:2000.")
![Non-transfected (-) and transfected (+) 293T whole cell extract (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with mCherry antibody [HL1482] (NBP3-25559) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25559_rabbit-mcherry-mab-hl1482-azide-and-bsa-free-western-blot-141120251842304.jpg "Non-transfected (-) and transfected (+) 293T whole cell extract (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with mCherry antibody [HL1482] (NBP3-25559) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: mCherry Antibody (HL1482) - Azide and BSA Free [NBP3-25559] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with mCherry antibody [HL1482] (NBP3-25559) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25559_rabbit-mcherry-mab-hl1482-azide-and-bsa-free-2120249342713.jpg)
![Dot Blot: Luciferase Antibody [NBP2-44165] - Dot Blot results of Rabbit anti-Luciferase Antibody. Dots are Luciferase (Photobacterium fischerii) at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: Rabbit anti-Luciferase. Secondary Antibody: Goat Anti-Rabbit IgG HRP.](http://images.novusbio.com/fullsize/Luciferase-Antibody-Dot-Blot-NBP2-44165-img0001.jpg "Dot Blot: Luciferase Antibody [NBP2-44165] - Dot Blot results of Rabbit anti-Luciferase Antibody. Dots are Luciferase (Photobacterium fischerii) at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: Rabbit anti-Luciferase. Secondary Antibody: Goat Anti-Rabbit IgG HRP.")
![Immunocytochemistry/Immunofluorescence: mCherry Antibody (844) [NBP2-43778] - mCherry antibody detects mCherry protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: mCherry stained by mCherry antibody diluted at 1:1000. Red: mCherry is expressed in the transfected cell. Blue: Hoechst 33342 staining. Scale bar= 10um.](http://images.novusbio.com/fullsize/mCherry-Antibody-844-Immunocytochemistry-Immunofluorescence-NBP2-43778-img0002.jpg "Immunocytochemistry/Immunofluorescence: mCherry Antibody (844) [NBP2-43778] - mCherry antibody detects mCherry protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: mCherry stained by mCherry antibody diluted at 1:1000. Red: mCherry is expressed in the transfected cell. Blue: Hoechst 33342 staining. Scale bar= 10um.")
![Western Blot: mCherry Antibody (844) [NBP2-43778] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of mCherry-tagged protein expressing 293T cells 5 % SDS-PAGE mCherry antibody [844] dilution: 1:1000.](http://images.novusbio.com/fullsize/mCherry-Antibody-844-Western-Blot-NBP2-43778-img0001.jpg)
![Western Blot: Luciferase Antibody [NB110-17348] - Lysates of HEK-293T cells overexpressing luciferase was separated on SDS-PAGE and probed with Anti-Luciferase The antibody was developed using Goat Anti-Rabbit IgG-Alkaline Phosphatase and a colorimetric substrate. Lanes 1. Antibody dilution 1:1,000 2. Antibody dilution 1:1,000 + luciferase immunizing peptide](http://images.novusbio.com/fullsize/Luciferase-Antibody-Western-Blot-NB110-17348-img0003.jpg "Western Blot: Luciferase Antibody [NB110-17348] - Lysates of HEK-293T cells overexpressing luciferase was separated on SDS-PAGE and probed with Anti-Luciferase The antibody was developed using Goat Anti-Rabbit IgG-Alkaline Phosphatase and a colorimetric substrate. Lanes 1. Antibody dilution 1:1,000 2. Antibody dilution 1:1,000 + luciferase immunizing peptide")
![Immunocytochemistry/Immunofluorescence: Luciferase Antibody [NB110-17348] - HEK-293T cells overexpressing luciferase were fixed and stained 10 ug/mL Anti-Luciferase. The antibody was developed using Goat Anti-Rabbit IgG, FITC conjugate.](http://images.novusbio.com/fullsize/Luciferase-Antibody-Immunocytochemistry-Immunofluorescence-NB110-17348-img0004.jpg)
![SDS-Page: Recombinant Luciferase (firefly) Protein [NBP3-18743] - 10% SDS-PAGE analysis](http://images.novusbio.com/fullsize/Recombinant-Luciferase-firefly-Protein-SDS-Page-NBP3-18743-img0001.jpg "SDS-Page: Recombinant Luciferase (firefly) Protein [NBP3-18743] - 10% SDS-PAGE analysis")
![Functional: Recombinant Luciferase (firefly) Protein [NBP3-18743] - An ATP dose response curve was performed in 50 ul reaction cocktails containing decreasing amounts of ATP, 0.2 ug Luciferase, and 15 nmol luciferin in a Tris-glycine buffer, pH 7.6 supplemented with 0.5 mM EDTA, 5 mM MgSO4 and 0.1% BSA. Luminescence was measured at 22C for 10 s in quadruplicates, using a GloMax 20/20 Luminometer.](http://images.novusbio.com/fullsize/Recombinant-Luciferase-firefly-Protein-Functional-NBP3-18743-img0002.jpg)
![Recombinant Luciferase (Firefly) Protein [NBP3-18743]—ATP is hydrolyzed, and light is emitted when firefly luciferase catalyzes the oxidation of D-luciferin. Reaction (1) is reversible, and the equilibrium lies far to the right. Reaction (2) is essentially irreversible. When ATP is the limiting reagent, the light emitted is proportional to the ATP present.](http://images.novusbio.com/fullsize/Recombinant-Luciferase-firefly-Protein-N-A-NBP3-18743-img0003.jpg)
![Western Blot: beta Galactosidase Antibody (BG-02) [NB500-334] - Analysis of purified E. coli beta-galactosidase sample; reduced sample, immunostained by mAb BG-02 and goat anti-mouse IgG (H+L)-HRP conjugate.](http://images.novusbio.com/fullsize/beta-Galactosidase-Antibody-BG-02-Western-Blot-NB500-334-img0001.jpg "Western Blot: beta Galactosidase Antibody (BG-02) [NB500-334] - Analysis of purified E. coli beta-galactosidase sample; reduced sample, immunostained by mAb BG-02 and goat anti-mouse IgG (H+L)-HRP conjugate.")
![SDS-Page: Recombinant Firefly Luciferase Protein [NBP1-48355]](http://images.novusbio.com/fullsize/Luciferase-firefly-Protein-SDS-Page-NBP1-48355-img0001.jpg "SDS-Page: Recombinant Firefly Luciferase Protein [NBP1-48355]")
![Western Blot: Luciferase (firefly) Antibody [NBP3-33161] - Western Blot of Luciferase (firefly) Antibody. Lane 1: Luciferase (Firefly). Load: 50 ng per lane. Primary antibody: Luciferase (firefly) Antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP goat secondary antibody at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for Luciferase.](http://images.novusbio.com/fullsize/nbp3-33161_goat-luciferase-firefly-pab-231202523584719.jpg "Western Blot: Luciferase (firefly) Antibody [NBP3-33161] - Western Blot of Luciferase (firefly) Antibody. Lane 1: Luciferase (Firefly). Load: 50 ng per lane. Primary antibody: Luciferase (firefly) Antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP goat secondary antibody at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for Luciferase.")